19 results on '"wenhui zhang"'
Search Results
2. Characteristics of Carbapenem-resistant Klebsiella pneumoniae in sewage from a tertiary hospital in Jilin Province, China.
- Author
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Mingwei Liu, Lin Zheng, Lingwei Zhu, Gejin Lu, Hongru Guo, Jiayao Guan, Jie Jing, Shiwen Sun, Ying Wang, Zixian Wang, Yang Sun, Xue Ji, Bowen Jiang, Jun Liu, Wenhui Zhang, and Xuejun Guo
- Subjects
Medicine ,Science - Abstract
Carbapenem-resistant Klebsiella pneumoniae (CRKP) infection is a serious problem in hospitals worldwide. We monitored a tertiary hospital in Changchun, Jilin Province, China, and found that CRKP was the major species among the carbapenem-resistant isolates in sewage. Subsequently, we evaluated the drug susceptibility, resistance genes, virulence genes, outer pore membrane protein-related genes (OmpK35 & OmpK 36), multi-locus sequence typing and replicons, biofilm formation capabilities, and resistance to chlorine-containing disinfectants among KP isolates. Identification of drug sensitivity, multiple resistance profiles were observed including 77 (82.80%) multidrug resistant (MDR), 16 (17.20%) extensive drug resistant (XDR). Some antibiotic resistance genes were detected, the most prevalent carbapenemase gene was blaKPC, and 16 resistance genes were associated with other antibiotics. In addition, 3 (3.23%) CRKP isolates demonstrated loss of OmpK-35 and 2 (2.15%) demonstrated loss of OmpK-36. In the detection of multi-locus sequence typing (MLST), 11 ST11 isolates carried virulence genes. The most common replicon type was IncFII. Biofilm-forming capabilities were demonstrated by 68.8% of the isolates, all of which were resistant to chlorine-containing disinfectants. The results of the study showed that antibiotic-resistant isolates, especially CRKP, could resist disinfectants in hospital wastewater, and improper treatment of hospital wastewater may lead to the spread of drug-resistant bacteria and their genes. Thus, these bacteria must be eliminated before being discharged into the municipal sewage system.
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- 2023
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3. Protective effects of tiotropium alone or combined with budesonide against cadmium inhalation induced acute neutrophilic pulmonary inflammation in rats.
- Author
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Shiwei Zhao, Qi Yang, Zhixi Yu, You Lv, Jianming Zhi, Pascal Gustin, and Wenhui Zhang
- Subjects
Medicine ,Science - Abstract
As a potent bronchodilator, the anti-inflammatory effects of tiotropium and its interaction with budesonide against cadmium-induced acute pulmonary inflammation were investigated. Compared to values obtained in rats exposed to cadmium, cytological analysis indicated a significant decrease of total cell and neutrophil counts and protein concentration in bronchoalveolar lavage fluid (BALF) in rats pretreated with tiotropium (70μg/15ml or 350μg/15ml). Zymographic tests showed a decrease of MMP-2 activity in BALF in rats pretreated only with high concentration of tiotropium. Histological examination revealed a significant decrease of the severity and extent of inflammatory lung injuries in rats pretreated with both tested concentrations of tiotropium. Though tiotropium (70μg/15ml) or budesonide (250μg/15ml) could not reduce cadmium-induced bronchial hyper-responsiveness, their combination significantly decreased bronchial contractile response to methacholine. These two drugs separately decreased the neutrophil number and protein concentration in BALF but no significant interaction was observed when both drugs were combined. Although no inhibitory effects on MMP-2 and MMP-9 was observed in rats pretreated with budesonide alone, the combination with the ineffective dose of tiotropium induced a significant reduction on these parameters. The inhibitory effect of tiotropium on lung injuries was not influenced by budesonide which alone induced a limited action on the severity and extent of inflammatory sites. Our findings show that tiotropium exerts anti-inflammatory effects on cadmium-induced acute neutrophilic pulmonary inflammation. The combination of tiotropium with budesonide inhibits cadmium-induced inflammatory injuries with a synergistic interaction on MMP-2 and MMP-9 activity and airway hyper-responsiveness.
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- 2018
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4. The safety and efficacy of second-generation cryoballoon ablation plus catheter ablation for persistent atrial fibrillation: A systematic review and meta-analysis.
