503 results on '"Toro A"'
Search Results
2. Pathophysiology of non-cystic fibrosis bronchiectasis in children and adolescents with asthma: A protocol for systematic review and meta-analysis.
- Author
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Natali Caroline da Silva, Beatriz Cocato Malagutti, Joelia Maria Costa Dias Ladeira, Milena Baptistella Grotta, and Adyleia Aparecida Dalbo Contrera Toro
- Subjects
Medicine ,Science - Abstract
BackgroundThe pathophysiological mechanisms by which asthma and bronchiectasis are associated are still unclear. The association of these two diseases can result in more severe symptoms and a greater number of exacerbations.ObjectiveThe aim of this systematic review is to collect evidence of the pathophysiology of non-cystic fibrosis bronchiectasis with associated asthma in children and adolescents, aged 6-18 years old.MethodsA systematic and comprehensive search will be performed using eight main databases, PubMed, PubMed PMC, BVS/BIREME, Scopus, EMBASE, Cochrane Library, Scielo and Web of Science. Articles will be searched from the earliest available time to July 2023. The studied population will be composed of children and adolescents with asthma and non-cystic fibrosis bronchiectasis. From the data obtained, all articles found will be transferred to the Rayyan platform. Study selection will follow the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols Checklist (PRISMA P-2015). In addition, if sufficient data are available, a meta-analysis will be conducted. Two independent reviewers will conduct the studies selection, data extraction, and risk of bias assessment. The outcome measures will be to analyze if non-cystic fibrosis bronchiectasis is related to a specific inflammatory profile.DiscussionA systematic review will provide better knowledge about the etiopathogenesis and causes of the association between asthma and bronchiectasis and its role in the severity and control of asthma. Identifying, selecting and critically evaluating studies on asthma and bronchiectasis, would be possible to illuminate the characteristics of children and adolescents with associated diagnoses and provide information to help individualized treatments in order to control and prevent complications. The findings of this study will be published in a peer-reviewed journal.Systematic review registrationThe systematic review protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO) in July 2023 (registration number CRD42023440355).
- Published
- 2024
- Full Text
- View/download PDF
3. Hypertension subtypes at high altitude in Peru: Analysis of the Demographic and Family Health Survey 2016–2019
- Author
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Ortiz-Saavedra, Brando, primary, Montes-Madariaga, Elizbet S., additional, Moreno-Loaiza, Oscar, additional, and Toro-Huamanchumo, Carlos J., additional
- Published
- 2024
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4. Editing of SlWRKY29 by CRISPR-activation promotes somatic embryogenesis in Solanum lycopersicum cv. Micro-Tom
- Author
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Valencia-Lozano, Eliana, primary, Cabrera-Ponce, José Luis, additional, Barraza, Aarón, additional, López-Calleja, Alberto Cristian, additional, García-Vázquez, Elsa, additional, Rivera-Toro, Diana Marcela, additional, de Folter, Stefan, additional, and Alvarez-Venegas, Raúl, additional
- Published
- 2024
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5. Integrating niche and occupancy models to infer the distribution of an endemic fossorial snake (Atractus lasallei).
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Cruz-Arroyave, Camilo Alejandro, Toro-Cardona, Felipe A., and Parra, Juan Luis
- Subjects
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SPECIES distribution , *GRAZING , *AGRICULTURE , *SNAKES , *HABITATS - Abstract
Understanding species distribution and habitat preferences is crucial for effective conservation strategies. However, the lack of information about population responses to environmental change at different scales hinders effective conservation measures. In this study, we estimate the potential and realized distribution of Atractus lasallei, a semi-fossorial snake endemic to the northwestern region of Colombia. We modelled the potential distribution of A. lasallei based on ecological niche theory (using maxent), and habitat use was characterized while accounting for imperfect detection using a single-season occupancy model. Our results suggest that A. lasallei selects areas characterized by slopes below 10°, with high average annual precipitation (>2500mm/year) and herbaceous and shrubby vegetation. Its potential distribution encompasses the northern Central Cordillera and two smaller centers along the Western Cordillera, but its habitat is heavily fragmented within this potential distribution. When the two models are combined, the species' realized distribution sums up to 935 km2, highlighting its vulnerability. We recommend approaches that focus on variability at different spatio-temporal scales to better comprehend the variables that affect species' ranges and identify threats to vulnerable species. Prompt actions are needed to protect herbaceous and shrub vegetation in this region, highly demanded for agriculture and cattle grazing. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. Pathophysiology of non-cystic fibrosis bronchiectasis in children and adolescents with asthma: A protocol for systematic review and meta-analysis.
- Author
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da Silva, Natali Caroline, Malagutti, Beatriz Cocato, Ladeira, Joelia Maria Costa Dias, Grotta, Milena Baptistella, and Toro, Adyleia Aparecida Dalbo Contrera
- Subjects
ASTHMA in children ,BRONCHIECTASIS ,FIBROSIS ,PATHOLOGICAL physiology ,DATA extraction ,WHEEZE - Abstract
Background: The pathophysiological mechanisms by which asthma and bronchiectasis are associated are still unclear. The association of these two diseases can result in more severe symptoms and a greater number of exacerbations. Objective: The aim of this systematic review is to collect evidence of the pathophysiology of non-cystic fibrosis bronchiectasis with associated asthma in children and adolescents, aged 6–18 years old. Methods: A systematic and comprehensive search will be performed using eight main databases, PubMed, PubMed PMC, BVS/BIREME, Scopus, EMBASE, Cochrane Library, Scielo and Web of Science. Articles will be searched from the earliest available time to July 2023. The studied population will be composed of children and adolescents with asthma and non-cystic fibrosis bronchiectasis. From the data obtained, all articles found will be transferred to the Rayyan platform. Study selection will follow the Preferred Reporting Items for Systematic Reviews and Meta-Analysis Protocols Checklist (PRISMA P-2015). In addition, if sufficient data are available, a meta-analysis will be conducted. Two independent reviewers will conduct the studies selection, data extraction, and risk of bias assessment. The outcome measures will be to analyze if non-cystic fibrosis bronchiectasis is related to a specific inflammatory profile. Discussion: A systematic review will provide better knowledge about the etiopathogenesis and causes of the association between asthma and bronchiectasis and its role in the severity and control of asthma. Identifying, selecting and critically evaluating studies on asthma and bronchiectasis, would be possible to illuminate the characteristics of children and adolescents with associated diagnoses and provide information to help individualized treatments in order to control and prevent complications. The findings of this study will be published in a peer-reviewed journal. Systematic review registration: The systematic review protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO) in July 2023 (registration number CRD42023440355). [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
7. Proximal and fatty acid analysis in Ostrea chilensis, Crassostrea gigas and Mytilus chilensis (Bivalvia: Mollusca) from southern Chile.
- Author
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Andrea Valenzuela, Pablo A Oyarzún, Jorge E Toro, Jorge M Navarro, Oscar Ramírez, and Ana Farias
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Medicine ,Science - Abstract
Oysters and blue mussels are important hydrobiological resources for aquaculture. In Chile, they are farming on the Chiloé island, where around 18% of the world's mussels are produced, however, their nutritional dynamics are largely unknown. For this reason, the objective of this study was to determine the proximal biochemical composition and the fatty acid profile in the Chilean oyster (Ostrea chilensis), the Pacific oyster (Crassostrea gigas) and the Chilean mussel (Mytilus chilensis), to perform an intra and interspecific comparison. Shellfish sampled in winter were characterized by a high protein content, followed by medium values for lipid content and a low carbohydrate content compared to similar species in Europe. Also, oysters and mussels were found to be rich in omega-3 long chain polyunsaturated fatty acid (n-3 LC-PUFA), so they can be considered excellent functional food option for a healthy human diet. Their high contribution of n-3 LC-PUFA ranged between 5.2-12.9 μg FA mg-1 dry weight with high n-3/n-6 ratios, which depends on both the species and the on-growing location. Both taxa can be considered a plausible option to promote a healthy diet of marine origin in future generations. Also, these results could benefit the projection and development of aquaculture of these mollusks.
