33 results on '"Yanli Wang"'
Search Results
2. Changes in diet, exercise and psychology of the quarantined population during the COVID-19 outbreak in Shanghai.
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Li Qiu, Chenchen Li, Wen He, Xuelian Yin, Lin Zhan, Junfeng Zhang, and Yanli Wang
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Medicine ,Science - Abstract
BackgroundIn March 2022, a severe outbreak of COVID-19 broke out in Shanghai, with the virus spreading rapidly. In the most severe two months, more than 50,000 people were diagnosed with COVID-19. For this reason, Shanghai adopted three-district hierarchical management, requiring corresponding people to stay at home to contain the spread of the virus. Due to the requirements of prevention and control management, the diet, exercise and mental health of the corresponding population are affected to a certain extent.ObjectivesThis article aimed to understand the population in the diet, exercise and psychological changes.MethodsThis study carried out the research by distributing the electronic questionnaire and carried out the statistical analysis.ResultsPeople reduced the intake of vegetables and fruits (P = 0.000ConclusionIn terms of psychological state, people have some depression, anxiety and easy to feel tired after lockdown. This study can also provide reference for policy adjustment and formulation of normalized epidemic management in the future.
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- 2023
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3. Impact of social class on health: The mediating role of health self-management.
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Xiaoyong Hu, Tiantian Wang, Duan Huang, Yanli Wang, and Qiong Li
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Medicine ,Science - Abstract
BackgroundStudies have explored the relationship between social class and health for decades. However, the underlying mechanism between the two remains not fully understood. This study aimed to explore whether health self-management had a mediating role between social class and health under the framework of Socio-cultural Self Model.Methods663 adults, randomly sampled from six communities in Southwest China, completed the survey for this study. Social class was assessed using individuals' income, education, occupation. Health self-management was assessed through evaluation of the health self-management behavior, health self-management cognition, health self-management environment. Physical health and mental health were measured by the Chinese version of Short-Form (36-item) Health Survey, which contains Physical Functioning, Role-Physical, Role-Emotional, Vitality, Mental Health, Social Function, Bodily Pain and General Health. Pearson's correlation was used to examine the associations between major variables. Mediation analyses were performed to explore the mediating role of health self-management.ResultsSocial class positively predicted self-rated health. The lower the social class, the lower the self-reported physical and mental health. Health self-management partially mediated the relationship between social class and self-rated health. That is, the health self-management ability of the lower class, such as access to healthy and nutritious food and evaluate their own health status, is worse than that of the higher class, which leads to physical and mental health inequality between the high and the low classes.ConclusionHealth self-management mediated the relationship between social class and health. Promoting health self-management abilities are conducive to improving both physical and mental health.
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- 2021
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4. De novo transcriptome analysis of Viola ×wittrockiana exposed to high temperature stress.
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Xiaohua Du, Xiaopei Zhu, Yaping Yang, Yanli Wang, Paul Arens, and Huichao Liu
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Medicine ,Science - Abstract
Around the world, pansies are one of the most popular garden flowers, but they are generally sensitive to high temperatures, and this limits the practicality of planting them during the warmest days of the year. However, a few pansy germplasms with improved heat tolerance have been discovered or bred, but the mechanisms of their heat resistance are not understood. In this study, we investigated the transcript profiles of a heat-tolerant pansy inbred line, DFM16, in response to high temperatures using RNAseq. Approximately 55.48 Gb of nucleotide data were obtained and assembled into 167,576 unigenes with an average length of 959 bp, of which, 5,708 genes were found to be differentially expressed after heat treatments. Real-time qPCR was performed to validate the expression profiles of the selected genes. Nine metabolic pathways were found to be significantly enriched, in the analysis of the differentially expressed genes. Several potentially interesting genes that encoded putative transcription regulators or key components involving heat shock protein (HSP), heat shock transcription factors (HSF), and antioxidants biosynthesis, were identified. These genes were highlighted to indicate their significance in response to heat stress and will be used as candidate genes to improve pansy heat-tolerance in the future.
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- 2019
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5. Determinants of Divergent Adaptive Immune Responses after Airway Sensitization with Ligands of Toll-Like Receptor 5 or Toll-Like Receptor 9.
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Linda M Lee, Ming Ji, Meenal Sinha, Matthew B Dong, Xin Ren, Yanli Wang, Clifford A Lowell, Sankar Ghosh, Richard M Locksley, and Anthony L DeFranco
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Medicine ,Science - Abstract
Excessive type 2 helper T cell responses to environmental antigens can cause immunopathology such as asthma and allergy, but how such immune responses are induced remains unclear. We studied this process in the airways by immunizing mice intranasally with the antigen ovalbumin together with either of two Toll-like receptor (TLR) ligands. We found the TLR5 ligand flagellin promoted a type 2 helper T cell response, whereas, a TLR9 ligand CpG oligodeoxyribonucleotide (ODN) promoted a type 1 helper T cell response. CpG ODN induced mRNA encoding interleukin (IL)-12 p40, whereas, flagellin caused IL-33 secretion and induced mRNAs encoding IL-1 and thymic stromal lymphopoietin (TSLP). By using mice deficient in the TLR and IL-1R signaling molecule, myeloid differentiation primary response 88 (MyD88), in conventional dendritic cells (cDCs) and alveolar macrophages (AMs), and by cell sorting different lung populations after 2 hours of in vivo stimulation, we characterized the cell types that rapidly produced inflammatory cytokines in response to TLR stimulation. CpG ODN was likely recognized by TLR9 on cDCs and AMs, which made mRNA encoding IL-12. IL-12 was necessary for the subsequent innate and adaptive interferon-γ production. In contrast, flagellin stimulated multiple cells of hematopoietic and non-hematopoietic origin, including AMs, DCs, monocytes, and lung epithelial cells. AMs were largely responsible for IL-1α, whereas lung epithelial cells made TSLP. Multiple hematopoietic cells, including AMs, DCs, and monocytes contributed to other cytokines, including IL-1β and TNFα. MyD88-dependent signals, likely through IL-1R and IL-33R, and MyD88-independent signals, likely from TSLP, were necessary in cDCs for promotion of the early IL-4 response by CD4 T cells in the draining lymph node. Thus, the cell types that responded to TLR ligands were a critical determinant of the innate cytokines produced and the character of the resulting adaptive immune response in the airways.
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- 2016
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6. An S-(hydroxymethyl)glutathione dehydrogenase is involved in conidiation and full virulence in the rice blast fungus Magnaporthe oryzae.
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Zhen Zhang, Jiaoyu Wang, Rongyao Chai, Haiping Qiu, Hua Jiang, Xueqin Mao, Yanli Wang, Fengquan Liu, and Guochang Sun
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Medicine ,Science - Abstract
Magnaporthe oryzae is a hemibiotrophic fungal pathogen that causes rice blast disease. A compatible interaction requires overcoming plant defense responses to initiate colonization during the early infection process. Nitric oxide (NO) plays important roles in defense responses during host-pathogen interactions. Microbes generally protect themselves against NO-induced damage by using enzymes. Here, we characterized an S-(hydroxymethyl)-glutathione dehydrogenase gene in M. oryzae, MoSFA1, the homologs of which are involved in NO metabolism by specifically catalyzing the reduction of S-nitrosoglutathione (GSNO) in yeasts and plants. As expected from the activities of S-(hydroxymethyl)glutathione dehydrogenase in formaldehyde detoxification and GSNO reduction, MoSFA1 deletion mutants were lethal in formaldehyde containing medium, sensitive to exogenous NO and exhibited a higher level of S-nitrosothiols (SNOs) than that of the wild type. Notably, the mutants showed severe reduction of conidiation and appressoria turgor pressure, as well as significantly attenuated the virulence on rice cultivar CO-39. However, the virulence of MoSFA1 deletion mutants on wounded rice leaf was not affected. An infection assay on barley leaf further revealed that MoSFA1 deletion mutants exhibited a lower infection rate, and growth of infectious hyphae of the mutants was retarded not only in primary infected cells but also in expansion from cell to cell. Furthermore, barley leaf cell infected by MoSFA1 deletion mutants exhibited a stronger accumulation of H2O2 at 24 and 36 hpi. MoSFA1 deletion mutants displayed hypersensitivity to different oxidants, reduced activities of superoxide dismutases and peroxidases, and lower glutathione content in cells, compared with the wild type. These results imply that MoSFA1-mediated NO metabolism is important in redox homeostasis in response to development and host infection of M. oryzae. Taken together, this work identifies that MoSFA1 is required for conidiation and contributes to virulence in the penetration and biotrophic phases in M. oryzae.
