10 results on '"Paolo de Coppi"'
Search Results
2. Fetoscopic insufflation of heated‐humidified carbon dioxide during simulated spina bifida repair is safe under controlled anesthesia in the fetal lamb
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Luc Joyeux, David Basurto, Tom Bleeser, Lennart Van der Veeken, Simen Vergote, Yada Kunpalin, Lucas Trigo, Enrico Corno, Felix R. De Bie, Paolo De Coppi, Sebastien Ourselin, Frank Van Calenbergh, Stuart B. Hooper, Steffen Rex, and Jan Deprest
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safety ,fetal surgery ,Hot Temperature ,Sheep ,carbon dioxide insufflation ,Fetoscopy ,myelomeningocele ,Obstetrics and Gynecology ,Humidity ,Insufflation ,fetoscopy ,Carbon Dioxide ,fetal hypercapnia ,Pregnancy ,Animals ,Anesthesia ,Female ,fetal acidosis ,Spina bifida ,Spinal Dysraphism ,Genetics (clinical) - Abstract
OBJECTIVE: To assess the safety of Partial-Amniotic-Insufflation-of-heated-humidified-CO2 (hPACI) during fetoscopic spina bifida repair (fSB-repair). METHOD: A simulated fSB-repair through an exteriorized uterus under hPACI was performed in 100-day fetal lambs (term = 145 days) under a laboratory anesthesia protocol (n = 5; group 1) which is known to induce maternal-fetal acidosis and hypercapnia. Since these may not occur clinically, we applied a clinical anesthesia protocol (n = 5; group 2), keeping maternal parameters within physiological conditions, that is, controlled maternal arterial carbon dioxide (CO2) pressure (pCO2 = 30 mmHg), blood pressure (≥67 mmHg), and temperature (37.1-39.8°C). Our superiority study used fetal pH as the primary outcome. RESULTS: Compared to group 1, controlled anesthesia normalized fetal pH (7.23 ± 0.02 vs. 7.36 ± 0.02, p
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- 2022
3. Incidence and patterns of abnormal corpus callosum in fetuses with isolated spina bifida aperta
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Luc De Catte, Ioannis Papastefanou, Anna L. David, Jute Richter, Adalina Sacco, Paolo De Coppi, Emma Bredaki, Fred Ushakov, Sebastien Ourselin, Katrien Jansen, Jan Deprest, Yada Kunpalin, and Francesca Russo
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Adult ,0301 basic medicine ,medicine.medical_treatment ,Splenium ,Gestational Age ,030105 genetics & heredity ,Corpus callosum ,Article ,Cohort Studies ,Lesion ,03 medical and health sciences ,Fetus ,0302 clinical medicine ,Pregnancy ,medicine ,Humans ,Genetics (clinical) ,Retrospective Studies ,030219 obstetrics & reproductive medicine ,Spina bifida ,Fetal surgery ,business.industry ,Incidence ,Rostrum ,Obstetrics and Gynecology ,Gestational age ,Anatomy ,medicine.disease ,Spina Bifida Cystica ,Female ,Agenesis of Corpus Callosum ,medicine.symptom ,business - Abstract
OBJECTIVE: To determine the incidence and characterise corpus callosum (CC) abnormalities in fetuses with spina bifida aperta (SBA) between 18 and 26 weeks of gestation. METHODS: This was a retrospective study on fetuses with isolated SBA and who were assessed for fetal surgery. Digitally stored ultrasound images of the brain were reviewed for the presence/absence of the CC, and the length and diameter of its constituent parts (rostrum, genu, body and splenium). We used regression analysis to determine the relationship between CC abnormalities and gestational age, head circumference, ventricle size, lesion level and lesion type. RESULTS: Nearly three-quarters of fetuses with isolated SBA had an abnormal CC (71.7%, 76/106). Partial agenesis was most common in the splenium (18.9%, 20/ 106) and the rostrum (13.2%, 14/106). The most common abnormal pattern was of a short CC with normal diameter throughout. Of note, 20.8% (22/106) had a hypoplastic genu and 28.3% (30/106) had a thick body part. Larger lateral ventricle size was associated with partial agenesis of the CC (odds ratio [OR]: 0.14, p < 0.001) and inversely associated with a shorter CC (OR: 2.60, p < 0.01). CONCLUSION: An abnormal CC is common in fetuses with isolated SBA who are referred for fetal surgery.
