118 results on '"Yue Yang"'
Search Results
2. Prevalence of targeted therapy-related genetic variations in NSCLC and their relationship with clinicopathological characteristics
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Fanghua Li, Peng Ye, Peiling Cai, Dandan Dong, Yihao Zhang, Yue Yang, and Xingwang Sun
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Medicine ,Science - Abstract
Background Non-small cell lung cancer (NSCLC) is the most common cancer type in China. Targeted therapies have been used to treat NSCLC for two decades, which is only suitable for a subgroup of patients with specific genetic variations. The aim of this study was to investigate the prevalence of genetic variations leading to sensitivity or resistance to targeted therapies in NSCLC, and their relationship with clinicopathological characteristics of the patients. Methods Tumor samples were collected from 404 patients who were diagnosed to have NSCLC and underwent surgery, transthoracic biopsy, bronchoscopy biopsy, or pleural aspiration in Sichuan Provincial People’s Hospital from January 2019 to March 2020. Commercial amplification-refractory mutation system kits were used to detect targeted therapy-related genetic variations in those tumor samples. The prevalence of genetic variations and their relationship with patient clinicopathological characteristics were analyzed using statistical software, followed by subgroup analysis. Results In all, 50.7% of the NSCLC patients had sensitive genetic variations to anti-EGFR therapies, and 4.9% of those patients had co-existing resistant genetic variations. Fusions in ALK, ROS1, or RET were found in 7.7% of the patients, including 2 patients with co-existing EGFR exon 19 deletion or L858R. EGFR exon 19 deletion and L858R were more common in female patients and adenocarcinoma. Further subgroup analysis confirmed the observation in female patients in adenocarcinoma subgroup, and in adenocarcinoma in male patients. In addition, smokers were more likely to have squamous cell carcinoma and KRAS mutation and less likely to have EGFR L858R, which were also confirmed after standardization of gender except KRAS mutations. Conclusion Nearly half of the NSCLC patients were eligible for anti-EGFR treatments. In NSCLC, female gender and adenocarcinoma may indicate higher chance of EGFR exon 19 deletion or L858R, and smoking history may indicate squamous cell carcinoma and EGFR L858R.
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- 2022
3. Propofol prevents human umbilical vein endothelial cell injury from Ang II-induced apoptosis by activating the ACE2-(1-7)-Mas axis and eNOS phosphorylation.
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Liangqing Zhang, Jingjing Wang, Jiuqing Liang, Du Feng, Fan Deng, Yue Yang, Yue Lu, and Zhe Hu
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Medicine ,Science - Abstract
Angiotensin II (AngII), a vasoactive peptide that elevates arterial blood pressure and results in hypertension, has been reported to directly induce vascular endothelial cell apoptosis. Recent work has demonstrated that propofol pre-treatment attenuates angiotensin II-induced apoptosis in human coronary artery endothelial cells. However, the underlying mechanism remains largely unknown. Here, we investigated human umbilical vein endothelial cells (HUVECs) subjected to angiotensin II-induced apoptosis in the presence or absence of propofol treatment and found that angiotensin II-induced apoptosis was attenuated by propofol in a dose-dependent manner. Furthermore, ELISA assays demonstrated that the ratio of angiotensin (1-7) (Ang (1-7)) to Ang II was increased after propofol treatment. We examined the expression of ACE2, Ang (1-7) and Mas and found that the ACE2-Ang (1-7)-Mas axis was up-regulated by propofol, while ACE2 overexpression increased phosphorylated endothelial nitric oxide synthase (phosphorylated eNOS) expression and siACE2 resulted in the repression of endothelial nitric oxide synthase (eNOS) phosphorylation. In conclusion, our study revealed that propofol can inhibit endothelial cell apoptosis induced by Ang II by activating the ACE2-Ang (1-7)-Mas axis and further up-regulating the expression and phosphorylation of eNOS.
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- 2018
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4. Modeling three-dimensional invasive solid tumor growth in heterogeneous microenvironment under chemotherapy.
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Hang Xie, Yang Jiao, Qihui Fan, Miaomiao Hai, Jiaen Yang, Zhijian Hu, Yue Yang, Jianwei Shuai, Guo Chen, Ruchuan Liu, and Liyu Liu
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Medicine ,Science - Abstract
A systematic understanding of the evolution and growth dynamics of invasive solid tumors in response to different chemotherapy strategies is crucial for the development of individually optimized oncotherapy. Here, we develop a hybrid three-dimensional (3D) computational model that integrates pharmacokinetic model, continuum diffusion-reaction model and discrete cell automaton model to investigate 3D invasive solid tumor growth in heterogeneous microenvironment under chemotherapy. Specifically, we consider the effects of heterogeneous environment on drug diffusion, tumor growth, invasion and the drug-tumor interaction on individual cell level. We employ the hybrid model to investigate the evolution and growth dynamics of avascular invasive solid tumors under different chemotherapy strategies. Our simulations indicate that constant dosing is generally more effective in suppressing primary tumor growth than periodic dosing, due to the resulting continuous high drug concentration. In highly heterogeneous microenvironment, the malignancy of the tumor is significantly enhanced, leading to inefficiency of chemotherapies. The effects of geometrically-confined microenvironment and non-uniform drug dosing are also investigated. Our computational model, when supplemented with sufficient clinical data, could eventually lead to the development of efficient in silico tools for prognosis and treatment strategy optimization.
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- 2018
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5. Genetic polymorphisms and plasma levels of BCL11A contribute to the development of laryngeal squamous cell carcinoma.
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Jian Zhou, Yue Yang, Duo Zhang, Liang Zhou, Lei Tao, and Li-Ming Lu
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Medicine ,Science - Abstract
OBJECTIVE:We investigated the association between B-cell lymphoma/leukaemia 11A (BCL11A) rs11886868 and rs4671393 polymorphism, plasma BCL11A concentration, and the hazard of developing laryngeal squamous cell carcinoma (LSCC). PARTICIPANTS AND METHOD:In this research, 330 LSCC patients, 310 healthy controls, and 155 vocal leukoplakia patients were genotyped for the BCL11A (rs11886868 C/T and rs4671393 A/G) genotypes by pyrosequencing; the BCL11A concentration was measured using ELISA. RESULTS:LSCC Patients had a notably higher occurrence of CT at rs11886868 (OR = 2.64, P = 0.025) than the control group; they also had higher GG at rs4671393 (OR = 2.53, P = 0.018). Advanced (III and IV) stage LSCC patients had a notably greater frequency of CT at rs11886868 than those with initial (I and II) stage LSCC (OR = 2.71, P = 0.044 vs. OR = 2.58, P = 0.051). Additionally, there was a 1.59 fold increase in susceptibility for initial stage LSCC related to the G allele (AG/GG) at rs4671393 (P = 0.005); while for patients of advanced stage LSCC the OR was 1.73 (P = 0.002). Moreover, the OR of lymph node metastasis patients at rs4671393 G alleles was 2.41 (P < 0.01); it was 1.38 (P = 0.035) in patients without lymph metastasis. Patients with high incidences of the rs4671393 variation genotype had high plasma BCL11A levels. CONCLUSIONS:BCL11A rs11886868 and rs4671393 genotype variations and correspondingly high BCL11A plasma levels are related to LSCC, besides, differences in plasma levels and genotype distribution may be related to lymph node metastasis status and the stage of LSCC.
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- 2017
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6. The effects of different frequency treadmill exercise on lipoxin A4 and articular cartilage degeneration in an experimental model of monosodium iodoacetate-induced osteoarthritis in rats.
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Yue Yang, Yang Wang, Yawei Kong, Xiaoning Zhang, and Lunhao Bai
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Medicine ,Science - Abstract
The aim was to investigate the effects of different frequencies treadmill exercise with total exercise time being constancy on articular cartilage, lipoxin A4 (LXA4) and the NF-κB pathway in rat model of monosodium iodoacetate-induced osteoarthritis (OA). Fifty male Sprague-Dawley rats were randomly divided into five groups (n = 10): controls (CG), knee OA model (OAG), OA + treadmill exercise once daily (OAE1), OA + treadmill exercise twice daily, rest interval between exercise>4h (OAE2) and OA + treadmill exercise three times daily, rest interval between exercise>4h (OAE3). Rats were evaluated after completing the treadmill exercise program (speed, 18 m/min; total exercise time 60 min/day; 5 days/week for 8 weeks). Interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and LXA4 in serum and intra-articular lavage fluid were measured by ELISA. Changes in articular cartilage were evaluated by histology, immunohistochemistry, western blotting and quantitative real-time-PCR. LXA4 in the serum and intra-articular lavage fluid increased in all OAE groups, and histological evaluation indicated that the OAE3 group had the best treatment response. The expression of COL2A1 and IκB-β in articular cartilage increased in all OAE groups vs the OAG group, whereas expression of IL-1β, TNF-α, matrix metalloproteinase (MMP)-13, and NF-κB p65 was reduced in all OAE groups compared with the OAG. Under the condition of 60 min treadmill exercise with moderate-intensity, to fulfill in three times would have better chondroprotective effects than to fulfill in two or one time on monosodium iodoacetate-induced OA in rats. And it may be worked through the anti-inflammatory activity of LXA4 and the NF-κB pathway.
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- 2017
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7. Nampt Expression Decreases Age-Related Senescence in Rat Bone Marrow Mesenchymal Stem Cells by Targeting Sirt1.
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Cao Ma, Chenchen Pi, Yue Yang, Lin Lin, Yingai Shi, Yan Li, Yulin Li, and Xu He
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Medicine ,Science - Abstract
Senescence restricts the development of applications involving mesenchymal stem cells (MSCs) in research fields, such as tissue engineering, and stem cell therapeutic strategies. Understanding the mechanisms underlying natural aging processes may contribute to the development of novel approaches to preventing age-related diseases or slowing individual aging processes. Nampt is a rate-limiting NAD biosynthetic enzyme that plays critical roles in energy metabolism, cell senescence and maintaining life spans. However, it remains unknown whether Nampt influences stem cell senescence. In this study, the function of Nampt was investigated using a rat model of natural aging. Our data show that Nampt expression was significantly lower in MSCs obtained from aged rats than in those obtained from young rats during physiological aging. Reducing the level of Nampt in aged MSCs resulted in lower intracellular concentrations of NAD+ and downregulated Sirt1 expression and activity. After the Nampt inhibitor FK866 was added, young MSCs were induced to become aged cells. The enhanced senescence was correlated with NAD+ depletion and Sirt1 activity attenuation. In addition, Nampt overexpression attenuated cell senescence in aged MSCs. Our findings provide a new explanation for the mechanisms underlying stem cell senescence and a novel target for delaying stem cell senescence and preventing and treating age-related diseases.
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- 2017
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8. Evolution of scaling behaviors embedded in sentence series from A Story of the Stone.
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Yue Yang, Changgui Gu, Qin Xiao, and Huijie Yang
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Medicine ,Science - Abstract
The novel entitled A Story of the Stone provides us precise details of life and social structure of the 18th century China. Its writing lasted a long duration of about 10 years, in which the author's habit may change significantly. It had been published anonymously up to the beginning of the 20th century, which left a mystery of the author's attribution. In the present work we focus our attention on scaling behavior embedded in the sentence series from this novel, hope to find how the ideas are organized from single sentences to the whole text. Especially we are interested in the evolution of scale invariance to monitor the changes of the author's language habit and to find some clues on the author's attribution. The sentence series are separated into a total of 69 non-overlapping segments with a length of 500 sentences each. The correlation dependent balanced estimation of diffusion entropy (cBEDE) is employed to evaluate the scaling behaviors embedded in the short segments. It is found that the total, the part attributed currently to Xueqin Cao (X-part), and the other part attributed to E Gao (E-part), display scale invariance in a large scale up to 103 sentences, while their scaling exponents are almost identical. All the segments behave scale invariant in considerable wide scales, most of which reach one third of the length. In the curve of scaling exponent versus segment number, the X-part has rich patterns with averagely larger values, while the E-part has a U-shape with a significant low bottom. This finding is a new clue to support the attribution of the E-part to E Gao.