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- 2015
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7. One of Three Pex11 Family Members Is Required for Peroxisomal Proliferation and Full Virulence of the Rice Blast Fungus Magnaporthe oryzae.
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Jiaoyu Wang, Ling Li, Zhen Zhang, Haiping Qiu, Dongmei Li, Yuan Fang, Hua Jiang, Rong Yao Chai, Xueqin Mao, Yanli Wang, and Guochang Sun
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Medicine ,Science - Abstract
Peroxisomes play important roles in metabolisms of eukaryotes and infection of plant fungal pathogens. These organelles proliferate by de novo formation or division in response to environmental stimulation. Although the assembly of peroxisomes was documented in fungal pathogens, their division and its relationship to pathogenicity remain obscure. In present work, we analyzed the roles of three Pex11 family members in peroxisomal division and pathogenicity of the rice blast fungus Magnaporthe oryzae. Deletion of MoPEX11A led to fewer but enlarged peroxisomes, and impaired the separation of Woronin bodies from peroxisomes, while deletion of MoPEX11B or MoPEX11C put no evident impacts to peroxisomal profiles. MoPEX11A mutant exhibited typical peroxisome related defects, delayed conidial germination and appressoria formation, and decreased appressorial turgor and host penetration. As a result, the virulence of MoPEX11A mutant was greatly reduced. Deletion of MoPEX11B and MoPEX11C did not alter the virulence of the fungus. Further, double or triple deletions of the three genes were unable to enhance the virulence decrease in MoPEX11A mutant. Our data indicated that MoPEX11A is the main factor modulating peroxisomal division and is required for full virulence of the fungus.
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- 2015
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8. Inward Rectifier K+ Currents Are Regulated by CaMKII in Endothelial Cells of Primarily Cultured Bovine Pulmonary Arteries.
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Lihui Qu, Lei Yu, Yanli Wang, Xin Jin, Qianlong Zhang, Ping Lu, Xiufeng Yu, Weiwei Zhong, Xiaodong Zheng, Ningren Cui, Chun Jiang, and Daling Zhu
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Medicine ,Science - Abstract
Endothelium lines the interior surface of vascular walls and regulates vascular tones. The endothelial cells sense and respond to chemical and mechanical stimuli in the circulation, and couple the stimulus signals to vascular smooth muscles, in which inward rectifier K+ currents (Kir) play an important role. Here we applied several complementary strategies to determine the Kir subunit in primarily cultured pulmonary arterial endothelial cells (PAECs) that was regulated by the Ca2+/calmodulin (CaM)-dependent protein kinase II (CaMKII). In whole-cell voltage clamp, the Kir currents were sensitive to micromolar concentrations of extracellular Ba2+. In excised inside-out patches, an inward rectifier K+ current was observed with single-channel conductance 32.43 ± 0.45 pS and Popen 0.27 ± 0.04, which were consistent with known unitary conductance of Kir 2.1. RT-PCR and western blot results showed that expression of Kir 2.1 was significantly stronger than that of other subtypes in PAECs. Pharmacological analysis of the Kir currents demonstrated that insensitivity to intracellular ATP, pinacidil, glibenclamide, pH, GDP-β-S and choleratoxin suggested that currents weren't determined by KATP, Kir2.3, Kir2.4 and Kir3.x. The currents were strongly suppressed by exposure to CaMKII inhibitor W-7 and KN-62. The expression of Kir2.1 was inhibited by knocking down CaMKII. Consistently, vasodilation was suppressed by Ba2+, W-7 and KN-62 in isolated and perfused pulmonary arterial rings. These results suggest that the PAECs express an inward rectifier K+ current that is carried dominantly by Kir2.1, and this K+ channel appears to be targeted by CaMKII-dependent intracellular signaling systems.
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- 2015
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9. MoPex19, which is essential for maintenance of peroxisomal structure and woronin bodies, is required for metabolism and development in the rice blast fungus.
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Ling Li, Jiaoyu Wang, Zhen Zhang, Yanli Wang, Maoxin Liu, Hua Jiang, Rongyao Chai, Xueqin Mao, Haiping Qiu, Fengquan Liu, and Guochang Sun
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Medicine ,Science - Abstract
Peroxisomes are present ubiquitously and make important contributions to cellular metabolism in eukaryotes. They play crucial roles in pathogenicity of plant fungal pathogens. The peroxisomal matrix proteins and peroxisomal membrane proteins (PMPs) are synthesized in the cytosol and imported post-translationally. Although the peroxisomal import machineries are generally conserved, some species-specific features were found in different types of organisms. In phytopathogenic fungi, the pathways of the matrix proteins have been elucidated, while the import machinery of PMPs remains obscure. Here, we report that MoPEX19, an ortholog of ScPEX19, was required for PMPs import and peroxisomal maintenance, and played crucial roles in metabolism and pathogenicity of the rice blast fungus Magnaporthe oryzae. MoPEX19 was expressed in a low level and Mopex19p was distributed in the cytoplasm and newly formed peroxisomes. MoPEX19 deletion led to mislocalization of peroxisomal membrane proteins (PMPs), as well peroxisomal matrix proteins. Peroxisomal structures were totally absent in Δmopex19 mutants and woronin bodies also vanished. Δmopex19 exhibited metabolic deficiency typical in peroxisomal disorders and also abnormality in glyoxylate cycle which was undetected in the known mopex mutants. The Δmopex19 mutants performed multiple disorders in fungal development and pathogenicity-related morphogenesis, and lost completely the pathogenicity on its hosts. These data demonstrate that MoPEX19 plays crucial roles in maintenance of peroxisomal and peroxisome-derived structures and makes more contributions to fungal development and pathogenicity than the known MoPEX genes in the rice blast fungus.
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- 2014
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10. Synergistic association between two alcohol metabolism relevant genes and coronary artery disease among Chinese hypertensive patients.
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Yuefei Wang, Fengxia Du, Hongye Zhao, Xiaohong Yu, Jun Liu, Yu Xiao, Changzhu Lu, Xue Li, Yanli Wang, Bin Wang, and Wenquan Niu
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Medicine ,Science - Abstract
Coronary artery disease (CAD) is a multifactorial and polygenic disease. The aim of this study was to examine the association between six polymorphisms of four alcohol metabolism relevant genes (ADH1B, ADH1C, ALDH1b1, ALDH2) and the risk of CAD in Han Chinese.This was a hospital-based case-control study involving 1365 hypertensive patients. All study subjects were angiographically confirmed. Genotypes were determined with ligase detection reaction method. There was no observable deviation from the Hardy-Weinberg equilibrium for six examined polymorphisms in controls. The genotype and allele distributions of ALDH1b1 rs2073478 and ALDH2 rs671 polymorphisms differed significantly between the two groups (P≤0.005), even after the Bonferroni correction. The most common allele combination was A-C-C-G-C-G (alleles in order of rs1229984, rs1693482, rs2228093, rs2073478, rs886205, rs671) and its frequency was slightly higher in controls than in CAD patients (P = 0.067). After assigning the most common allele combination as a reference, allele combination A-C-C-T-C-A, which simultaneously possessed the risk alleles of rs2073478 and rs671 polymorphisms, was associated with a 1.80-fold greater risk of CAD. Further, a two-locus model including rs2073478 and rs671 that had a maximal testing accuracy of 0.598 and a cross-validation consistency of 10 (P = 0.008) was deemed as the overall best MDR model, which was further validated by classical Logistic regression model.Our findings provide clear evidence for both individual and interactive associations of ALDH1b1 and ALDH2 genes with the development of CAD in Han Chinese.
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- 2014
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11. Urotensin II promotes atherosclerosis in cholesterol-fed rabbits.