- Published
- 2022
- Full Text
- View/download PDF
8. Latent class analyses of multimorbidity and all-cause mortality: A prospective study in Chilean adults
- Author
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Nazar, Gabriela, primary, Díaz-Toro, Felipe, additional, Concha-Cisternas, Yeny, additional, Leiva-Ordoñez, Ana María, additional, Troncoso-Pantoja, Claudia, additional, Celis-Morales, Carlos, additional, and Petermann-Rocha, Fanny, additional
- Published
- 2023
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9. Endogenous expression of inactive lysine deacetylases reveals deacetylation-dependent cellular mechanisms
- Author
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Toro, Tasha B., primary, Skripnikova, Elena V., additional, Bornes, Kiara E., additional, Zhang, Kun, additional, and Watt, Terry J., additional
- Published
- 2023
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10. Characterization of the external exposome and its contribution to the clinical respiratory and early biological effects in children: The PROMESA cohort study protocol
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Marín, Diana, primary, Orozco, Luz Yaneth, additional, Narváez, Diana María, additional, Ortiz-Trujillo, Isabel Cristina, additional, Molina, Francisco José, additional, Ramos, Carlos Daniel, additional, Rodriguez-Villamizar, Laura, additional, Bangdiwala, Shrikant I., additional, Morales, Olga, additional, Cuellar, Martha, additional, Hernández, Luis Jorge, additional, Henao, Enrique Antonio, additional, Lopera, Verónica, additional, Corredor, Andrea, additional, Toro, María Victoria, additional, Groot, Helena, additional, Villamil-Osorio, Milena, additional, Muñoz, Diego Alejandro, additional, Hincapié, Roberto Carlos, additional, Amaya, Ferney, additional, Oviedo, Ana Isabel, additional, López, Lucelly, additional, Morales-Betancourt, Ricardo, additional, Marín-Ochoa, Beatriz Elena, additional, Sánchez-García, Oscar Eduardo, additional, Marín, Juan Sebastián, additional, Abad, José Miguel, additional, Toro, Julio Cesar, additional, Pinzón, Eliana, additional, Builes, Juan José, additional, and Rueda, Zulma Vanessa, additional
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- 2023
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11. Characterization of morphological and biological aspects of venomous caterpillars of the genus Lonomia Walker (Lepidoptera: Saturniidae) in Colombia
- Author
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Toro-Vargas, Diana M., primary, González, Camila, additional, Rougerie, Rodolphe, additional, and Amarillo-Suárez, Angela R., additional
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- 2023
- Full Text
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12. B.Y.O. Bees: Managing wild bee biodiversity in urban greenspaces
- Author
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Anderson, Maggie, primary, Crubaugh, Floréal, additional, Greenslit, Cady, additional, Hill, Emily, additional, Kroth, Heidi, additional, Stanislawski, Emily, additional, Ribbons, Relena, additional, and Del Toro, Israel, additional
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- 2023
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13. Characterization of the external exposome and its contribution to the clinical respiratory and early biological effects in children: The PROMESA cohort study protocol
- Author
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Diana Marín, Luz Yaneth Orozco, Diana María Narváez, Isabel Cristina Ortiz-Trujillo, Francisco José Molina, Carlos Daniel Ramos, Laura Rodriguez-Villamizar, Shrikant I. Bangdiwala, Olga Morales, Martha Cuellar, Luis Jorge Hernández, Enrique Antonio Henao, Verónica Lopera, Andrea Corredor, María Victoria Toro, Helena Groot, Milena Villamil-Osorio, Diego Alejandro Muñoz, Roberto Carlos Hincapié, Ferney Amaya, Ana Isabel Oviedo, Lucelly López, Ricardo Morales-Betancourt, Beatriz Elena Marín-Ochoa, Oscar Eduardo Sánchez-García, Juan Sebastián Marín, José Miguel Abad, Julio Cesar Toro, Eliana Pinzón, Juan José Builes, and Zulma Vanessa Rueda
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Multidisciplinary - Abstract
Background Air pollution contains a mixture of different pollutants from multiple sources. However, the interaction of these pollutants with other environmental exposures, as well as their harmful effects on children under five in tropical countries, is not well known. Objective This study aims to characterize the external exposome (ambient and indoor exposures) and its contribution to clinical respiratory and early biological effects in children. Materials and methods A cohort study will be conducted on children under five (n = 500) with a one-year follow-up. Enrolled children will be followed monthly (phone call) and at months 6 and 12 (in person) post-enrolment with upper and lower Acute Respiratory Infections (ARI) examinations, asthma development, asthma control, and genotoxic damage. The asthma diagnosis will be pediatric pulmonologist-based and a standardized protocol will be used. Exposure, effect, and susceptibility biomarkers will be measured on buccal cells samples. For environmental exposures PM2.5 will be sampled, and questionnaires, geographic information, dispersion models and Land Use Regression models for PM2.5 and NO2 will be used. Different statistical methods that include Bayesian and machine learning techniques will be used for the ambient and indoor exposures-and outcomes. This study was approved by the ethics committee at Universidad Pontificia Bolivariana. Expected study outcomes/findings To estimate i) The toxic effect of particulate matter transcending the approach based on pollutant concentration levels; ii) The risk of developing an upper and lower ARI, based on different exposure windows; iii) A baseline of early biological damage in children under five, and describe its progression after a one-year follow-up; and iv) How physical and chemical PM2.5 characteristics influence toxicity and children’s health.
- Published
- 2023
14. Adaptation and psychometric properties of the school engagement and contextual factors questionnaires for Covid-19 and post Covid-19 context
- Author
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Lara, Laura, primary, Saracostti, Mahia, additional, and de-Toro, Ximena, additional
- Published
- 2022
- Full Text
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15. Oropouche virus infection in patients with acute febrile syndrome: Is a predictive model based solely on signs and symptoms useful?
- Author
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Durango-Chavez, Hilda V., primary, Toro-Huamanchumo, Carlos J., additional, Silva-Caso, Wilmer, additional, Martins-Luna, Johanna, additional, Aguilar-Luis, Miguel Angel, additional, del Valle-Mendoza, Juana, additional, and Puyen, Zully M., additional
- Published
- 2022
- Full Text
- View/download PDF
16. Proximal and fatty acid analysis in Ostrea chilensis, Crassostrea gigas and Mytilus chilensis (Bivalvia: Mollusca) from southern Chile
- Author
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Valenzuela, Andrea, primary, Oyarzún, Pablo A., additional, Toro, Jorge E., additional, Navarro, Jorge M., additional, Ramírez, Oscar, additional, and Farias, Ana, additional
- Published
- 2022
- Full Text
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17. B.Y.O. Bees: Managing wild bee biodiversity in urban greenspaces
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Maggie Anderson, Floréal Crubaugh, Cady Greenslit, Emily Hill, Heidi Kroth, Emily Stanislawski, Relena Ribbons, and Israel Del Toro
- Subjects
Multidisciplinary - Abstract
As cities become more populated and the density of urban development increases, local biodiversity is threatened. Urban greenspaces have the capacity to preserve pollinator biodiversity, but the quality of support they provide depends on greenspace landscape attributes, including the availability of pollinator habitat and foraging resources. Wild native bees provide important pollination services to urban ecosystems, yet relatively little is known about how urban landscape management influences pollinator community composition and diversity. Our study explores how wild bee communities are affected by greenspace and landscape-level features like pollinator management practices, in urban greenspaces in and around Appleton Wisconsin: a mid-sized urban community spanning more than 100 sq. km. We sampled and identified native bees periodically between late-May 2017 and mid-September of 2018 using standardized arrays of pan traps at 15 sites around the city. We classified greenspaces based on their level of development (urban or suburban) and whether they were managed or unmanaged for increasing wild pollinator diversity. We quantified floral species diversity, floral color diversity, tree species diversity, and proximity of sites to open water for each site and used remotely sensed satellite data from both the USGS National Land Cover Database (NLCD) and the Normalized Difference Vegetation Index (NDVI). All variables were tested as potential correlates of wild bee abundance and species richness. Active pollinator management sites supported higher levels of bee abundance and richness. Notably, active greenspace management (e.g. planting native wildflowers) was a stronger correlate of bee abundance and richness than greenspace size and other landscape-level attributes. Within-greenspace attributes such as floral diversity, tree diversity, and proximity to open water contributed positively to both bee abundance and richness. Based on these findings, we suggest that urban greenspaces may be managed more efficiently and cost-effectively by focusing resources on active management by planting wildflowers, removing invasive species, creating nesting habitat, and providing water resources, rather than simply expanding in area.
- Published
- 2023
18. Efficacy and safety of different antimicrobial DURATions for the treatment of Infections associated with Osteosynthesis Material implanted after long bone fractures (DURATIOM): Protocol for a randomized, pragmatic trial.
