The Biosite Triage D-dimer is a new, rapid assay utilizing EDTA whole blood or plasma that can be used at the point-of-care or in a central laboratory. Most coagulation assays, including many D-dimer assays, require citrated plasma, but the Triage D-dimer has not been previously studied using citrated plasma specimens. Seventy-four citrated plasma specimens on which D-dimer testing was requested for clinical testing were assayed on both the VIDAS and the Triage D-dimer systems. A subset of 29 of these specimens also had an EDTA specimen collected simultaneously and were assayed using the Triage D-dimer system. Diagnostic agreement between Triage citrate and VIDAS citrate, Triage EDTA and VIDAS citrate, and Triage citrate and Triage EDTA was ≥93%. Correlation coefficients (R2) were 0.86 for Triage citrate versus VIDAS, 0.93 for Triage EDTA versus VIDAS, and 0.96 for Triage citrate versus Triage EDTA. Diagnostic agreement was not changed after adjusting for the dilutional effects of citrate. Bland-Altman bias plots showed no bias between Triage citrate and Triage EDTA results if citrate results were adjusted for the dilutional effects of citrate. Triage results, whether EDTA, citrate, or adjusted citrate, tended to underestimate the VIDAS result at the low end and overestimate at the high end. In conclusion, the results suggest that citrate is a suitable alternative to EDTA for the Triage system. These data suggest that the Triage D-dimer compares favorably with the VIDAS, albeit the cutoff values are different. Further studies are warranted to confirm the possibility that Triage D-dimer is a suitable alternative to VIDAS.