7 results on '"Yuxuan Song"'
Search Results
2. miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression.
- Author
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Lei Zhang, Xiaorui Liu, Junze Liu, Zhanqin Zhou, Yuxuan Song, Binyun Cao, and Xiaopeng An
- Subjects
Medicine ,Science - Abstract
Increasing evidence has shown that miRNAs play important roles in endometrium development during the menstrual cycle in humans and many other animals. Our previous data indicated that miR-182 levels increase 15.55-fold and pleiotrophin (PTN) levels decrease 20.97-fold in the receptive endometrium (RE, D15) compared with the pre-receptive endometrium (PE, D5) in dairy goats. The present study shows that miR-182 is widely expressed in different tissues of dairy goats and that its expression levels are regulated by E2 and P4 in endometrial epithelium cells (EECs). We confirmed that PTN is a target of miR-182 and that miR-182 regulates the protein levels of AKT, Bcl-2, FAS, MAPK, Caspase-3 and SP1 in EECs. Furthermore, miR-182 up-regulates or maintains the expression levels of osteopontin (OPN), cyclooxygenase-2 (COX-2) and prolactin receptor (PRLR) in EECs, suggesting that miR-182 is an important regulatory factor in the construction of endometrial receptivity in dairy goats. In conclusion, miR-182 participates in the development of endometrial receptivity by down-regulating PTN and affecting the expression of select apoptosis-related genes and increasing or maintaining the expression levels of OPN, COX-2 and PRLR in the EECs of dairy goats.
- Published
- 2017
- Full Text
- View/download PDF
3. Two Mutations in the Caprine MTHFR 3'UTR Regulated by MicroRNAs Are Associated with Milk Production Traits.
- Author
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Jinxing Hou, Xiaopeng An, Yuxuan Song, Teyang Gao, Yingnan Lei, and Binyun Cao
- Subjects
Medicine ,Science - Abstract
5,10-Methylenetetrahydrofolate reductase (MTHFR) plays a central role in folate metabolism by irreversibly converting 5,10-methylenetetrahydrofolate to 5-methylenetetrahydrofolate, a predominant circulating form of folate. Folate is reportedly important for milk protein synthesis, and MTHFR may be a key regulatory point of folate metabolism for milk protein synthesis in mammary epithelial cells. Prior to this study, polymorphisms of the MTHFR gene were not associated with milk production traits from a breeding perspective. Single nucleotide polymorphisms (SNPs) at microRNA (miRNA) binding sites (miR-SNPs) can affect gene expression. This study aimed to identify the effects of miR-SNPs (g.2244A>G and g.2264A>G) in the caprine MTHFR 3' UTR on the milk production traits of dairy goats.Guanzhong dairy (GD, n = 325) goats were used to detect SNPs in the caprine MTHFR 3' UTR by DNA sequencing. Two novel SNPs (g.2244A>G and g.2264A>G) were identified in the said region. The homozygous haplotype A-G of the SNPs g.2244A>G and g.2264A>G was significantly associated with milk yield and milk protein levels in GD goats (P < 0.05). Functional assays indicated that the MTHFR 2244 A → G substitution could increase the binding activity of hsa-miR-1266 with the MTHFR 3' UTR. The MTHFR 2264 A → G substitution could decrease the binding activity of hsa-miR-616 with the MTHFR 3' UTR. In addition, we observed a significant increase in the MTHFR mRNA levels of homozygous haplotype A-G carriers relative to those of homozygous haplotype G-A carriers. These results indicated that both SNPs altered the MTHFR mRNA levels. These altered levels of MTHFR mRNA may account for the association of SNPs with milk production traits.This study is the first to report that the g.2244A>G and g.2264A>G polymorphisms were associated with milk production traits in GD goats. Further investigations should explore the underlying miRNA-mediated mechanisms that are modified by the g.2244A>G and g.2264A>G SNPs. The current study evaluated these SNPs as potential genetic markers in goats, with potential applications in breeding programs.
