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14. Transcriptome/Degradome-Wide Identification of R. glutinosa miRNAs and Their Targets: The Role of miRNA Activity in the Replanting Disease.

Author

Li, Ming Jie, Yang, Yan Hui, Chen, Xin Jian, Wang, Feng Qing, Lin, Wen Xiong, Yi, Yan Jie, Zeng, Lei, Yang, Shuo Ye, and Zhang, Zhong Yi

Subjects
GENETIC transcription, MICRORNA, REPLANT diseases, CHINESE medicine, CULTIVATED plants, PLANT regulators, PLANT development
Abstract

Rehmannia glutinosa, a traditional Chinese medicine herb, is unable to grow normally in a soil where the same species has recently been cultivated. The biological basis of this so called “replanting disease” is unknown, but it may involve the action of microRNAs (miRNAs), which are known to be important regulators of plant growth and development. High throughput Solexa/Illumina sequencing was used to generate a transcript library of the R. glutinosa transcriptome and degradome in order to identify possible miRNAs and their targets implicated in the replanting disease. A total of 87,665 unigenes and 589 miRNA families (17 of which have not been identified in plants to date) was identified from the libraries made from a first year (FP) and a second year (SP) crop. A comparison between the FP and SP miRNAs showed that the abundance of eight of the novel and 295 of the known miRNA families differed between the FP and SP plants. Sequencing of the degradome sampled from FP and SP plants led to the identification of 165 transcript targets of 85 of the differentially abundant miRNA families. The interaction of some of these miRNAs with their target(s) is likely to form an important part of the molecular basis of the replanting disease of R. glutinosa. [ABSTRACT FROM AUTHOR]

Published
2013
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