1. Tetracycline Susceptibility in Chlamydia suis Pig Isolates
- Author
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Fabien Vorimore, Manuela Donati, Antonietta Di Francesco, Nicole Borel, Federico Morandi, Karine Laroucau, Edoardo Vecchio Nepita, Andrea Balboni, Rachid Aaziz, Donati, M, Balboni, A, Laroucau, K, Aaziz, R, Vorimore, F, Borel, N, Morandi, F, Vecchio Nepita, E, Di Francesco, A, and University of Zurich
- Subjects
0301 basic medicine ,pig ,Swine ,lcsh:Medicine ,Gene Expression ,Artificial Gene Amplification and Extension ,Chlamydia sui ,Polymerase Chain Reaction ,Biochemistry ,law.invention ,Chlamydia Infection ,chemistry.chemical_compound ,law ,Antibiotics ,Nucleic Acids ,Gene expression ,Medicine and Health Sciences ,Chlamydia ,lcsh:Science ,Polymerase chain reaction ,Swine Diseases ,Mammals ,Multidisciplinary ,biology ,Antimicrobials ,Tetracycline Resistance ,Drugs ,Agriculture ,Anti-Bacterial Agents ,RNA, Ribosomal, 23S ,Real-time polymerase chain reaction ,Infectious Diseases ,Italy ,Tetracyclines ,Vertebrates ,medicine.drug ,Research Article ,DNA, Bacterial ,Livestock ,Tetracycline ,030106 microbiology ,Sexually Transmitted Diseases ,10184 Institute of Veterinary Pathology ,1100 General Agricultural and Biological Sciences ,Microbial Sensitivity Tests ,Real-Time Polymerase Chain Reaction ,Research and Analysis Methods ,Microbiology ,03 medical and health sciences ,Extraction techniques ,stomatognathic system ,Bacterial Proteins ,1300 General Biochemistry, Genetics and Molecular Biology ,Chlamydia suis ,Microbial Control ,medicine ,Genetics ,Animals ,TetR ,Gene Regulation ,Molecular Biology Techniques ,Molecular Biology ,Pharmacology ,1000 Multidisciplinary ,Bacteria ,lcsh:R ,Organisms ,Biology and Life Sciences ,DNA ,biochemical phenomena, metabolism, and nutrition ,Chlamydia Infections ,biology.organism_classification ,medicine.disease ,Virology ,RNA extraction ,Repressor Proteins ,chemistry ,reverse transcription ,570 Life sciences ,lcsh:Q ,real-time PCR - Abstract
The aims of the present study were to assess the prevalence of Chlamydia suis in an Italian pig herd, determine the tetracycline susceptibility of C. suis isolates, and evaluate tet(C) and tetR(C) gene expression. Conjunctival swabs from 20 pigs were tested for C. suis by real-time polymerase chain reaction, and 55% (11) were positive. C. suis was then isolated from 11 conjunctival swabs resampled from the same herd. All positive samples and isolates were positive for the tet(C) resistance gene. The in vitro susceptibility to tetracycline of the C. suis isolates showed MIC values ranging from 0.5 to 4 μg/mL. Tet(C) and tetR(C) transcripts were found in all the isolates, cultured both in the absence and presence of tetracycline. This contrasts with other Gram-negative bacteria in which both genes are repressed in the absence of the drug. Further investigation into tet gene regulation in C. suis is needed.
- Published
- 2015