Search

Your search keyword '"Wilkinson, David"' showing total 14 results
Start Over Author "Wilkinson, David" Journal plos one
14 results on '"Wilkinson, David"'

1. Vector competence of Aedes albopictus field populations from Reunion Island exposed to local epidemic dengue viruses.

Author

Hafsia, Sarah, Barbar, Tatiana, Alout, Haoues, Baudino, Fiona, Lebon, Cyrille, Gomard, Yann, Wilkinson, David A., Fourié, Toscane, Mavingui, Patrick, and Atyame, Célestine

Subjects
AEDES albopictus, DENGUE viruses, GENETIC vectors, VIRAL load, WOLBACHIA
Abstract

Dengue virus (DENV) is the most prevalent mosquito-borne Flavivirus that affects humans worldwide. Aedes albopictus, which is naturally infected with the bacteria Wolbachia, is considered to be a secondary vector of DENV. However, it was responsible for a recent DENV outbreak of unprecedented magnitude in Reunion Island, a French island in the South West Indian Ocean. Moreover, the distribution of the cases during this epidemic showed a spatially heterogeneous pattern across the island, leading to questions about the differential vector competence of mosquito populations from different geographic areas. The aim of this study was to gain a better understanding of the vector competence of the Ae. albopictus populations from Reunion Island for local DENV epidemic strains, while considering their infection by Wolbachia. Experimental infections were conducted using ten populations of Ae. albopictus sampled across Reunion Island and exposed to three DENV strains: one strain of DENV serotype 1 (DENV-1) and two strains of DENV serotype 2 (DENV-2). We analyzed three vector competence parameters including infection rate, dissemination efficiency and transmission efficiency, at different days post-exposition (dpe). We also assessed whether there was a correlation between the density of Wolbachia and viral load/vector competence parameters. Our results show that the Ae. albopictus populations tested were not able to transmit the two DENV-2 strains, while transmission efficiencies up to 40.79% were observed for the DENV-1 strain, probably due to difference in viral titres. Statistical analyses showed that the parameters mosquito population, generation, dpe and area of sampling significantly affect the transmission efficiencies of DENV-1. Although the density of Wolbachia varied according to mosquito population, no significant correlation was found between Wolbachia density and either viral load or vector competence parameters for DENV-1. Our results highlight the importance of using natural mosquito populations for a better understanding of transmission patterns of dengue. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

3. Predicting reliability through structured expert elicitation with the repliCATS (Collaborative Assessments for Trustworthy Science) process

Author

Fraser, Hannah, primary, Bush, Martin, additional, Wintle, Bonnie C., additional, Mody, Fallon, additional, Smith, Eden T., additional, Hanea, Anca M., additional, Gould, Elliot, additional, Hemming, Victoria, additional, Hamilton, Daniel G., additional, Rumpff, Libby, additional, Wilkinson, David P., additional, Pearson, Ross, additional, Singleton Thorn, Felix, additional, Ashton, Raquel, additional, Willcox, Aaron, additional, Gray, Charles T., additional, Head, Andrew, additional, Ross, Melissa, additional, Groenewegen, Rebecca, additional, Marcoci, Alexandru, additional, Vercammen, Ans, additional, Parker, Timothy H., additional, Hoekstra, Rink, additional, Nakagawa, Shinichi, additional, Mandel, David R., additional, van Ravenzwaaij, Don, additional, McBride, Marissa, additional, Sinnott, Richard O., additional, Vesk, Peter, additional, Burgman, Mark, additional, and Fidler, Fiona, additional

Published
2023
Full Text
View/download PDF

6. Cytokine-induced cysteine- serine-rich nuclear protein-1 (CSRNP1) selectively contributes to MMP1 expression in human chondrocytes

Author

Macdonald, Christopher D., Falconer, Adrian M. D., Chan, Chun Ming, Wilkinson, David J., Skelton, Andrew, Reynard, Louise, Litherland, Gary J., Europe-Finner, G. Nicholas, and Rowan, Andrew D.

Subjects
Time Factors, Transcription, Genetic, Bioinformatics, lcsh:Medicine, Research and Analysis Methods, Biochemistry, Gene Expression Regulation, Enzymologic, Database and Informatics Methods, Chondrocytes, Animal Cells, Gene Types, Sequence Motif Analysis, Matrix Metalloproteinase 13, Medicine and Health Sciences, Genetics, Humans, Small interfering RNAs, Computer Simulation, lcsh:Science, Non-coding RNA, Promoter Regions, Genetic, Cells, Cultured, Connective Tissue Cells, Cell Nucleus, Activating Transcription Factor 3, Transcriptional Control, Messenger RNA, lcsh:R, Biology and Life Sciences, Cell Biology, Gene regulation, Nucleic acids, Biological Tissue, Cartilage, Connective Tissue, RNA, Regulator Genes, lcsh:Q, Gene expression, Anatomy, Cellular Types, Matrix Metalloproteinase 1, Apoptosis Regulatory Proteins, Sequence Analysis, Proto-Oncogene Proteins c-fos, Research Article, Interleukin-1
Abstract

Irreversible cartilage collagen breakdown by the collagenolytic matrix metalloproteinases (MMPs)-1 and MMP-13 represents a key event in pathologies associated with tissue destruction such as arthritis. Inflammation is closely associated with such pathology and occurs in both rheumatoid and osteoarthritis making it highly relevant to the prevailing tissue damage that characterises these diseases. The inflammation-induced activating protein-1 (AP-1) transcription factor is an important regulator of both MMP1 and MMP13 genes with interplay between signalling pathways contributing to their expression. Here, we have examined the regulation of MMP1 expression, and using in vivo chromatin immunoprecipitation analyses we have demonstrated that cFos bound to the AP-1 cis element within the proximal MMP1 promoter only when the gene was transcriptionally silent as previously observed for MMP13. Subsequent small interfering RNA-mediated silencing confirmed however, that cFos significantly contributes to MMP1 expression. In contrast, silencing of ATF3 (a prime MMP13 modulator) did not affect MMP1 expression whilst silencing of the Wnt-associated regulator cysteine- serine-rich nuclear protein-1 (CSRNP1) resulted in substantial repression of MMP1 but not MMP13. Furthermore, following an early transient peak in expression of CSRNP1 at the mRNA and protein levels similar to that seen for cFOS, CSRNP1 expression subsequently persisted unlike cFOS. Finally, DNA binding assays indicated that the binding of CSRNP1 to the AP-1 consensus-like sequences within the proximal promoter regions of MMP1 and MMP13 was preferentially selective for MMP1 whilst activating transcription factor 3 (ATF3) binding was exclusive to MMP13. These data further extend our understanding of the previously reported differential regulation of these MMP genes, and strongly indicate that although cFos modulates the expression of MMP1/13, downstream factors such as CSRNP1 and ATF3 ultimately serve as transcriptional regulators in the context of an inflammatory stimulus for these potent collagenolytic MMPs.

Published
2018

Catalog

Books, media, physical & digital resources
See catalog results