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- 2021
4. Getting earlier, smaller and regenerative: The next 10 years of in utero spina bifida repair
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Dominic Thompson and Paolo De Coppi
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medicine.medical_specialty ,Meningomyelocele ,business.industry ,Spina bifida ,Obstetrics ,Obstetrics and Gynecology ,medicine.disease ,Neurosurgical Procedures ,Spina bifida repair ,In utero ,Humans ,Medicine ,In utero surgery ,business ,Spinal Dysraphism ,Fetal therapy ,Genetics (clinical) - Published
- 2021
5. Preclinical stem cell therapy in fetuses with myelomeningocele: A systematic review and meta-analysis
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Anna L. David, Mattia F. M. Gerli, Jan Bosteels, Paolo De Coppi, Sebastien Ourselin, Jan Deprest, Yada Kunpalin, Silvia Perin, and Sindhu Subramaniam
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Oncology ,medicine.medical_specialty ,Amniotic fluid ,Meningomyelocele ,SURGERY ,medicine.medical_treatment ,SPINA-BIFIDA ,FETAL LAMB MODEL ,IN-UTERO ,INTRAAMNIOTIC INJECTION ,RAT FETUSES ,Article ,DELIVERY ,03 medical and health sciences ,0302 clinical medicine ,Fetus ,Pregnancy ,Internal medicine ,CORD ,Medicine ,Animals ,Genetics (clinical) ,Genetics & Heredity ,Science & Technology ,Chi-Square Distribution ,Sheep ,business.industry ,Fetal surgery ,Mesenchymal stem cell ,Obstetrics & Gynecology ,Obstetrics and Gynecology ,General Medicine ,Stem-cell therapy ,3. Good health ,Rats ,In utero ,030220 oncology & carcinogenesis ,Meta-analysis ,CHICK-EMBRYOS ,NEURAL-TUBE DEFECTS ,Female ,Stem cell ,business ,Life Sciences & Biomedicine ,030217 neurology & neurosurgery ,Stem Cell Transplantation - Abstract
OBJECTIVE: We performed a systematic review to summarize the efficacy and safety of in utero stem cells application in preclinical models with myelomeningocele (MMC). METHODS: The study was registered with PROSPERO (CRD42019160399). We searched MEDLINE, Embase, Web of Science, Scopus and CENTRAL for publications articles on stem cell therapy in animal fetuses with MMC until May 2020. Publication quality was assessed by the SYRCLE's tool. Meta-analyses were pooled if studies were done in the same animal model providing similar type of stem cell used and outcome measurements. Narrative synthesis was performed for studies that could not be pooled. RESULTS: Nineteen and seven studies were included in narrative and quantitative syntheses, respectively. Most used mesenchymal stem cells (MSCs) and primarily involved ovine and rodent models. Both intra-amniotic injection of allogeneic amniotic fluid (AF)-MSCs in rat MMC model and the application of human placental (P)-MSCs to the spinal cord during fetal surgery in MMC ovine model did not compromise fetal survival rates at term (rat model, relative risk [RR] 1.03, 95% CI 0.92-1.16; ovine model, RR 0.94, 95% CI 0.78-1.13). A single intra-amniotic injection of allogeneic AF-MSCs into rat MMC model was associated with a higher rate of complete defect coverage compared to saline injection (RR 16.35, 95% CI 3.27-81.79). The incorporation of human P-MSCs as a therapeutic adjunct to fetal surgery in the ovine MMC model significantly improved sheep locomotor rating scale after birth (mean difference 5.18, 95% CI 3.36-6.99). CONCLUSIONS: Stem cell application during prenatal period in preclinical animal models is safe and effective. ispartof: PRENATAL DIAGNOSIS vol:41 issue:3 pages:283-300 ispartof: location:England status: published
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- 2020
6. A systematic review and meta-analysis on fetal ovarian cysts: impact of size, appearance and prenatal aspiration
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Paolo De Coppi, Spyros Bakalis, Anna L. David, Athanasios Tyraskis, and Simon Eaton
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Fetus ,medicine.medical_specialty ,Pregnancy ,030219 obstetrics & reproductive medicine ,Ovarian cyst ,business.industry ,Ovarian torsion ,Obstetrics and Gynecology ,Subgroup analysis ,medicine.disease ,Surgery ,03 medical and health sciences ,0302 clinical medicine ,In utero ,Meta-analysis ,parasitic diseases ,medicine ,Cyst ,030212 general & internal medicine ,business ,Genetics (clinical) - Abstract
The objective of the study is to compare outcomes of ultrasound-guided aspiration of fetal ovarian cysts with conservative management. METHOD: A systematic review of MEDLINE and Web of Science included studies reporting outcomes (prenatal and postnatal torsion, spontaneous resolution and surgery) of fetuses with ovarian cysts. Subgroup analysis was performed according to cyst diameter at diagnosis and cysts ≥40 mm. RESULTS: Ninety-two non-randomised studies reported on 380 cysts (324 observed and 56 aspirated in utero) in 365 fetuses. All studies were case reports or series with high heterogeneity and risk of bias. The overall spontaneous resolution rate of conservatively managed cysts was 46%, yet decreased with increasing cyst size. Risk of prenatal ovarian torsion in conservatively managed cases depended on cyst size and was particularly important in the range 30 to 59 mm (15–34%). The rate of prenatal torsion in simple cysts ≥40 mm was lower in aspirated than conservatively managed cysts (0% vs 10%, p = 0.03). Aspirated cysts had lower rates of postnatal surgery (7%) compared with conservatively managed cysts (49%, p < 0.001). CONCLUSION: Cysts 30 to 59 mm were at highest risk of torsion. Simple cysts >40 mm had lower rates of torsion when aspirated prenatally. Randomised studies and safety data are needed prior to routine prenatal ovarian cyst aspiration.