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Yafeng Li, Sihai Zhao, Yanli Wang, Yulong Chen, Yan Lin, Ninghong Zhu, Huadong Zheng, Min Wu, Daxing Cheng, Yandong Li, Liang Bai, Jianglin Fan, and Enqi Liu
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Medicine ,Science - Abstract
Urotensin II (UII) is a vasoactive peptide composed of 11 amino acids that has been implicated to contribute to the development of cardiovascular disease. The purpose of this study was to investigate whether UII affects the development of atherosclerosis in cholesterol-fed rabbits. UII was infused for 16 weeks through an osmotic mini-pump into male Japanese White rabbits fed on a high-cholesterol diet. Plasma lipids and body weight were measured every 4 weeks. Aortic atherosclerotic lesions along with cellular components, collagen fibers, matrix metalloproteinase-1 and -9 were examined. Moreover, vulnerability index of atherosclerotic plaques was evaluated. UII infusion significantly increased atherosclerotic lesions within the entire aorta by 21% over the control (P = 0.013). Atherosclerotic lesions were increased by 24% in the aortic arch (P = 0.005), 11% in the thoracic aorta (P = 0.054) and 18% in the abdominal aorta (P = 0.035). These increases occurred without changes in plasma levels of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides or body weight. Immunohistochemical staining revealed that macrophages and matrix metalloproteinase-9 were significantly enhanced by 2.2-fold and 1.6-fold in UII group. In vitro studies demonstrated that UII up-regulated the expression of vascular cell adhesion protein-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells, which was inhibited by the UII receptor antagonist urantide. In conclusion, our results showed that UII promotes the development of atherosclerotic lesions and destabilizes atherosclerotic plaques in cholesterol-fed rabbits.
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- 2014
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12. The contributory role of angiotensin receptor-like 1 gene multiple polymorphisms in hypertension among northeastern Han Chinese.
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Ruoshan Liu, Hongye Zhao, Yuefei Wang, Yanli Wang, Changzhu Lu, Yu Xiao, Nan Jia, Bin Wang, and Wenquan Niu
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Medicine ,Science - Abstract
BACKGROUND AND OBJECTIVE: Via direct sequencing, we have recently identified six common polymorphisms in angiotensin receptor-like 1 (AGTRL1) gene, and found only two polymorphisms were significantly associated with hypertension in a family-based analysis on 1,015 southern Han Chinese. Extending our previous work and considering the ubiquity of epistasis in determining disease susceptibility, we, in this study, sought to explore the potential interaction of AGTRL1 gene six polymorphisms with hypertension in a large northeastern Han Chinese population. METHODS AND RESULTS: This was a case-control study involving 1,009 sporadic hypertensive patients and 756 normotensive controls. Data were analyzed by Haplo.Stats and multifactor dimensionality reduction (MDR) softwares. There were no deviations from Hardy-Weinberg equilibrium for all polymorphisms. The genotypes and alleles of rs7119675 and rs11544374 differed significantly between the two groups (P
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- 2014
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13. Clinical features and factors associated with severity and fatality among patients with severe fever with thrombocytopenia syndrome Bunyavirus infection in Northeast China.
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Baocheng Deng, Bo Zhou, Shujun Zhang, Ying Zhu, Leqiang Han, Yingzhi Geng, Zhenan Jin, Hongbo Liu, Donglei Wang, Yitong Zhao, Ying Wen, Wei Cui, Ying Zhou, Qiuhong Gu, Cuiming Sun, Xu Lu, Wen Wang, Yu Wang, Chengbo Li, Yanli Wang, Wenqing Yao, and Pei Liu
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Medicine ,Science - Abstract
BACKGROUND: In 2009, severe fever with thrombocytopenia syndrome virus (SFTSV) was identified as a novel member of the genus phlebovirus in the Bunyaviridae family in China. The detailed clinical features of cases with SFTSV infection have not been well described, and the risk factors for severity among patients and fatality among severe patients remain to be determined. METHODOLOGY/PRINCIPAL FINDINGS: Clinical and laboratory features of 115 hospitalized patients with SFTSV infection during the period from June 2010 to December 2011 in Northeast China were retrospectively reviewed. We assessed the risk factors associated with severity in confirmed cases and fatality in severe cases by multivariate analysis. One hundred and three (89.6%) of 115 patients presented with multiple organ dysfunction, and 22 (19.1%) of 115 proceeded to the stage of life threatening multiple organ failure. Of the 115 patients, 14 fatalities (12.2%) were reported. Multivariate analysis demonstrated that the independent predictors of risk for severity were: albumin ≤ 30 g/l (OR, 8.09; 95% CI, 2.58-25.32), APTT ≥ 66 seconds (OR, 14.28; 95% CI, 3.28-62.24), sodium ≤ 130 mmol/l (OR, 5.44; 95% CI, 1.38-21.40), and presence of neurological manifestations (OR, 7.70; 95% CI, 1.91-31.12). Among patients with severe disease, presence of acute lung injury/acute respiratory distress syndrome (HR, 4.59; 95% CI, 1.48-14.19) and disseminated intravascular coagulation (HR, 4.24; 95% CI, 1.38-13.03) were independently associated with fatality. CONCLUSIONS/SIGNIFICANCE: SFTSV infection may present with more severe symptoms and laboratory abnormalities than hitherto reported. Due to infection with a novel bunyavirus, the patients may sufferer multiple organ dysfunction and die of multiple organ failure. In the clinical assessment of any case of SFTS, independent factors relating to prognosis need to be taken into account by clinicians.
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- 2013
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14. PTS1 peroxisomal import pathway plays shared and distinct roles to PTS2 pathway in development and pathogenicity of Magnaporthe oryzae.
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Jiaoyu Wang, Zhen Zhang, Yanli Wang, Ling Li, Rongyao Chai, Xueqin Mao, Hua Jiang, Haiping Qiu, Xinfa Du, Fucheng Lin, and Guochang Sun
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Medicine ,Science - Abstract
Peroxisomes participate in various important metabolisms and are required in pathogenicity of fungal plant pathogens. Peroxisomal matrix proteins are imported from cytoplasm into peroxisomes through peroxisomal targeting signal 1 (PTS1) or peroxisomal targeting signal 2 (PTS2) import pathway. PEX5 and PEX7 genes participate in the two pathways respectively. The involvement of PEX7 mediated PTS2 import pathway in fungal pathogenicity has been documented, while that of PTS1 remains unclear. Through null mutant analysis of MoPEX5, the PEX5 homolog in Magnaporthe oryzae, we report the crucial roles of PTS1 pathway in the development and host infection in the rice blast fungus, and compared with those of PTS2. We found that MoPEX5 disruption specifically blocked the PTS1 pathway. Δmopex5 was unable to use lipids as sole carbon source and lost pathogenicity completely. Similar as Δmopex7, Δmopex5 exhibited significant reduction in lipid utilization and mobilization, appressorial turgor genesis and H(2)O(2) resistance. Additionally, Δmopex5 presented some distinct defects which were undetected in Δmopex7 in vegetative growth, conidial morphogenesis, appressorial morphogenesis and melanization. The results indicated that the PTS1 peroxisomal import pathway, in addition to PTS2, is required for fungal development and pathogenicity of the rice blast fungus, and also, as a main peroxisomal import pathway, played a more predominant role than PTS2.
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- 2013
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15. Correction: Differential Expression of and Protein Profile of Outer Membrane of Subsp.
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Muhammad Ibrahim, Yu Shi, Hui Qiu, Bin Li, Amara Jabeen, Liping Li, He Liu, Michael Kube, Guanlin Xie, Yanli Wang, Carlos Blondel, Carlos A. Santiviago, Inés Contreras, and Guochang Sun
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Medicine ,Science - Published
- 2013
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16. MiR-146a regulates SOD2 expression in H2O2 stimulated PC12 cells.
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Guohua Ji, Ke Lv, Hailong Chen, Tingmei Wang, Yanli Wang, Dingsheng Zhao, Lina Qu, and Yinghui Li
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Medicine ,Science - Abstract
SOD2 (superoxide dismutase 2) is one of the endogenous antioxidant enzymes that protect against reactive oxygen species. While explorations of SOD2 expression regulation are mainly focused on transcriptional and post-translational activation, there are few reports about the post-transcriptional regulation of SOD2. MicroRNAs (miRNAs) are 21nt-25nt (nucleotide) small noncoding RNAs that have emerged as indispensable regulators of gene expression. Here we show that miR-146a, a widely expressed miRNA, is up-regulated by H2O2-induced stress. By sequence analysis we found a binding site for miR-146a in the sod2 mRNA 3'UTR, and a luciferase reporter assay confirmed that miR-146a can interact with this sod2 regulatory region. Our results further show that miR-146a could down-regulate the SOD2 protein expression, and antisense-miR-146a could reverse the decrease of both the SOD2 level and cell viability in H2O2 treated PC12 cells. In conclusion, here we have identified a novel function of miR-146a in the post-transcriptional regulation of SOD2 expression.
