1. Sialylation of campylobacter jejuni lipo-oligosaccharides: Impact on phagocytosis and cytokine production in mice
- Author
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Huizinga, R. (Ruth), Easton, A.S. (Alistair), Donachie, A.M. (Anne), Guthrie, J. (Jim), Rijs, W. (Wouter) van, Heikema, A.P. (Astrid), Boon, L. (Louis), Samsom, J.N. (Janneke), Jacobs, B.C. (Bart), Willison, H.J. (Hugh), Goodyear, C.S. (Carl), Huizinga, R. (Ruth), Easton, A.S. (Alistair), Donachie, A.M. (Anne), Guthrie, J. (Jim), Rijs, W. (Wouter) van, Heikema, A.P. (Astrid), Boon, L. (Louis), Samsom, J.N. (Janneke), Jacobs, B.C. (Bart), Willison, H.J. (Hugh), and Goodyear, C.S. (Carl)
- Abstract
Background: Guillain-Barré syndrome (GBS) is a post-infectious polyradiculoneuropathy, frequently associated with antecedent Campylobacter jejuni (C. jejuni) infection. The presence of sialic acid on C. jejuni lipo-oligosaccharide (LOS) is considered a risk factor for development of GBS as it crucially determines the structural homology between LOS and gangliosides, explaining the induction of cross-reactive neurotoxic antibodies. Sialylated C. jejuni are recognised by TLR4 and sialoadhesin; however, the functional implications of these interactions in vivo are unknown. Methodology/Principal Findings: In this study we investigated the effects of bacterial sialylation on phagocytosis and cytokine secretion by mouse myeloid cells in vitro and in vivo. Using fluorescently labelled GM1a/GD1a ganglioside-mimicking C. jejuni strains and corresponding (Cst-II-mutant) control strains lacking sialic acid, we show that sialylated C. jejuni was more efficiently phagocytosed in vitro by BM-MΦ, but not by BM-DC. In addition, LOS sialylation increased the production of IL-10, IL-6 and IFN-β
- Published
- 2012
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