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- 2017
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9. A Meta-Analysis of Antiviral Therapy for Hepatitis B Virus-Associated Membranous Nephropathy.
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Yue Yang, Ye-Ping Ma, Da-Peng Chen, Li Zhuo, and Wen-Ge Li
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Medicine ,Science - Abstract
Hepatitis B virus-associated membranous nephropathy (HBV-MN) is the most common renal extra-hepatic manifestation in patients with chronic HBV infection. In September 2015, we searched the MEDLINE, EMBASE, and CENTRAL databases, and the reference lists of retrieved articles, to identify relevant studies. Descriptions of antiviral drugs used to treat HBV-MN were included in our review. Two authors independently screened all relevant articles, extracted data, and assessed the risk of bias. Nine hundred and fifty-four papers have been considered after electronic and manual searching, only five relevant studies were identified. Complete remission (OR = 26.87, 95% CI: 8.06 to 89.52), total remission (OR = 10.31, 95% CI: 3.59 to 29.63) of proteinuria and HBeAg clearance (OR = 20.91, 95% CI: 6.90 to 63.39) increased significantly after antiviral therapy. No significant differences were seen between interferon and nucleoside analog treatments. Our study found that antiviral therapy was an effective treatment in HBV-MN patients; interferon and nucleoside analogs were equally effective at causing proteinuria remission and HBeAg clearance.
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- 2016
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10. Correction: Effect of Pore Size and Porosity on the Biomechanical Properties and Cytocompatibility of Porous NiTi Alloys.
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Yu-Tao Jian, Yue Yang, Tian Tian, Clark Stanford, Xin-Ping Zhang, and Ke Zhao
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Medicine ,Science - Published
- 2016
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11. ENTPD5 induces apoptosis in lung cancer cells via regulating caspase 3 expression.
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Yijun Xue, Lina Wu, Yinan Liu, Yuanyuan Ma, Lijian Zhang, Xuemei Ma, Yue Yang, and Jinfeng Chen
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Medicine ,Science - Abstract
This study is to investigate the relationship between ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5) expression and lung cancer clinicopathological factors, and the impact of ENTPD5 on lung cancer cell functions. Lung cancer specimens and matched adjacent normal tissues were obtained from patients without any preoperative radiotherapy or chemotherapy. Knockdown of ETNPD5 expression led to significantly decreased lung cancer cell growth rate, markedly increased apoptosis and the ability to repair, and significantly reduced invasion. Gene chip tests showed that knockdown of ENTPD5 expression caused more Caspase expression. Quantitative real-time polymerase chain reaction showed that the Caspase 3 expression was significantly increased after the knockdown of ENTPD5. In addition, immunohistochemistry showed that the tumor growth marker, proliferating cell nuclear antigen, was significantly reduced in the knockdown model. Tumorigenicity assay and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay showed that the apoptosis of lung cancer cells was increased in the knockdown model. Our results suggest that ENTPD5 affects lung cancer apoptosis via Caspase 3 pathway, and can be potentially used to monitor prognosis or to guide appropriate therapeutic regimens.
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- 2015
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12. EMX2 Is a Predictive Marker for Adjuvant Chemotherapy in Lung Squamous Cell Carcinomas.
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Dongsheng Yue, Hui Li, Juanjuan Che, Yi Zhang, Bhairavi Tolani, Minli Mo, Hua Zhang, Qingfeng Zheng, Yue Yang, Runfen Cheng, Joy Q Jin, Thomas W Luh, Cathryn Yang, Hsin-Hui K Tseng, Etienne Giroux-Leprieur, Gavitt A Woodard, Xishan Hao, Changli Wang, David M Jablons, and Biao He
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Medicine ,Science - Abstract
Squamous cell carcinomas (SCC) account for approximately 30% of non-small cell lung cancer (NSCLC). Current staging methods do not adequately predict outcome for this disease. EMX2 is a homeo-domain containing transcription factor known to regulate a key developmental pathway. This study assessed the significance of EMX2 as a prognostic and predictive marker for resectable lung SCC.Two independent cohorts of patients with lung SCC undergoing surgical resection were studied. EMX2 protein expression was examined by immunohistochemistry, Western blot, or immunofluorescence. EMX2 expression levels in tissue specimens were scored and correlated with patient outcomes. Chemo-sensitivity of lung SCC cell lines stably transfected with EMX2 shRNAs to cisplatin, carboplatin, and docetaxel was examined in vitro.EMX2 expression was down-regulated in lung SCC tissue samples compared to their matched adjacent normal tissues. Positive EMX2 expression was significantly associated with improved overall survival in stage I lung SCC patients, and in stage II/IIIA lung SCC patients receiving adjuvant chemotherapy. EMX2 expression was also associated with expression of EMT markers in both lung SCC cell lines and tissue samples. Knock-down of EMX2 expression in lung SCC cells promoted chemo-resistance and cell migration.EMX2 expression is down-regulated in lung SCC and its down-regulation is associated with chemo-resistance in lung SCC cells, possibly through regulation of Epithelial-to-Mesenchymal Transition (EMT). EMX2 may serve as a novel prognostic marker for stage I lung SCC patients and a prediction marker for stage II/IIIA lung SCC patients receiving adjuvant chemotherapy.
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- 2015
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13. Grape Seed Proanthocyanidin Extract Ameliorates Diabetic Bladder Dysfunction via the Activation of the Nrf2 Pathway.
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Shouzhen Chen, Yaofeng Zhu, Zhifeng Liu, Zhaoyun Gao, Baoying Li, Dongqing Zhang, Zhaocun Zhang, Xuewen Jiang, Zhengfang Liu, Lingquan Meng, Yue Yang, and Benkang Shi
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Medicine ,Science - Abstract
Diabetes Mellitus (DM)-induced bladder dysfunction is predominantly due to the long-term oxidative stress caused by hyperglycemia. Grape seed proanthocyanidin extract (GSPE) has been reported to possess a broad spectrum of pharmacological and therapeutic properties against oxidative stress. However, its protective effects against diabetic bladder dysfunction have not been clarified. This study focuses on the effects of GSPE on bladder dysfunction in diabetic rats induced by streptozotocin. After 8 weeks of GSPE administration, the bladder function of the diabetic rats was improved significantly, as indicated by both urodynamics analysis and histopathological manifestation. Moreover, the disordered activities of antioxidant enzymes (SOD and GSH-Px) and abnormal oxidative stress levels were partly reversed by treatment with GSPE. Furthermore, the level of apoptosis in the bladder caused by DM was decreased following the administration of GSPE according to the Terminal Deoxynucleotidyl Transferase (TdT)-mediated dUTP Nick-End Labeling (TUNEL) assay. Additionally, GSPE affected the expression of apoptosis-related proteins such as Bax, Bcl-2 and cleaved caspase-3. Furthermore, GSPE showed neuroprotective effects on the bladder of diabetic rats, as shown by the increased expression of nerve growth factor (NGF) and decreased expression of the precursor of nerve growth factor (proNGF). GSPE also activated nuclear erythroid2-related factor2 (Nrf2), which is a key antioxidative transcription factor, with the concomitant elevation of downstream hemeoxygenase-1 (HO-1). These findings suggested that GSPE could ameliorate diabetic bladder dysfunction and decrease the apoptosis of the bladder in diabetic rats, a finding that may be associated with its antioxidant activity and ability to activate the Nrf2 defense pathway.
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- 2015
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14. Effect of Pore Size and Porosity on the Biomechanical Properties and Cytocompatibility of Porous NiTi Alloys.
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Yu-Tao Jian, Yue Yang, Tian Tian, Clark Stanford, Xin-Ping Zhang, and Ke Zhao
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Medicine ,Science - Abstract
Five types of porous Nickel-Titanium (NiTi) alloy samples of different porosities and pore sizes were fabricated. According to compressive and fracture strengths, three groups of porous NiTi alloy samples underwent further cytocompatibility experiments. Porous NiTi alloys exhibited a lower Young's modulus (2.0 GPa ~ 0.8 GPa). Both compressive strength (108.8 MPa ~ 56.2 MPa) and fracture strength (64.6 MPa ~ 41.6 MPa) decreased gradually with increasing mean pore size (MPS). Cells grew and spread well on all porous NiTi alloy samples. Cells attached more strongly on control group and blank group than on all porous NiTi alloy samples (p < 0.05). Cell adhesion on porous NiTi alloys was correlated negatively to MPS (277.2 μm ~ 566.5 μm; p < 0.05). More cells proliferated on control group and blank group than on all porous NiTi alloy samples (p < 0.05). Cellular ALP activity on all porous NiTi alloy samples was higher than on control group and blank group (p < 0.05). The porous NiTi alloys with optimized pore size could be a potential orthopedic material.
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- 2015
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15. Altered resting-state amygdala functional connectivity after 36 hours of total sleep deprivation.
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Yongcong Shao, Yu Lei, Lubin Wang, Tianye Zhai, Xiao Jin, Wei Ni, Yue Yang, Shuwen Tan, Bo Wen, Enmao Ye, and Zheng Yang
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Medicine ,Science - Abstract
ObjectivesRecent neuroimaging studies have identified a potentially critical role of the amygdala in disrupted emotion neurocircuitry in individuals after total sleep deprivation (TSD). However, connectivity between the amygdala and cerebral cortex due to TSD remains to be elucidated. In this study, we used resting-state functional MRI (fMRI) to investigate the functional connectivity changes of the basolateral amygdala (BLA) and centromedial amygdala (CMA) in the brain after 36 h of TSD.Materials and methodsFourteen healthy adult men aged 25.9 ± 2.3 years (range, 18-28 years) were enrolled in a within-subject crossover study. Using the BLA and CMA as separate seed regions, we examined resting-state functional connectivity with fMRI during rested wakefulness (RW) and after 36 h of TSD.ResultsTSD resulted in a significant decrease in the functional connectivity between the BLA and several executive control regions (left dorsolateral prefrontal cortex [DLPFC], right dorsal anterior cingulate cortex [ACC], right inferior frontal gyrus [IFG]). Increased functional connectivity was found between the BLA and areas including the left posterior cingulate cortex/precuneus (PCC/PrCu) and right parahippocampal gyrus. With regard to CMA, increased functional connectivity was observed with the rostral anterior cingulate cortex (rACC) and right precentral gyrus.ConclusionThese findings demonstrate that disturbance in amygdala related circuits may contribute to TSD psychophysiology and suggest that functional connectivity studies of the amygdala during the resting state may be used to discern aberrant patterns of coupling within these circuits after TSD.
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- 2014
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16. Identification of gene expression changes from colitis to CRC in the mouse CAC model.
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Xin Li, Yuyan Gao, Ming Yang, Qi Zhao, Guangyu Wang, Yan Mei Yang, Yue Yang, Hui Liu, and Yanqiao Zhang
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Medicine ,Science - Abstract
A connection between colorectal carcinogenesis and inflammation is well known, but the underlying molecular mechanisms have not been elucidated. Chemically induced colitis-associated cancer (CAC) is an outstanding mouse model for studying the link between inflammation and cancer. Additionally, the CAC model is used for examining novel diagnostic, prognostic, and predictive markers for use in clinical practice. Here, a CAC model was established in less than 100 days using azoxymethane (AOM) with dextran sulfate sodium salt (DSS) in BALB/c mice. We examined the mRNA expression profiles of three groups: control untreated mice (K), DSS-induced chronic colitis mice (D), and AOM/DSS-induced CAC (AD) mice. We identified 6301 differentially expressed genes (DEGs) among the three groups, including 93 persistently upregulated genes and 139 persistently downregulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the most persistent DEGs were significantly enriched in metabolic or inflammatory components in the tumor microenvironment. Furthermore, several associated DEGs were identified as potential DEGs by protein-protein interaction (PPI) network analysis. We selected 14 key genes from the DEGs and potential DEGs for further quantitative real-time PCR (qPCR) verification. Six persistently upregulated, 3 persistently downregulated DEGs, and the other 3 genes showed results consistent with the microarray data. We demonstrated the regulation of 12 key genes specifically involved in Wnt signaling, cytokine and cytokine receptor interactions, homeostasis, and tumor-associated metabolism during colitis-associated CRC. Our results suggest that a close relationship between metabolic and inflammatory mediators of the tumor microenvironment is present in CAC.