- Author
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Mengjiao Shao, Luxiang Shang, Jia Shi, Yang Zhao, Wenhui Zhang, Ling Zhang, Yaodong Li, Baopeng Tang, and Xianhui Zhou
- Subjects
Medicine ,Science - Abstract
BACKGROUND:Growing evidence suggests that second-generation cryoballoon ablation (2G-CB) is effective in patients with persistent atrial fibrillation (PerAF). The cornerstone of atrial fibrillation (AF) ablation is pulmonary vein isolation (PVI). The purpose of this study was to summarize the available data on the safety and mid-term (≥ 12 months) effectiveness of a 'PVI-only' strategy vs. a 'PVI-plus' strategy using 2G-CB in patients with PerAF. METHODS:We searched the PubMed, EMBASE and Cochrane library databases for studies on 2G-CB for PerAF. Group analysis was based on the ablation approach: 'PVI-only' versus 'PVI-plus', the latter of which involved PVI plus other substrate modifications. Studies showing clinical success rates at a follow-up (FU) of ≥ 12 months were included. Complication rates were also assessed. Data were analyzed by applying a fixed effects model. RESULTS:A total of 879 patients from 5 studies were analyzed. After a mid-term FU of 27 months, the overall success rate of 2G-CB for PerAF was 66.1%. In the 'PVI-plus' group, the success rate was 73.8%. In the 'PVI-only' group, the success rate was 53.6%. No heterogeneity was noted among studies (I2 = 0.0%, P = 0.82). Complications occurred in 5.2% of patients (P = 0.93), and the rate of phrenic nerve (PN) injury was 2.8% (P = 0.14). Vascular assess complications were the most frequent at 1.6% (P = 0.33). No death or myocardial infarction was reported. CONCLUSION:'PVI-plus' involving 2G-CB seems to be safe and effective for treating PerAF.
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- 2018
- Full Text
- View/download PDF
5. Evaluating the influential priority of the factors on insurance loss of public transit.
- Author
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Wenhui Zhang, Yongmin Su, Ruimin Ke, and Xinqiang Chen
- Subjects
Medicine ,Science - Abstract
Understanding correlation between influential factors and insurance losses is beneficial for insurers to accurately price and modify the bonus-malus system. Although there have been a certain number of achievements in insurance losses and claims modeling, limited efforts focus on exploring the relative role of accidents characteristics in insurance losses. The primary objective of this study is to evaluate the influential priority of transit accidents attributes, such as the time, location and type of accidents. Based on the dataset from Washington State Transit Insurance Pool (WSTIP) in USA, we implement several key algorithms to achieve the objectives. First, K-means algorithm contributes to cluster the insurance loss data into 6 intervals; second, Grey Relational Analysis (GCA) model is applied to calculate grey relational grades of the influential factors in each interval; in addition, we implement Naive Bayes model to compute the posterior probability of factors values falling in each interval. The results show that the time, location and type of accidents significantly influence the insurance loss in the first five intervals, but their grey relational grades show no significantly difference. In the last interval which represents the highest insurance loss, the grey relational grade of the time is significant higher than that of the location and type of accidents. For each value of the time and location, the insurance loss most likely falls in the first and second intervals which refers to the lower loss. However, for accidents between buses and non-motorized road users, the probability of insurance loss falling in the interval 6 tends to be highest.
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- 2018
- Full Text
- View/download PDF
6. CRISPR/Cas9 mediated chicken Stra8 gene knockout and inhibition of male germ cell differentiation.
- Author
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Yani Zhang, Yingjie Wang, Qisheng Zuo, Dong Li, Wenhui Zhang, Fei Wang, Yanqin Ji, Jing Jin, Zhenyu Lu, Man Wang, Chen Zhang, and Bichun Li
- Subjects
Medicine ,Science - Abstract
An efficient genome editing approach had been established to construct the stable transgenic cell lines in the domestic chicken (Gallus gallus domesticus) at present. Our objectives were to investigate gene function in the differentiation process of chicken embryonic stem cells (ESCs) into spermatogonial stem cells(SSCs). Three guides RNA (gRNAs) were designed to knockout the Stra8 gene, and knockout efficiency was evaluated in domestic chicken cells using cleavage activity of in vitro transcription of gRNA, Luciferase-SSA assay, T7 endonuclease I assay(T7E1) and TA clone sequence. In addition, the Cas9/gRNA plasmid was transfected into ESCs to confirm the function of Stra8. SSA assay results showed that luciferase activity of the vector expressing gRNA-1 and gRNA- 2 was higher than that of gRNA-3. TA clone sequencing showed that the knockdown efficiency was 25% (10/40) in DF-1 cells, the knockdown efficiency was 23% (9/40) in chicken ESCs. T7E1 assay indicated that there were cleavage activity for three individuals, and the knockdown efficiency was 12% (3/25). Cell morphology, qRT-PCR, immunostaining and FCS indicated that Cas9/gRNA not only resulted in the knockout of Stra8 gene, but also suggested that the generation of SSCs was blocked by the Stra8 gene knockdown in vitro. Taken together, our results indicate that the CRISPR/Cas9 system could mediate stable Stra8 gene knockdown in domestic chicken's cells and inhibit ECSs differentiation into SSCs.
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- 2017
- Full Text
- View/download PDF
7. Reference values of brachial-ankle pulse wave velocity according to age and blood pressure in a central Asia population.