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- 2013
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17. Differential expression of in vivo and in vitro protein profile of outer membrane of Acidovorax avenae subsp. avenae.
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Muhammad Ibrahim, Yu Shi, Hui Qiu, Bin Li, Amara Jabeen, Liping Li, He Liu, Michael Kube, Guanlin Xie, Yanli Wang, Carlos Blondel, Carlos A Santiviago, Ines Contreras, and Guochang Sun
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Medicine ,Science - Abstract
Outer membrane (OM) proteins play a significant role in bacterial pathogenesis. In this work, we examined and compared the expression of the OM proteins of the rice pathogen Acidovorax avenae subsp. avenae strain RS-1, a Gram-negative bacterium, both in an in vitro culture medium and in vivo rice plants. Global proteomic profiling of A. avenae subsp. avenae strain RS-1 comparing in vivo and in vitro conditions revealed the differential expression of proteins affecting the survival and pathogenicity of the rice pathogen in host plants. The shotgun proteomics analysis of OM proteins resulted in the identification of 97 proteins in vitro and 62 proteins in vivo by mass spectrometry. Among these OM proteins, there is a high number of porins, TonB-dependent receptors, lipoproteins of the NodT family, ABC transporters, flagellins, and proteins of unknown function expressed under both conditions. However, the major proteins such as phospholipase and OmpA domain containing proteins were expressed in vitro, while the proteins such as the surface anchored protein F, ATP-dependent Clp protease, OmpA and MotB domain containing proteins were expressed in vivo. This may indicate that these in vivo OM proteins have roles in the pathogenicity of A. avenae subsp. avenae strain RS-1. In addition, the LC-MS/MS identification of OmpA and MotB validated the in silico prediction of the existance of Type VI secretion system core components. To the best of our knowledge, this is the first study to reveal the in vitro and in vivo protein profiles, in combination with LC-MS/MS mass spectra, in silico OM proteome and in silico genome wide analysis, of pathogenicity or plant host required proteins of a plant pathogenic bacterium.
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- 2012
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18. Cytokine and chemokine levels in patients with severe fever with thrombocytopenia syndrome virus.
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Baocheng Deng, Shujun Zhang, Yingzhi Geng, Yuzhong Zhang, Yuncheng Wang, Wenqing Yao, Ying Wen, Wei Cui, Ying Zhou, Qiuhong Gu, Wen Wang, Yu Wang, Zhen Shao, Yanli Wang, Chengbo Li, Donglei Wang, Yitong Zhao, and Pei Liu
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Medicine ,Science - Abstract
BACKGROUND: Severe fever with thrombocytopenia syndrome virus (SFTSV), which can cause hemorrhagic fever-like illness, is a newly discovered bunyavirus in China. The pathogenesis of SFTSV infection is poorly understood. However, it has been suggested that immune mechanisms, including cytokines and chemokines, play an important role in disease pathogenesis. In the present study, we investigated host cytokine and chemokine profiles in serum samples of patients with SFTSV infection from Northeast China and explored a possible correlation between cytokine levels and disease severity. METHODS AND PRINCIPAL FINDINGS: Acute phase serum samples from 40 patients, diagnosed with SFTSV infection were included. Patients were divided into two groups--severe or non-severe--based on disease severity. Levels of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, interleukin-6, interferon (IFN)-γ, IFN- γ-induced protein (IP)-10 and RANTES were measured in the serum samples with commercial ELISAs. Statistical analysis showed that increases in TNF-α, IP-10 and IFN-γ were associated with disease severity. CONCLUSIONS: We suggest that a cytokine-mediated inflammatory response, characterized by cytokine and chemokine production imbalance, might be in part responsible for the disease progression of patients with SFTSV infection.
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- 2012
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19. Correction: A handy method to remove bacterial contamination from fungal cultures
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Guochang Sun, Yanli Wang, Zhen Zhang, Jiaoyu Wang, Xiao-Xiao Shi, and Haiping Qiu
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Multidisciplinary ,business.industry ,Science ,Medicine ,Contamination ,business ,Microbiology - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0224635.].
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- 2020
20. Impact of social class on health: The mediating role of health self-management
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Duan Huang, Yanli Wang, Tiantian Wang, Xiaoyong Hu, and Qiong Li
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Male ,Gerontology ,Economics ,Health Status ,Social Sciences ,Global Health ,Vitality ,Health informatics ,0302 clinical medicine ,Sociology ,Medicine and Health Sciences ,Public and Occupational Health ,030212 general & internal medicine ,media_common ,Schools ,Multidisciplinary ,05 social sciences ,Cognition ,Middle Aged ,Socioeconomic Aspects of Health ,Professions ,Mental Health ,Working class ,Medicine ,Educational Status ,Female ,Economic Development ,Behavioral and Social Aspects of Health ,Psychology ,Research Article ,Adult ,Mediation (statistics) ,Adolescent ,Science ,media_common.quotation_subject ,Social class ,050105 experimental psychology ,Education ,Young Adult ,03 medical and health sciences ,Development Economics ,Mental Health and Psychiatry ,Humans ,Social Stratification ,0501 psychology and cognitive sciences ,Aged ,business.industry ,Self-Management ,Health Status Disparities ,Health Surveys ,Social stratification ,Mental health ,Health Care ,Social Class ,People and Places ,Population Groupings ,Self Report ,business - Abstract
Background Studies have explored the relationship between social class and health for decades. However, the underlying mechanism between the two remains not fully understood. This study aimed to explore whether health self-management had a mediating role between social class and health under the framework of Socio-cultural Self Model. Methods 663 adults, randomly sampled from six communities in Southwest China, completed the survey for this study. Social class was assessed using individuals’ income, education, occupation. Health self-management was assessed through evaluation of the health self-management behavior, health self-management cognition, health self-management environment. Physical health and mental health were measured by the Chinese version of Short-Form (36-item) Health Survey, which contains Physical Functioning, Role-Physical, Role-Emotional, Vitality, Mental Health, Social Function, Bodily Pain and General Health. Pearson’s correlation was used to examine the associations between major variables. Mediation analyses were performed to explore the mediating role of health self-management. Results Social class positively predicted self-rated health. The lower the social class, the lower the self-reported physical and mental health. Health self-management partially mediated the relationship between social class and self-rated health. That is, the health self-management ability of the lower class, such as access to healthy and nutritious food and evaluate their own health status, is worse than that of the higher class, which leads to physical and mental health inequality between the high and the low classes. Conclusion Health self-management mediated the relationship between social class and health. Promoting health self-management abilities are conducive to improving both physical and mental health.
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- 2021
21. A handy method to remove bacterial contamination from fungal cultures
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Haiping Qiu, Yanli Wang, Xiao-Xiao Shi, Zhen Zhang, Guochang Sun, and Jiaoyu Wang
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0301 basic medicine ,Culture plates ,Microbiological Techniques ,Agrobacteria ,Antibiotics ,Cell Culture Techniques ,Social Sciences ,Artificial Gene Amplification and Extension ,Plant Science ,Pathology and Laboratory Medicine ,Polymerase Chain Reaction ,Plant Microbiology ,Medicine and Health Sciences ,Psychology ,Fungal Pathogens ,Multidisciplinary ,biology ,Animal Behavior ,Chemistry ,Antimicrobials ,Plant Fungal Pathogens ,Drugs ,Eukaryota ,Contamination ,Solid medium ,Medical Microbiology ,Animal Sociality ,Fungal strain ,Medicine ,Pathogens ,Research Article ,medicine.drug_class ,Science ,030106 microbiology ,Plant Pathogens ,Hyphae ,Mycology ,Research and Analysis Methods ,Microbiology ,Agrobacterium Tumefaciens ,03 medical and health sciences ,Microbial Control ,medicine ,Molecular Biology Techniques ,Microbial Pathogens ,Molecular Biology ,Pharmacology ,Contamination control ,Behavior ,Bacteria ,Organisms ,Fungi ,Biology and Life Sciences ,Correction ,Plant Pathology ,biology.organism_classification ,Culture Media ,030104 developmental biology ,Feasibility Studies ,Zoology ,Fungal hyphae - Abstract
Contamination control and removal are very important technical aspects of microbiological research. Bacterial contamination is very common in fungal cultures. Currently, the commonly used approach for inhibiting bacteria is antibiotic treatment; however, there are drawbacks to using antibiotics, including incomplete removal, limited antibacterial spectra, tendency toward recontamination, effects to fungal strains, and potential risks to the environment. Therefore, in the present work, we developed a new method for bacterial removal from fungi cultured on solid medium, the Cabin-Sequestering (CS) method, based on the different culture characteristics between fungi and bacteria. First, 3–5 mm round or square holes (the “cabin”) are excavated on a solid medium plate. The fungal strain containing possible bacterial contamination is inoculated into the cabin. The cabin is then covered with a sterilized coverslip, followed by incubation at the appropriate temperature. After 7–10 days of culturing, fungal hyphae grow out along the edge of the coverslip; however, the contaminating bacteria cannot pass through the space formed between the medium and the coverslip and, thus, remain in the cabin. The newly grown fungal hyphae around the coverslip are re-inoculated into fresh culture plates, where they form bacteria-free fungal colonies. The CS method is easy handling, with a short experimental cycle and rare recontamination. When necessary, it can also be used in combination with antibiotics in bacterial removal operations.