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- 2014
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17. Lower intensified target LDL-c level of statin therapy results in a higher risk of incident diabetes: a meta-analysis.
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Rongrong Cai, Yang Yuan, Yi Zhou, Wenqing Xia, Pin Wang, Haixia Sun, Yue Yang, Rong Huang, and Shaohua Wang
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Medicine ,Science - Abstract
A recent meta-analysis has reported that intensive-dose statin drug increases the risk of incident diabetes. However, doubling of the statin dose generates only a further 6% decrease in low-density lipoprotein cholesterol (LDL-c) on average. This study aimed to determine whether statin therapy with lower intensive-target LDL-c level contributes to higher risk of new-onset diabetes.Medline, Embase, and the Cochrane Central Register of Controlled Trials were searched for randomized controlled endpoint trials of statins conducted from 1966 to 2012. We included trials with more than 1000 participants who were followed up for at least 2 years. The included trials were stratified by the target LDL-c level. I2 statistic was used to measure heterogeneity between trials. We further calculated risk estimates with random-effect meta-analysis. Meta-regression was used to identify the potential risk factors of statin-induced diabetes.Fourteen trials with a total of 95 102 non-diabetic participants were included. The risks elevated by 33% [odds ratio (OR) = 1.33; 95% confidence interval (CI) 1.14-1.56; I(2) = 7.7%] and 16% (OR = 1.16; 95% CI 1.06-1.28; I(2)= 0.0%) when the intensified target LDL-c levels were ≤ 1.8 mmol/L and 1.8-2.59 mmol/L, respectively. The risk of incident diabetes did not increase when the target LDL-c level was ≥ 2.59 mmol/L. Apart from age, female, and baseline level of total cholesterol, meta-regression analysis showed that the target and baseline levels of LDL-c and relative LDL-c reduction were predictors of statin-induced diabetes.A lower intensified target LDL-c level of statin therapy resulted in a higher risk of incident diabetes.
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- 2014
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18. Understanding a substrate's product regioselectivity in a family of enzymes: a case study of acetaminophen binding in cytochrome P450s.
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Yue Yang, Sergio E Wong, and Felice C Lightstone
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Medicine ,Science - Abstract
Product regioselectivity as influenced by molecular recognition is a key aspect of enzyme catalysis. We applied large-scale two-dimensional (2D) umbrella sampling (USP) simulations to characterize acetaminophen (APAP) binding in the active sites of the family of Cytochrome P450 (CYP) enzymes as a case study to show the different regioselectivity exhibited by a single substrate in comparative enzymes. Our results successfully explain the experimentally observed product regioselectivity for all five human CYPs included in this study, demonstrating that binding events play an important role in determining regioselectivity. In CYP2C9 and CYP3A4, weak interactions in an overall large active site cavity result in a fairly small binding free energy difference between APAP reactive binding states, consistent with experimental results that show little preference for resulting metabolites. In contrast, in CYP1A2 and CYP2E1, APAP is strongly restrained by a compact binding pocket, leading to a preferred binding conformation. The calculated binding equilibrium of APAP within the compact active site of CYP2A6 is able to predict the experimentally documented product ratios and is also applied to explain APAP regioselectivity in CYP1A2 and CYP2C9. APAP regioselectivity seems to be related to the selectivity for one binding conformation over another binding conformation as dictated by the size and shape of the active site. Additionally, unlike docking and molecular dynamics (MD), our free energy calculations successfully reproduced a unique APAP pose in CYP3A4 that had been reported experimentally, suggesting this approach is well suited to find the realistic binding pose and the lowest-energy starting structure for studying the chemical reaction step in the future.
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- 2014
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19. Elevated maspin expression is associated with better overall survival in esophageal squamous cell carcinoma (ESCC).
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Yang Wang, Shijie Sheng, Jianzhi Zhang, Sijana Dzinic, Shaolei Li, Fang Fang, Nan Wu, Qingfeng Zheng, and Yue Yang
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Medicine ,Science - Abstract
Tumor suppressor maspin is a differentially regulated gene in the progression of many types of cancer. While the biological function of maspin in blocking tumor invasion and metastasis is consistent with the loss of maspin expression at the late stage of tumor progression, the differential expression and the biological significance of maspin in early stage of tumor progression appear to be complex and remain to be elucidated. In the current study, we examined the expression of maspin in 84 esophageal squamous cell carcinoma (ESCC) cases (stages I-III) and 55 non-tumor adjacent esophageal tissue specimens by immunohistochemical (IHC) staining. The correlation of maspin with clinicopathological parameters was analyzed. Compared to normal esophageal squamous tissue where 80% (47/55) of the cases expressed maspin at a low to moderate level, all ESCC specimens (100% (84/84)) were positive for maspin expression at a moderate to high level. ESCC with low or moderate maspin expression had significantly shorter postoperative survival rates compared to those that had high maspin expression (p
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- 2013
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20. Implications of false negative and false positive diagnosis in lymph node staging of NSCLC by means of ¹⁸F-FDG PET/CT.
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Shaolei Li, Qingfeng Zheng, Yuanyuan Ma, Yuzhao Wang, Yuan Feng, Bingtian Zhao, and Yue Yang
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Medicine ,Science - Abstract
BACKGROUND:Integrated ¹⁸F-fluorodeoxyglucose positron emission tomography/computed tomography (¹⁸F-FDG PET/CT) is widely performed in hilar and mediastinal lymph node (HMLN) staging of non-small cell lung cancer (NSCLC). However, the diagnostic efficiency of PET/CT remains controversial. This retrospective study is to evaluate the accuracy of PET/CT and the characteristics of false negatives and false positives to improve specificity and sensitivity. METHODS:219 NSCLC patients with systematic lymph node dissection or sampling underwent preoperative PET/CT scan. Nodal uptake with a maximum standardized uptake value (SUV(max)) >2.5 was interpreted as PET/CT positive. The results of PET/CT were compared with the histopathological findings. The receiver operating characteristic (ROC) curve was generated to determine the diagnostic efficiency of PET/CT. Univariate and multivariate analysis were conducted to detect risk factors of false negatives and false positives. RESULTS:The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of PET/CT in detecting HMLN metastases were 74.2% (49/66), 73.2% (112/153), 54.4% (49/90), 86.8% (112/129), and 73.5% (161/219). The ROC curve had an area under curve (AUC) of 0.791 (95% CI 0.723-0.860). The incidence of false negative HMLN metastases was 13.2% (17 of 129 patients). Factors that are significantly associated with false negatives are: concurrent lung disease or diabetes (p4.0 (p=0.009). Postoperatively, 45.5% (41/90) patients were confirmed as false positive cases. The univariate analysis indicated age > 65 years old (p=0.009), well differentiation (p=0.002), and SUV(max) of primary tumor ≦4.0 (p=0.007) as risk factors for false positive uptake. CONCLUSION:The SUV(max) of HMLN is a predictor of malignancy. Lymph node staging using PET/CT is far from equal to pathological staging account of some risk factors. This study may provide some aids to pre-therapy evaluation and decision-making.
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- 2013
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21. Regional brain structural abnormality in meal-related functional dyspepsia patients: a voxel-based morphometry study.
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Fang Zeng, Wei Qin, Yue Yang, Danhua Zhang, Jixin Liu, Guangyu Zhou, Jinbo Sun, Shengfeng Lu, Yong Tang, Yuan Chen, Lei Lan, Shuguang Yu, Ying Li, Xin Gao, Qiyong Gong, Jie Tian, and Fanrong Liang
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Medicine ,Science - Abstract
BACKGROUND AND AIMS: Brain dysfunction in functional dyspepsia (FD) has been identified by multiple neuroimaging studies. This study aims to investigate the regional gray matter density (GMD) changes in meal-related FD patients and their correlations with clinical variables, and to explore the possible influence of the emotional state on FD patients's brain structures. METHODS: Fifty meal-related FD patients and forty healthy subjects (HS) were included and underwent a structural magnetic resonance imaging scan. Voxel-based morphometry analysis was employed to identify the cerebral structure alterations in meal-related FD patients. Regional GMD changes' correlations with the symptoms and their durations, respectively, have been analyzed. RESULTS: Compared to the HS, the meal-related FD patients showed a decreased GMD in the bilateral precentral gyrus, medial prefrontal cortex (MPFC), anterior cingulate cortex (ACC) and midcingulate cortex (MCC), left orbitofrontal cortex (OFC) and right insula (p50). After controlling for anxiety and depression, the meal-related FD patients showed a decreased GMD in the bilateral middle frontal gyrus, left MCC, right precentral gyrus and insula (p50). Before controlling psychological factors, the GMD decreases in the ACC were negatively associated with the symptom scores of the Nepean Dyspepsia Index (NDI) (r = -0.354, p = 0.048, Bonferroni correction) and the duration of FD (r = -0.398, p = 0.02, Bonferroni correction) respectively. CONCLUSIONS: The regional GMD of meal-related FD patients, especially in the regions of the homeostatic afferent processing network significantly differed from that of the HS, and the psychological factors might be one of the essential factors significantly affecting the regional brain structure of meal-related FD patients.
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- 2013
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22. Suppressed retinal degeneration in aged wild type and APPswe/PS1ΔE9 mice by bone marrow transplantation.
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Yue Yang, Christine Shiao, Jake Frederick Hemingway, Nikolas L Jorstad, Bryan Richard Shalloway, Rubens Chang, and C Dirk Keene
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Medicine ,Science - Abstract
Alzheimer's disease (AD) is an age-related condition characterized by accumulation of neurotoxic amyloid β peptides (Aβ) in brain and retina. Because bone marrow transplantation (BMT) results in decreased cerebral Aβ in experimental AD, we hypothesized that BMT would mitigate retinal neurotoxicity through decreased retinal Aβ. To test this, we performed BMT in APPswe/PS1ΔE9 double transgenic mice using green fluorescent protein expressing wild type (wt) mice as marrow donors. We first examined retinas from control, non-transplanted, aged AD mice and found a two-fold increase in microglia compared with wt mice, prominent inner retinal Aβ and paired helical filament-tau, and decreased retinal ganglion cell layer neurons. BMT resulted in near complete replacement of host retinal microglia with BMT-derived cells and normalized total AD retinal microglia to non-transplanted wt levels. Aβ and paired helical filament-tau were reduced (61.0% and 44.1% respectively) in BMT-recipient AD mice, which had 20.8% more retinal ganglion cell layer neurons than non-transplanted AD controls. Interestingly, aged wt BMT recipients also had significantly more neurons (25.4%) compared with non-transplanted aged wt controls. Quantitation of retinal ganglion cell layer neurons in young mice confirmed age-related retinal degeneration was mitigated by BMT. We found increased MHC class II expression in BMT-derived microglia and decreased oxidative damage in retinal ganglion cell layer neurons. Thus, BMT is neuroprotective in age-related as well as AD-related retinal degeneration, and may be a result of alterations in innate immune function and oxidative stress in BMT recipient mice.
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- 2013
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23. Reduced expression of Enac in Placenta tissues of patients with severe preeclampsia is related to compromised trophoblastic cell migration and invasion during pregnancy.