- Author
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Gulinuer Yiming, Xianhui Zhou, Wenkui Lv, Yi Peng, Wenhui Zhang, Xinchun Cheng, Yaodong Li, Qiang Xing, Jianghua Zhang, Qina Zhou, Ling Zhang, Yanmei Lu, Hongli Wang, and Baopeng Tang
- Subjects
Medicine ,Science - Abstract
Brachial-ankle pulse wave velocity (baPWV), a direct measure of aortic stiffness, has increasingly become an important assessment for cardiovascular risk. The present study established the reference and normal values of baPWV in a Central Asia population in Xinjiang, China.We recruited participants from a central Asia population in Xinjiang, China. We performed multiple regression analysis to investigate the determinants of baPWV. The median and 10th-90th percentiles were calculated to establish the reference and normal values based on these categories.In total, 5,757 Han participants aged 15-88 years were included in the present study. Spearman correlation analysis showed that age (r = 0.587, p < 0.001) and mean blood pressure (MBP, r = 0.599, p
- Published
- 2017
- Full Text
- View/download PDF
8. The Induction Effect of Am80 and TSA on ESC Differentiation via Regulation of Stra8 in Chicken.
- Author
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Yani Zhang, Qisheng Zuo, Zhiyong Liu, Dong Li, Beibei Tang, Tian-Rong Xiao, Chao Lian, Yingjie Wang, Kai Jin, Yilin Wang, Wenhui Zhang, and Bichun Li
- Subjects
Medicine ,Science - Abstract
Stra8 encodes stimulated by retinoic acid gene 8, a protein that is important for initiation of meiosis in mammals and birds. This study was aimed at identifying the active control area of chicken STRA8 gene core promoter, to screen optimum inducers of the STRA8 gene, thus to enhance the differentiation of embryonic stem cells (ESCs) into spermatogonial stem cells. Fragments of chicken STRA8 gene promoter were cloned into fluorescent reporter plasmids and transfected into DF-1 cells. Then Dual-Luciferase® Reporter Assay System was used to identify the activity of the STRA8 gene under different inducers. Our studies showed that the promoter fragment -1055 bp to +54 bp of Suqin chicken Stra8 revealed the strongest activity. The dual-luciferase® reporter showed that Tamibarotene (Am80) and TrichostatinA (TSA) could significantly enhance STRA8 transcription. The in vitro inductive culture of chicken ESCs demonstrated that spermatogonial stem cells (SSC)-like cells appeared and Integrinβ1 protein was expressed on day 10, indicating that Am80 and TSA can promote ESCs differentiation into SSCs via regulation of Stra8.
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- 2015
- Full Text
- View/download PDF
9. Long noncoding RNA expression profiles of lung adenocarcinoma ascertained by microarray analysis.
- Author
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Gang Xu, Jie Chen, Qinshi Pan, Keta Huang, Jingye Pan, Wenhui Zhang, Jian Chen, Fangyou Yu, Tieli Zhou, and Yumin Wang
- Subjects
Medicine ,Science - Abstract
BACKGROUND:Long noncoding RNAs (lncRNAs) have been shown to be involved in the development and progression of lung cancer. However, the roles of lncRNAs in lung cancer are not well understood. METHODOLOGY/PRINCIPAL FINDINGS:We used a high-throughput microarray to compare the lncRNA and messenger RNA (mRNA) expression profiles in lung adenocarcinoma and normal tissue (NT) samples. Several candidate adenocarcinoma-associated lncRNAs were verified by real-time quantitative reverse transcription polymerase chain reaction (PCR) analysis. Using abundant and varied probes, we were able to assess 30,586 lncRNAs and 26,109 mRNAs in our microarray. We found that 2,420 lncRNAs and 1,109 mRNAs were differentially expressed (≥2-fold change) in lung adenocarcinoma samples and NT, indicating that many lncRNAs were significantly upregulated or downregulated in lung adenocarcinoma. We also found, via quantitative PCR, that 19 lncRNAs were aberrantly expressed in lung adenocarcinoma compared with matched histologically normal lung tissues. Among these, LOC100132354 and RPLP0P2 were the most aberrantly expressed lncRNAs, as estimated by quantitative PCR in 100 pairs of lung adenocarcinoma and NT samples. CONCLUSIONS/SIGNIFICANCE:Our study ascertained the expression patterns of lncRNAs in lung adenocarcinoma by microarray. The results revealed that many lncRNAs were differentially expressed in lung adenocarcinoma tissues and NT, suggesting that they may play a key role in tumor development.
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- 2014
- Full Text
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10. Potentiated interaction between ineffective doses of budesonide and formoterol to control the inhaled cadmium-induced up-regulation of metalloproteinases and acute pulmonary inflammation in rats.