- Author
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Garrigós, Carmen, Rosso-Fernández, Clara María, Borreguero, Irene, Rodríguez, Patricia, García-Albea, Raquel, Bravo-Ferrer, Jose María, Rodríguez-Baño, Jesús, and del Toro, María Dolores
- Subjects
BONE fractures ,TREATMENT duration ,INTERNAL fixation in fractures ,FRACTURE healing ,CLINICAL trials ,DEEP brain stimulation - Abstract
Background: Infection associated with osteosynthesis material (IOM) is one of the most feared and challenging complications of trauma surgery and can cause significant functional loss, requiring multiple interventions and excessive consumption of antimicrobials. Evidence is needed about the best surgical procedure and the duration of antibiotic treatment according to the age of the implant or onset of infection symptoms, as it considers the biofilm formation and the state of fracture healing. There were not clinical trials evaluating the optimal duration of antibiotic therapy in IOM when implant is retained. Because there are antibiotics that have proven to be effective for the treatment of infection associated to implant, mainly in PJI, these antibiotics could be used in these infections. Investigating whether shorter duration of treatment is a priority in infectious diseases, as a way to reduce the exposure to antibiotics and help in controlling antimicrobial resistance and avoiding unnecessary adverse events and cost. We aim to describe the hypothesis, objectives, design, variables and procedures for a pragmatic randomized controlled trial comparing different durations of antibiotic treatment in IOM after long bone fractures treated with debridement and implant retention. Methods and design: This is a multicenter, open-label, non-inferiority, randomized, controlled, pragmatic phase 3 trial, comparing different durations of antibiotic treatment in IOM after long bone fractures treated with debridement and implant retention. Patients with microbiologically confirmed IOM will be included. Eligible patients are those older than 14 years, with early IOM (up to 2 weeks after the implant surgery) and delayed IOM (between 3 and 10 weeks after the implant surgery) with stabilized fracture and absence of bone exposure who sign the informed consent. Randomization will be 1:1 to receive a short-term antibiotic treatment (8 weeks in early IOM and 12 weeks in delayed IOM) or a long-term antibiotic treatment (12 weeks in early IOM or until fracture healing or implant removal in delayed IOM). The antibiotic treatment will be that used in routine practice by the specialist in infectious diseases. The primary outcome is the composited variable "cure" that includes clinical cure, radiological healing, and definitive soft tissue coverage, which will be evaluated in the test of cure at 12 months after the end of antibiotic therapy. Adverse events, resistance development during therapy and functional status will be collected. A total of 364 patients are needed to show a 10% non-inferiority margin, with 80% power and 5% one-sided significance level. Discussion: If the hypothesis of non-inferiority of short vs. long antibiotic treatments is demonstrated, and the efficacy of antibiotics with less ecological impact in long treatments, the impact on reduction of bacterial resistance, toxicity and health costs will be observed. Trial registration: This trial is registered at ClinicalTrials.gov (NCT05294796) on Jan 26
th 2022 and at the European Union Drug Regulating Authorities Clinical Trials (EUDRACT) (2021-003914-38) on Jul 16th 2021. The Sponsor Study Code is DURATIOM. [ABSTRACT FROM AUTHOR]- Published
- 2023
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19. A specific structure and high richness characterize intestinal microbiota of HIV-exposed seronegative individuals
- Author
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Juan F. Alzate, Wildeman Zapata, María Teresa Rugeles, Natalia A. Taborda, Juan C. Hernandez, Eduardo E. Zurek, Jorge A Luján, Tulio J. Lopera, Wbeimar Aguilar-Jimenez, and Miguel A. Toro
- Subjects
RNA viruses ,Male ,Prevotella ,HIV Infections ,Pathology and Laboratory Medicine ,T-Lymphocytes, Regulatory ,Feces ,Immunodeficiency Viruses ,Cellular types ,Multidisciplinary ,biology ,Immune cells ,virus diseases ,Regulatory T cells ,Genomics ,Butyrivibrio ,Middle Aged ,Medical Microbiology ,Viral Pathogens ,Viruses ,Medicine ,White blood cells ,Female ,Anatomy ,Pathogens ,Research Article ,Adult ,Cell biology ,Blood cells ,Adolescent ,Science ,Megasphaera ,Immunology ,T cells ,Alpha (ethology) ,Microbial Genomics ,Research and Analysis Methods ,Microbiology ,Young Adult ,Immune system ,HIV Seronegativity ,Retroviruses ,medicine ,Genetics ,Humans ,Seroconversion ,Molecular Biology Techniques ,Microbial Pathogens ,Molecular Biology ,Medicine and health sciences ,Bacteria ,Gut Bacteria ,Lentivirus ,Organisms ,Biology and Life Sciences ,HIV ,biology.organism_classification ,medicine.disease ,Gastrointestinal Microbiome ,Gastrointestinal Tract ,Animal cells ,Case-Control Studies ,HIV-1 ,Th17 Cells ,Microbiome ,Bacteroides ,Dysbiosis ,Digestive System ,Cloning - Abstract
Intestinal microbiota facilitates food breakdown for energy metabolism and influences the immune response, maintaining mucosal homeostasis. Overall, HIV infection is associated with intestinal dysbiosis and immune activation, which has been related to seroconversion in HIV-exposed individuals. However, it is unclear whether microbiota dysbiosis is the cause or the effect of immune alterations and disease progression or if it could modulate the risk of acquiring the HIV infection. We characterize the intestinal microbiota and determine its association with immune regulation in HIV-exposed seronegative individuals (HESN), HIV-infected progressors (HIV+), and healthy control (HC) subjects. For this, feces and blood were collected. The microbiota composition of HESN showed a significantly higher alpha (p = 0.040) and beta diversity (p = 0.006) compared to HC, but no differences were found compared to HIV+. A lower Treg percentage was observed in HESN (1.77%) than HC (2.98%) and HIV+ (4.02%), with enrichment of the genus Butyrivibrio (p = 0.029) being characteristic of this profile. Moreover, we found that Megasphaera (p = 0.017) and Victivallis (p = 0.0029) also are enriched in the microbiota composition in HESN compared to HC and HIV+ subjects. Interestingly, an increase in Succinivibrio and Prevotella, and a reduction in Bacteroides genus, which is typical of HIV-infected individuals, were observed in both HESN and HIV+, compared to HC. Thus, HESNs have a microbiota profile, similar to that observed in HIV+, most likely because HESN are cohabiting with their HIV+ partners.
- Published
- 2021
20. A specific structure and high richness characterize intestinal microbiota of HIV-exposed seronegative individuals
- Author
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Lopera, Tulio J., primary, Lujan, Jorge A., additional, Zurek, Eduardo, additional, Zapata, Wildeman, additional, Hernandez, Juan C., additional, Toro, Miguel A., additional, Alzate, Juan F., additional, Taborda, Natalia A., additional, Rugeles, Maria T., additional, and Aguilar-Jimenez, Wbeimar, additional
- Published
- 2021
- Full Text
- View/download PDF
21. Neural correlates of acoustic dissonance in music: The role of musicianship, schematic and veridical expectations
- Author
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Pagès-Portabella, Carlota, primary, Bertolo, Mila, additional, and Toro, Juan M., additional
- Published
- 2021
- Full Text
- View/download PDF
22. Proximal and fatty acid analysis in Ostrea chilensis, Crassostrea gigas and Mytilus chilensis (Bivalvia: Mollusca) from southern Chile
- Author
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Andrea Valenzuela, Pablo A. Oyarzún, Jorge E. Toro, Jorge M. Navarro, Oscar Ramírez, and Ana Farias
- Subjects
Mytilus ,Multidisciplinary ,Fatty Acids ,Ostrea ,Animals ,Humans ,Chile ,Crassostrea ,Bivalvia - Abstract
Oysters and blue mussels are important hydrobiological resources for aquaculture. In Chile, they are farming on the Chiloé island, where around 18% of the world’s mussels are produced, however, their nutritional dynamics are largely unknown. For this reason, the objective of this study was to determine the proximal biochemical composition and the fatty acid profile in the Chilean oyster (Ostrea chilensis), the Pacific oyster (Crassostrea gigas) and the Chilean mussel (Mytilus chilensis), to perform an intra and interspecific comparison. Shellfish sampled in winter were characterized by a high protein content, followed by medium values for lipid content and a low carbohydrate content compared to similar species in Europe. Also, oysters and mussels were found to be rich in omega-3 long chain polyunsaturated fatty acid (n-3 LC-PUFA), so they can be considered excellent functional food option for a healthy human diet. Their high contribution of n-3 LC-PUFA ranged between 5.2–12.9 μg FA mg-1 dry weight with high n-3/n-6 ratios, which depends on both the species and the on-growing location. Both taxa can be considered a plausible option to promote a healthy diet of marine origin in future generations. Also, these results could benefit the projection and development of aquaculture of these mollusks.