- Published
- 2017
7. The use of human amniotic fluid stem cells as an adjunct to promote pulmonary development in a rabbit model for congenital diaphragmatic hernia
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Shea Carter, Jaan Toelen, Michela Pozzobon, Erik Verbeken, Jan Deprest, Pieter-Jan Vandersloten, Francesca Russo, Philip DeKoninck, Jute Richter, Paolo De Coppi, and Xenia Roubliova
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Fetus ,Pathology ,medicine.medical_specialty ,Lung ,Bronchiole ,Amniotic fluid ,business.industry ,Obstetrics and Gynecology ,Congenital diaphragmatic hernia ,medicine.disease ,Pulmonary hypoplasia ,medicine.anatomical_structure ,Parenchyma ,medicine ,Stem cell ,business ,Genetics (clinical) - Abstract
Objective This study aimed to evaluate the potential benefit of intra-tracheal injection of human amniotic fluid stem cells (hAFSC) on pulmonary development combined with TO in a rabbit model for CDH. Methods In time-mated pregnant does a left diaphragmatic defect was created at d23 (term = 31). At d28, previously operated fetuses were assigned to either TO and injection with 70 μL of phosphate buffered saline (PBS) or 1.0 × 106 c-Kit positive hAFSC expressing LacZ or were left untouched (CDH). Harvesting was done at d31 to obtain their lung-to-body weight ratio (LBWR), airway and vascular lung morphometry, X-gal staining and immunohistochemistry for Ki67 and surfactant protein-B (SP-B). Results CDH-induced pulmonary hypoplasia is countered by TO + PBS, this reverses LBWR, mean terminal bronchiole density (MTBD) and medial thickness to normal. The additional injection of hAFSC decreases MTBD and results in a non-significant decrease in muscularization of intra-acinary vessels. There were no inflammatory changes and LacZ positive hAFSC were dispersed throughout the lung parenchyma 4 days after injection. Conclusion HAFSC exert an additional effect on TO leading to a decrease in MTBD, a measure of alveolar number surrounding the terminal bronchioles, without signs of toxicity. © 2015 John Wiley & Sons, Ltd.
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- 2015
8. Routine clonal expansion of mesenchymal stem cells derived from amniotic fluid for perinatal applications
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Silvia Zia, Paolo De Coppi, Marina Gabriela Monteiro Carvalho Mori da Cunha, Philip DeKoninck, Jan Deprest, and Jaan Toelen
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Pathology ,medicine.medical_specialty ,Amniotic fluid ,medicine.diagnostic_test ,Cluster of differentiation ,business.industry ,Mesenchymal stem cell ,Obstetrics and Gynecology ,Cell therapy ,medicine.anatomical_structure ,Cord blood ,Amniocentesis ,medicine ,Bone marrow ,Stem cell ,business ,Genetics (clinical) - Abstract
Introduction Stem cells (SCs) isolated from amniotic fluid (AF) are a promising source for autologous perinatal cell therapy. The aim of this study was to develop a routine isolation, selection, and expansion protocol of clonal SC lines from redundant clinical amniocentesis samples. Materials and methods Amniotic fluids were collected between 15 and 22 weeks of gestation, and SCs were isolated by CD117-based and mechanical selection protocols. SCs were characterized by mesenchymal SC marker expression and differentiation protocols. Cells were manipulated with a lentiviral vector system expressing the β-galactosidase reporter gene and were injected into immunodeficient newborn mouse pups. Qualitative assessment was performed to detect the infused cells after 1 week. Results A total of 78 clonal AF SC populations were successfully isolated by mechanical selection from 21 consecutive amniocentesis samples. They were positive for mesenchymal SC cluster of differentiation markers and could be differentiated into the different lineages. SCs were stably labeled using β-galactosidase and were detected in the lungs and hearts of the neonatal mice. Conclusion We demonstrate that mesenchymal SCs can be routinely isolated and clonally expanded from mid-gestation human AF using mechanical isolation. They can easily be transduced and be tested for perinatal treatment in animal models. © 2013 John Wiley & Sons, Ltd.