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Shan Wang, Guolin He, Yue Yang, Ying Liu, Ruiying Diao, Kai Sheng, Xinghui Liu, and Wenming Xu
- Subjects
Medicine ,Science - Abstract
The purpose of the study is to investigate the expression of epithelial sodium channel (ENaC) in normal pregnancy and severe preeclampsia placenta and to explore the underlying mechanism of the relationship between the altered ENaC expression and onset of preeclampsia. Reverse transcription polymerase chain reaction (RT-PCR) and Western blot were used to check epithelial sodium channel subunits expression in mRNA and protein level in first term and full term placental tissue. ENaCα specific RNAi were used to knockdown ENaC expression and cell invasion and migration assay were used to check whether reduced expression of ENaC can compromise trophoblast cell function. The result showed that ENaCα was highly expressed in first term placental trophoblast cells; while EnaCβ was highly expressed in full term placenta. Knockdown ENaCα expression by using small interfering RNA reduced the invasive and migration abilities of HTR-8/SVneo cell. Real time-PCR and Western blot analysis showed that the expression levels of ENaCβ were also significantly lower in severe preeclampsia compared with normal pregnancy. It is concluded that the ENaC played an important role in trophoblast cell invasion and migration. Reduced expression and activity of epithelial sodium channel in trophoblast cells may be involved in the pathogenesis of preeclampsia.
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- 2013
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24. Decreased thalamocortical functional connectivity after 36 hours of total sleep deprivation: evidence from resting state FMRI.
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Yongcong Shao, Lubin Wang, Enmao Ye, Xiao Jin, Wei Ni, Yue Yang, Bo Wen, Dewen Hu, and Zheng Yang
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Medicine ,Science - Abstract
OBJECTIVES: The thalamus and cerebral cortex are connected via topographically organized, reciprocal connections, which hold a key function in segregating internally and externally directed awareness information. Previous task-related studies have revealed altered activities of the thalamus after total sleep deprivation (TSD). However, it is still unclear how TSD impacts on the communication between the thalamus and cerebral cortex. In this study, we examined changes of thalamocortical functional connectivity after 36 hours of total sleep deprivation by using resting state function MRI (fMRI). MATERIALS AND METHODS: Fourteen healthy volunteers were recruited and performed fMRI scans before and after 36 hours of TSD. Seed-based functional connectivity analysis was employed and differences of thalamocortical functional connectivity were tested between the rested wakefulness (RW) and TSD conditions. RESULTS: We found that the right thalamus showed decreased functional connectivity with the right parahippocampal gyrus, right middle temporal gyrus and right superior frontal gyrus in the resting brain after TSD when compared with that after normal sleep. As to the left thalamus, decreased connectivity was found with the right medial frontal gyrus, bilateral middle temporal gyri and left superior frontal gyrus. CONCLUSION: These findings suggest disruptive changes of the thalamocortical functional connectivity after TSD, which may lead to the decline of the arousal level and information integration, and subsequently, influence the human cognitive functions.
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- 2013
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25. Molecular phylogeny of the butterfly genus Polytremis (Hesperiidae, Hesperiinae, Baorini) in China.
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Weibin Jiang, Jianqing Zhu, Chao Song, Xiaoyan Li, Yue Yang, and Weidong Yu
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Medicine ,Science - Abstract
BACKGROUND: The genus Polytremis, restricted to the continental part of the southeastern Palaearctic and northern Oriental regions, is one of the largest and most diverse lineages of the tribe Baorini. Previous studies on the genus were focused mainly on morphological classification and identification of new species. Due to the lack of effective and homologous traits of morphology, there were many challenges in the traditional classification. In this report, we reconstruct the phylogeny to provide a solid framework for these studies and to test the traditional limits and relationships of taxa. METHODOLOGY AND PRINCIPAL FINDINGS: We sequenced a mitochondrial and three nuclear gene fragments, coupled with an evaluation of traditional morphological characters, to determine the phylogenetic relationships for a total of 15 species representing all major species groups of the Polytremis genus in China, and to elucidate their taxonomic status. CONCLUSIONS AND SIGNIFICANCE: Analysis of mitochrondial and nuclear DNA showed considerable congruent phylogenetic signal in topology at the inter-species level. We found strong support for the monophyly of Polytremis and some clades were recognized with morphological data. Thus, the COI sequence in our study could be used as a DNA barcode to identify almost all members of the genus. However, incongruences of phylogenetic analyses occurred: in contrast to the phylogenetic trees of mitochondrial COI, it was not possible for nuclear rDNA to discriminate P. gotama from P. caerulescens, suggesting a possible recent separation of these two species. Additionally, P. theca was the only species with a greater intra-specific genetic distance compared to some inter-specific genetic distances in this study and some problems associated with the cryptic diversity of the species are discussed. The results of this study will helpful to reveal the causes of the high degree of diversity of butterflies, and possibly other groups of insects in China.
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- 2013
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26. Association analysis of IL-17A and IL-17F polymorphisms in Chinese Han women with breast cancer.
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Lihong Wang, Yongdong Jiang, Youxue Zhang, Yuwen Wang, Sunhui Huang, Zhihua Wang, Baoling Tian, Yue Yang, Wei Jiang, and Da Pang
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Medicine ,Science - Abstract
BACKGROUND: Research into the etiology of breast cancer has recently focused on the role of the immunity and inflammation. The proinflammatory cytokines IL-17A and IL-17F can mediate inflammation and cancer. To evaluate the influences of IL-17A and IL-17F gene polymorphisms on the risk of sporadic breast cancer, a case-control study was conducted in Chinese Han women. METHODOLOGY AND PRINCIPAL FINDINGS: We genotyped three single-nucleotide polymorphisms (SNPs) in IL-17A (rs2275913, rs3819025 and rs3748067) and five SNPs in IL-17F (rs7771511, rs9382084, rs12203582, rs1266828 and rs763780) to determine the haplotypes in 491 women with breast cancer and 502 healthy individuals. The genotypes were determined using the SNaPshot technique. The differences in the genotypic distribution between breast cancer patients and healthy controls were analyzed with the Chi-square test for trends. For rs2275913 in IL-17A, the frequency of the AA genotype was higher in patients than controls (P = 0.0016). The clinical features analysis demonstrated significant associations between IL-17 SNPs and tumor protein 53 (P53), progesterone receptor (PR), human epidermal growth factor receptor 2 (Her-2) and triple-negative (ER-/PR-/Her-2-) status. In addition, the haplotype analysis indicated that the frequency of the haplotype A(rs2275913)G(rs3819025)G(rs3748067), located in the IL-17A linkage disequilibrium (LD) block, was higher in patients than in controls (P = 0.0471 after correction for multiple testing). CONCLUSIONS AND SIGNIFICANCE: Our results suggested that SNPs in IL-17A but not IL-17F were associated with the risk of breast cancer. Both IL-17A and IL-17F gene polymorphisms may provide valuable information for predicting the prognosis of breast cancer in Chinese women.
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- 2012
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27. A J-like protein influences fatty acid composition of chloroplast lipids in Arabidopsis.
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Imad Ajjawi, Ardian Coku, John E Froehlich, Yue Yang, Katherine W Osteryoung, Christoph Benning, and Robert L Last
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Medicine ,Science - Abstract
A comprehensive understanding of the lipid and fatty acid metabolic machinery is needed for optimizing production of oils and fatty acids for fuel, industrial feedstocks and nutritional improvement in plants. T-DNA mutants in the poorly annotated Arabidopsis thaliana gene At1g08640 were identified as containing moderately high levels (50-100%) of 16∶1Δ7 and 18∶1Δ9 leaf fatty acids and subtle decreases (5-30%) of 16∶3 and 18∶3 (http://www.plastid.msu.edu/). TLC separation of fatty acids in the leaf polar lipids revealed that the chloroplastic galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) were the main lipid types affected by this mutation. Analysis of the inferred amino acid sequence of At1g08640 predicted the presence of a transit peptide, three transmembrane domains and an N-terminal J-like domain, and the gene was named CJD1 for Chloroplast J-like Domain 1. GFP reporter experiments and in vitro chloroplast import assays demonstrated CJD1 is a chloroplast membrane protein. Screening of an Arabidopsis cDNA library by yeast-2-hybrid (Y2H) using the J-like domain of CJD1 as bait identified a plastidial inner envelope protein (Accumulation and Replication of Chloroplasts 6, ARC6) as the primary interacting partner in the Y2H assay. ARC6 plays a central role in chloroplast division and binds CJD1 via its own J-like domain along with an adjacent conserved region whose function is not fully known. These results provide a starting point for future investigations of how mutations in CJD1 affect lipid composition.
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- 2011
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28. Identification of hub genes related to the recovery phase of irradiation injury by microarray and integrated gene network analysis.
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Jing Zhang, Yue Yang, Yin Wang, Jinyuan Zhang, Zejian Wang, Ming Yin, and Xudong Shen
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Medicine ,Science - Abstract
BACKGROUND: Irradiation commonly causes long-term bone marrow injury charactertized by defective HSC self-renewal and a decrease in HSC reserve. However, the effect of high-dose IR on global gene expression during bone marrow recovery remains unknown. METHODOLOGY: Microarray analysis was used to identify differentially expressed genes that are likely to be critical for bone marrow recovery. Multiple bioinformatics analyses were conducted to identify key hub genes, pathways and biological processes. PRINCIPAL FINDINGS: 1) We identified 1302 differentially expressed genes in murine bone marrow at 3, 7, 11 and 21 days after irradiation. Eleven of these genes are known to be HSC self-renewal associated genes, including Adipoq, Ccl3, Ccnd1, Ccnd2, Cdkn1a, Cxcl12, Junb, Pten, Tal1, Thy1 and Tnf; 2) These 1302 differentially expressed genes function in multiple biological processes of immunity, including hematopoiesis and response to stimuli, and cellular processes including cell proliferation, differentiation, adhesion and signaling; 3) Dynamic Gene Network analysis identified a subgroup of 25 core genes that participate in immune response, regulation of transcription and nucleosome assembly; 4) A comparison of our data with known irradiation-related genes extracted from literature showed 42 genes that matched the results of our microarray analysis, thus demonstrated consistency between studies; 5) Protein-protein interaction network and pathway analyses indicated several essential protein-protein interactions and signaling pathways, including focal adhesion and several immune-related signaling pathways. CONCLUSIONS: Comparisons to other gene array datasets indicate that global gene expression profiles of irradiation damaged bone marrow show significant differences between injury and recovery phases. Our data suggest that immune response (including hematopoiesis) can be considered as a critical biological process in bone marrow recovery. Several critical hub genes that are key members of significant pathways or gene networks were identified by our comprehensive analysis.
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- 2011
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29. Na+/K+-ATPase α1 identified as an abundant protein in the blood-labyrinth barrier that plays an essential role in the barrier integrity.
