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6 results on '"Vu, Thu Trang"'

1. Correction: Prokaryotic soluble overexpression and purification of human VEGF165 by fusion to a maltose binding protein tag

Author

Nguyen, Minh Tan, primary, Krupa, Martin, additional, Koo, Bon-Kyung, additional, Song, Jung-A, additional, Vu, Thu Trang Thi, additional, Do, Bich Hang, additional, Nguyen, Anh Ngoc, additional, Seo, Taewook, additional, Yoo, Jiwon, additional, Jeong, Boram, additional, Jin, Jonghwa, additional, Lee, Kyung Jin, additional, Oh, Heung-Bum, additional, and Choe, Han, additional

Published
2017
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2. Prokaryotic Soluble Overexpression and Purification of Human VEGF165 by Fusion to a Maltose Binding Protein Tag

Author

Nguyen, Minh Tan, primary, Krupa, Martin, additional, Koo, Bon-Kyung, additional, Song, Jung-A, additional, Vu, Thu Trang Thi, additional, Do, Bich Hang, additional, Nguyen, Anh Ngoc, additional, Seo, Taewook, additional, Yoo, Jiwon, additional, Jeong, Boram, additional, Jin, Jonghwa, additional, Lee, Kyung Jin, additional, Oh, Heung-Bum, additional, and Choe, Han, additional

Published
2016
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4. Correction: Soluble Expression of Human Leukemia Inhibitory Factor with Protein Disulfide Isomerase in Escherichia coli and Its Simple Purification

Author

Song, Jung-A, primary, Koo, Bon-Kyung, additional, Chong, Seon-Ha, additional, Kim, Kyunhoo, additional, Choi, Dong Kyu, additional, Thi Vu, Thu Trang, additional, Nguyen, Minh Tan, additional, Jeong, Boram, additional, Ryu, Han-Bong, additional, Kim, Injune, additional, Jang, Yeon Jin, additional, Robinson, Robert Charles, additional, and Choe, Han, additional

Published
2014
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5. Soluble Expression of Human Leukemia Inhibitory Factor with Protein Disulfide Isomerase in Escherichia coli and Its Simple Purification

Author

Jung, A. Song, primary, Koo, Bon-Kyung, additional, Chong, Seon-Ha, additional, Kim, Kyunhoo, additional, Choi, Dong Kyu, additional, Thi Vu, Thu Trang, additional, Nguyen, Minh Tan, additional, Jeong, Boram, additional, Ryu, Han-Bong, additional, Kim, Injune, additional, Jang, Yeon Jin, additional, Robinson, Robert Charles, additional, and Choe, Han, additional

Published
2013
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6. Soluble Expression of Human Leukemia Inhibitory Factor with Protein Disulfide Isomerase in Escherichia coli and Its Simple Purification.

Author

Jung, A. Song, Koo, Bon-Kyung, Chong, Seon-Ha, Kim, Kyunhoo, Choi, Dong Kyu, Thi Vu, Thu Trang, Nguyen, Minh Tan, Jeong, Boram, Ryu, Han-Bong, Kim, Injune, Jang, Yeon Jin, Robinson, Robert Charles, and Choe, Han

Subjects
LEUKEMIA, GENE expression, PROTEIN disulfide isomerase, ESCHERICHIA coli, PLURIPOTENT stem cells, ENDOTOXINS
Abstract

Human leukemia inhibitory factor (hLIF) is a multifunctional cytokine that is essential for maintaining the pluripotency of embryonic stem cells. hLIF may be also be useful in aiding fertility through its effects on increasing the implantation rate of fertilized eggs. Thus these applications in biomedical research and clinical medicine create a high demand for bioactive hLIF. However, production of active hLIF is problematic since eukaryotic cells demonstrate limited expression and prokaryotic cells produce insoluble protein. Here, we have adopted a hybrid protein disulfide isomerase design to increase the solubility of hLIF in Escherichia coli. Low temperature expression of hLIF fused to the b'a' domain of protein disulfide isomerase (PDIb'a') increased the soluble expression in comparison to controls. A simple purification protocol for bioactive hLIF was established that includes removal of the PDIb'a' domain by cleavage by TEV protease. The resulting hLIF, which contains one extra glycine residue at the N-terminus, was highly pure and demonstrated endotoxin levels below 0.05 EU/μg. The presence of an intramolecular disulfide bond was identified using mass spectroscopy. This purified hLIF effectively maintained the pluripotency of a murine embryonic stem cell line. Thus we have developed an effective method to produce a pure bioactive version of hLIF in E. coli for use in biomedical research. [ABSTRACT FROM AUTHOR]

Published
2013
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