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Your search keyword '"Zhou, Wenbin"' showing total 13 results
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13 results on '"Zhou, Wenbin"'

2. Arabidopsis TRM5 encodes a nuclear-localised bifunctional tRNA guanine and inosine-N1-methyltransferase that is important for growth

Author

Guo, Qianqian, primary, Ng, Pei Qin, additional, Shi, Shanshan, additional, Fan, Diwen, additional, Li, Jun, additional, Zhao, Jing, additional, Wang, Hua, additional, David, Rakesh, additional, Mittal, Parul, additional, Do, Trung, additional, Bock, Ralph, additional, Zhao, Ming, additional, Zhou, Wenbin, additional, and Searle, Iain, additional

Published
2019
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6. A One-Step, Triplex, Real-Time RT-PCR Assay for the Simultaneous Detection of Enterovirus 71, Coxsackie A16 and Pan-Enterovirus in a Single Tube

Author

Ningshao Xia, Shengxiang Ge, Zhou Wenbin, Shuizhen He, Jin Wang, Shiyin Zhang, and Qiang Yan

Subjects
Serotype, Viral Diseases, Applied Microbiology, lcsh:Medicine, medicine.disease_cause, Biochemistry, Pediatrics, law.invention, law, Emerging Viral Diseases, Medicine and Health Sciences, Enterovirus 71, Public and Occupational Health, lcsh:Science, Pathogen, Polymerase chain reaction, Enterovirus, Multidisciplinary, biology, Foot-and-mouth disease, Reverse Transcriptase Polymerase Chain Reaction, Child Health, Clinical Laboratory Sciences, Infectious Diseases, Real-time polymerase chain reaction, Molecular Diagnostic Techniques, Research Article, Biotechnology, China, Real-Time Polymerase Chain Reaction, Microbiology, Virus, stomatognathic system, Diagnostic Medicine, Virology, medicine, Humans, DNA Primers, business.industry, lcsh:R, Biology and Life Sciences, biology.organism_classification, medicine.disease, Enterovirus A, Human, Viral Disease Diagnosis, Enterovirus Infection, lcsh:Q, Hand, Foot and Mouth Disease, business
Abstract

The recent, ongoing epidemic of hand, foot, and mouth disease (HFMD), which is caused by enterovirus infection, has affected millions of children and resulted in thousands of deaths in China. Enterovirus 71 (EV71) and coxsackie A16 (CA16) are the two major distinct pathogens for HFMD. However, EV71 is more commonly associated with neurologic complications and even fatalities. Therefore, simultaneously detecting and differentiating EV71 and CA16 specifically from other enteroviruses for diagnosing HFMD is important. Here, we developed a one-step, triplex, real-time RT-PCR assay for the simultaneous detection of EV71, CA16, and pan-enterovirus (EVs) in a single tube with an internal amplification control. The detection results for the serially diluted viruses indicate that the lower limit of detection for this assay is 0.001-0.04 TCID50/ml, 0.02 TCID50/ml, and 0.001 TCID50/ml for EVs, EV71, and CA16, respectively. After evaluating known HFMD virus stocks of 17 strains of 16 different serotypes, this assay showed a favorable detection spectrum and no obvious cross-reactivity. The results for 141 clinical throat swabs from HFMD-suspected patients demonstrated sensitivities of 98.4%, 98.7%, and 100% for EVs, EV71, and CA16, respectively, and 100% specificity for each virus. The application of this one-step, triplex, real-time RT-PCR assay in clinical units will contribute to HFMD surveillance and help to identify causative pathogen in patients with suspected HFMD.

Published
2014

9. Low Serum Creatine Kinase Levels in Breast Cancer Patients: A Case-Control Study

Author

Pan, Hong, primary, Xia, Kai, additional, Zhou, Wenbin, additional, Xue, Jinqiu, additional, Liang, Xiuqing, additional, Cheng, Lin, additional, Wu, Naping, additional, Liang, Mengdi, additional, Wu, Dan, additional, Ling, Lijun, additional, Ding, Qiang, additional, Chen, Lin, additional, Zha, Xiaoming, additional, Liu, Xiaoan, additional, and Wang, Shui, additional

Published
2013
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10. A Novel Finding of Sentinel Lymphatic Channels in Early Stage Breast Cancer Patients: Which May Influence Detection Rate and False-Negative Rate of Sentinel Lymph Node Biopsy

Author

Wang, Minghai, primary, Zhou, Wenbin, additional, Zhao, Yingchun, additional, Xia, Tiansong, additional, Zha, Xiaoming, additional, Ding, Qiang, additional, Liu, Xiaoan, additional, Zhao, Yi, additional, Ling, Lijun, additional, Chen, Lin, additional, and Wang, Shui, additional

Published
2012
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13. Comparison of Ablation Zones among Different Tissues Using 2450-MHz Cooled-Shaft Microwave Antenna: Results in Ex Vivo Porcine Models.

Author

Zhou, Wenbin, Liang, Mengdi, Pan, Hong, Liu, Xiaoan, Jiang, Yanni, Wang, Yufeng, Ling, Lijun, Ding, Qiang, and Wang, Shui

Subjects
*TUMOR treatment, *ABLATION techniques, *MICROWAVE antennas, *ADIPOSE tissues, *COMPARATIVE studies, *CLINICAL trials
Abstract

Background:For complete tumor ablation in different tissues, it is necessary to investigate the exact coagulation zone of microwave ablation in different tissues. The aim of this study was to compare the extent of microwave ablation zone in muscle, liver and adipose tissue in ex vivo porcine models and assess the shape of microwave coagulation zone among these tissues. Materials and Methods:Microwave ablations were performed in ex vivo porcine muscle, liver and adipose tissue using 2450-MHz cooled-shaft microwave antenna. The content of water, fat and protein in these three tissues was determined. Two power increments (40 and 80 W) and five time increments (1, 3, 5, 7, and 10 minutes) were used in this study. Diameters and shapes of the ablation zones were assessed on gross specimens. Results:The average percentages of water, fat and protein in these three tissues were significantly different (P < 0.001), respectively. The long-axis and short-axis diameters among these three tissues at each time-power combination were not significantly different (P > 0.05). The coagulation zones were all elliptical in muscle, liver and adipose tissue. When microwave ablation was performed in the tissue containing both muscle and adipose tissue, the coagulation zone was also elliptical. Regardless of the output power, the ellipticity index (EI) value of 1 minute treatment duration was higher than that of 10 minutes treatment duration (P < 0.05). Furthermore, the EI value did not decrease significantly when the treatment duration was more than 5 minutes (P > 0.05). Conclusion:The extent of microwave ablation zones was not significantly different among completely different tissues. Microwave ablations with ≥ 5 minutes time duration can induce coagulation zones with clinical desirable shape. Future clinical studies are still required to determine the role of microwave ablation in different tissues. [ABSTRACT FROM AUTHOR]

Published
2013
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