- Published
- 2021
23. Neural correlates of acoustic dissonance in music: The role of musicianship, schematic and veridical expectations
- Author
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Juan M. Toro, Carlota Pagès-Portabella, and Mila Bertolo
- Subjects
Physiology ,Emotions ,Social Sciences ,Event-Related Potentials ,Cognitive dissonance ,Medicine and Health Sciences ,Psychology ,Attention ,Child ,Clinical Neurophysiology ,Grammar ,Brain Mapping ,Multidisciplinary ,Physics ,Brain ,Cognition ,Electroencephalography ,Consonance and dissonance ,Music Perception ,Electrophysiology ,Bioassays and Physiological Analysis ,Brain Electrophysiology ,Physical Sciences ,Auditory Perception ,Evoked Potentials, Auditory ,Medicine ,Female ,Sensory Perception ,Cognitive psychology ,Research Article ,Adult ,Adolescent ,Imaging Techniques ,Science ,Neurophysiology ,Neuroimaging ,Research and Analysis Methods ,P3a ,P3b ,Humans ,Syntax ,Neural correlates of consciousness ,Music Cognition ,Electrophysiological Techniques ,Cognitive Psychology ,Biology and Life Sciences ,Linguistics ,Acoustics ,Acoustic Stimulation ,Chord (music) ,Cognitive Science ,Perception ,Clinical Medicine ,Bioacoustics ,Deviance (sociology) ,Music ,Neuroscience - Abstract
In western music, harmonic expectations can be fulfilled or broken by unexpected chords. Musical irregularities in the absence of auditory deviance elicit well-studied neural responses (e.g. ERAN, P3, N5). These responses are sensitive to schematic expectations (induced by syntactic rules of chord succession) and veridical expectations about predictability (induced by experimental regularities). However, the cognitive and sensory contributions to these responses and their plasticity as a result of musical training remains under debate. In the present study, we explored whether the neural processing of pure acoustic violations is affected by schematic and veridical expectations. Moreover, we investigated whether these two factors interact with long-term musical training. In Experiment 1, we registered the ERPs elicited by dissonant clusters placed either at the middle or the ending position of chord cadences. In Experiment 2, we presented to the listeners with a high proportion of cadences ending in a dissonant chord. In both experiments, we compared the ERPs of musicians and non-musicians. Dissonant clusters elicited distinctive neural responses (an early negativity, the P3 and the N5). While the EN was not affected by syntactic rules, the P3a and P3b were larger for dissonant closures than for middle dissonant chords. Interestingly, these components were larger in musicians than in non-musicians, while the N5 was the opposite. Finally, the predictability of dissonant closures in our experiment did not modulate any of the ERPs. Our study suggests that, at early time windows, dissonance is processed based on acoustic deviance independently of syntactic rules. However, at longer latencies, listeners may be able to engage integration mechanisms and further processes of attentional and structural analysis dependent on musical hierarchies, which are enhanced in musicians.
- Published
- 2021
24. Adaptation and psychometric properties of the school engagement and contextual factors questionnaires for Covid-19 and post Covid-19 context
- Author
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Laura Lara, Mahia Saracostti, Ximena de-Toro, and Universidad de Sevilla. Departamento de Psicología Evolutiva y de la Educación
- Subjects
Male ,Schools ,Multidisciplinary ,Psychometrics ,Surveys and Questionnaires ,COVID-19 ,Humans ,Female ,Child ,Students - Abstract
School engagement has been demonstrated to be a relevant aspect in promoting students’ successful trajectories, a commitment that in its turn is influenced by contextual factors (family, teachers, and peers). Having instruments to measure these constructs allows decisions to be made to improve student retention, especially relevant in the context of uncertainty caused by covid-19. The aim of the study was to adapt and analyze the psychometric properties of questionnaires used to measure school engagement and contextual factors in the context of the pandemic with elementary school students in Chile. After adaptation of the instruments, through expert evaluation and focus groups with students, they were administered to 579 students in seventh and eighth grade (mean age = 12.79, 52% were boys), and to 334 students in fifth and sixth grade (mean age = 11.35, 38% were boys) in Chile. Confirmatory factor analyses showed that the two versions of the school engagement measurement instrument had an adequate fit with the original model of three correlated factors, cognitive, affective, and behavioral commitment. Similarly, these two versions of the instrument measuring the contextual factors had a good fit with the original model of three correlated factors, family, teachers, and peers. In addition, both versions of both questionnaires presented appropriate levels of internal consistency.
- Published
- 2022
25. Oropouche virus infection in patients with acute febrile syndrome: Is a predictive model based solely on signs and symptoms useful?
- Author
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Hilda V. Durango-Chavez, Carlos J. Toro-Huamanchumo, Wilmer Silva-Caso, Johanna Martins-Luna, Miguel Angel Aguilar-Luis, Juana del Valle-Mendoza, and Zully M. Puyen
- Subjects
Models, Statistical ,Orthobunyavirus ,Multidisciplinary ,Fever ,Humans ,Bunyaviridae Infections ,Prognosis - Abstract
Background Oropouche fever is an infectious disease caused by the Oropouche virus (OROV). The diagnosis and prediction of the clinical picture continue to be a great challenge for clinicians who manage patients with acute febrile syndrome. Several symptoms have been associated with OROV virus infection in patients with febrile syndrome; however, to date, there is no clinical prediction rule, which is a fundamental tool to help the approach of this infectious disease. Objective To assess the performance of a prediction model based solely on signs and symptoms to diagnose Oropouche virus infection in patients with acute febrile syndrome. Materials and methods Validation study, which included 923 patients with acute febrile syndrome registered in the Epidemiological Surveillance database of three arbovirus endemic areas in Peru. Results A total of 97 patients (19%) were positive for OROV infection in the development group and 23.6% in the validation group. The area under the curve was 0.65 and the sensitivity, specificity, PPV, NPV, LR + and LR- were 78.2%, 35.1%, 27.6%, 83.6%, 1.20 and 0.62, respectively. Conclusions The development of a clinical prediction model for the diagnosis of Oropouche based solely on signs and symptoms does not work well. This may be due to the fact that the symptoms are nonspecific and related to other arbovirus infections, which confuse and make it difficult to predict the diagnosis, especially in endemic areas of co-infection of these diseases. For this reason, epidemiological surveillance of OROV in various settings using laboratory tests such as PCR is important.
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- 2022
26. Inflammasome genes polymorphisms may influence the development of hepatitis C in the Amazonas, Brazil
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Nadja Pinto Garcia, Juliana dos Santos Affonso, Aya Sadahiro, Jéssica Albuquerque da Silva, Adriana Malheiro, Hanna Lara Silva Negreiros Dray, Rajendranath Ramasawmy, Mauricio Morishi Ogusku, Flamir da Silva Victoria, Diana Mota Toro, Grenda Leite Pereira, Pedro Vieira da Silva Neto, Allyson Guimarães Costa, Keyla Santos de Sousa, Marilú Victória Barbieri, Eduardo Antônio Donadi, Andréa Monteiro Tarragô, and Priscila Santos Sarmento
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RNA viruses ,Male ,Linkage disequilibrium ,Heredity ,Gastroenterology and hepatology ,Inflammasomes ,Physiology ,Interleukin-1beta ,Hepacivirus ,medicine.disease_cause ,Biochemistry ,Homozygosity ,Hepatitis ,Cathepsin B ,Gene Frequency ,Fibrosis ,Polymorphism (computer science) ,Immune Physiology ,Genotype ,Medicine ,Pathology and laboratory medicine ,Innate Immune System ,Multidisciplinary ,Immune System Proteins ,Heterozygosity ,Hepatitis C virus ,Interleukin-18 ,Hepatitis C ,Medical microbiology ,Middle Aged ,Neoplasm Proteins ,DNA-Binding Proteins ,Infectious hepatitis ,Viruses ,Infectious diseases ,Cytokines ,Interleukin 18 ,Female ,Pathogens ,Brazil ,Research Article ,Medical conditions ,Adult ,Science ,Immunology ,Viral diseases ,Microbiology ,Polymorphism, Single Nucleotide ,Young Adult ,NLR Family, Pyrin Domain-Containing 3 Protein ,Genetics ,Humans ,Genetic Predisposition to Disease ,Allele ,Alleles ,Liver diseases ,Aged ,Medicine and health sciences ,Flaviviruses ,business.industry ,GENÓTIPOS ,Organisms ,Viral pathogens ,Biology and Life Sciences ,Proteins ,Molecular Development ,medicine.disease ,Hepatitis viruses ,Microbial pathogens ,CARD Signaling Adaptor Proteins ,Genetic Loci ,Immune System ,business ,Developmental Biology - Abstract
Hepatitis C is considered a major public health problem caused by the hepatitis C virus (HCV). Viral infections are known to induce production of IL1β through the signaling pathway of inflammasomes. Emerging evidences suggest that Inflammasome genes may influence the immune response against HCV as the host genetic background may contribute to the balance between acute and chronic inflammation. We investigated in 151 patients with chronic hepatitis C and 206 healthy blood donors’ individuals (HD). Polymorphisms in the IL1B and IL18 genes were genotyped by PCR-RFLP, while NLRP3, CARD8, CTSB and AIM2 by RT- PCR. Serum assay of IL-1β cytokine was performed by ELISA. 84 patients presented mild fibrosis (NLRP3-rs10754558 C/C genotype correlated with higher IL-1β levels compared to the G/G genotype. Similar pattern was observed in patients with hepatitis C, mean circulating IL-1β levels were 21,96 ± 4.5 and 10,62 ± 3.3pg/mL among the C/C and G/G genotypes, respectively. This pattern holds even after stratification of the patients into mild fibrosis and advanced fibrosis, demonstrating that the NLRP3-rs10754558 or another polymorphism in linkage disequilibrium with it possibly has an influence on the processing of pro-IL-1β. Notably, higher levels of IL-1β (Mann–Whitney test, pCARD8-rs2009373 and IL1B-rs16944 are less prone to hepatitis C development (padj = 0.039). Similarly, herozygote carriers for CTSB-rs1692816 and AIM2-rs1103577 (padj = 0.008) or for IL18-rs187238 and NLRP3-rs10754558 (padj = 0.005), have less chances to the development of hepatitis C. However, between subgroups of CARD8-rs2009373 and heterozygous for IL18-rs187238 (padj = 0.028), have mild form of fibrosis.