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- 2019
22. Intraductal Radiofrequency Ablation Followed by Locoregional Tumor Treatments for Treating Occluded Biliary Stents in Non-Resectable Malignant Biliary Obstruction: A Single-Institution Experience
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Pengfei Chen, Jianzhuang Ren, Xuhua Duan, Jianhao Zhang, Yanli Wang, Xinwei Han, Teng-Fei Li, and Kai Zhang
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Male ,medicine.medical_specialty ,Radiofrequency ablation ,medicine.medical_treatment ,lcsh:Medicine ,Catheter ablation ,Bile Duct Neoplasm ,Cholestasis, Intrahepatic ,Constriction, Pathologic ,law.invention ,law ,medicine ,Humans ,Gallbladder cancer ,lcsh:Science ,Survival rate ,Aged ,Retrospective Studies ,Multidisciplinary ,business.industry ,Gallbladder ,lcsh:R ,Stent ,Bilirubin ,Middle Aged ,medicine.disease ,Survival Analysis ,Surgery ,Survival Rate ,Jaundice, Obstructive ,medicine.anatomical_structure ,surgical procedures, operative ,Bile Ducts, Intrahepatic ,Treatment Outcome ,Bile Duct Neoplasms ,Hepatocellular carcinoma ,Catheter Ablation ,lcsh:Q ,Female ,Stents ,Radiology ,business ,Follow-Up Studies ,Research Article - Abstract
Objectives To determine the safety and feasibility of intraductal radiofrequency ablation (RFA) followed by locoregional tumor treatments in patients with non-resectable malignant biliary obstruction and stent re-occlusion. Methods Fourteen patients with malignant biliary obstruction and blocked metal stents were studied retrospectively. All had intraductal RFA followed by locoregional tumor treatments and were monitored clinically and radiologically. The practicality, safety, postoperative complications, jaundice remission, stent patency and survival time were analyzed. Results Combination treatment was successful for all patients. There were no severe complications during RFA or local treatments. All patients had stent patency restored, with a decline in serum bilirubin. Three patients had recurrent jaundice by 195, 237 and 357 days; two patients underwent repeat intraductal RFA; and one required an internal-external biliary drain. The average stent patency time was 234 days (range 187-544 days). With a median follow-up of 384 days (range 187-544 days), six patients were alive, while eight had died. There was no mortality at 30 days. The 3, 6, 12 and 18 month survival rates were 100%, 100%, 64.3% and 42.9%, respectively. Conclusion Intraductal RFA followed by locoregional tumor treatments for occluded metal stents is safe and practically feasible and potential increase stent patency and survival times.
- Published
- 2015
23. One of Three Pex11 Family Members Is Required for Peroxisomal Proliferation and Full Virulence of the Rice Blast Fungus Magnaporthe oryzae
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Yanli Wang, Yuan Fang, Zhen Zhang, Guochang Sun, Hua Jiang, Rong Yao Chai, Ling Li, Haiping Qiu, Xueqin Mao, Dongmei Li, and Jiaoyu Wang
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Magnaporthe ,Molecular Sequence Data ,Mutant ,Virulence ,lcsh:Medicine ,Biology ,Microbiology ,Fungal Proteins ,Woronin body ,Cell Wall ,Peroxisomes ,Amino Acid Sequence ,lcsh:Science ,Phylogeny ,Plant Diseases ,Fungal protein ,Appressorium ,Multidisciplinary ,Sequence Homology, Amino Acid ,lcsh:R ,Fungal genetics ,Oryza ,Peroxisome ,biology.organism_classification ,lcsh:Q ,Research Article - Abstract
Peroxisomes play important roles in metabolisms of eukaryotes and infection of plant fungal pathogens. These organelles proliferate by de novo formation or division in response to environmental stimulation. Although the assembly of peroxisomes was documented in fungal pathogens, their division and its relationship to pathogenicity remain obscure. In present work, we analyzed the roles of three Pex11 family members in peroxisomal division and pathogenicity of the rice blast fungus Magnaporthe oryzae. Deletion of MoPEX11A led to fewer but enlarged peroxisomes, and impaired the separation of Woronin bodies from peroxisomes, while deletion of MoPEX11B or MoPEX11C put no evident impacts to peroxisomal profiles. MoPEX11A mutant exhibited typical peroxisome related defects, delayed conidial germination and appressoria formation, and decreased appressorial turgor and host penetration. As a result, the virulence of MoPEX11A mutant was greatly reduced. Deletion of MoPEX11B and MoPEX11C did not alter the virulence of the fungus. Further, double or triple deletions of the three genes were unable to enhance the virulence decrease in MoPEX11A mutant. Our data indicated that MoPEX11A is the main factor modulating peroxisomal division and is required for full virulence of the fungus.
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- 2015
24. Urotensin II promotes atherosclerosis in cholesterol-fed rabbits
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Min Wu, Daxing Cheng, Yulong Chen, Enqi Liu, Ninghong Zhu, Huadong Zheng, Yanli Wang, Yafeng Li, Yandong Li, Liang Bai, Yan Lin, Sihai Zhao, and Jianglin Fan
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Aortic arch ,medicine.medical_specialty ,Clinical Research Design ,Urotensins ,Cardiology ,Aortic Diseases ,lcsh:Medicine ,Research and Analysis Methods ,Vascular Medicine ,Umbilical vein ,Cholesterol, Dietary ,chemistry.chemical_compound ,Model Organisms ,Internal medicine ,medicine.artery ,Blood plasma ,medicine ,Medicine and Health Sciences ,Thoracic aorta ,Animals ,Animal Models of Disease ,lcsh:Science ,Aorta ,Multidisciplinary ,Cholesterol ,lcsh:R ,Abdominal aorta ,Body Weight ,Biology and Life Sciences ,Animal Models ,Atherosclerosis ,Lipids ,Endocrinology ,chemistry ,Research Design ,Cardiovascular Anatomy ,lcsh:Q ,Rabbits ,Anatomy ,Urotensin-II ,Research Article - Abstract
Urotensin II (UII) is a vasoactive peptide composed of 11 amino acids that has been implicated to contribute to the development of cardiovascular disease. The purpose of this study was to investigate whether UII affects the development of atherosclerosis in cholesterol-fed rabbits. UII was infused for 16 weeks through an osmotic mini-pump into male Japanese White rabbits fed on a high-cholesterol diet. Plasma lipids and body weight were measured every 4 weeks. Aortic atherosclerotic lesions along with cellular components, collagen fibers, matrix metalloproteinase-1 and -9 were examined. Moreover, vulnerability index of atherosclerotic plaques was evaluated. UII infusion significantly increased atherosclerotic lesions within the entire aorta by 21% over the control (P = 0.013). Atherosclerotic lesions were increased by 24% in the aortic arch (P = 0.005), 11% in the thoracic aorta (P = 0.054) and 18% in the abdominal aorta (P = 0.035). These increases occurred without changes in plasma levels of total cholesterol, low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, triglycerides or body weight. Immunohistochemical staining revealed that macrophages and matrix metalloproteinase-9 were significantly enhanced by 2.2-fold and 1.6-fold in UII group. In vitro studies demonstrated that UII up-regulated the expression of vascular cell adhesion protein-1 and intercellular adhesion molecule-1 in human umbilical vein endothelial cells, which was inhibited by the UII receptor antagonist urantide. In conclusion, our results showed that UII promotes the development of atherosclerotic lesions and destabilizes atherosclerotic plaques in cholesterol-fed rabbits.