- Author
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Wenhui Zhang, Jianming Zhi, Yongyao Cui, Fan Zhang, Adélite Habyarimana, Carole Cambier, and Pascal Gustin
- Subjects
Medicine ,Science - Abstract
The anti-inflammatory properties of glucocorticoids are well known but their protective effects exerted with a low potency against heavy metals-induced pulmonary inflammation remain unclear. In this study, a model of acute pulmonary inflammation induced by a single inhalation of cadmium in male Sprague-Dawley rats was used to investigate whether formoterol can improve the anti-inflammatory effects of budesonide. The cadmium-related inflammatory responses, including matrix metalloproteinase-9 (MMP-9) activity, were evaluated. Compared to the values obtained in rats exposed to cadmium, pretreatment of inhaled budesonide (0.5 mg/15 ml) elicited a significant decrease in total cell and neutrophil counts in bronchoalveolar lavage fluid (BALF) associated with a significant reduction of MMP-9 activity which was highly correlated with the number of inflammatory cells in BALF. Additionally, cadmium-induced lung injuries characterized by inflammatory cell infiltration within alveoli and the interstitium were attenuated by the pre-treatment of budesonide. Though the low concentration of budesonide (0.25 mg/15 ml) exerted a very limited inhibitory effects in the present rat model, its combination with an inefficient concentration of formoterol (0.5 mg/30 ml) showed an enhanced inhibitory effect on neutrophil and total cell counts as well as on the histological lung injuries associated with a potentiation of inhibition on the MMP-9 activity. In conclusion, high concentration of budesonide alone could partially protect the lungs against cadmium exposure induced-acute neutrophilic pulmonary inflammation via the inhibition of MMP-9 activity. The combination with formoterol could enhance the protective effects of both drugs, suggesting a new therapeutic strategy for the treatment of heavy metals-induced lung diseases.
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- 2014
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11. Maternal treatment with agonistic autoantibodies against type-1 angiotensin II receptor in late pregnancy increases apoptosis of myocardial cells and myocardial susceptibility to ischemia-reperfusion injury in offspring rats.
- Author
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Zhu Jin, Wenhui Zhang, Hailiang Yang, Xiaofang Wang, Yanqian Zheng, Qiaoyan Zhang, and Jianming Zhi
- Subjects
Medicine ,Science - Abstract
Epidemiological studies have demonstrated that offspring born to mothers preeclampsia (PE) are at increased risk for developing cardiovascular diseases after birth, but the underlying mechanism is unknown. Angiotensin II receptor type 1 autoantibody (AT1-AA), an agonist acting via activation of the AT1 receptor, is believed to be involved in the pathogenesis of both PE and fetal growth restriction. The aim of the present study was to confirm the hypothesis that prenatal AT1-AA exposure increases the heart susceptibility to ischemia/reperfusion injury (IRI) in the offspring in an AT1-AA-induced animal model of PE, and determine whether or not the increase of maternal AT1-AA level is a factor contributing to sustained abnormalities of the heart structure during infancy. The hearts of 45-day-old offspring rats were studied using Langendorff preparation to determine the susceptibility of the heart to IRI. The results showed that the body weight of the maternal rats was not significantly different between the study and control groups, but the body weight of their offspring in AT1-AA group was decreased slightly at day 21 of gestational age, and at day 3 after birth. Although the heart weight index was not significantly affected at all ages examined, AT1-AA significantly increased the size of myocardial cells of the left ventricle (LV) at the age of 45 days. AT1-AA gained access to fetal circulation via the placenta and induced apoptosis of fetal myocardial cells. AT1-AA also significantly delayed recovery from IRI and affected the LV function of 45-day-old offspring. This was associated with a significant increase in IRI-induced LV myocardial infarct size. These results suggest that AT1-AA induced abnormal apoptosis of fetal myocardial cells during the fetal period and increased the cardiac susceptibility to IRI in adult offspring.
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- 2013
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12. Characterization of Enterococcus faecalis phage IME-EF1 and its endolysin.
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Wenhui Zhang, Zhiqiang Mi, Xiuyun Yin, Hang Fan, Xiaoping An, Zhiyi Zhang, Jiankui Chen, and Yigang Tong
- Subjects
Medicine ,Science - Abstract
Enterococcus faecalis is increasingly becoming an important nosocomial infection opportunistic pathogen. E. faecalis can easily obtain drug resistance, making it difficult to be controlled in clinical settings. Using bacteriophage as an alternative treatment to drug-resistant bacteria has been revitalized recently, especially for fighting drug-resistant bacteria. In this research, an E. faecalis bacteriophage named IME-EF1 was isolated from hospital sewage. Whole genomic sequence analysis demonstrated that the isolated IME-EF1 belong to the Siphoviridae family, and has a linear double-stranded DNA genome consisting of 57,081 nucleotides. The IME-EF1 genome has a 40.04% G+C content and contains 98 putative coding sequences. In addition, IME-EF1 has an isometric head with a width of 35 nm to 60 nm and length of 75 nm to 90 nm, as well as morphology resembling a tadpole. IME-EF1 can adsorb to its host cells within 9 min, with an absorbance rate more than 99% and a latent period time of 25 min. The endolysin of IME-EF1 contains a CHAP domain in its N-terminal and has a wider bactericidal spectrum than its parental bacteriophage, including 2 strains of vancomycin-resistant E. faecalis. When administrated intraperitoneally, one dose of IME-EF1 or its endolysin can reduce bacterial count in the blood and protected the mice from a lethal challenge of E. faecalis, with a survival rate of 60% or 80%, respectively. Although bacteriophage could rescue mice from bacterial challenge, to the best of our knowledge, this study further supports the potential function of bacteriophage in dealing with E. faecalis infection in vivo. The results also indicated that the newly isolated bacteriophage IME-EF1 enriched the arsenal library of lytic E. faecalis bacteriophages and presented another choice for phage therapy in the future.