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Yue Yang, Min Dai, Teresa M Wilson, Irina Omelchenko, John E Klimek, Phillip A Wilmarth, Larry L David, Alfred L Nuttall, Peter G Gillespie, and Xiaorui Shi
- Subjects
Medicine ,Science - Abstract
BACKGROUND:The endothelial-blood/tissue barrier is critical for maintaining tissue homeostasis. The ear harbors a unique endothelial-blood/tissue barrier which we term "blood-labyrinth-barrier". This barrier is critical for maintaining inner ear homeostasis. Disruption of the blood-labyrinth-barrier is closely associated with a number of hearing disorders. Many proteins of the blood-brain-barrier and blood-retinal-barrier have been identified, leading to significant advances in understanding their tissue specific functions. In contrast, capillaries in the ear are small in volume and anatomically complex. This presents a challenge for protein analysis studies, which has resulted in limited knowledge of the molecular and functional components of the blood-labyrinth-barrier. In this study, we developed a novel method for isolation of the stria vascularis capillary from CBA/CaJ mouse cochlea and provided the first database of protein components in the blood-labyrinth barrier as well as evidence that the interaction of Na(+)/K(+)-ATPase α1 (ATP1A1) with protein kinase C eta (PKCη) and occludin is one of the mechanisms of loud sound-induced vascular permeability increase. METHODOLOGY/PRINCIPAL FINDINGS:Using a mass-spectrometry, shotgun-proteomics approach combined with a novel "sandwich-dissociation" method, more than 600 proteins from isolated stria vascularis capillaries were identified from adult CBA/CaJ mouse cochlea. The ion transporter ATP1A1 was the most abundant protein in the blood-labyrinth barrier. Pharmacological inhibition of ATP1A1 activity resulted in hyperphosphorylation of tight junction proteins such as occludin which increased the blood-labyrinth-barrier permeability. PKCη directly interacted with ATP1A1 and was an essential mediator of ATP1A1-initiated occludin phosphorylation. Moreover, this identified signaling pathway was involved in the breakdown of the blood-labyrinth-barrier resulting from loud sound trauma. CONCLUSIONS/SIGNIFICANCE:The results presented here provide a novel method for capillary isolation from the inner ear and the first database on protein components in the blood-labyrinth-barrier. Additionally, we found that ATP1A1 interaction with PKCη and occludin was involved in the integrity of the blood-labyrinth-barrier.
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- 2011
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30. Mutational escape in HIV-1 CTL epitopes leads to increased binding to inhibitory myelomonocytic MHC class I receptors.
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Yue Yang, Jinghe Huang, Ildiko Toth, Mathias Lichterfeld, and Xu G Yu
- Subjects
Medicine ,Science - Abstract
Escape mutations in HIV-1 cytotoxic T cell (CTL) epitopes can abrogate recognition by the TCR of HIV-1-specific CD8+ T cells, but may also change interactions with alternative MHC class I receptors. Here, we show that mutational escape in three HLA-A11-, B8- and B7- restricted immunodominant HIV-1 CTL epitopes consistently enhances binding of the respective peptide/MHC class I complex to Immunoglobulin-like transcript 4 (ILT4), an inhibitory myelomonocytic MHC class I receptor expressed on monocytes and dendritic cells. In contrast, mutational escape in an alternative immunodominant HLA-B57-restricted CTL epitope did not affect ILT4-mediated recognition by myelomonocytic cells. This suggests that in addition to abrogating recognition by HIV-1-specific CD8 T cells, mutational escape in some, but not all CTL epitopes may mediate important immunoregulatory effects by increasing binding properties to ILT4, and augmenting ILT4-mediated inhibitory effects of professional antigen-presenting cells.
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- 2010
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31. The silkworm (Bombyx mori) microRNAs and their expressions in multiple developmental stages.
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Xiaomin Yu, Qing Zhou, Sung-Chou Li, Qibin Luo, Yimei Cai, Wen-chang Lin, Huan Chen, Yue Yang, Songnian Hu, and Jun Yu
- Subjects
Medicine ,Science - Abstract
MicroRNAs (miRNAs) play crucial roles in various physiological processes through post-transcriptional regulation of gene expressions and are involved in development, metabolism, and many other important molecular mechanisms and cellular processes. The Bombyx mori genome sequence provides opportunities for a thorough survey for miRNAs as well as comparative analyses with other sequenced insect species.We identified 114 non-redundant conserved miRNAs and 148 novel putative miRNAs from the B. mori genome with an elaborate computational protocol. We also sequenced 6,720 clones from 14 developmental stage-specific small RNA libraries in which we identified 35 unique miRNAs containing 21 conserved miRNAs (including 17 predicted miRNAs) and 14 novel miRNAs (including 11 predicted novel miRNAs). Among the 114 conserved miRNAs, we found six pairs of clusters evolutionarily conserved cross insect lineages. Our observations on length heterogeneity at 5' and/or 3' ends of nine miRNAs between cloned and predicted sequences, and three mature forms deriving from the same arm of putative pre-miRNAs suggest a mechanism by which miRNAs gain new functions. Analyzing development-related miRNAs expression at 14 developmental stages based on clone-sampling and stem-loop RT PCR, we discovered an unusual abundance of 33 sequences representing 12 different miRNAs and sharply fluctuated expression of miRNAs at larva-molting stage. The potential functions of several stage-biased miRNAs were also analyzed in combination with predicted target genes and silkworm's phenotypic traits; our results indicated that miRNAs may play key regulatory roles in specific developmental stages in the silkworm, such as ecdysis.Taking a combined approach, we identified 118 conserved miRNAs and 151 novel miRNA candidates from the B. mori genome sequence. Our expression analyses by sampling miRNAs and real-time PCR over multiple developmental stages allowed us to pinpoint molting stages as hotspots of miRNA expression both in sorts and quantities. Based on the analysis of target genes, we hypothesized that miRNAs regulate development through a particular emphasis on complex stages rather than general regulatory mechanisms.
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- 2008
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32. Research on comprehensive evaluation & development of aesthetic education based on PCA and CEM model.
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Xu, Xin-Hong, Niu, Yu-Ting, Li, Zhi-Min, Xu, Yue-Yang, and Cao, Ke-Wang
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AESTHETICS education ,PRINCIPAL components analysis ,HUMANISTIC education ,CREATIVE thinking ,ASSESSMENT of education - Abstract
Aesthetic education, conveyed through public art courses, serves as a vital form of humanistic literacy education. It represents an effective approach to fostering innovative and creative thinking among college students. In order to effectively analyze the aesthetic education work of 46 universities, an aesthetic education index evaluation system is constructed, involving indicators including faculty strength, curriculum setting, teaching management, artistic practice, and teaching support. The secondary indicators corresponding to the five indicators are statistically analyzed, and a comprehensive evaluation analysis of the current development status of aesthetic education in 46 universities in Anhui Province is conducted by combining theoretical analysis with empirical analysis. Based on principal component analysis, an integrated evaluation model for the development of aesthetic education in universities in Anhui Province is further constructed. The model designed quantifies the influence weight of each aesthetic education index on the development of aesthetic education in Anhui Province, and forges a theoretical basis for determining the precursors of rapid development of aesthetic education in Anhui Province. Additionally, a novel approach is introduced to gauge the progression of aesthetic education within universities in Anhui Province, considering the dispersion of aesthetic education index data across the province. The comprehensive evaluation model for the development of aesthetic education in Anhui Province exhibits an overall declining trend. Hence, it is suggested to utilize the maximum value of the first derivative of the comprehensive evaluation model as an indicator of the imminent rapid development of aesthetic education in Anhui Province. On this basis, the probability equation of sustainable development of aesthetic education in Anhui Province is defined. Overall, the research results lay a theoretical foundation for the development of aesthetic education in Anhui Province. [ABSTRACT FROM AUTHOR]
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- 2024
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33. Targeted metabolomics reveals the association between central carbon metabolism and pulmonary nodules
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Wu, Yue-yang, primary, Shen, Wen-bin, additional, Li, Jian-wei, additional, Liu, Meng-yu, additional, Hu, Wen-lei, additional, Wang, Sheng, additional, Liu, Jian-jun, additional, Huang, Fen, additional, and Qin, Qi-rong, additional
- Published
- 2023
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34. Urban commuting dynamics in response to public transit upgrades: A big data approach
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Gao, Qi-Li, primary, Li, Qing-Quan, additional, Zhuang, Yan, additional, Yue, Yang, additional, Liu, Zhen-Zhen, additional, Li, Shui-Quan, additional, and Sui, Daniel, additional
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- 2019
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35. The effects of different frequency treadmill exercise on lipoxin A4 and articular cartilage degeneration in an experimental model of monosodium iodoacetate-induced osteoarthritis in rats
- Author
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Yawei Kong, Yang Wang, Xiaoning Zhang, Yue Yang, and Lunhao Bai
- Subjects
0301 basic medicine ,Cartilage, Articular ,Male ,Pathology ,Physical fitness ,Arthritis ,lcsh:Medicine ,Osteoarthritis ,Knee Joints ,Pathology and Laboratory Medicine ,Polymerase Chain Reaction ,Rats, Sprague-Dawley ,0302 clinical medicine ,Medicine and Health Sciences ,Public and Occupational Health ,Enzyme-Linked Immunoassays ,lcsh:Science ,Immune Response ,Musculoskeletal System ,Multidisciplinary ,NF-kappa B ,Interleukin ,Immunohistochemistry ,Sports Science ,Lipoxins ,medicine.anatomical_structure ,Connective Tissue ,Anatomy ,Signal Transduction ,Research Article ,medicine.medical_specialty ,Histology ,Blotting, Western ,Immunology ,Connective tissue ,Enzyme-Linked Immunosorbent Assay ,Iodoacetates ,Research and Analysis Methods ,03 medical and health sciences ,Signs and Symptoms ,Rheumatology ,Diagnostic Medicine ,Internal medicine ,Physical Conditioning, Animal ,medicine ,Animals ,Sports and Exercise Medicine ,Immunoassays ,Exercise ,030203 arthritis & rheumatology ,Inflammation ,business.industry ,Cartilage ,lcsh:R ,Biology and Life Sciences ,Physical Activity ,medicine.disease ,Disease Models, Animal ,Joints (Anatomy) ,030104 developmental biology ,Endocrinology ,Biological Tissue ,Physical Fitness ,Immunologic Techniques ,lcsh:Q ,Joints ,business ,Articular Cartilage - Abstract
The aim was to investigate the effects of different frequencies treadmill exercise with total exercise time being constancy on articular cartilage, lipoxin A4 (LXA4) and the NF-κB pathway in rat model of monosodium iodoacetate-induced osteoarthritis (OA). Fifty male Sprague-Dawley rats were randomly divided into five groups (n = 10): controls (CG), knee OA model (OAG), OA + treadmill exercise once daily (OAE1), OA + treadmill exercise twice daily, rest interval between exercise>4h (OAE2) and OA + treadmill exercise three times daily, rest interval between exercise>4h (OAE3). Rats were evaluated after completing the treadmill exercise program (speed, 18 m/min; total exercise time 60 min/day; 5 days/week for 8 weeks). Interleukin (IL)-1β, tumor necrosis factor (TNF)-α, and LXA4 in serum and intra-articular lavage fluid were measured by ELISA. Changes in articular cartilage were evaluated by histology, immunohistochemistry, western blotting and quantitative real-time-PCR. LXA4 in the serum and intra-articular lavage fluid increased in all OAE groups, and histological evaluation indicated that the OAE3 group had the best treatment response. The expression of COL2A1 and IκB-β in articular cartilage increased in all OAE groups vs the OAG group, whereas expression of IL-1β, TNF-α, matrix metalloproteinase (MMP)-13, and NF-κB p65 was reduced in all OAE groups compared with the OAG. Under the condition of 60 min treadmill exercise with moderate-intensity, to fulfill in three times would have better chondroprotective effects than to fulfill in two or one time on monosodium iodoacetate-induced OA in rats. And it may be worked through the anti-inflammatory activity of LXA4 and the NF-κB pathway.
