1. B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections
- Author
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Rénier, Wendy, Bourdin, Arnaud, Rubbo, Pierre-Alain, Peries, Marianne, Dedieu, Luc, Bendriss, Sophie, Kremer, Laurent, Canaan, Stéphane, Terru, Dominique, Godreuil, Sylvain, Nagot, Nicolas, van de Perre, Philippe, Tuaillon, Edouard, Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU), Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Université de Montpellier (UM), Dynamique des interactions membranaires normales et pathologiques (DIMNP), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université Montpellier 1 (UM1), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Enzymologie interfaciale et de physiologie de la lipolyse (EIPL), Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Institut de Recherche en Infectiologie de Montpellier (IRIM), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie des systèmes macromoléculaires (LISM), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Département de Bactériologie-Virologie et Département d'Information Médicale, Service de Bactériologie-Virologie, Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Hôpital Arnaud de Villeneuve, Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), and MORNET, Dominique
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Male ,Bacterial Diseases ,B Cells ,Physiology ,health care facilities, manpower, and services ,[SDV]Life Sciences [q-bio] ,lcsh:Medicine ,Biochemistry ,White Blood Cells ,Animal Cells ,Immune Physiology ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,Medicine and Health Sciences ,Enzyme-Linked Immunoassays ,lcsh:Science ,Immune Response ,health care economics and organizations ,B-Lymphocytes ,Immune System Proteins ,Middle Aged ,Antibodies, Bacterial ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM] ,Enzymes ,[SDV] Life Sciences [q-bio] ,Actinobacteria ,Infectious Diseases ,BCG Vaccine ,Female ,Cellular Types ,Research Article ,Adult ,Immune Cells ,education ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Research and Analysis Methods ,Antibodies ,Bacterial Proteins ,Latent Tuberculosis ,Humans ,Tuberculosis ,Immunoassays ,Antibody-Producing Cells ,Aged ,Antigens, Bacterial ,Blood Cells ,Bacteria ,lcsh:R ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,Lipid Metabolism ,Tropical Diseases ,Memory B cells ,Immunoglobulin G ,Leukocytes, Mononuclear ,Immunologic Techniques ,Enzymology ,lcsh:Q ,Tomography, X-Ray Computed ,Carboxylic Ester Hydrolases ,Immunologic Memory ,Mycobacterium Tuberculosis - Abstract
International audience; BackgroundBetter understanding of the immune response directed against Mycobacterium tuberculosis (Mtb) is critical for development of vaccine strategies and diagnosis tests. Previous studies suggested that Mtb enzymes involved in lipid metabolism, are associated with persistence and/or reactivation of dormant bacilli.MethodsCirculating antibodies secreting cells (ASCs), memory B cells, and antibodies directed against Cut4 (Rv3452) and CFP21 (Rv1984c) antigens were explored in subjects with either active- or latent-tuberculosis (LTB), and in Mtb-uninfected individuals.ResultsCirculating anti-Cut4 ASCs were detected in 11/14 (78.6%) subjects from the active TB group vs. 4/17 (23.5%) from the LTB group (p = 0.001). Anti-CFP21 ASCs were found in 11/14 (78.6%) active TB vs. in 5/17 (29.4%) LTB cases (p = 0.01). Circulating anti-Cut4 and anti-CFP21 ASCs were not detected in 38 Mtb uninfected controls. Memory B cells directed against either Cut4 or CFP21 were identified in 8/11 (72.7%) and in 9/11 (81.8%) subjects with LTB infection, respectively, and in 2/6 Mtb uninfected individuals (33.3%). High level of anti-Cut4 and anti-CFP21 IgG were observed in active TB cases.ConclusionCirculating IgG SCs directed against Cut4 or CFP21 were mostly detected in patients presenting an active form of the disease, suggesting that TB reactivation triggers an immune response against these two antigens.
- Published
- 2017
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