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- 2013
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13. Antibodies against AT1 receptors are associated with vascular endothelial and smooth muscle function impairment: protective effects of hydroxysafflor yellow A.
- Author
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Zhu Jin, Wenhui Zhang, Weiran Chai, Yanqian Zheng, and Jianming Zhi
- Subjects
Medicine ,Science - Abstract
Ample evidence has shown that autoantibodies against AT1 receptors (AT1-AA) are closely associated with human cardiovascular disease. The aim of this study was to investigate mechanisms underlying AT1-AA-induced vascular structural and functional impairments in the formation of hypertension, and explore ways for preventive treatment. We used synthetic peptide corresponding to the sequence of the second extracellular loop of the AT1 receptor (165-191) to immunize rats and establish an active immunization model. Part of the model received preventive therapy by losartan (20 mg/kg/day) and hyroxysafflor yellow A (HSYA) (10 mg/kg/day). The result show that systolic blood pressure (SBP) and heart rate (HR) of immunized rats was significantly higher, and closely correlated with the plasma AT1-Ab titer. The systolic response of thoracic aortic was increased, but diastolic effects were attenuated markedly. Histological observation showed that the thoracic aortic endothelium of the immunized rats became thinner or ruptured, inflammatory cell infiltration, medial smooth muscle cell proliferation and migration, the vascular wall became thicker. There was no significant difference in serum antibody titer between losartan and HSYA groups and the immunized group. The vascular structure and function were reversed, and plasma biochemical parameters were also improved significantly in the two treatment groups. These results suggest that AT1-Ab could induce injury to vascular endothelial cells, and proliferation of smooth muscle cells. These changes were involved in the formation of hypertension. Treatment with AT1 receptor antagonists and anti oxidative therapy could block the pathogenic effect of AT1-Ab on vascular endothelial and smooth muscle cells.
- Published
- 2013
- Full Text
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14. CRISPR/Cas9 mediated chicken Stra8 gene knockout and inhibition of male germ cell differentiation
- Author
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Dong Li, Man Wang, Ji Yanqin, Chen Zhang, Qisheng Zuo, Jing Jin, Wenhui Zhang, Fei Wang, Lu Zhenyu, Yingjie Wang, Bichun Li, and Yani Zhang
- Subjects
0301 basic medicine ,Male ,Cellular differentiation ,lcsh:Medicine ,Chicken Cells ,Artificial Gene Amplification and Extension ,Cell morphology ,Polymerase Chain Reaction ,Synthetic Genome Editing ,Biochemistry ,Poultry ,Genome Engineering ,Animals, Genetically Modified ,Gene Knockout Techniques ,Animal Cells ,Gamefowl ,Cloning, Molecular ,lcsh:Science ,Gene knockdown ,Multidisciplinary ,Crispr ,Cell Differentiation ,Transfection ,Enzymes ,Vertebrates ,Engineering and Technology ,Synthetic Biology ,Cellular Types ,Oxidoreductases ,Luciferase ,RNA, Guide, Kinetoplastida ,Research Article ,Biotechnology ,Transgene ,Genetic Vectors ,Bioengineering ,Biology ,Research and Analysis Methods ,Birds ,Gene Knockout ,03 medical and health sciences ,Animals ,Molecular Biology Techniques ,Molecular Biology ,Gene knockout ,Embryonic Stem Cells ,Adaptor Proteins, Signal Transducing ,030102 biochemistry & molecular biology ,lcsh:R ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,Synthetic Genomics ,Molecular biology ,Spermatogonia ,030104 developmental biology ,Germ Cells ,Fowl ,Synthetic Bioengineering ,Amniotes ,Artificial Genetic Recombination ,Enzymology ,lcsh:Q ,CRISPR-Cas Systems ,Chickens ,Developmental Biology - Abstract
An efficient genome editing approach had been established to construct the stable transgenic cell lines in the domestic chicken (Gallus gallus domesticus) at present. Our objectives were to investigate gene function in the differentiation process of chicken embryonic stem cells (ESCs) into spermatogonial stem cells(SSCs). Three guides RNA (gRNAs) were designed to knockout the Stra8 gene, and knockout efficiency was evaluated in domestic chicken cells using cleavage activity of in vitro transcription of gRNA, Luciferase-SSA assay, T7 endonuclease I assay(T7E1) and TA clone sequence. In addition, the Cas9/gRNA plasmid was transfected into ESCs to confirm the function of Stra8. SSA assay results showed that luciferase activity of the vector expressing gRNA-1 and gRNA- 2 was higher than that of gRNA-3. TA clone sequencing showed that the knockdown efficiency was 25% (10/40) in DF-1 cells, the knockdown efficiency was 23% (9/40) in chicken ESCs. T7E1 assay indicated that there were cleavage activity for three individuals, and the knockdown efficiency was 12% (3/25). Cell morphology, qRT-PCR, immunostaining and FCS indicated that Cas9/gRNA not only resulted in the knockout of Stra8 gene, but also suggested that the generation of SSCs was blocked by the Stra8 gene knockdown in vitro. Taken together, our results indicate that the CRISPR/Cas9 system could mediate stable Stra8 gene knockdown in domestic chicken's cells and inhibit ECSs differentiation into SSCs.