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- 2013
25. PTS1 Peroxisomal Import Pathway Plays Shared and Distinct Roles to PTS2 Pathway in Development and Pathogenicity of Magnaporthe oryzae
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Zhen Zhang, Haiping Qiu, Guochang Sun, Rongyao Chai, Fu-Cheng Lin, Xueqin Mao, Yanli Wang, Xinfa Du, Jiaoyu Wang, Hua Jiang, and Ling Li
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Magnaporthe ,Peroxisome-Targeting Signal 1 Receptor ,lcsh:Medicine ,Receptors, Cytoplasmic and Nuclear ,Pathogenesis ,Plant Science ,Plant Microbiology ,Cloning, Molecular ,lcsh:Science ,Peroxisomal Targeting Signal 2 Receptor ,Fungal protein ,Multidisciplinary ,biology ,Fungal genetics ,Agriculture ,Genomics ,Peroxisome ,Spores, Fungal ,Cell biology ,Functional Genomics ,Host-Pathogen Interaction ,Blotting, Southern ,Protein Transport ,Phenotype ,Biochemistry ,Agrochemicals ,Research Article ,Signal Transduction ,Molecular Sequence Data ,Plant Pathogens ,Cereals ,Crops ,Microbiology ,Fungal Proteins ,Microscopy, Electron, Transmission ,Peroxisomes ,Amino Acid Sequence ,Biology ,Microbial Pathogens ,Plant Diseases ,Sequence Homology, Amino Acid ,Peroxisomal matrix ,Peroxisomal Targeting Signal 1 ,lcsh:R ,Crop Diseases ,Oryza ,Hydrogen Peroxide ,Plant Pathology ,biology.organism_classification ,lcsh:Q ,Rice - Abstract
Peroxisomes participate in various important metabolisms and are required in pathogenicity of fungal plant pathogens. Peroxisomal matrix proteins are imported from cytoplasm into peroxisomes through peroxisomal targeting signal 1 (PTS1) or peroxisomal targeting signal 2 (PTS2) import pathway. PEX5 and PEX7 genes participate in the two pathways respectively. The involvement of PEX7 mediated PTS2 import pathway in fungal pathogenicity has been documented, while that of PTS1 remains unclear. Through null mutant analysis of MoPEX5, the PEX5 homolog in Magnaporthe oryzae, we report the crucial roles of PTS1 pathway in the development and host infection in the rice blast fungus, and compared with those of PTS2. We found that MoPEX5 disruption specifically blocked the PTS1 pathway. Δmopex5 was unable to use lipids as sole carbon source and lost pathogenicity completely. Similar as Δmopex7, Δmopex5 exhibited significant reduction in lipid utilization and mobilization, appressorial turgor genesis and H(2)O(2) resistance. Additionally, Δmopex5 presented some distinct defects which were undetected in Δmopex7 in vegetative growth, conidial morphogenesis, appressorial morphogenesis and melanization. The results indicated that the PTS1 peroxisomal import pathway, in addition to PTS2, is required for fungal development and pathogenicity of the rice blast fungus, and also, as a main peroxisomal import pathway, played a more predominant role than PTS2.
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- 2013
26. Cytokine and Chemokine Levels in Patients with Severe Fever with Thrombocytopenia Syndrome Virus
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Pei Liu, Yuzhong Zhang, Shujun Zhang, Wenqing Yao, Baocheng Deng, Wen Wang, Ying Wen, Yu Wang, Ying Zhou, Yuncheng Wang, Chengbo Li, Yanli Wang, Zhen Shao, Wei Cui, Yingzhi Geng, Yitong Zhao, Donglei Wang, and Qiuhong Gu
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Male ,Phlebovirus ,Chemokine ,Viral Diseases ,Anatomy and Physiology ,medicine.medical_treatment ,lcsh:Medicine ,Pathogenesis ,Immune Physiology ,Zoonoses ,Prospective Studies ,lcsh:Science ,Immune Response ,Aged, 80 and over ,Multidisciplinary ,biology ,SFTS virus ,Middle Aged ,Cytokine ,Infectious Diseases ,Observational Studies ,Cytokines ,Medicine ,Female ,Chemokines ,Research Article ,Adult ,China ,Adolescent ,Clinical Research Design ,Immunology ,Bunyaviridae Infections ,Microbiology ,Young Adult ,Virology ,medicine ,Humans ,Biology ,Aged ,Survey Research ,business.industry ,lcsh:R ,Case-control study ,Immunity ,Tropical Diseases (Non-Neglected) ,Molecular Development ,medicine.disease ,biology.organism_classification ,Severe fever with thrombocytopenia syndrome ,Case-Control Studies ,Immune System ,biology.protein ,Clinical Immunology ,lcsh:Q ,business ,Severe fever with thrombocytopenia syndrome virus ,Developmental Biology - Abstract
BACKGROUND: Severe fever with thrombocytopenia syndrome virus (SFTSV), which can cause hemorrhagic fever-like illness, is a newly discovered bunyavirus in China. The pathogenesis of SFTSV infection is poorly understood. However, it has been suggested that immune mechanisms, including cytokines and chemokines, play an important role in disease pathogenesis. In the present study, we investigated host cytokine and chemokine profiles in serum samples of patients with SFTSV infection from Northeast China and explored a possible correlation between cytokine levels and disease severity. METHODS AND PRINCIPAL FINDINGS: Acute phase serum samples from 40 patients, diagnosed with SFTSV infection were included. Patients were divided into two groups--severe or non-severe--based on disease severity. Levels of tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β, interleukin-6, interferon (IFN)-γ, IFN- γ-induced protein (IP)-10 and RANTES were measured in the serum samples with commercial ELISAs. Statistical analysis showed that increases in TNF-α, IP-10 and IFN-γ were associated with disease severity. CONCLUSIONS: We suggest that a cytokine-mediated inflammatory response, characterized by cytokine and chemokine production imbalance, might be in part responsible for the disease progression of patients with SFTSV infection.
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- 2012
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27. Inward Rectifier K+ Currents Are Regulated by CaMKII in Endothelial Cells of Primarily Cultured Bovine Pulmonary Arteries
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Weiwei Zhong, Xiufeng Yu, Lei Yu, Yanli Wang, Daling Zhu, Lihui Qu, Ping Lu, Qianlong Zhang, Xin Jin, Chun Jiang, Ningren Cui, and Xiaodong Zheng
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medicine.medical_specialty ,Patch-Clamp Techniques ,Calmodulin ,Endothelium ,Voltage clamp ,Blotting, Western ,lcsh:Medicine ,Pulmonary Artery ,Biology ,Immunoenzyme Techniques ,chemistry.chemical_compound ,Internal medicine ,Ca2+/calmodulin-dependent protein kinase ,medicine ,Animals ,Patch clamp ,Phosphorylation ,Potassium Channels, Inwardly Rectifying ,lcsh:Science ,Cells, Cultured ,Multidisciplinary ,Inward-rectifier potassium ion channel ,lcsh:R ,Potassium channel ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Pinacidil ,Potassium ,cardiovascular system ,biology.protein ,Biophysics ,Cattle ,lcsh:Q ,Endothelium, Vascular ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,Research Article - Abstract
Endothelium lines the interior surface of vascular walls and regulates vascular tones. The endothelial cells sense and respond to chemical and mechanical stimuli in the circulation, and couple the stimulus signals to vascular smooth muscles, in which inward rectifier K+ currents (Kir) play an important role. Here we applied several complementary strategies to determine the Kir subunit in primarily cultured pulmonary arterial endothelial cells (PAECs) that was regulated by the Ca2+/calmodulin (CaM)-dependent protein kinase II (CaMKII). In whole-cell voltage clamp, the Kir currents were sensitive to micromolar concentrations of extracellular Ba2+. In excised inside-out patches, an inward rectifier K+ current was observed with single-channel conductance 32.43 ± 0.45 pS and Popen 0.27 ± 0.04, which were consistent with known unitary conductance of Kir 2.1. RT-PCR and western blot results showed that expression of Kir 2.1 was significantly stronger than that of other subtypes in PAECs. Pharmacological analysis of the Kir currents demonstrated that insensitivity to intracellular ATP, pinacidil, glibenclamide, pH, GDP-β-S and choleratoxin suggested that currents weren't determined by KATP, Kir2.3, Kir2.4 and Kir3.x. The currents were strongly suppressed by exposure to CaMKII inhibitor W-7 and KN-62. The expression of Kir2.1 was inhibited by knocking down CaMKII. Consistently, vasodilation was suppressed by Ba2+, W-7 and KN-62 in isolated and perfused pulmonary arterial rings. These results suggest that the PAECs express an inward rectifier K+ current that is carried dominantly by Kir2.1, and this K+ channel appears to be targeted by CaMKII-dependent intracellular signaling systems.