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- 2017
36. Risk factors associated with sexually transmitted infections among HIV infected men who have sex with men
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Peng Peng, Xuan Zhang, Ping Ma, Bowen Zhu, Minjie Chu, Wenjie Jiang, Xiaojun Meng, Ye Wei, Xun Zhuang, Changqing Yang, Liying Jiang, Hongli Xia, Yun Xian, and Yue Yang
- Subjects
0301 basic medicine ,RNA viruses ,Marginal structural model ,lcsh:Medicine ,Drug resistance ,Logistic regression ,Pathology and Laboratory Medicine ,Men who have sex with men ,0302 clinical medicine ,Immunodeficiency Viruses ,Hiv infected ,Medicine and Health Sciences ,Medicine ,030212 general & internal medicine ,lcsh:Science ,Multidisciplinary ,Transmission (medicine) ,virus diseases ,HIV diagnosis and management ,Infectious Diseases ,Medical Microbiology ,Viral Pathogens ,Viruses ,Pathogens ,Research Article ,HIV infections ,Sexually Transmitted Diseases ,Men WHO Have Sex with Men ,Viral diseases ,Microbiology ,03 medical and health sciences ,Microbial Control ,Retroviruses ,Microbial Pathogens ,Pharmacology ,Biology and life sciences ,business.industry ,Lentivirus ,lcsh:R ,Organisms ,HIV ,Targeted interventions ,030112 virology ,Diagnostic medicine ,Increased risk ,Co-Infections ,People and Places ,HIV-1 ,Population Groupings ,lcsh:Q ,Antimicrobial Resistance ,business ,Demography ,Sexuality Groupings - Abstract
To investigate the factors associated with sexually transmitted infection and Human Immunodeficiency Virus (STI-HIV) co-infection among men who have sex with men (MSM). A total of 357 HIV-infected participants (84 STI-HIV co-infection and 273 HIV infections only) were recruited from Jiangsu, China. Logistic regression analyses were used to estimate the related factors associated with STI-HIV co-infection. Marginal structural models were adopted to estimate the effect of transmission drug resistance (TDR) on STI-HIV co-infection. For all participants, logistic regression analyses revealed that those who diagnosed with HIV-1 for longer duration (≥1.8 years) were significantly associated with reduced STI-HIV co-infection risk (OR = 0.55, 95%CI: 0.32-0.96, P = 0.036). In further stratification analysis by antiretroviral therapy (ART), individuals with longer duration showed consistent significant associations with STI-HIV co-infection risk (OR = 0.46, 95%CI: 0.26-0.83, P = 0.010) among MSM with ART-naive status. In addition, significant reduced risk for STI-HIV co-infection (OR = 0.98, 95%CI: 0.96-0.99, P = 0.010) were observed in younger (under the average age of 31.03) MSM of the same group. Interestingly, we also found TDR was significantly associated with an increased risk of STI-HIV co-infection risk (OR = 3.84, 95%CI: 1.05-14.03, P = 0.042) in ART-naive group. Our study highlights a pattern of STI-HIV co-infection among MSM in China and indicates that targeted interventions aimed at encouraging TDR monitoring in MSM with early HIV infection are warranted.
- Published
- 2017
37. Modeling three-dimensional invasive solid tumor growth in heterogeneous microenvironment under chemotherapy
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Miaomiao Hai, Jianwei Shuai, Jiaen Yang, Qihui Fan, Zhijian Hu, Liyu Liu, Hang Xie, Guo Chen, Yang Jiao, Ruchuan Liu, and Yue Yang
- Subjects
0301 basic medicine ,medicine.medical_treatment ,Cancer Treatment ,lcsh:Medicine ,0302 clinical medicine ,Neoplasms ,Medicine and Health Sciences ,Tumor Microenvironment ,Cell Cycle and Cell Division ,Tissues and Organs (q-bio.TO) ,lcsh:Science ,Solid tumor ,Drug Distribution ,Mass Diffusivity ,Multidisciplinary ,Invasive Tumors ,Pharmaceutics ,Chemistry ,Physics ,Chemical Reactions ,Primary tumor ,Oncology ,Cell Processes ,030220 oncology & carcinogenesis ,Physical Sciences ,Hybrid model ,Research Article ,Clinical Oncology ,In silico ,Antineoplastic Agents ,Cellular level ,Models, Biological ,Cancer Chemotherapy ,03 medical and health sciences ,Drug Therapy ,medicine ,Chemotherapy ,Humans ,Pharmacokinetics ,Neoplasm Invasiveness ,Tumor growth ,Quantitative Biology - Populations and Evolution ,Cell Proliferation ,Pharmacology ,Decomposition ,Chemical Physics ,lcsh:R ,Populations and Evolution (q-bio.PE) ,Cancers and Neoplasms ,Biology and Life Sciences ,Quantitative Biology - Tissues and Organs ,Cell Biology ,medicine.disease ,030104 developmental biology ,Drug concentration ,FOS: Biological sciences ,Cancer research ,lcsh:Q ,Clinical Medicine - Abstract
A systematic understanding of the evolution and growth dynamics of invasive solid tumors in response to different chemotherapy strategies is crucial for the development of individually optimized oncotherapy. Here, we develop a hybrid three-dimensional (3D) computational model that integrates pharmacokinetic model, continuum diffusion-reaction model and discrete cell automaton model to investigate 3D invasive solid tumor growth in heterogeneous microenvironment under chemotherapy. Specifically, we consider the effects of heterogeneous environment on drug diffusion, tumor growth, invasion and the drug-tumor interaction on individual cell level. We employ the hybrid model to investigate the evolution and growth dynamics of avascular invasive solid tumors under different chemotherapy strategies. Our simulations reproduce the well-established observation that constant dosing is generally more effective in suppressing primary tumor growth than periodic dosing, due to the resulting continuous high drug concentration. In highly heterogeneous microenvironment, the malignancy of the tumor is significantly enhanced, leading to inefficiency of chemotherapies. The effects of geometrically-confined microenvironment and non-uniform drug dosing are also investigated. Our computational model, when supplemented with sufficient clinical data, could eventually lead to the development of efficient in silico tools for prognosis and treatment strategy optimization., Comment: 41 pages, 8 figures
- Published
- 2018
38. Genetic polymorphisms and plasma levels of BCL11A contribute to the development of laryngeal squamous cell carcinoma
- Author
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Yue Yang, Lei Tao, Liming Lu, Duo Zhang, Jian Zhou, and Liang Zhou
- Subjects
0301 basic medicine ,Larynx ,Oncology ,Male ,Heredity ,Physiology ,lcsh:Medicine ,Metastasis ,0302 clinical medicine ,Gene Frequency ,Genotype ,Basic Cancer Research ,Medicine and Health Sciences ,lcsh:Science ,Leukoplakia ,Multidisciplinary ,Alcohol Consumption ,Nuclear Proteins ,Squamous Cell Carcinomas ,Hematology ,Middle Aged ,Body Fluids ,Genetic Mapping ,medicine.anatomical_structure ,Blood ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Female ,Lymph ,Anatomy ,Research Article ,medicine.medical_specialty ,Variant Genotypes ,Polymorphism, Single Nucleotide ,Carcinomas ,Blood Plasma ,Lymphatic System ,03 medical and health sciences ,Breast cancer ,Internal medicine ,medicine ,Genetics ,Humans ,Genetic Predisposition to Disease ,Allele ,Laryngeal Neoplasms ,Alleles ,Aged ,Nutrition ,business.industry ,lcsh:R ,Biology and Life Sciences ,Cancers and Neoplasms ,medicine.disease ,Lymphoma ,Diet ,Repressor Proteins ,030104 developmental biology ,lcsh:Q ,Lymph Nodes ,business ,Carrier Proteins - Abstract
Objective We investigated the association between B-cell lymphoma/leukaemia 11A (BCL11A) rs11886868 and rs4671393 polymorphism, plasma BCL11A concentration, and the hazard of developing laryngeal squamous cell carcinoma (LSCC). Participants and method In this research, 330 LSCC patients, 310 healthy controls, and 155 vocal leukoplakia patients were genotyped for the BCL11A (rs11886868 C/T and rs4671393 A/G) genotypes by pyrosequencing; the BCL11A concentration was measured using ELISA. Results LSCC Patients had a notably higher occurrence of CT at rs11886868 (OR = 2.64, P = 0.025) than the control group; they also had higher GG at rs4671393 (OR = 2.53, P = 0.018). Advanced (III and IV) stage LSCC patients had a notably greater frequency of CT at rs11886868 than those with initial (I and II) stage LSCC (OR = 2.71, P = 0.044 vs. OR = 2.58, P = 0.051). Additionally, there was a 1.59 fold increase in susceptibility for initial stage LSCC related to the G allele (AG/GG) at rs4671393 (P = 0.005); while for patients of advanced stage LSCC the OR was 1.73 (P = 0.002). Moreover, the OR of lymph node metastasis patients at rs4671393 G alleles was 2.41 (P < 0.01); it was 1.38 (P = 0.035) in patients without lymph metastasis. Patients with high incidences of the rs4671393 variation genotype had high plasma BCL11A levels. Conclusions BCL11A rs11886868 and rs4671393 genotype variations and correspondingly high BCL11A plasma levels are related to LSCC, besides, differences in plasma levels and genotype distribution may be related to lymph node metastasis status and the stage of LSCC.
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- 2016
39. A Meta-Analysis of Antiviral Therapy for Hepatitis B Virus-Associated Membranous Nephropathy
- Author
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Ye-ping Ma, Li Zhuo, Dapeng Chen, Wenge Li, and Yue Yang
- Subjects
030232 urology & nephrology ,Glycobiology ,lcsh:Medicine ,medicine.disease_cause ,Pathology and Laboratory Medicine ,Gastroenterology ,Biochemistry ,Glomerulonephritis, Membranous ,Database and Informatics Methods ,0302 clinical medicine ,Mathematical and Statistical Techniques ,Medicine and Health Sciences ,Medicine ,Public and Occupational Health ,Hepatitis B e Antigens ,Database Searching ,lcsh:Science ,Multidisciplinary ,Proteinuria ,Pharmaceutics ,Nucleosides ,Hepatitis B ,Medical microbiology ,Vaccination and Immunization ,Glycosylamines ,HBeAg ,Meta-analysis ,Viruses ,Physical Sciences ,030211 gastroenterology & hepatology ,medicine.symptom ,Pathogens ,Statistics (Mathematics) ,medicine.drug ,Research Article ,medicine.medical_specialty ,Hepatitis B virus ,Immunology ,Research and Analysis Methods ,Microbiology ,Antiviral Agents ,03 medical and health sciences ,Pharmacotherapy ,Signs and Symptoms ,Membranous nephropathy ,Antiviral Therapy ,Drug Therapy ,Diagnostic Medicine ,Internal medicine ,Humans ,Statistical Methods ,Nucleoside analogue ,business.industry ,lcsh:R ,Viral pathogens ,Organisms ,Biology and Life Sciences ,Proteins ,medicine.disease ,Hepatitis viruses ,Microbial pathogens ,lcsh:Q ,Interferons ,Preventive Medicine ,business ,Mathematics ,Meta-Analysis - Abstract
Hepatitis B virus-associated membranous nephropathy (HBV-MN) is the most common renal extra-hepatic manifestation in patients with chronic HBV infection. In September 2015, we searched the MEDLINE, EMBASE, and CENTRAL databases, and the reference lists of retrieved articles, to identify relevant studies. Descriptions of antiviral drugs used to treat HBV-MN were included in our review. Two authors independently screened all relevant articles, extracted data, and assessed the risk of bias. Nine hundred and fifty-four papers have been considered after electronic and manual searching, only five relevant studies were identified. Complete remission (OR = 26.87, 95% CI: 8.06 to 89.52), total remission (OR = 10.31, 95% CI: 3.59 to 29.63) of proteinuria and HBeAg clearance (OR = 20.91, 95% CI: 6.90 to 63.39) increased significantly after antiviral therapy. No significant differences were seen between interferon and nucleoside analog treatments. Our study found that antiviral therapy was an effective treatment in HBV-MN patients; interferon and nucleoside analogs were equally effective at causing proteinuria remission and HBeAg clearance.