- Published
- 2016
15. Evaluating the influential priority of the factors on insurance loss of public transit
- Author
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Ruimin Ke, Yongmin Su, Xinqiang Chen, and Wenhui Zhang
- Subjects
Economics ,Social Sciences ,lcsh:Medicine ,Transportation ,02 engineering and technology ,Interval (mathematics) ,Grey relational analysis ,Geographical locations ,Mathematical and Statistical Techniques ,Number Theory ,Statistics ,0202 electrical engineering, electronic engineering, information engineering ,lcsh:Science ,Multidisciplinary ,Applied Mathematics ,Simulation and Modeling ,05 social sciences ,Public sector ,Accidents, Traffic ,Transportation Infrastructure ,Public transport ,Physical Sciences ,Value (economics) ,Engineering and Technology ,020201 artificial intelligence & image processing ,Management Engineering ,Algorithms ,Statistics (Mathematics) ,Research Article ,Washington ,Posterior probability ,Research and Analysis Methods ,Disease cluster ,Civil Engineering ,Insurance ,Naive Bayes classifier ,0502 economics and business ,Statistical Methods ,Risk Management ,050210 logistics & transportation ,Public Sector ,business.industry ,lcsh:R ,Probability Theory ,United States ,Roads ,North America ,lcsh:Q ,People and places ,business ,Mathematics ,Finance ,Generalized Linear Model - Abstract
Understanding correlation between influential factors and insurance losses is beneficial for insurers to accurately price and modify the bonus-malus system. Although there have been a certain number of achievements in insurance losses and claims modeling, limited efforts focus on exploring the relative role of accidents characteristics in insurance losses. The primary objective of this study is to evaluate the influential priority of transit accidents attributes, such as the time, location and type of accidents. Based on the dataset from Washington State Transit Insurance Pool (WSTIP) in USA, we implement several key algorithms to achieve the objectives. First, K-means algorithm contributes to cluster the insurance loss data into 6 intervals; second, Grey Relational Analysis (GCA) model is applied to calculate grey relational grades of the influential factors in each interval; in addition, we implement Naive Bayes model to compute the posterior probability of factors values falling in each interval. The results show that the time, location and type of accidents significantly influence the insurance loss in the first five intervals, but their grey relational grades show no significantly difference. In the last interval which represents the highest insurance loss, the grey relational grade of the time is significant higher than that of the location and type of accidents. For each value of the time and location, the insurance loss most likely falls in the first and second intervals which refers to the lower loss. However, for accidents between buses and non-motorized road users, the probability of insurance loss falling in the interval 6 tends to be highest.
