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1. Integrated analysis of DNA methylation profile of HLA-G gene and imaging in coronary heart disease: Pilot study.

Author

Concetta Schiano, Giuditta Benincasa, Teresa Infante, Monica Franzese, Rossana Castaldo, Carmela Fiorito, Gelsomina Mansueto, Vincenzo Grimaldi, Giovanni Della Valle, Gerardo Fatone, Andrea Soricelli, Giovanni Francesco Nicoletti, Antonio Ruocco, Ciro Mauro, Marco Salvatore, and Claudio Napoli

Subjects
Medicine, Science
Abstract

AIMS:Immune endothelial inflammation, underlying coronary heart disease (CHD) related phenotypes, could provide new insight into the pathobiology of the disease. We investigated DNA methylation level of the unique CpG island of HLA-G gene in CHD patients and evaluated the correlation with cardiac computed tomography angiography (CCTA) features. METHODS:Thirty-two patients that underwent CCTA for suspected CHD were enrolled for this study. Obstructive CHD group included fourteen patients, in which there was a stenosis greater than or equal to 50% in one or more of the major coronary arteries detected; whereas subjects with Calcium (Ca) Score = 0, uninjured coronaries and with no obstructive CHD (no critical stenosis, NCS) were considered as control subjects (n = 18). For both groups, DNA methylation profile of the whole 5'UTR-CpG island of HLA-G was measured. The plasma soluble HLA-G (sHLA-G) levels were detected in all subjects by specific ELISA assay. Statistical analysis was performed using R software. RESULTS:For the first time, our study reported that 1) a significant hypomethylation characterized three specific fragments (B, C and F) of the 5'UTR-CpG island (p = 0.05) of HLA-G gene in CHD patients compared to control group; 2) the hypomethylation level of one specific fragment of 161bp (+616/+777) positively correlated with coronary Ca score, a relevant parameter of CCTA (p

Published
2020
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2. Integrated analysis of DNA methylation profile of HLA-G gene and imaging in coronary heart disease: Pilot study

Author

Gelsomina Mansueto, Monica Franzese, Giovanni Francesco Nicoletti, Concetta Schiano, Vincenzo Grimaldi, Giuditta Benincasa, Carmela Fiorito, Antonio Ruocco, Giovanni Della Valle, Teresa Infante, Rossana Castaldo, Gerardo Fatone, Ciro Mauro, Claudio Napoli, Andrea Soricelli, Marco Salvatore, Schiano, C., Benincasa, G., Infante, T., Franzese, M., Castaldo, R., Fiorito, C., Mansueto, G., Grimaldi, V., Della Valle, G., Fatone, G., Soricelli, A., Nicoletti, G. F., Ruocco, A., Mauro, C., Salvatore, M., and Napoli, C.

Subjects
Male, 0301 basic medicine, Topography, Computed Tomography Angiography, Coronary Stenosi, Pilot Projects, Coronary Disease, Cardiovascular Medicine, 030204 cardiovascular system & hematology, Biochemistry, Vascular Medicine, Gastroenterology, Epigenesis, Genetic, Medical Conditions, HLA-G Antigen, 0302 clinical medicine, 5' Untranslated Region, Medicine and Health Sciences, Coronary Heart Disease, Coronary Vessel, Computed tomography angiography, Stenosis, Islands, Multidisciplinary, medicine.diagnostic_test, Chemical Reactions, Middle Aged, Lipids, Coronary Vessels, Chromatin, Plaque, Atherosclerotic, Nucleic acids, Chemistry, Cholesterol, medicine.anatomical_structure, CpG site, Cardiovascular Diseases, Physical Sciences, DNA methylation, Medicine, Epigenetics, Female, DNA modification, Case-Control Studie, Chromatin modification, Research Article, Chromosome biology, Human, Adult, Cell biology, medicine.medical_specialty, Science, Cardiology, Human leukocyte antigen, Methylation, 03 medical and health sciences, Signs and Symptoms, Internal medicine, Genetics, medicine, Humans, Pilot Project, Aged, HLA-G Antigens, Landforms, Treatment Guidelines, Health Care Policy, Biology and life sciences, business.industry, Coronary Stenosis, Case-control study, Geomorphology, DNA, DNA Methylation, medicine.disease, Health Care, Coronary arteries, 030104 developmental biology, Case-Control Studies, Earth Sciences, CpG Islands, Calcium, Gene expression, Clinical Medicine, 5' Untranslated Regions, CpG Island, business
Abstract

AIMS:Immune endothelial inflammation, underlying coronary heart disease (CHD) related phenotypes, could provide new insight into the pathobiology of the disease. We investigated DNA methylation level of the unique CpG island of HLA-G gene in CHD patients and evaluated the correlation with cardiac computed tomography angiography (CCTA) features. METHODS:Thirty-two patients that underwent CCTA for suspected CHD were enrolled for this study. Obstructive CHD group included fourteen patients, in which there was a stenosis greater than or equal to 50% in one or more of the major coronary arteries detected; whereas subjects with Calcium (Ca) Score = 0, uninjured coronaries and with no obstructive CHD (no critical stenosis, NCS) were considered as control subjects (n = 18). For both groups, DNA methylation profile of the whole 5'UTR-CpG island of HLA-G was measured. The plasma soluble HLA-G (sHLA-G) levels were detected in all subjects by specific ELISA assay. Statistical analysis was performed using R software. RESULTS:For the first time, our study reported that 1) a significant hypomethylation characterized three specific fragments (B, C and F) of the 5'UTR-CpG island (p = 0.05) of HLA-G gene in CHD patients compared to control group; 2) the hypomethylation level of one specific fragment of 161bp (+616/+777) positively correlated with coronary Ca score, a relevant parameter of CCTA (p

Published
2020
Full Text
View/download PDF

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