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- 2021
27. Blue mussels of the Mytilus edulis species complex from South America: The application of species delimitation models to DNA sequence variation
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Oyarzún, Pablo A., primary, Toro, Jorge E., additional, Nuñez, José J., additional, Suárez-Villota, Elkin Y., additional, and Gardner, Jonathan P. A., additional
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- 2021
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28. SUMO conjugation susceptibility of Akt/protein kinase B affects the expression of the pluripotency transcription factor Nanog in embryonic stem cells
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Francia, Marcos, primary, Stortz, Martin, additional, Echegaray, Camila Vazquez, additional, Oses, Camila, additional, Verneri, Paula, additional, Petrone, María Victoria, additional, Toro, Ayelen, additional, Waisman, Ariel, additional, Miriuka, Santiago, additional, Cosentino, María Soledad, additional, Levi, Valeria, additional, and Guberman, Alejandra, additional
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- 2021
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29. Blue mussels of the Mytilus edulis species complex from South America: The application of species delimitation models to DNA sequence variation
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Elkin Y. Suárez-Villota, Jorge E. Toro, Jonathan P. A. Gardner, José J. Nuñez, and Pablo A. Oyarzún
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Topography ,Species Delimitation ,Mytilus edulis ,Speciation ,Geographical locations ,Mussels ,Chile ,Phylogeny ,Data Management ,Islands ,Multidisciplinary ,Eukaryota ,Phylogenetic Analysis ,Mytilus ,Phylogenetics ,Falkland Islands ,Genes, Mitochondrial ,Genetic structure ,Medicine ,Taxonomy (biology) ,Female ,Research Article ,Species complex ,Bivalves ,Computer and Information Sciences ,Nuclear gene ,animal structures ,Evolutionary Processes ,Science ,Argentina ,Zoology ,Locus (genetics) ,Biology ,DNA, Ribosomal ,Electron Transport Complex IV ,Species Specificity ,Animals ,Evolutionary Systematics ,Taxonomy ,Evolutionary Biology ,Landforms ,Chile (Country) ,Base Sequence ,Cytochrome c oxidase subunit I ,fungi ,Organisms ,Genetic Variation ,Biology and Life Sciences ,Bayes Theorem ,Geomorphology ,Molluscs ,South America ,biology.organism_classification ,Invertebrates ,Haplotypes ,Genetic Loci ,Earth Sciences ,Uruguay ,People and places - Abstract
Smooth-shelled blue mussels, Mytilus spp., have a worldwide antitropical distribution and are ecologically and economically important. Mussels of the Mytilus edulis species complex have been the focus of numerous taxonomic and biogeographical studies, in particular in the Northern hemisphere, but the taxonomic classification of mussels from South America remains unclear. The present study analysed 348 mussels from 20 sites in Argentina, Chile, Uruguay and the Falkland Islands on the Atlantic and Pacific coasts of South America. We sequenced two mitochondrial locus, Cytochrome c Oxidase subunit I (625 bp) and 16S rDNA (443 bp), and one nuclear gene, ribosomal 18S rDNA (1770 bp). Mitochondrial and nuclear loci were analysed separately and in combination using maximum likelihood and Bayesian inference methods to identify the combination of the most informative dataset and model. Species delimitation using five different models (GMYC single, bGMYC, PTP, bPTP and BPP) revealed that the Mytilus edulis complex in South America is represented by three species: native M. chilensis, M. edulis, and introduced Northern Hemisphere M. galloprovincialis. However, all models failed to delimit the putative species Mytilus platensis. In contrast, however, broad spatial scale genetic structure in South America using Geneland software to analyse COI sequence variation revealed a group of native mussels (putatively M. platensis) in central Argentina and the Falkland Islands. We discuss the scope of species delimitation methods and the use of nuclear and mitochondrial genetic data to the recognition of species within the Mytilus edulis complex at regional and global scales.
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- 2021
30. Evaluation of two automated low-cost RNA extraction protocols for SARS-CoV-2 detection
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Fernando Lázaro Perona, Carlos Rodriguez-Antolin, Julio Garcia-Rodriguez, Carlos Toro, Emilio Cendejas-Bueno, Jesus Mingorance, and Marina Alguacil Guillén
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RNA viruses ,Computer science ,Coronaviruses ,Biochemistry ,chemistry.chemical_compound ,Nucleic Acids ,Pathology and laboratory medicine ,Virus Testing ,Multidisciplinary ,Organic Compounds ,Medical microbiology ,Viral Load ,Separation Processes ,Chemistry ,COVID-19 Nucleic Acid Testing ,Viruses ,Physical Sciences ,Costs and Cost Analysis ,Medicine ,RNA, Viral ,RNA extraction ,SARS CoV 2 ,Pathogens ,Research Article ,SARS coronavirus ,Science ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Commercial kit ,Microbiology ,Sensitivity and Specificity ,Viral genetics ,Extraction techniques ,Diagnostic Medicine ,Virology ,Viral Nucleic Acid ,Humans ,Medicine and health sciences ,Automation, Laboratory ,Chromatography ,Biology and life sciences ,Ethanol ,Elution ,Extraction (chemistry) ,Organic Chemistry ,Organisms ,Viral pathogens ,Chemical Compounds ,Viral Replication ,Microbial pathogens ,Research and analysis methods ,chemistry ,Alcohols ,Nucleic acid ,Reagent Kits, Diagnostic ,DNA ,Viral Transmission and Infection - Abstract
BackgroundTwo automatable in-house protocols for high-troughput RNA extraction from nasopharyngeal swabs for SARS-CoV-2 detection have been evaluated.MethodsOne hundred forty one SARS-CoV-2 positive samples were collected during a period of 10-days. In-house protocols were based on extraction with magnetic beads and designed to be used with either the Opentrons OT-2 (OT-2in-house) liquid handling robot or the MagMAXTMExpress-96 system (MMin-house). Both protocols were tested in parallel with a commercial kit that uses the MagMAXTMsystem (MMkit). Nucleic acid extraction efficiencies were calculated from a SARS-CoV-2 DNA positive control.ResultsNo significant differences were found between both in-house protocols and the commercial kit in their performance to detect positive samples. The MMkitwas the most efficient although the MMin-housepresented, in average, lower Cts than the other two. In-house protocols allowed to save between 350€ and 400€ for every 96 extracted samples compared to the commercial kit.ConclusionThe protocols described harness the use of easily available reagents and an open-source liquid handling system and are suitable for SARS-CoV-2 detection in high throughput facilities.