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- 2015
28. An S-(Hydroxymethyl)Glutathione Dehydrogenase Is Involved in Conidiation and Full Virulence in the Rice Blast Fungus Magnaporthe oryzae
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Yanli Wang, Guochang Sun, Hua Jiang, Fengquan Liu, Jiaoyu Wang, Rongyao Chai, Xueqin Mao, Zhen Zhang, and Haiping Qiu
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Magnaporthe ,Science ,Mutant ,Gene Expression ,Conidiation ,Virulence ,Nitric Oxide ,chemistry.chemical_compound ,Plant defense against herbivory ,Plant Diseases ,Appressorium ,S-Nitrosothiols ,Multidisciplinary ,biology ,Genetic Complementation Test ,Wild type ,food and beverages ,Oryza ,Hydrogen Peroxide ,Glutathione ,biology.organism_classification ,Oxidative Stress ,Biochemistry ,chemistry ,Mutation ,Medicine ,Genes, Lethal ,Oxidoreductases ,Research Article - Abstract
Magnaporthe oryzae is a hemibiotrophic fungal pathogen that causes rice blast disease. A compatible interaction requires overcoming plant defense responses to initiate colonization during the early infection process. Nitric oxide (NO) plays important roles in defense responses during host-pathogen interactions. Microbes generally protect themselves against NO-induced damage by using enzymes. Here, we characterized an S-(hydroxymethyl)- glutathione dehydrogenase gene in M. oryzae, MoSFA1, the homologs of which are involved in NO metabolism by specifically catalyzing the reduction of S-nitrosoglutathione (GSNO) in yeasts and plants. As expected from the activities of S-(hydroxymethyl)glutathione dehydrogenase in formaldehyde detoxification and GSNO reduction, MoSFA1 deletion mutants were lethal in formaldehyde containing medium, sensitive to exogenous NO and exhibited a higher level of S-nitrosothiols (SNOs) than that of the wild type. Notably, the mutants showed severe reduction of conidiation and appressoria turgor pressure, as well as significantly attenuated the virulence on rice cultivar CO-39. However, the virulence of MoSFA1 deletion mutants on wounded rice leaf was not affected. An infection assay on barley leaf further revealed that MoSFA1 deletion mutants exhibited a lower infection rate, and growth of infectious hyphae of the mutants was retarded not only in primary infected cells but also in expansion from cell to cell. Furthermore, barley leaf cell infected by MoSFA1 deletion mutants exhibited a stronger accumulation of H2O2 at 24 and 36 hpi. MoSFA1 deletion mutants displayed hypersensitivity to different oxidants, reduced activities of superoxide dismutases and peroxidases, and lower glutathione content in cells, compared with the wild type. These results imply that MoSFA1-mediated NO metabolism is important in redox homeostasis in response to development and host infection of M. oryzae. Taken together, this work identifies that MoSFA1 is required for conidiation and contributes to virulence in the penetration and biotrophic phases in M. oryzae.
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- 2015
29. Synergistic Association between Two Alcohol Metabolism Relevant Genes and Coronary Artery Disease among Chinese Hypertensive Patients
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Jun Liu, Xiaohong Yu, Hongye Zhao, Fengxia Du, Yuefei Wang, Yu Xiao, Wenquan Niu, Changzhu Lu, Xue Li, Bin Wang, and Yanli Wang
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Male ,China ,Genotype ,Cardiology ,lcsh:Medicine ,Polygenic disease ,Coronary Artery Disease ,Bioinformatics ,Polymorphism, Single Nucleotide ,Aldehyde Dehydrogenase 1 Family ,Coronary artery disease ,Asian People ,Gene Frequency ,Medicine and Health Sciences ,Humans ,Coronary Heart Disease ,Medicine ,Genetic Predisposition to Disease ,cardiovascular diseases ,Allele ,Ethanol metabolism ,lcsh:Science ,Gene ,Alleles ,Aged ,ALDH2 ,Clinical Genetics ,Evolutionary Biology ,Multidisciplinary ,Population Biology ,business.industry ,Aldehyde Dehydrogenase, Mitochondrial ,lcsh:R ,Biology and Life Sciences ,ADH1B ,Aldehyde Dehydrogenase ,Middle Aged ,medicine.disease ,Blood pressure ,Haplotypes ,Cardiovascular Diseases ,Case-Control Studies ,Hypertension ,lcsh:Q ,Female ,business ,Population Genetics ,Research Article - Abstract
Objective Coronary artery disease (CAD) is a multifactorial and polygenic disease. The aim of this study was to examine the association between six polymorphisms of four alcohol metabolism relevant genes (ADH1B, ADH1C, ALDH1b1, ALDH2) and the risk of CAD in Han Chinese. Methods and Results This was a hospital-based case-control study involving 1365 hypertensive patients. All study subjects were angiographically confirmed. Genotypes were determined with ligase detection reaction method. There was no observable deviation from the Hardy-Weinberg equilibrium for six examined polymorphisms in controls. The genotype and allele distributions of ALDH1b1 rs2073478 and ALDH2 rs671 polymorphisms differed significantly between the two groups (P≤0.005), even after the Bonferroni correction. The most common allele combination was A-C-C-G-C-G (alleles in order of rs1229984, rs1693482, rs2228093, rs2073478, rs886205, rs671) and its frequency was slightly higher in controls than in CAD patients (P = 0.067). After assigning the most common allele combination as a reference, allele combination A-C-C-T-C-A, which simultaneously possessed the risk alleles of rs2073478 and rs671 polymorphisms, was associated with a 1.80-fold greater risk of CAD. Further, a two-locus model including rs2073478 and rs671 that had a maximal testing accuracy of 0.598 and a cross-validation consistency of 10 (P = 0.008) was deemed as the overall best MDR model, which was further validated by classical Logistic regression model. Conclusion Our findings provide clear evidence for both individual and interactive associations of ALDH1b1 and ALDH2 genes with the development of CAD in Han Chinese.