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- 2013
9. A systematic review and meta-analysis on fetal ovarian cysts: impact of size, appearance and prenatal aspiration
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Athanasios, Tyraskis, Spyros, Bakalis, Anna L, David, Simon, Eaton, and Paolo, De Coppi
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Fetal Diseases ,Ovarian Cysts ,Torsion Abnormality ,Treatment Outcome ,Pregnancy ,Risk Factors ,MEDLINE ,Humans ,Female ,Ovarian Diseases ,Suction ,Ultrasonography, Prenatal - Abstract
The objective of the study is to compare outcomes of ultrasound-guided aspiration of fetal ovarian cysts with conservative management.A systematic review of MEDLINE and Web of Science included studies reporting outcomes (prenatal and postnatal torsion, spontaneous resolution and surgery) of fetuses with ovarian cysts. Subgroup analysis was performed according to cyst diameter at diagnosis and cysts ≥40 mm.Ninety-two non-randomised studies reported on 380 cysts (324 observed and 56 aspirated in utero) in 365 fetuses. All studies were case reports or series with high heterogeneity and risk of bias. The overall spontaneous resolution rate of conservatively managed cysts was 46%, yet decreased with increasing cyst size. Risk of prenatal ovarian torsion in conservatively managed cases depended on cyst size and was particularly important in the range 30 to 59 mm (15-34%). The rate of prenatal torsion in simple cysts ≥40 mm was lower in aspirated than conservatively managed cysts (0% vs 10%, p = 0.03). Aspirated cysts had lower rates of postnatal surgery (7%) compared with conservatively managed cysts (49%, p 0.001).Cysts 30 to 59 mm were at highest risk of torsion. Simple cysts40 mm had lower rates of torsion when aspirated prenatally. Randomised studies and safety data are needed prior to routine prenatal ovarian cyst aspiration. © 2017 John WileySons, Ltd.
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- 2016
10. Routine clonal expansion of mesenchymal stem cells derived from amniotic fluid for perinatal applications
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Silvia, Zia, Jaan, Toelen, Marina, Mori da Cunha, Philip, Dekoninck, Paolo, de Coppi, and Jan, Deprest
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Pluripotent Stem Cells ,Cell Culture Techniques ,Mesenchymal Stem Cells ,Mice, SCID ,Amniotic Fluid ,Mesenchymal Stem Cell Transplantation ,Clone Cells ,Mice ,Animals, Newborn ,Pregnancy ,Prenatal Diagnosis ,Animals ,Humans ,Female ,Cells, Cultured ,Cell Proliferation - Abstract
Stem cells (SCs) isolated from amniotic fluid (AF) are a promising source for autologous perinatal cell therapy. The aim of this study was to develop a routine isolation, selection, and expansion protocol of clonal SC lines from redundant clinical amniocentesis samples.Amniotic fluids were collected between 15 and 22 weeks of gestation, and SCs were isolated by CD117-based and mechanical selection protocols. SCs were characterized by mesenchymal SC marker expression and differentiation protocols. Cells were manipulated with a lentiviral vector system expressing the β-galactosidase reporter gene and were injected into immunodeficient newborn mouse pups. Qualitative assessment was performed to detect the infused cells after 1 week.A total of 78 clonal AF SC populations were successfully isolated by mechanical selection from 21 consecutive amniocentesis samples. They were positive for mesenchymal SC cluster of differentiation markers and could be differentiated into the different lineages. SCs were stably labeled using β-galactosidase and were detected in the lungs and hearts of the neonatal mice.We demonstrate that mesenchymal SCs can be routinely isolated and clonally expanded from mid-gestation human AF using mechanical isolation. They can easily be transduced and be tested for perinatal treatment in animal models.
- Published
- 2013
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