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- 2021
31. Inflammasome genes polymorphisms may influence the development of hepatitis C in the Amazonas, Brazil
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Toro, Diana Mota, primary, Ramasawmy, Rajendranath, additional, Silva Neto, Pedro Vieira, additional, Pereira, Grenda Leite, additional, Sarmento, Priscila Santos, additional, Dray, Hanna Lara Silva Negreiros, additional, Sousa, Keyla Santos, additional, Affonso, Juliana Santos, additional, Silva, Jéssica Albuquerque, additional, Garcia, Nadja Pinto, additional, Barbieri, Marilú Victória, additional, Victória, Flamir Silva, additional, Donadi, Eduardo Antônio, additional, Costa, Allyson Guimarães, additional, Ogusku, Mauricio Morishi, additional, Sadahiro, Aya, additional, Tarragô, Andréa Monteiro, additional, and Malheiro, Adriana, additional
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- 2021
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32. Understanding the complexity of disease-climate interactions for rice bacterial panicle blight under tropical conditions
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Echeverri-Rico, Johanna, primary, Petro, Eliel, additional, Fory, Paola A., additional, Mosquera, Gloria M., additional, Lang, Jillian M., additional, Leach, Jan E., additional, Lobaton, Juan D., additional, Garcés, Gabriel, additional, Perafán, Ricardo, additional, Amezquita, Nelson, additional, Toro, Shirley, additional, Mora, Brayan, additional, Cuasquer, Juan B., additional, Ramirez-Villegas, Julián, additional, Rebolledo, Maria Camila, additional, and Torres, Edgar A., additional
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- 2021
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33. Five-year follow-up of secondary iris-claw intraocular lens implantation for the treatment of aphakia: Anterior chamber versus retropupillary implantation
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Michele Figus, Michele Reibaldi, Sarah Tripodi, Mario Damiano Toro, Chiara Posarelli, Anselm Jünemann, Caterina Gagliano, Matteo Forlini, Tomasz Choragiewicz, Antonio Longo, Katarzyna Nowomiejska, Robert Rejdak, Teresio Avitabile, Agnieszka Kaminska, Toro, M. D., Longo, A., Avitabile, T., Nowomiejska, K., Gagliano, C., Tripodi, S., Choragiewicz, T., Kaminska, A., Figus, M., Posarelli, C., Forlini, M., Junemann, A. G. M., Reibaldi, M., and Rejdak, R.
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Genetics and Molecular Biology (all) ,Male ,genetic structures ,medicine.medical_treatment ,Iris ,Intraocular lens ,Ophthalmologic Surgical Procedures ,Eye ,Biochemistry ,law.invention ,Cornea ,Lens ,0302 clinical medicine ,Lense ,Randomized controlled trial ,Lens Implantation, Intraocular ,Biochemistry, Genetics and Molecular Biology (all) ,Agricultural and Biological Sciences (all) ,law ,Lenses ,Lenses, Intraocular ,0303 health sciences ,Endothelial Cell ,Multidisciplinary ,Intraocular ,medicine.anatomical_structure ,Iri ,Ophthalmologic Surgical Procedure ,Medicine ,Female ,Analysis of variance ,Lens Implantation ,Human ,Aged ,Anterior Chamber ,Aphakia ,Endothelial Cells ,Humans ,Lens, Crystalline ,medicine.medical_specialty ,Science ,03 medical and health sciences ,Ophthalmology ,medicine ,030304 developmental biology ,Crystalline ,business.industry ,Capsule ,Len ,Cataract surgery ,medicine.disease ,eye diseases ,030221 ophthalmology & optometry ,sense organs ,business - Abstract
BackgroundThough several procedures of IOL implantation have been described (sutured scleral fixation, intra-scleral fixation, angle-supported anterior chamber, and anterior chamber or retropupillary iris-claw IOLs), there are no randomized trials which are comparing different techniques. Hence, the surgical treatment of aphakia still remains controversial and challenging. The purpose of this study was to compare the long-term efficacy and the rate of complications of anterior versus posterior Iris-claw intraocular lenses (IOL) implantation to correct for the treatment of aphakia without sufficient capsule support.Methods and findingsConsecutive eyes having secondary implantation of aphakic iris-fixated IOLs with a follow-up of at least 5 years were considered. Mean correct distance visual acuity (CDVA) changes, percentage of eyes with CDVA improvement, mean corneal endothelial cell density (CECD) loss and the rate of other complications were used for statistical analysis. The study evaluated a total of 180 eyes (Group A: 87 anterior chamber iris-claw fixation, Group B: 93 retropupillary iris-claw implantation) of 180 consecutive different patients, with aphakia of various reasons. CDVA improved significantly in both groups after surgery (PConclusionsFive-year follow-up shows that secondary implantation of aphakic IOLs is effective and safe for the correction treatment of aphakia in eyes without capsule support.
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- 2019
34. Primary vitrectomy for degenerative and tractional lamellar macular holes: A systematic review and meta-analysis
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Parisi, Guglielmo, primary, Fallico, Matteo, additional, Maugeri, Andrea, additional, Barchitta, Martina, additional, Agodi, Antonella, additional, Russo, Andrea, additional, Longo, Antonio, additional, Avitabile, Teresio, additional, Castellino, Niccolò, additional, Bonfiglio, Vincenza, additional, Dell’Omo, Roberto, additional, Furino, Claudio, additional, Cennamo, Gilda, additional, Rejdak, Robert, additional, Nowomiejska, Katarzyna, additional, Toro, Mario, additional, Marolo, Paola, additional, Ventre, Luca, additional, and Reibaldi, Michele, additional
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- 2021
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35. Kinetic and static perimetry after 16 years and additional OCT-A analysis in eyes with long-lasting optic disc drusen
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Koman-Wierdak, Edyta, primary, Nowomiejska, Katarzyna, additional, Brzozowska, Agnieszka, additional, Nowakowska, Dominika, additional, Toro, Mario Damiano, additional, Bonfiglio, Vincenza, additional, Reibaldi, Michele, additional, Avitabile, Teresio, additional, and Rejdak, Robert, additional
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- 2021
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36. Socioeconomic determinants of hypertension and prehypertension in Peru: Evidence from the Peruvian Demographic and Health Survey
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Chambergo-Michilot, Diego, primary, Rebatta-Acuña, Alexis, additional, Delgado-Flores, Carolina J., additional, and Toro-Huamanchumo, Carlos J., additional
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- 2021
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37. Socioeconomic determinants of hypertension and prehypertension in Peru: Evidence from the Peruvian Demographic and Health Survey
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Alexis Rebatta-Acuña, Carlos J. Toro-Huamanchumo, Diego Chambergo-Michilot, and Carolina J. Delgado-Flores
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Male ,Rural Population ,Urban Population ,Economics ,Physiology ,Social Sciences ,Blood Pressure ,Comorbidity ,Vascular Medicine ,Geographical locations ,Prehypertension ,Sociology ,Peru ,Medicine and Health Sciences ,Public and Occupational Health ,Abdominal obesity ,Geographic Areas ,education.field_of_study ,Multidisciplinary ,Geography ,Middle Aged ,Socioeconomic Aspects of Health ,Physiological Parameters ,Hypertension ,Medicine ,Marital status ,Female ,medicine.symptom ,Research Article ,Urban Areas ,Adult ,Adolescent ,Science ,Population ,Human Geography ,Education ,Urban Geography ,Health Economics ,Environmental health ,medicine ,Humans ,Obesity ,education ,Socioeconomic status ,Educational Attainment ,Aged ,business.industry ,Body Weight ,Biology and Life Sciences ,South America ,medicine.disease ,Health Surveys ,Health Care ,Blood pressure ,Socioeconomic Factors ,Earth Sciences ,Rural area ,People and places ,business ,Health Insurance - Abstract
Background Peru is a Latin American country with a significant burden of hypertension that presents worrying rates of disparities in socioeconomic determinants. However, there is a lack of studies exploring the association between those determinants, hypertension and prehypertension in Peruvian population. Objective We aimed to assess the association betwgeen socioeconomic determinants, hypertension and prehypertension using a nationally representative survey of Peruvians. Methods We performed a cross-sectional analysis of the Peruvian Demographic and Health Survey (2018), which is a two-staged regional-level representative survey. We used data from 33,336 people aged 15 and older. The dependent variable was blood pressure classification (normal, prehypertension and hypertension) following the Seventh Report of the Joint National Committee (JNC-7) on hypertension management. Independent variables were socioeconomic: age, sex, marital status, wealth index, health insurance, education, region and area of residence. Due to the nature of the dependent variable (more than two categories), we opted to use the multinomial regression model, adjusting the effect of the multistage sample using the svy command. We tested interactions with the adjusted Wald test. Results The prevalence of prehypertension and hypertension was 33.68% and 19.77%, respectively. Awareness was higher in urban than in rural areas (9.61% vs. 8.31%, p = 0.008). Factors associated with a higher prevalence ratio of both prehypertension and hypertension were age (ratios rose with each age group), male sex (prehypertension aRPR 5.15, 95%CI 4.63–5.73; hypertension aRPR 3.85, 95% CI 3.37–4.40) and abdominal obesity (prehypertension aRPR 2.11, 95%CI 1.92–2.31; hypertension aRPR 3.04, 95% CI 2.69–3.43). Factors with a lower prevalence ratio of both diseases were secondary education (prehypertension aRPR 0.76, 95%CI 0.60–0.95; hypertension aRPR 0.75, 95% CI 0.58–0.97), higher education (prehypertension aRPR 0.78, 95%CI 0.61–0.99; hypertension aRPR 0.62, 95% CI 0.46–0.82), being married/cohabiting (prehypertension aRPR 0.87, 95%CI 0.79–0.95; hypertension aRPR 0.77, 95% CI 0.68–0.87), richest wealth index (only prehypertension aRPR 0.76, 95%CI 0.63–0.92) and living in cities different to Lima (rest of the Coastline, Highlands and Jungle). Having health insurance (only hypertension aRPR 1.26, 95%CI 1.03–1.53) and current drinking (only prehypertension aRPR 1.15, 95%CI 1.01–1.32) became significant factors in rural areas. Conclusions We evidenced socioeconomic disparities among people with hypertension and prehypertension. Better health policies on reducing the burden of risk factors are needed, besides, policy decision makers should focus on hypertension preventive strategies in Peru.