- Published
- 2018
16. The Induction Effect of Am80 and TSA on ESC Differentiation via Regulation of Stra8 in Chicken
- Author
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Yingjie Wang, Bichun Li, Chao Lian, Yani Zhang, Dong Li, Wenhui Zhang, Zhiyong Liu, Qisheng Zuo, Beibei Tang, Kai Jin, Tianrong Xiao, and Yilin Wang
- Subjects
Transcriptional Activation ,Tetrahydronaphthalenes ,Cellular differentiation ,Science ,Retinoic acid ,Chick Embryo ,Biology ,Hydroxamic Acids ,Benzoates ,Avian Proteins ,Tissue Culture Techniques ,chemistry.chemical_compound ,Animals ,Promoter Regions, Genetic ,Gene ,Embryonic Stem Cells ,STRA8 Gene ,Adaptor Proteins, Signal Transducing ,Reporter gene ,Multidisciplinary ,Promoter ,Cell Differentiation ,Transfection ,Embryonic stem cell ,Molecular biology ,Histone Deacetylase Inhibitors ,chemistry ,embryonic structures ,Medicine ,Chickens ,Research Article - Abstract
Stra8 encodes stimulated by retinoic acid gene 8, a protein that is important for initiation of meiosis in mammals and birds. This study was aimed at identifying the active control area of chicken STRA8 gene core promoter, to screen optimum inducers of the STRA8 gene, thus to enhance the differentiation of embryonic stem cells (ESCs) into spermatogonial stem cells. Fragments of chicken STRA8 gene promoter were cloned into fluorescent reporter plasmids and transfected into DF-1 cells. Then Dual-Luciferase® Reporter Assay System was used to identify the activity of the STRA8 gene under different inducers. Our studies showed that the promoter fragment -1055 bp to +54 bp of Suqin chicken Stra8 revealed the strongest activity. The dual-luciferase® reporter showed that Tamibarotene (Am80) and TrichostatinA (TSA) could significantly enhance STRA8 transcription. The in vitro inductive culture of chicken ESCs demonstrated that spermatogonial stem cells (SSC)-like cells appeared and Integrinβ1 protein was expressed on day 10, indicating that Am80 and TSA can promote ESCs differentiation into SSCs via regulation of Stra8.
- Published
- 2015
17. Reference values of brachial-ankle pulse wave velocity according to age and blood pressure in a central Asia population
- Author
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Yaodong Li, Yi Peng, Qina Zhou, Xinchun Cheng, Qiang Xing, Wenkui Lv, Gulinuer Yiming, Xianhui Zhou, Hongli Wang, Wenhui Zhang, Baopeng Tang, Yanmei Lu, Jianghua Zhang, and Ling Zhang
- Subjects
Male ,Percentile ,Brachial Artery ,lcsh:Medicine ,Blood Pressure ,030204 cardiovascular system & hematology ,Vascular Medicine ,Biochemistry ,Stiffness ,Geographical Locations ,Endocrinology ,0302 clinical medicine ,Medicine and Health Sciences ,Medicine ,030212 general & internal medicine ,lcsh:Science ,Pulse wave velocity ,Aged, 80 and over ,education.field_of_study ,Multidisciplinary ,Age Factors ,Regression analysis ,Middle Aged ,Lipids ,Cholesterol ,Cardiovascular Diseases ,Physical Sciences ,Hypertension ,Regression Analysis ,Female ,Research Article ,Adult ,China ,Asia ,Adolescent ,Endocrine Disorders ,Lipoproteins ,Materials Science ,Material Properties ,Population ,Pulse Wave Analysis ,Young Adult ,03 medical and health sciences ,Linear regression ,Diabetes Mellitus ,Humans ,Mechanical Properties ,education ,Aged ,business.industry ,lcsh:R ,Biology and Life Sciences ,Proteins ,Blood pressure ,Mean blood pressure ,Dyslipidemia ,Regional Blood Flow ,Metabolic Disorders ,People and Places ,lcsh:Q ,Ankle ,business ,Body mass index ,Demography - Abstract
Background Brachial-ankle pulse wave velocity (baPWV), a direct measure of aortic stiffness, has increasingly become an important assessment for cardiovascular risk. The present study established the reference and normal values of baPWV in a Central Asia population in Xinjiang, China. Methods We recruited participants from a central Asia population in Xinjiang, China. We performed multiple regression analysis to investigate the determinants of baPWV. The median and 10th-90th percentiles were calculated to establish the reference and normal values based on these categories. Results In total, 5,757 Han participants aged 15–88 years were included in the present study. Spearman correlation analysis showed that age (r = 0.587, p < 0.001) and mean blood pressure (MBP, r = 0.599, p
- Published
- 2017
18. Potentiated Interaction between Ineffective Doses of Budesonide and Formoterol to Control the Inhaled Cadmium-Induced Up-Regulation of Metalloproteinases and Acute Pulmonary Inflammation in Rats
- Author
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Pascal Gustin, Fan Zhang, Jianming Zhi, Adélite Habyarimana, Wenhui Zhang, Yong-Yao Cui, and Carole Cambier
- Subjects