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- 2014
30. The Contributory Role of Angiotensin Receptor-Like 1 Gene Multiple Polymorphisms in Hypertension among Northeastern Han Chinese
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Yuefei Wang, Bin Wang, Changzhu Lu, Ruoshan Liu, Wenquan Niu, Nan Jia, Yu Xiao, Yanli Wang, and Hongye Zhao
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Male ,China ,medicine.medical_specialty ,Clinical Research Design ,Epidemiology ,lcsh:Medicine ,Biology ,Cardiovascular ,Bioinformatics ,Polymorphism, Single Nucleotide ,Receptors, G-Protein-Coupled ,Asian People ,Gene Frequency ,Internal medicine ,Genetic model ,Genetics ,medicine ,Humans ,Genetic Predisposition to Disease ,Allele ,lcsh:Science ,Allele frequency ,Genetic Association Studies ,Aged ,Clinical Genetics ,Apelin Receptors ,Multidisciplinary ,Models, Genetic ,Multifactor dimensionality reduction ,lcsh:R ,Haplotype ,Confounding ,Case-control study ,Human Genetics ,Middle Aged ,Confidence interval ,Endocrinology ,Haplotypes ,Case-Control Studies ,Genetic Epidemiology ,Genetics of Disease ,Hypertension ,Genetic Polymorphism ,Medicine ,Female ,lcsh:Q ,Population Genetics ,Research Article - Abstract
Background and Objective Via direct sequencing, we have recently identified six common polymorphisms in angiotensin receptor-like 1 (AGTRL1) gene, and found only two polymorphisms were significantly associated with hypertension in a family-based analysis on 1,015 southern Han Chinese. Extending our previous work and considering the ubiquity of epistasis in determining disease susceptibility, we, in this study, sought to explore the potential interaction of AGTRL1 gene six polymorphisms with hypertension in a large northeastern Han Chinese population. Methods and Results This was a case-control study involving 1,009 sporadic hypertensive patients and 756 normotensive controls. Data were analyzed by Haplo.Stats and multifactor dimensionality reduction (MDR) softwares. There were no deviations from Hardy-Weinberg equilibrium for all polymorphisms. The genotypes and alleles of rs7119675 and rs11544374 differed significantly between the two groups (P
- Published
- 2014
31. MoPex19, which Is Essential for Maintenance of Peroxisomal Structure and Woronin Bodies, Is Required for Metabolism and Development in the Rice Blast Fungus
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Guochang Sun, Rongyao Chai, Zhen Zhang, Maoxin Liu, Ling Li, Xueqin Mao, Jiaoyu Wang, Haiping Qiu, Fengquan Liu, Hua Jiang, and Yanli Wang
- Subjects
Cytoplasm ,Magnaporthe ,Molecular Sequence Data ,Plant Pathogens ,Hyphae ,lcsh:Medicine ,Pathogenesis ,Plant Science ,Microbiology ,Fungal Proteins ,Plant Microbiology ,Woronin body ,Cell Wall ,Sequence Homology, Nucleic Acid ,Molecular Cell Biology ,Peroxisomal disorder ,Peroxisomes ,medicine ,Amino Acid Sequence ,lcsh:Science ,Biology ,Microbial Pathogens ,Sequence Deletion ,Fungal protein ,Multidisciplinary ,biology ,Peroxisomal matrix ,lcsh:R ,Microbial Mutation ,Fungal genetics ,Membrane Proteins ,Oryza ,Plant Pathology ,Spores, Fungal ,Peroxisome ,medicine.disease ,biology.organism_classification ,Cellular Structures ,Host-Pathogen Interaction ,Subcellular Organelles ,Biochemistry ,Membrane protein ,lcsh:Q ,Research Article - Abstract
Peroxisomes are present ubiquitously and make important contributions to cellular metabolism in eukaryotes. They play crucial roles in pathogenicity of plant fungal pathogens. The peroxisomal matrix proteins and peroxisomal membrane proteins (PMPs) are synthesized in the cytosol and imported post-translationally. Although the peroxisomal import machineries are generally conserved, some species-specific features were found in different types of organisms. In phytopathogenic fungi, the pathways of the matrix proteins have been elucidated, while the import machinery of PMPs remains obscure. Here, we report that MoPEX19, an ortholog of ScPEX19, was required for PMPs import and peroxisomal maintenance, and played crucial roles in metabolism and pathogenicity of the rice blast fungus Magnaporthe oryzae. MoPEX19 was expressed in a low level and Mopex19p was distributed in the cytoplasm and newly formed peroxisomes. MoPEX19 deletion led to mislocalization of peroxisomal membrane proteins (PMPs), as well peroxisomal matrix proteins. Peroxisomal structures were totally absent in Δmopex19 mutants and woronin bodies also vanished. Δmopex19 exhibited metabolic deficiency typical in peroxisomal disorders and also abnormality in glyoxylate cycle which was undetected in the known mopex mutants. The Δmopex19 mutants performed multiple disorders in fungal development and pathogenicity-related morphogenesis, and lost completely the pathogenicity on its hosts. These data demonstrate that MoPEX19 plays crucial roles in maintenance of peroxisomal and peroxisome-derived structures and makes more contributions to fungal development and pathogenicity than the known MoPEX genes in the rice blast fungus.
- Published
- 2014
32. MiR-146a Regulates SOD2 Expression in H2O2 Stimulated PC12 Cells
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Ke Lv, Yanli Wang, Yinghui Li, Dingsheng Zhao, Lina Qu, Tingmei Wang, Guohua Ji, and Hailong Chen
- Subjects
Cell Survival ,Molecular Sequence Data ,SOD2 ,lcsh:Medicine ,Gene Expression ,Biology ,PC12 Cells ,Biochemistry ,Gene Expression Regulation, Enzymologic ,Stress Signaling Cascade ,Molecular Genetics ,Superoxide dismutase ,RNA interference ,Molecular cell biology ,microRNA ,Gene expression ,Genetics ,Animals ,Humans ,RNA, Antisense ,Gene Regulation ,RNA, Messenger ,Viability assay ,lcsh:Science ,skin and connective tissue diseases ,3' Untranslated Regions ,Cellular Stress Responses ,Regulation of gene expression ,Multidisciplinary ,Base Sequence ,Superoxide Dismutase ,Three prime untranslated region ,lcsh:R ,Computational Biology ,Hydrogen Peroxide ,Molecular biology ,Signaling Cascades ,Rats ,MicroRNAs ,Regulatory sequence ,cardiovascular system ,biology.protein ,lcsh:Q ,Epigenetics ,Research Article ,Signal Transduction - Abstract
SOD2 (superoxide dismutase 2) is one of the endogenous antioxidant enzymes that protect against reactive oxygen species. While explorations of SOD2 expression regulation are mainly focused on transcriptional and post-translational activation, there are few reports about the post-transcriptional regulation of SOD2. MicroRNAs (miRNAs) are 21nt-25nt (nucleotide) small noncoding RNAs that have emerged as indispensable regulators of gene expression. Here we show that miR-146a, a widely expressed miRNA, is up-regulated by H2O2-induced stress. By sequence analysis we found a binding site for miR-146a in the sod2 mRNA 3'UTR, and a luciferase reporter assay confirmed that miR-146a can interact with this sod2 regulatory region. Our results further show that miR-146a could down-regulate the SOD2 protein expression, and antisense-miR-146a could reverse the decrease of both the SOD2 level and cell viability in H2O2 treated PC12 cells. In conclusion, here we have identified a novel function of miR-146a in the post-transcriptional regulation of SOD2 expression.
- Published
- 2013
33. Differential Expression of In Vivo and In Vitro Protein Profile of Outer Membrane of Acidovorax avenae Subsp. avenae.
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Ibrahim, Muhammad, Yu Shi, Hui Qiu, Bin Li, Jabeen, Amara, Liping Li, He Liu, Kube, Michael, Guanlin Xie, Yanli Wang, and Guochang Sun
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GLUCAGON receptors ,AMINO acids ,PATHOLOGICAL physiology ,HYPERGLYCEMIA ,TREATMENT of diabetes ,HYPOGLYCEMIA - Abstract
Outer membrane (OM) proteins play a significant role in bacterial pathogenesis. In this work, we examined and compared the expression of the OM proteins of the rice pathogen Acidovorax avenae subsp. avenae strain RS-1, a Gram-negative bacterium, both in an in vitro culture medium and in vivo rice plants. Global proteomic profiling of A. avenae subsp. avenae strain RS-1 comparing in vivo and in vitro conditions revealed the differential expression of proteins affecting the survival and pathogenicity of the rice pathogen in host plants. The shotgun proteomics analysis of OM proteins resulted in the identification of 97 proteins in vitro and 62 proteins in vivo by mass spectrometry. Among these OM proteins, there is a high number of porins, TonB-dependent receptors, lipoproteins of the NodT family, ABC transporters, flagellins, and proteins of unknown function expressed under both conditions. However, the major proteins such as phospholipase and OmpA domain containing proteins were expressed in vitro, while the proteins such as the surface anchored protein F, ATP- dependent Clp protease, OmpA and MotB domain containing proteins were expressed in vivo. This may indicate that these in vivo OM proteins have roles in the pathogenicity of A. avenae subsp. avenae strain RS-1. In addition, the LC-MS/MS identification of OmpA and MotB validated the in silico prediction of the existence of Type VI secretion system core components. To the best of our knowledge, this is the first study to reveal the in vitro and in vivo protein profiles, in combination with LC-MS/MS mass spectra, in silico OM proteome and in silico genome wide analysis, of pathogenicity or plant host required proteins of a plant pathogenic bacterium. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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