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- 2020
38. Preliminary steps of the development of a Minimum Uniform Dataset applicable to the international wheelchair sector
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Padmaja Kankipati, Nancy Augustine, Karen Rispin, Richard M. Schein, Krithika Kandavel, Patricia Karg, María Luisa Toro-Hernández, Megan E. D’Innocenzo, Mary Goldberg, and Jonathan Pearlman
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Questionnaires ,030506 rehabilitation ,Computer science ,Economics ,Social Sciences ,Transportation ,Economic Geography ,Surveys ,Global Health ,0302 clinical medicine ,Wheelchair ,Sociology ,Surveys and Questionnaires ,Task Performance and Analysis ,Medicine and Health Sciences ,Public and Occupational Health ,030212 general & internal medicine ,Data Management ,Multidisciplinary ,Schools ,Geography ,Data Collection ,Investment (macroeconomics) ,Work (electrical) ,Research Design ,Medicine ,Engineering and Technology ,Low and Middle Income Countries ,0305 other medical science ,Research Article ,Biotechnology ,Computer and Information Sciences ,Science ,Bioengineering ,Research and Analysis Methods ,Education ,03 medical and health sciences ,Humans ,Disabled Persons ,Survey Research ,International Agencies ,Biology and Life Sciences ,Translating ,Engineering management ,Assistive Technologies ,Wheelchairs ,Earth Sciences ,Medical Devices and Equipment - Abstract
Assistive products outcomes are needed globally to inform policy, practice, and drive investment. The International Society of Wheelchair Professionals developed a Minimum Uniform Dataset (MUD) for wheelchair services worldwide with the intent to gather data that is comparable globally. The MUD was developed with the participation of members from around the globe and its feasibility piloted at 3 sites. Three versions of the MUD are now available-a short form with 29 data points (available in English, Spanish, and French) and a standard version with 38 data points in English. Future work is to validate and complete the translation cycles followed by promoting the use of the MUD globally so that the data can be leveraged to inform policy, practice and direct investments.
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- 2020
39. Optimization of convolutional neural network hyperparameters for automatic classification of adult mosquitoes
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Matias Valdenegro-Toro, Bruna Aparecida Souza Machado, Otavio Gonçalvez Vicente Ribeiro-Filho, Alex Álisson Bandeira Santos, Roberto Badaró, Luis Octavio Arriaga Camargo, Frank Kirchner, and Daniel Motta
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0301 basic medicine ,Male ,Computer science ,Entropy ,Overfitting ,Disease Vectors ,medicine.disease_cause ,computer.software_genre ,Convolutional neural network ,Mosquitoes ,Dengue fever ,Dengue ,0302 clinical medicine ,Aedes ,Medicine and Health Sciences ,Image Processing, Computer-Assisted ,Chikungunya ,Multidisciplinary ,biology ,Artificial neural network ,Zika Virus Infection ,Physics ,Eukaryota ,Insects ,Culex ,Infectious Diseases ,Physical Sciences ,Viruses ,Medicine ,Thermodynamics ,Female ,Chikungunya virus ,Research Article ,Computer and Information Sciences ,Aedes albopictus ,Arthropoda ,Neural Networks ,Infectious Disease Control ,Science ,030231 tropical medicine ,Aedes aegypti ,Mosquito Vectors ,Aedes Aegypti ,Machine learning ,03 medical and health sciences ,parasitic diseases ,medicine ,Animals ,Automation, Laboratory ,business.industry ,fungi ,Organisms ,Biology and Life Sciences ,Zika Virus ,Dengue Virus ,medicine.disease ,biology.organism_classification ,Invertebrates ,Culex quinquefasciatus ,Insect Vectors ,Species Interactions ,030104 developmental biology ,Culicidae ,Chikungunya Fever ,Artificial intelligence ,business ,computer ,Zoology ,Entomology ,Arboviruses ,Neuroscience - Abstract
The economic and social impacts due to diseases transmitted by mosquitoes in the latest years have been significant. Currently, no specific treatment or commercial vaccine exists for the control and prevention of arboviruses, thereby making entomological characterization fundamental in combating diseases such as dengue, chikungunya, and Zika. The morphological identification of mosquitos includes a visual exam of the samples. It is time consuming and requires adequately trained professionals. Accordingly, the development of a new automated method for realizing mosquito-perception and -classification is becoming increasingly essential. Therefore, in this study, a computational model based on a convolutional neural network (CNN) was developed to extract features from the images of mosquitoes and then classify the species Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus. In addition, the model was trained to detect the mosquitoes of the genus Aedes. To train CNNs to perform the automatic morphological classification of mosquitoes, a dataset, which included 7,561 images of the target mosquitoes and 1,187 images of other insects, was acquired. Various neural networks, such as Xception and DenseNet, were used for developing the automatic-classification model based on images. A structured optimization process of random search and grid search was developed to select the hyperparameters set and increase the accuracy of the model. In addition, strategies to eliminate overfitting were implemented to increase the generalization of the model. The optimized model, during the test phase, obtained the balanced accuracy (BA) of 93.5% in classifying the target mosquitoes and other insects and the BA of 97.3% in detecting the mosquitoes of the genus Aedes in comparison to Culex. The results provide fundamental information for performing the automatic morphological classification of mosquito species. Using a CNN-embedded entomological tool is a valuable and accessible resource for health workers and non-taxonomists for identifying insects that can transmit infectious diseases.