Male ,Budesonide ,Pulmonology ,Neutrophils ,Physiology ,Anti-Inflammatory Agents ,lcsh:Medicine ,Pharmacology ,Rats, Sprague-Dawley ,Leukocyte Count ,Formoterol Fumarate ,Medicine and Health Sciences ,Drug Interactions ,lcsh:Science ,Lung ,Multidisciplinary ,medicine.diagnostic_test ,Inhalation ,Respiration ,Clinical Pharmacology ,respiratory system ,Up-Regulation ,medicine.anatomical_structure ,Matrix Metalloproteinase 9 ,Ethanolamines ,Acute Disease ,Cytokines ,Chemokines ,medicine.symptom ,Bronchoalveolar Lavage Fluid ,Cadmium ,Research Article ,medicine.drug ,Drug Research and Development ,Substance-Related Disorders ,Chronic Obstructive Pulmonary Disease ,Inflammation ,Environmental and Occupational Lung Diseases ,Administration, Inhalation ,Mental Health and Psychiatry ,medicine ,Animals ,Potency ,Respiratory Physiology ,Emphysema ,business.industry ,lcsh:R ,Biology and Life Sciences ,Smoking Related Disorders ,Pneumonia ,Rats ,Bronchoalveolar lavage ,Immunology ,lcsh:Q ,Formoterol ,Physiological Processes ,business - Abstract
The anti-inflammatory properties of glucocorticoids are well known but their protective effects exerted with a low potency against heavy metals-induced pulmonary inflammation remain unclear. In this study, a model of acute pulmonary inflammation induced by a single inhalation of cadmium in male Sprague-Dawley rats was used to investigate whether formoterol can improve the anti-inflammatory effects of budesonide. The cadmium-related inflammatory responses, including matrix metalloproteinase-9 (MMP-9) activity, were evaluated. Compared to the values obtained in rats exposed to cadmium, pretreatment of inhaled budesonide (0.5 mg/15 ml) elicited a significant decrease in total cell and neutrophil counts in bronchoalveolar lavage fluid (BALF) associated with a significant reduction of MMP-9 activity which was highly correlated with the number of inflammatory cells in BALF. Additionally, cadmium-induced lung injuries characterized by inflammatory cell infiltration within alveoli and the interstitium were attenuated by the pre-treatment of budesonide. Though the low concentration of budesonide (0.25 mg/15 ml) exerted a very limited inhibitory effects in the present rat model, its combination with an inefficient concentration of formoterol (0.5 mg/30 ml) showed an enhanced inhibitory effect on neutrophil and total cell counts as well as on the histological lung injuries associated with a potentiation of inhibition on the MMP-9 activity. In conclusion, high concentration of budesonide alone could partially protect the lungs against cadmium exposure induced-acute neutrophilic pulmonary inflammation via the inhibition of MMP-9 activity. The combination with formoterol could enhance the protective effects of both drugs, suggesting a new therapeutic strategy for the treatment of heavy metals-induced lung diseases.
- Published
- 2014
19. Characterization of Enterococcus faecalis Phage IME-EF1 and Its Endolysin
- Author
-
Xiuyun Yin, Zhiyi Zhang, Zhiqiang Mi, Hang Fan, Wenhui Zhang, Xiaoping An, Jiankui Chen, and Yigang Tong
- Subjects
Phage therapy ,medicine.medical_treatment ,CHAP domain ,Lysin ,lcsh:Medicine ,Genome, Viral ,Biology ,Enterococcus faecalis ,Microbiology ,Bacteriophage ,Siphoviridae ,Mice ,Bacteriolysis ,Endopeptidases ,Gene Order ,medicine ,Animals ,Bacteriophages ,lcsh:Science ,Gram-Positive Bacterial Infections ,Phylogeny ,Multidisciplinary ,lcsh:R ,Molecular Sequence Annotation ,Sequence Analysis, DNA ,biology.organism_classification ,Lytic cycle ,lcsh:Q ,Female ,Bacteria ,Research Article - Abstract
Enterococcus faecalis is increasingly becoming an important nosocomial infection opportunistic pathogen. E. faecalis can easily obtain drug resistance, making it difficult to be controlled in clinical settings. Using bacteriophage as an alternative treatment to drug-resistant bacteria has been revitalized recently, especially for fighting drug-resistant bacteria. In this research, an E. faecalis bacteriophage named IME-EF1 was isolated from hospital sewage. Whole genomic sequence analysis demonstrated that the isolated IME-EF1 belong to the Siphoviridae family, and has a linear double-stranded DNA genome consisting of 57,081 nucleotides. The IME-EF1 genome has a 40.04% G+C content and contains 98 putative coding sequences. In addition, IME-EF1 has an isometric head with a width of 35 nm to 60 nm and length of 75 nm to 90 nm, as well as morphology resembling a tadpole. IME-EF1 can adsorb to its host cells within 9 min, with an absorbance rate more than 99% and a latent period time of 25 min. The endolysin of IME-EF1 contains a CHAP domain in its N-terminal and has a wider bactericidal spectrum than its parental bacteriophage, including 2 strains of vancomycin-resistant E. faecalis. When administrated intraperitoneally, one dose of IME-EF1 or its endolysin can reduce bacterial count in the blood and protected the mice from a lethal challenge of E. faecalis, with a survival rate of 60% or 80%, respectively. Although bacteriophage could rescue mice from bacterial challenge, to the best of our knowledge, this study further supports the potential function of bacteriophage in dealing with E. faecalis infection in vivo. The results also indicated that the newly isolated bacteriophage IME-EF1 enriched the arsenal library of lytic E. faecalis bacteriophages and presented another choice for phage therapy in the future.
- Published
- 2013
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