- Published
- 2015
- Full Text
- View/download PDF
4. Identification and profiling of microRNAs in goat endometrium during embryo implantation.
- Author
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Yuxuan Song, Xiaopeng An, Lei Zhang, Mingzhe Fu, Jiayin Peng, Peng Han, Jingxing Hou, Zhanqin Zhou, and Bingyun Cao
- Subjects
Medicine ,Science - Abstract
BACKGROUND:MicroRNAs (miRNAs) are short, highly conserved small noncoding RNAs that had fundamental roles in post-transcriptional gene expression, and they are crucial for proper control of biological processes and known to participate in embryo implantation. However, miRNA expression profiles in the pre-receptive and receptive phases of the goat endometrium during embryo implantation are unknown. RESULTS:A total of 1,069 and 847 miRNAs were expressed in receptive (R) and pre-receptive (P) goat endometrium, and 632 miRNAs were co-expressed in both phases. We identified 545 (50.98%) known miRNAs in the R library and 522 (61.63%) in the P library. There were 110 up-expressed miRNAs and 33 down-expressed miRNAs in receptive endometrium compared with the pre-receptive endometrium meeting the criteria of P-values< 0.05. Moreover, GO and KEGG analysis of the target genes of the differentially expressed miRNAs revealed some candidate miRNAs, genes and pathways that may involve in the formation of the receptive endometrium. Based on stem-loop RT-qPCR, 15 miRNAs were detected and the results suggested that the majority of the miRNA expression data measured by Solexa deep sequencing could represent actual miRNA expression levels. CONCLUSIONS:Our data revealed the first miRNA profile related to the biology of the goat receptive endometrium during embryo implantation, and the results suggested that a subset of miRNAs might play important roles in the formation of endometrial receptivity. Thus, elucidating the physiological roles of endometrial miRNAs will help us better understand the genetic control of embryo implantation in goats.
- Published
- 2015
- Full Text
- View/download PDF
5. Chi-miR-4110 promotes granulosa cell apoptosis by targeting Sma- and Mad-related protein 2 (Smad2) in the caprine ovary
- Author
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Yuxuan Song, Guang Li, Shan Wang, Yue Zhang, Kaiwen Chen, Binyun Cao, Yueyu Bai, Jinxing Hou, Xiaopeng An, Xinyan Zhao, Haidong Ma, and Kexin Gao
- Subjects
0301 basic medicine ,lcsh:Medicine ,Gene Expression ,Apoptosis ,Smad2 Protein ,Biochemistry ,0302 clinical medicine ,Medicine and Health Sciences ,lcsh:Science ,3' Untranslated Regions ,Regulation of gene expression ,Mammals ,030219 obstetrics & reproductive medicine ,Multidisciplinary ,Cell Death ,Chemistry ,Follicular atresia ,Goats ,Messenger RNA ,Transfection ,Ruminants ,Enzymes ,Nucleic acids ,Ovaries ,Cell Processes ,Vertebrates ,Female ,Anatomy ,Oxidoreductases ,Luciferase ,Research Article ,03 medical and health sciences ,microRNA ,DNA-binding proteins ,Genetics ,Animals ,Non-coding RNA ,Cell Proliferation ,Granulosa Cells ,Cell growth ,Three prime untranslated region ,lcsh:R ,Ovary ,Reproductive System ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,Molecular biology ,Gene regulation ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,Amniotes ,Enzymology ,RNA ,lcsh:Q - Abstract
Follicular atresia mainly results from the apoptosis of granulosa cells (GCs). Whilst our previous investigations examined the role of chi-miR-4110 in regulating ovarian function, the present study detected the role of chi-miR-4110 in GC development. We transfected caprine GCs cultured in vitro with chi-miR-4110 mimics. Results revealed that chi-miR-4110 decreased mRNA and protein levels of Smad2 by targeting its 3'-untranslated region (3'UTR). FoxC1 and Sp1 mRNA and protein levels markedly increased, whereas those of bHLHe22 significantly decreased (P
- Published
- 2017
6. Two Mutations in the Caprine MTHFR 3'UTR Regulated by MicroRNAs Are Associated with Milk Production Traits
- Author
-
Binyun Cao, Yingnan Lei, Jinxing Hou, Teyang Gao, Yuxuan Song, and Xiaopeng An
- Subjects
Untranslated region ,Genotype ,DNA Mutational Analysis ,Quantitative Trait Loci ,lcsh:Medicine ,Single-nucleotide polymorphism ,Biology ,Polymorphism, Single Nucleotide ,Quantitative Trait, Heritable ,Polymorphism (computer science) ,Animals ,Allele ,lcsh:Science ,3' Untranslated Regions ,Alleles ,Genetic Association Studies ,Methylenetetrahydrofolate Reductase (NADPH2) ,Genetics ,Multidisciplinary ,Three prime untranslated region ,Goats ,Haplotype ,lcsh:R ,Molecular biology ,digestive system diseases ,MicroRNAs ,Milk ,Haplotypes ,Methylenetetrahydrofolate reductase ,Mutation ,biology.protein ,RNA Interference ,lcsh:Q ,Research Article - Abstract
