1. Majority of CD4 + T cells from peripheral blood of HIV-1–infected individuals contain only one HIV DNA molecule
- Author
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Frank Maldarelli, Jianbo Chen, Johan Brännström, Wei-Shau Hu, Sarah Palmer, Hans Gaines, Martin S. King, Wei Shao, John M. Coffin, John W. Mellors, Jan Albert, Mary F. Kearney, Lina Josefsson, and Barbro Mäkitalo
- Subjects
CD4-Positive T-Lymphocytes ,Time Factors ,Sequence analysis ,HIV Infections ,Biology ,Virus ,chemistry.chemical_compound ,Proviruses ,In vivo ,Phylogenetics ,Genetic variation ,Humans ,Phylogeny ,DNA Primers ,Recombination, Genetic ,Multidisciplinary ,Base Sequence ,Genetic Variation ,RNA ,Sequence Analysis, DNA ,Biological Sciences ,Viral Load ,Virology ,chemistry ,Chronic Disease ,DNA, Viral ,HIV-1 ,RNA, Viral ,Viral load ,DNA - Abstract
Neither the number of HIV-1 proviruses within individual infected cells in HIV-1–infected patients nor their genetic relatedness within individual infected cells and between cells and plasma virus are well defined. To address these issues we developed a technique to quantify and genetically characterize HIV-1 DNA from single infected cells in vivo. Analysis of peripheral blood CD4 + T cells from nine patients revealed that the majority of infected cells contain only one copy of HIV-1 DNA, implying a limited potential for recombination in virus produced by these cells. The genetic similarity between HIV populations in CD4 + T cells and plasma implies ongoing exchange between these compartments both early and late after infection.
- Published
- 2011
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