1. Nanoparticle-Encapsulated Chlorhexidine against Oral Bacterial Biofilms.
- Author
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Seneviratne, Chaminda Jayampath, Leung, Ken Cham-Fai, Wong, Chi-Hin, Lee, Siu-Fung, Li, Xuan, Leung, Ping Chung, Lau, Clara Bik San, Wat, Elaine, and Jin, Lijian
- Subjects
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CHLORHEXIDINE , *BIOFILMS , *ANTI-infective agents , *DENTISTRY , *MESOPOROUS materials , *SILICA nanoparticles , *ULTRAVIOLET spectroscopy - Abstract
Background: Chlorhexidine (CHX) is a widely used antimicrobial agent in dentistry. Herein, we report the synthesis of a novel mesoporous silica nanoparticle-encapsulated pure CHX (Nano-CHX), and its mechanical profile and antimicrobial properties against oral biofilms. Methodology/Principal Findings: The release of CHX from the Nano-CHX was characterized by UV/visible absorption spectroscopy. The antimicrobial properties of Nano-CHX were evaluated in both planktonic and biofilm modes of representative oral pathogenic bacteria. The Nano-CHX demonstrated potent antibacterial effects on planktonic bacteria and mono-species biofilms at the concentrations of 50–200 µg/mL against Streptococcus mutans, Streptococcus sobrinus, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans and Enterococccus faecalis. Moreover, Nano-CHX effectively suppressed multi-species biofilms such as S. mutans, F. nucleatum, A. actinomycetemcomitans and Porphyromonas gingivalis up to 72 h. Conclusions/Significance: This pioneering study demonstrates the potent antibacterial effects of the Nano-CHX on oral biofilms, and it may be developed as a novel and promising anti-biofilm agent for clinical use. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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