Background 5,10-Methylenetetrahydrofolate reductase (MTHFR) plays a central role in folate metabolism by irreversibly converting 5,10-methylenetetrahydrofolate to 5-methylenetetrahydrofolate, a predominant circulating form of folate. Folate is reportedly important for milk protein synthesis, and MTHFR may be a key regulatory point of folate metabolism for milk protein synthesis in mammary epithelial cells. Prior to this study, polymorphisms of the MTHFR gene were not associated with milk production traits from a breeding perspective. Single nucleotide polymorphisms (SNPs) at microRNA (miRNA) binding sites (miR-SNPs) can affect gene expression. This study aimed to identify the effects of miR-SNPs (g.2244A>G and g.2264A>G) in the caprine MTHFR 3' UTR on the milk production traits of dairy goats. Results Guanzhong dairy (GD, n = 325) goats were used to detect SNPs in the caprine MTHFR 3' UTR by DNA sequencing. Two novel SNPs (g.2244A>G and g.2264A>G) were identified in the said region. The homozygous haplotype A-G of the SNPs g.2244A>G and g.2264A>G was significantly associated with milk yield and milk protein levels in GD goats (P < 0.05). Functional assays indicated that the MTHFR 2244 A → G substitution could increase the binding activity of hsa-miR-1266 with the MTHFR 3' UTR. The MTHFR 2264 A → G substitution could decrease the binding activity of hsa-miR-616 with the MTHFR 3' UTR. In addition, we observed a significant increase in the MTHFR mRNA levels of homozygous haplotype A-G carriers relative to those of homozygous haplotype G-A carriers. These results indicated that both SNPs altered the MTHFR mRNA levels. These altered levels of MTHFR mRNA may account for the association of SNPs with milk production traits. Conclusions This study is the first to report that the g.2244A>G and g.2264A>G polymorphisms were associated with milk production traits in GD goats. Further investigations should explore the underlying miRNA-mediated mechanisms that are modified by the g.2244A>G and g.2264A>G SNPs. The current study evaluated these SNPs as potential genetic markers in goats, with potential applications in breeding programs.
- Published
- 2015
7. miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression
- Author
-
Junze Liu, Lei Zhang, Zhanqin Zhou, Xiaopeng An, Xiaorui Liu, Binyun Cao, and Yuxuan Song
- Subjects
0301 basic medicine ,Embryology ,lcsh:Medicine ,Gene Expression ,Apoptosis ,Endometrium ,Pleiotrophin ,Epithelium ,Gene expression ,Medicine and Health Sciences ,Cell Cycle and Cell Division ,Osteopontin ,lcsh:Science ,3' Untranslated Regions ,Cells, Cultured ,Progesterone ,Mammals ,Multidisciplinary ,Cell Death ,Estradiol ,biology ,Goats ,Ruminants ,medicine.anatomical_structure ,Cell Processes ,Vertebrates ,Cytokines ,Female ,RNA Interference ,Anatomy ,Research Article ,medicine.medical_specialty ,Down-Regulation ,Estrous Cycle ,03 medical and health sciences ,Downregulation and upregulation ,Internal medicine ,microRNA ,Genetics ,medicine ,Animals ,Cell Cycle Inhibitors ,Protein kinase B ,Cell Proliferation ,Binding Sites ,Base Sequence ,Prolactin receptor ,lcsh:R ,Uterus ,Embryos ,Organisms ,Reproductive System ,Biology and Life Sciences ,Endothelial Cells ,Cell Biology ,MicroRNAs ,Biological Tissue ,030104 developmental biology ,Endocrinology ,Amniotes ,biology.protein ,lcsh:Q ,Carrier Proteins ,Proto-Oncogene Proteins c-akt ,Developmental Biology - Abstract
Increasing evidence has shown that miRNAs play important roles in endometrium development during the menstrual cycle in humans and many other animals. Our previous data indicated that miR-182 levels increase 15.55-fold and pleiotrophin (PTN) levels decrease 20.97-fold in the receptive endometrium (RE, D15) compared with the pre-receptive endometrium (PE, D5) in dairy goats. The present study shows that miR-182 is widely expressed in different tissues of dairy goats and that its expression levels are regulated by E2 and P4 in endometrial epithelium cells (EECs). We confirmed that PTN is a target of miR-182 and that miR-182 regulates the protein levels of AKT, Bcl-2, FAS, MAPK, Caspase-3 and SP1 in EECs. Furthermore, miR-182 up-regulates or maintains the expression levels of osteopontin (OPN), cyclooxygenase-2 (COX-2) and prolactin receptor (PRLR) in EECs, suggesting that miR-182 is an important regulatory factor in the construction of endometrial receptivity in dairy goats. In conclusion, miR-182 participates in the development of endometrial receptivity by down-regulating PTN and affecting the expression of select apoptosis-related genes and increasing or maintaining the expression levels of OPN, COX-2 and PRLR in the EECs of dairy goats.
- Published
- 2017
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