- Published
- 2016
40. Subsoiling practices change root distribution and increase post-anthesis dry matter accumulation and yield in summer maize
- Author
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Sun, Xuefang, primary, Ding, Zaisong, additional, Wang, Xinbing, additional, Hou, Haipeng, additional, Zhou, Baoyuan, additional, Yue, Yang, additional, Ma, Wei, additional, Ge, Junzhu, additional, Wang, Zhimin, additional, and Zhao, Ming, additional
- Published
- 2017
- Full Text
- View/download PDF
41. Grape Seed Proanthocyanidin Extract Ameliorates Diabetic Bladder Dysfunction via the Activation of the Nrf2 Pathway
- Author
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Zhengfang Liu, Xuewen Jiang, Yue Yang, Lingquan Meng, Yaofeng Zhu, Baoying Li, Dongqing Zhang, Zhaoyun Gao, Benkang Shi, Zhifeng Liu, Shouzhen Chen, and Zhaocun Zhang
- Subjects
medicine.medical_specialty ,food.ingredient ,NF-E2-Related Factor 2 ,Urinary Bladder ,lcsh:Medicine ,Apoptosis ,medicine.disease_cause ,Neuroprotection ,Diabetes Mellitus, Experimental ,Diabetes Complications ,food ,Internal medicine ,medicine ,Animals ,Proanthocyanidins ,Rats, Wistar ,lcsh:Science ,Multidisciplinary ,Urinary bladder ,TUNEL assay ,Grape Seed Extract ,business.industry ,lcsh:R ,Streptozotocin ,Rats ,Oxidative Stress ,Endocrinology ,medicine.anatomical_structure ,Terminal deoxynucleotidyl transferase ,Grape seed extract ,lcsh:Q ,Female ,business ,Oxidative stress ,medicine.drug ,Research Article ,Signal Transduction - Abstract
Diabetes Mellitus (DM)-induced bladder dysfunction is predominantly due to the long-term oxidative stress caused by hyperglycemia. Grape seed proanthocyanidin extract (GSPE) has been reported to possess a broad spectrum of pharmacological and therapeutic properties against oxidative stress. However, its protective effects against diabetic bladder dysfunction have not been clarified. This study focuses on the effects of GSPE on bladder dysfunction in diabetic rats induced by streptozotocin. After 8 weeks of GSPE administration, the bladder function of the diabetic rats was improved significantly, as indicated by both urodynamics analysis and histopathological manifestation. Moreover, the disordered activities of antioxidant enzymes (SOD and GSH-Px) and abnormal oxidative stress levels were partly reversed by treatment with GSPE. Furthermore, the level of apoptosis in the bladder caused by DM was decreased following the administration of GSPE according to the Terminal Deoxynucleotidyl Transferase (TdT)-mediated dUTP Nick-End Labeling (TUNEL) assay. Additionally, GSPE affected the expression of apoptosis-related proteins such as Bax, Bcl-2 and cleaved caspase-3. Furthermore, GSPE showed neuroprotective effects on the bladder of diabetic rats, as shown by the increased expression of nerve growth factor (NGF) and decreased expression of the precursor of nerve growth factor (proNGF). GSPE also activated nuclear erythroid2-related factor2 (Nrf2), which is a key antioxidative transcription factor, with the concomitant elevation of downstream hemeoxygenase-1 (HO-1). These findings suggested that GSPE could ameliorate diabetic bladder dysfunction and decrease the apoptosis of the bladder in diabetic rats, a finding that may be associated with its antioxidant activity and ability to activate the Nrf2 defense pathway.
- Published
- 2015
42. Nampt Expression Decreases Age-Related Senescence in Rat Bone Marrow Mesenchymal Stem Cells by Targeting Sirt1
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Yingai Shi, Yan Li, Yulin Li, Yue Yang, Cao Ma, Xu He, Lin Lin, and Chenchen Pi
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RNA viruses ,0301 basic medicine ,Aging ,Physiology ,medicine.medical_treatment ,Cell ,Nicotinamide phosphoribosyltransferase ,lcsh:Medicine ,Apoptosis ,Pathology and Laboratory Medicine ,Biochemistry ,chemistry.chemical_compound ,Spectrum Analysis Techniques ,Piperidines ,Sirtuin 1 ,Animal Cells ,Medicine and Health Sciences ,Nicotinamide Phosphoribosyltransferase ,lcsh:Science ,Cellular Senescence ,Staining ,Multidisciplinary ,Cell Death ,biology ,Stem Cells ,Age Factors ,Cell Staining ,Stem-cell therapy ,Flow Cytometry ,Cell biology ,Nucleic acids ,medicine.anatomical_structure ,Cell Processes ,Spectrophotometry ,Medical Microbiology ,Viral Pathogens ,Viruses ,Cytophotometry ,Cellular Types ,Pathogens ,Stem cell ,Cell aging ,Research Article ,Senescence ,Down-Regulation ,Bone Marrow Cells ,Research and Analysis Methods ,Microbiology ,03 medical and health sciences ,Retroviruses ,Genetics ,medicine ,Animals ,Microbial Pathogens ,Acrylamides ,Lentivirus ,lcsh:R ,Mesenchymal stem cell ,Organisms ,Biology and Life Sciences ,Mesenchymal Stem Cells ,Cell Biology ,DNA ,Rats ,030104 developmental biology ,chemistry ,Specimen Preparation and Treatment ,biology.protein ,DNA damage ,lcsh:Q ,Physiological Processes ,Organism Development ,Developmental Biology - Abstract
Senescence restricts the development of applications involving mesenchymal stem cells (MSCs) in research fields, such as tissue engineering, and stem cell therapeutic strategies. Understanding the mechanisms underlying natural aging processes may contribute to the development of novel approaches to preventing age-related diseases or slowing individual aging processes. Nampt is a rate-limiting NAD biosynthetic enzyme that plays critical roles in energy metabolism, cell senescence and maintaining life spans. However, it remains unknown whether Nampt influences stem cell senescence. In this study, the function of Nampt was investigated using a rat model of natural aging. Our data show that Nampt expression was significantly lower in MSCs obtained from aged rats than in those obtained from young rats during physiological aging. Reducing the level of Nampt in aged MSCs resulted in lower intracellular concentrations of NAD+ and downregulated Sirt1 expression and activity. After the Nampt inhibitor FK866 was added, young MSCs were induced to become aged cells. The enhanced senescence was correlated with NAD+ depletion and Sirt1 activity attenuation. In addition, Nampt overexpression attenuated cell senescence in aged MSCs. Our findings provide a new explanation for the mechanisms underlying stem cell senescence and a novel target for delaying stem cell senescence and preventing and treating age-related diseases.
- Published
- 2017
43. Lower intensified target LDL-c level of statin therapy results in a higher risk of incident diabetes: a meta-analysis
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Pin Wang, Shaohua Wang, Yue Yang, Rongrong Cai, Wenqing Xia, Yang Yuan, Yi Zhou, Rong Huang, and Haixia Sun
- Subjects
medicine.medical_specialty ,Statin ,medicine.drug_class ,Cardiology ,lcsh:Medicine ,Biochemistry ,chemistry.chemical_compound ,Endocrinology ,Pharmacotherapy ,Risk Factors ,Internal medicine ,Diabetes mellitus ,Diabetes Mellitus ,Medicine and Health Sciences ,Humans ,Medicine ,lcsh:Science ,Diabetic Endocrinology ,Multidisciplinary ,business.industry ,Cholesterol ,lcsh:R ,Biology and Life Sciences ,Cholesterol, LDL ,Odds ratio ,medicine.disease ,Lipids ,Confidence interval ,Type 2 Diabetes ,chemistry ,Cardiovascular Diseases ,Metabolic Disorders ,Meta-analysis ,lipids (amino acids, peptides, and proteins) ,lcsh:Q ,Statin therapy ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,business ,Research Article - Abstract
Background A recent meta-analysis has reported that intensive-dose statin drug increases the risk of incident diabetes. However, doubling of the statin dose generates only a further 6% decrease in low-density lipoprotein cholesterol (LDL-c) on average. This study aimed to determine whether statin therapy with lower intensive-target LDL-c level contributes to higher risk of new-onset diabetes. Methods Medline, Embase, and the Cochrane Central Register of Controlled Trials were searched for randomized controlled endpoint trials of statins conducted from 1966 to 2012. We included trials with more than 1000 participants who were followed up for at least 2 years. The included trials were stratified by the target LDL-c level. I2 statistic was used to measure heterogeneity between trials. We further calculated risk estimates with random-effect meta-analysis. Meta-regression was used to identify the potential risk factors of statin-induced diabetes. Results Fourteen trials with a total of 95 102 non-diabetic participants were included. The risks elevated by 33% [odds ratio (OR) = 1.33; 95% confidence interval (CI) 1.14-1.56; I(2) = 7.7%] and 16% (OR = 1.16; 95% CI 1.06-1.28; I(2)= 0.0%) when the intensified target LDL-c levels were ≤ 1.8 mmol/L and 1.8-2.59 mmol/L, respectively. The risk of incident diabetes did not increase when the target LDL-c level was ≥ 2.59 mmol/L. Apart from age, female, and baseline level of total cholesterol, meta-regression analysis showed that the target and baseline levels of LDL-c and relative LDL-c reduction were predictors of statin-induced diabetes. Conclusion A lower intensified target LDL-c level of statin therapy resulted in a higher risk of incident diabetes.
- Published
- 2014
44. Understanding a substrate's product regioselectivity in a family of enzymes: a case study of acetaminophen binding in cytochrome P450s
- Author
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Sergio E. Wong, Yue Yang, and Felice C. Lightstone
- Subjects
Molecular Conformation ,lcsh:Medicine ,Plasma protein binding ,Molecular Dynamics ,Biochemistry ,Substrate Specificity ,Cytochrome P-450 CYP2A6 ,Protein structure ,Computational Chemistry ,Cytochrome P-450 Enzyme System ,Stereochemistry ,Catalytic Domain ,Macromolecular Structure Analysis ,Cytochrome P-450 CYP3A ,lcsh:Science ,Multidisciplinary ,biology ,Molecular Structure ,Chemistry ,digestive, oral, and skin physiology ,Regioselectivity ,Cytochrome P-450 CYP2E1 ,Stereoisomerism ,Enzymes ,Molecular Docking Simulation ,Molecular Mechanics ,Thermodynamics ,Umbrella sampling ,Research Article ,Computer Modeling ,Protein Binding ,Protein Structure ,Molecular Dynamics Simulation ,Enzyme catalysis ,Molecular recognition ,Cytochrome P-450 CYP1A2 ,Humans ,Biology ,Acetaminophen ,Cytochrome P-450 CYP2C9 ,lcsh:R ,Active site ,Computational Biology ,Protein Structure, Tertiary ,Docking (molecular) ,Enzyme Structure ,Computer Science ,biology.protein ,lcsh:Q - Abstract
Product regioselectivity as influenced by molecular recognition is a key aspect of enzyme catalysis. We applied large-scale two-dimensional (2D) umbrella sampling (USP) simulations to characterize acetaminophen (APAP) binding in the active sites of the family of Cytochrome P450 (CYP) enzymes as a case study to show the different regioselectivity exhibited by a single substrate in comparative enzymes. Our results successfully explain the experimentally observed product regioselectivity for all five human CYPs included in this study, demonstrating that binding events play an important role in determining regioselectivity. In CYP2C9 and CYP3A4, weak interactions in an overall large active site cavity result in a fairly small binding free energy difference between APAP reactive binding states, consistent with experimental results that show little preference for resulting metabolites. In contrast, in CYP1A2 and CYP2E1, APAP is strongly restrained by a compact binding pocket, leading to a preferred binding conformation. The calculated binding equilibrium of APAP within the compact active site of CYP2A6 is able to predict the experimentally documented product ratios and is also applied to explain APAP regioselectivity in CYP1A2 and CYP2C9. APAP regioselectivity seems to be related to the selectivity for one binding conformation over another binding conformation as dictated by the size and shape of the active site. Additionally, unlike docking and molecular dynamics (MD), our free energy calculations successfully reproduced a unique APAP pose in CYP3A4 that had been reported experimentally, suggesting this approach is well suited to find the realistic binding pose and the lowest-energy starting structure for studying the chemical reaction step in the future.
