1. Application of Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry for Identification of Coagulase-negative Staphylococci Isolated from Milk of Cows with Subclinical Mastitis
- Author
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B. Furmaga, Paweł Łyp, Maciej Skrzypczak, J. Zietek, M. Bochniarz, Łukasz Adaszek, Stanisław Winiarczyk, Tomasz Banach, and W. Wawron
- Subjects
0301 basic medicine ,Desorption ionization ,Staphylococcus ,030106 microbiology ,Analytical chemistry ,Matrix assisted laser desorption ionization time of flight ,Mass spectrometry ,03 medical and health sciences ,Staphylococcus sciuri ,medicine ,Animals ,Subclinical mastitis ,Mastitis, Bovine ,Phylogeny ,Chromatography ,General Veterinary ,biology ,Chemistry ,food and beverages ,General Medicine ,Staphylococcal Infections ,biology.organism_classification ,medicine.disease ,Mastitis ,Milk ,030104 developmental biology ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Cattle ,Female ,Coagulase - Abstract
The aim of this study was to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of coagulase-negative staphylococci (CNS) isolated from the milk of cows with subclinical mastitis. The study material consisted of 33 isolates of CNS, identified by the results of API Staph tests, obtained from the milk of cows with subclinical mastitis. Based on the spectra analyses, MALDI-TOF MS tests of 33 bacterial samples allowed identification of the microorganisms in 27 cases (81.8%). The most frequent cause of subclinical mastitis was found to beStaphylococcussciuri (39%), whileS. vitulinuswas detected in 15% of the milk samples. The results obtained indicate that MALDI-TOF MS can be used for the identification of CNS isolated from bovine mastitis as a method supplementary to biochemical tests.
- Published
- 2016