1. Activated alkyne- enabled turn- on click bioconjugation with cascade signal amplification for ultrafast and high- throughput antibiotic screening.
- Author
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Shuyi Lv, Chao Wang, Ke Xue, Jiaxin Wang, Minghui Xiao, Zhencheng Sun, Lei Han, Linqi Shi, and Chunlei Zhua
- Subjects
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MICROBIAL sensitivity tests , *ANTIBIOTICS - Abstract
Antimicrobial susceptibility testing plays a pivotal role in the discovery of new antibiotics. However, the development of simple, sensitive, and rapid assessment approaches remains challenging. Herein, we report an activated alkyne- based cascade signal ampli- fication strategy for ultrafast and high- throughput antibiotic screening. First of all, a novel water- soluble aggregation- induced emission (AIE) luminogen is synthesized, which contains an activated alkyne group to enable fluorescence turn- on and metal- free click bioconjugation under physiological conditions. Taking advantage of the in- house established method for bacterial lysis, a number of clickable biological substances (i.e., bacterial solutes and debris) are released from the bacterial bodies, which remarkably increases the quantity of analytes. By means of the activated alkyne- mediated turn- on click bioconjugation, the system fluorescence signal is significantly amplified due to the increased labeling sites as well as the AIE effect. Such a cascade signal amplification strategy efficiently improves the detection sensitivity and thus enables ultrafast antimicrobial susceptibility assessment. By integration with a microplate reader, this approach is further applied to high- throughput antibiotic screening. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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