- Published
- 2014
45. Identification of gene expression changes from colitis to CRC in the mouse CAC model
- Author
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Qi Zhao, Guangyu Wang, Xin Li, Yanqiao Zhang, Yuyan Gao, Yan mei Yang, Ming Yang, Hui Liu, and Yue Yang
- Subjects
medicine.medical_treatment ,Azoxymethane ,lcsh:Medicine ,Gene Expression ,Biology ,Research and Analysis Methods ,Carcinomas ,Molecular Genetics ,Mice ,Model Organisms ,Adenocarcinomas ,Gene expression ,Gastrointestinal Tumors ,medicine ,Genetics ,Cancer Genetics ,Medicine and Health Sciences ,Cancer Detection and Diagnosis ,Animals ,KEGG ,lcsh:Science ,Gene ,Tumor microenvironment ,Mice, Inbred BALB C ,Multidisciplinary ,Microarray analysis techniques ,lcsh:R ,Dextran Sulfate ,Wnt signaling pathway ,Colon Adenocarcinoma ,Biology and Life Sciences ,Cancers and Neoplasms ,Animal Models ,Colitis ,Gene Expression Regulation, Neoplastic ,Disease Models, Animal ,Cytokine ,Oncology ,Immunology ,Cancer research ,lcsh:Q ,Female ,Cytokine receptor ,Colorectal Neoplasms ,Cancer Prevention ,Genome-Wide Association Study ,Protein Binding ,Research Article - Abstract
A connection between colorectal carcinogenesis and inflammation is well known, but the underlying molecular mechanisms have not been elucidated. Chemically induced colitis-associated cancer (CAC) is an outstanding mouse model for studying the link between inflammation and cancer. Additionally, the CAC model is used for examining novel diagnostic, prognostic, and predictive markers for use in clinical practice. Here, a CAC model was established in less than 100 days using azoxymethane (AOM) with dextran sulfate sodium salt (DSS) in BALB/c mice. We examined the mRNA expression profiles of three groups: control untreated mice (K), DSS-induced chronic colitis mice (D), and AOM/DSS-induced CAC (AD) mice. We identified 6301 differentially expressed genes (DEGs) among the three groups, including 93 persistently upregulated genes and 139 persistently downregulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses revealed that the most persistent DEGs were significantly enriched in metabolic or inflammatory components in the tumor microenvironment. Furthermore, several associated DEGs were identified as potential DEGs by protein-protein interaction (PPI) network analysis. We selected 14 key genes from the DEGs and potential DEGs for further quantitative real-time PCR (qPCR) verification. Six persistently upregulated, 3 persistently downregulated DEGs, and the other 3 genes showed results consistent with the microarray data. We demonstrated the regulation of 12 key genes specifically involved in Wnt signaling, cytokine and cytokine receptor interactions, homeostasis, and tumor-associated metabolism during colitis-associated CRC. Our results suggest that a close relationship between metabolic and inflammatory mediators of the tumor microenvironment is present in CAC.
- Published
- 2013
46. Decreased Thalamocortical Functional Connectivity after 36 Hours of Total Sleep Deprivation: Evidence from Resting State fMRI
- Author
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Wei Ni, Xiao Jin, Yongcong Shao, Yue Yang, Enmao Ye, Lubin Wang, Bo Wen, Zheng Yang, and Dewen Hu
- Subjects
Adult ,endocrine system ,Time Factors ,Adolescent ,Rest ,Thalamus ,lcsh:Medicine ,Young Adult ,medicine ,Humans ,Prefrontal cortex ,lcsh:Science ,Cerebral Cortex ,Multidisciplinary ,medicine.diagnostic_test ,Resting state fMRI ,business.industry ,lcsh:R ,Parietal lobe ,Magnetic Resonance Imaging ,Sleep deprivation ,medicine.anatomical_structure ,nervous system ,Cerebral cortex ,Sleep Deprivation ,Wakefulness ,lcsh:Q ,medicine.symptom ,Nerve Net ,Functional magnetic resonance imaging ,business ,Neuroscience ,Research Article - Abstract
Objectives: The thalamus and cerebral cortex are connected via topographically organized, reciprocal connections, which hold a key function in segregating internally and externally directed awareness information. Previous task-related studies have revealed altered activities of the thalamus after total sleep deprivation (TSD). However, it is still unclear how TSD impacts on the communication between the thalamus and cerebral cortex. In this study, we examined changes of thalamocortical functional connectivity after 36 hours of total sleep deprivation by using resting state function MRI (fMRI). Materials and Methods: Fourteen healthy volunteers were recruited and performed fMRI scans before and after 36 hours of TSD. Seed-based functional connectivity analysis was employed and differences of thalamocortical functional connectivity were tested between the rested wakefulness (RW) and TSD conditions. Results: We found that the right thalamus showed decreased functional connectivity with the right parahippocampal gyrus, right middle temporal gyrus and right superior frontal gyrus in the resting brain after TSD when compared with that after normal sleep. As to the left thalamus, decreased connectivity was found with the right medial frontal gyrus, bilateral middle temporal gyri and left superior frontal gyrus. Conclusion: These findings suggest disruptive changes of the thalamocortical functional connectivity after TSD, which may lead to the decline of the arousal level and information integration, and subsequently, influence the human cognitive functions.
- Published
- 2013
47. The Efficacy of a Nurse-Led Disease Management Program in Improving the Quality of Life for Patients with Chronic Kidney Disease: A Meta-Analysis
- Author
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Chen, Chong-Cheng, primary, Chen, Yi, additional, Liu, Xia, additional, Wen, Yue, additional, Ma, Deng-Yan, additional, Huang, Yue-Yang, additional, Pu, Li, additional, Diao, Yong-Shu, additional, and Yang, Kun, additional
- Published
- 2016
- Full Text
- View/download PDF
48. Exploring the Antibacterial and Antifungal Potential of Jellyfish-Associated Marine Fungi by Cultivation-Dependent Approaches
- Author
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Yue, Yang, primary, Yu, Huahua, additional, Li, Rongfeng, additional, Xing, Ronge, additional, Liu, Song, additional, and Li, Pengcheng, additional
- Published
- 2015
- Full Text
- View/download PDF
49. Regional Brain Structural Abnormality in Meal-Related Functional Dyspepsia Patients: A Voxel-Based Morphometry Study
- Author
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Fanrong Liang, Danhua Zhang, Sheng-Feng Lu, Yuan Chen, Fang Zeng, Ying Li, Guangyu Zhou, Jixin Liu, Yong Tang, Jie Tian, Lei Lan, Qiyong Gong, Jinbo Sun, Yue Yang, Shu-Guang Yu, Wei Qin, and Xin Gao
- Subjects
Male ,medicine.medical_specialty ,Pathology ,Anatomy and Physiology ,Population ,lcsh:Medicine ,Prefrontal Cortex ,Neuroimaging ,Gastroenterology and Hepatology ,Anxiety ,Gastroenterology ,Brain mapping ,Gyrus Cinguli ,Neurological System ,Young Adult ,Internal medicine ,medicine ,Humans ,Dyspepsia ,Prefrontal cortex ,education ,lcsh:Science ,Biology ,education.field_of_study ,Brain Mapping ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,Depression ,lcsh:R ,Brain ,Magnetic resonance imaging ,Voxel-based morphometry ,Magnetic Resonance Imaging ,Frontal Lobe ,Neuroanatomy ,Frontal lobe ,Neurology ,Medicine ,lcsh:Q ,Female ,Abnormality ,Molecular Neuroscience ,Nerve Net ,business ,Research Article ,Neuroscience - Abstract
BACKGROUND AND AIMS: Brain dysfunction in functional dyspepsia (FD) has been identified by multiple neuroimaging studies. This study aims to investigate the regional gray matter density (GMD) changes in meal-related FD patients and their correlations with clinical variables, and to explore the possible influence of the emotional state on FD patients's brain structures. METHODS: Fifty meal-related FD patients and forty healthy subjects (HS) were included and underwent a structural magnetic resonance imaging scan. Voxel-based morphometry analysis was employed to identify the cerebral structure alterations in meal-related FD patients. Regional GMD changes' correlations with the symptoms and their durations, respectively, have been analyzed. RESULTS: Compared to the HS, the meal-related FD patients showed a decreased GMD in the bilateral precentral gyrus, medial prefrontal cortex (MPFC), anterior cingulate cortex (ACC) and midcingulate cortex (MCC), left orbitofrontal cortex (OFC) and right insula (p50). After controlling for anxiety and depression, the meal-related FD patients showed a decreased GMD in the bilateral middle frontal gyrus, left MCC, right precentral gyrus and insula (p50). Before controlling psychological factors, the GMD decreases in the ACC were negatively associated with the symptom scores of the Nepean Dyspepsia Index (NDI) (r = -0.354, p = 0.048, Bonferroni correction) and the duration of FD (r = -0.398, p = 0.02, Bonferroni correction) respectively. CONCLUSIONS: The regional GMD of meal-related FD patients, especially in the regions of the homeostatic afferent processing network significantly differed from that of the HS, and the psychological factors might be one of the essential factors significantly affecting the regional brain structure of meal-related FD patients.
- Published
- 2013
50. Hypoxia-inducible factor-1α and interleukin 33 form a regulatory circuit to perpetuate the inflammation in rheumatoid arthritis
- Author
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Zhanguo Li, Rong Mu, Rulin Jia, Dong-yue Yang, Xiaoxu Ma, Fanlei Hu, Yingni Li, Yun Li, Lianjie Shi, Chun Li, and Jiaxin Zhu
- Subjects
medicine.medical_treatment ,Arthritis ,lcsh:Medicine ,Rheumatoid Arthritis ,Inflammation ,Immunological Signaling ,Models, Biological ,Autoimmune Diseases ,Proinflammatory cytokine ,Arthritis, Rheumatoid ,Rheumatology ,Synovial Fluid ,Molecular Cell Biology ,medicine ,Humans ,Synovial fluid ,lcsh:Science ,Biology ,Cellular Stress Responses ,Multidisciplinary ,business.industry ,Interleukins ,lcsh:R ,Immunity ,Interleukin ,Fibroblasts ,Signaling in Selected Disciplines ,Hypoxia-Inducible Factor 1, alpha Subunit ,Interleukin-33 ,medicine.disease ,Interleukin 33 ,Cytokine ,Gene Expression Regulation ,Gene Knockdown Techniques ,Immune System ,Rheumatoid arthritis ,Immunology ,Medicine ,Cytokines ,Clinical Immunology ,lcsh:Q ,medicine.symptom ,business ,Signal Transduction ,Research Article - Abstract
Hyperplasia of synovial fibroblasts, infiltration with inflammatory cytokines, and tissue hypoxia are the major characteristics of rheumatoid arthritis (RA). Interleukin 33 (IL-33) is a newly identified inflammatory cytokine exacerbating the disease severity of RA. Hypoxia-inducible factor-1α (HIF-1α) showed increased expression in RA synovium and could regulate a number of inflammatory cytokine productions. Nevertheless, its correlation with IL-33 remains largely unknown. Here, we showed that elevated levels of IL-33 were demonstrated in RA patient synovial fluids, with upregulated expression of HIF-1α and IL-33 in the synovial fibroblasts. Knocking down HIF-1α compromised IL-33 expression in rheumatoid arthritis synovial fibroblasts (RASF), while enforcing HIF-1α expression in RASF substantially upregulated IL-33 levels. HIF-1α promoted the activation of the signalling pathways controlling IL-33 production, particularly the p38 and ERK pathways. Moreover, we showed for the first time that IL-33 in turn could induce more HIF-1α expression in RASF, thus forming a HIF-1α/IL-33 regulatory circuit that would perpetuate the inflammatory process in RA. Targeting this pathological pathway and HIF-1α may provide new therapeutic strategies for overcoming the persistent and chronic inflammatory disease.
- Published
- 2013
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