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- 2020
40. Subspheroids in the lithic assemblage of Barranco León (Spain): Recognizing the late Oldowan in Europe
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Juan Manuel Jiménez Arenas, Alexia Serrano-Ramos, Robert Sala-Ramos, Amèlia Bargalló, Josep Maria Vergès, Isidro Toro-Moyano, Stefania Titton, Deborah Barsky, and José Antonio García Solano
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Hominids ,Raw Materials ,Culture ,Social Sciences ,Phase Determination ,01 natural sciences ,Scraper site ,Sociology ,Assemblage (archaeology) ,0601 history and archaeology ,Materials ,History, Ancient ,Stone tool ,Minerals ,Multidisciplinary ,060102 archaeology ,Knapping ,Fossils ,Hominidae ,06 humanities and the arts ,Mineralogy ,Limestone ,Geography ,Archaeology ,Physical Sciences ,Crystallographic Techniques ,Medicine ,Physical Anthropology ,Research Article ,010506 paleontology ,Science ,Materials Science ,Context (language use) ,engineering.material ,Research and Analysis Methods ,Archaic Humans ,Lithic technology ,Cultural Evolution ,Paleoanthropology ,Animals ,Hominins ,0105 earth and related environmental sciences ,Tool Use Behavior ,Correction ,Biology and Life Sciences ,Paleontology ,Lithic Technology ,Spain ,Anthropology ,Earth Sciences ,engineering ,Oldowan - Abstract
All relevant data are within the manuscript and its Supporting Information files. Lithic material inventories and basic analyses may be consulted in the Field Season Reports that the Orce Research Team delivers yearly to Junta de Andalucia, which are freely available by request. The studied material is preserved in the Archaeological and Ethnographical Museum of Granada (Andalucía, Spain)., The lithic assemblage of Barranco León (BL), attributed to the Oldowan techno-complex, contributes valuable information to reconstruct behavioral patterning of the first hominins to disperse into Western Europe. This archaic stone tool assemblage comprises two, very different groups of tools, made from distinct raw materials. On the one hand, a small-sized toolkit knapped from Jurassic flint, comprising intensively exploited cores and small-sized flakes and fragments and, on the other hand, a large-sized limestone toolkit that is mainly linked to percussive activities. In recent years, the limestone macro-tools have been the center of particular attention, leading to a re-evaluation of their role in the assemblage. Main results bring to light strict hominin selective processes, mainly concerning the quality of the limestone and the morphology of the cobbles, in relation to their use-patterning. In addition to the variety of traces of percussion identified on the limestone tools, recurrences have recently been documented in their positioning and in the morphology of the active surfaces. Coupled with experimental work, this data has contributed to formulating hypothesis about the range of uses for these tools, beyond stone knapping and butchery, for activities such as: wood-working or tendon and meat tenderizing. The abundance of hammerstones, as well as the presence of heavy-duty scrapers, are special features recognized for the limestone component of the Barranco Leo´n assemblage. This paper presents, for the first time, another characteristic of the assemblage: the presence of polyhedral and, especially, subspheroid morphologies, virtually unknown in the European context for this timeframe. We present an analysis of these tools, combining qualitative evaluation of the raw materials, diacritical study, 3D geometric morphometric analysis of facet angles and an evaluation of the type and position of percussive traces; opening up the discussion of the late Oldowan beyond the African context., This research has been funded by the Junta de Andalucía, Consejería de Educación, Cultura y Deporte: Orce Research Project “Primeras ocupaciones humanas y contexto paleoecológico a partir de los depósitos pliopleistocenos de la cuenca Guadix-Baza: zona arqueolo´gica de la Cuenca de Orce (Granada, España), 2017–2020”; "Presencia humana y contexto paleoecológico en la cuenca continental de Guadix-Baza. Estudio e interpretacio´n a partir de los depósitos Plio-Pleistocénicos de Orce. Granada. España" B120489SV18BC, 2012-16; "Primeras ocupaciones humanas del Pleistoceno inferior de la cuenca de Guadix-Baza (Granada, España)" B090678SVI8BC, 2009-11; MICINN (no feder) "Estudio de las dispersiones faunísticas y humanas durante el Pleistoceno inferior en la cuenca mediterránea.", CGL2016-80975-P, 2017- 19; the Spanish government Ministerio de Ciencia, Innovación y Universidades (MICINN-FEDER) code CGL2016-80975-P, and the Generalitat de Catalunya Research Group 2017SGR 859. “Comportamiento ecosocial de los homínidos de la Sierra de Atapuerca durante el Cuaternario V”, MICINN-FEDER PGC2018-093925-B-C32 and the Generalitat de Catalunya, AGAUR agency, SGR 859 and SGR 1040. Gerda Henkel Foundation (AZ 32/V/ 19, Lower Paleolithic Spheroids Project (LPSP) is assuring continuity in this line of research. ST is beneficiary of the Provincia Autonoma di Bolzano (Italy) post-master scholarship. AB has been funded from the European Union’s Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie Action grant agreement PREKARN nº702584. The research of DB, JMV, & RSR is funded by CERCA Programme/Generalitat de Catalunya. JMJA belongs to the Research Group HUM-607.
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- 2020
41. Preliminary steps of the development of a Minimum Uniform Dataset applicable to the international wheelchair sector
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María Luisa Toro-Hernández, Nancy Augustine, Padmaja Kankipati, Patricia Karg, Karen Rispin, Richard M. Schein, Krithika Kandavel, Megan E. D’Innocenzo, Mary Goldberg, Jonathan Pearlman, and Sara Rubinelli
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lcsh:R ,lcsh:Medicine ,lcsh:Q ,lcsh:Science - Abstract
Assistive products outcomes are needed globally to inform policy, practice, and drive investment. The International Society of Wheelchair Professionals developed a Minimum Uniform Dataset (MUD) for wheelchair services worldwide with the intent to gather data that is comparable globally. The MUD was developed with the participation of members from around the globe and its feasibility piloted at 3 sites. Three versions of the MUD are now available—a short form with 29 data points (available in English, Spanish, and French) and a standard version with 38 data points in English. Future work is to validate and complete the translation cycles followed by promoting the use of the MUD globally so that the data can be leveraged to inform policy, practice and direct investments.
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- 2020
42. A new pharmacodynamic approach to study antibiotic combinations against enterococci in vivo: Application to ampicillin plus ceftriaxone
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Jimenez-Toro, Ivone, primary, Rodriguez, Carlos A., additional, Zuluaga, Andres F., additional, Otalvaro, Julian D., additional, and Vesga, Omar, additional
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- 2020
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43. Forest canopy-cover composition and landscape influence on bryophyte communities in Nothofagus forests of southern Patagonia
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Toro Manríquez, Mónica D. R., primary, Ardiles, Víctor, additional, Promis, Álvaro, additional, Huertas Herrera, Alejandro, additional, Soler, Rosina, additional, Lencinas, María Vanessa, additional, and Martínez Pastur, Guillermo, additional
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- 2020
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44. SUMO conjugation susceptibility of Akt/protein kinase B affects the expression of the pluripotency transcription factor Nanog in embryonic stem cells
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Marcos Francia, Martin Stortz, Santiago Gabriel Miriuka, Ayelén Toro, Paula Verneri, Camila Vazquez Echegaray, Alejandra Guberman, Camila Oses, María Soledad Cosentino, Ariel Waisman, Valeria Levi, and María Victoria Petrone
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0301 basic medicine ,Carcinogenesis ,SUMO protein ,Gene Expression ,AKT1 ,Immunostaining ,Biochemistry ,0302 clinical medicine ,Animal Cells ,Medicine and Health Sciences ,Staining ,Regulation of gene expression ,Multidisciplinary ,Kinase ,Chemistry ,Stem Cells ,Enzymes ,Cell biology ,Oncology ,030220 oncology & carcinogenesis ,embryonic structures ,Medicine ,Post-translational modification ,Cellular Types ,Stem cell ,Oxidoreductases ,Luciferase ,Research Article ,Pluripotency ,Homeobox protein NANOG ,Science ,Cell Potency ,Transfection ,Research and Analysis Methods ,03 medical and health sciences ,Genetics ,Animals ,Gene Regulation ,Molecular Biology Techniques ,Molecular Biology ,Protein kinase B ,Transcription factor ,Embryonic Stem Cells ,Ubiquitin ,Sumoylation ,Biology and Life Sciences ,Proteins ,Cell Biology ,030104 developmental biology ,Specimen Preparation and Treatment ,Enzymology ,Proto-Oncogene Proteins c-akt - Abstract
Akt/PKB is a kinase involved in the regulation of a wide variety of cell processes. Its activity is modulated by diverse post-translational modifications (PTMs). Particularly, conjugation of the small ubiquitin-related modifier (SUMO) to this kinase impacts on multiple cellular functions, such as proliferation and splicing. In embryonic stem (ES) cells, this kinase is key for pluripotency maintenance. Among other functions, Akt is known to promote the expression of Nanog, a central pluripotency transcription factor (TF). However, the relevance of this specific PTM of Akt has not been previously analyzed in this context. In this work, we study the effect of Akt1 variants with differential SUMOylation susceptibility on the expression of Nanog. Our results demonstrate that both, the Akt1 capability of being modified by SUMO conjugation and a functional SUMO conjugase activity are required to induce Nanog gene expression. Likewise, we found that the common oncogenic E17K Akt1 mutant affected Nanog expression in ES cells also in a SUMOylatability dependent manner. Interestingly, this outcome takes places in ES cells but not in a non-pluripotent heterologous system, suggesting the presence of a crucial factor for this induction in ES cells. Remarkably, the two major candidate factors to mediate this induction, GSK3-β and Tbx3, are non-essential players of this effect, suggesting a complex mechanism probably involving non-canonical pathways. Furthermore, we found that Akt1 subcellular distribution does not depend on its SUMOylatability, indicating that Akt localization has no influence on the effect on Nanog, and that besides the membrane localization of E17K Akt mutant, SUMOylation is also required for its hyperactivity. Our results highlight the impact of SUMO conjugation in the function of a kinase relevant for a plethora of cellular processes, including the control of a key pluripotency TF.
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- 2021
45. Preliminary steps of the development of a Minimum Uniform Dataset applicable to the international wheelchair sector
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Toro-Hernández, María Luisa, primary, Augustine, Nancy, additional, Kankipati, Padmaja, additional, Karg, Patricia, additional, Rispin, Karen, additional, Schein, Richard M., additional, Kandavel, Krithika, additional, D’Innocenzo, Megan E., additional, Goldberg, Mary, additional, and Pearlman, Jonathan, additional
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- 2020
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46. A high-throughput imaging and quantification pipeline for the EVOS imaging platform
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Klimaj, Stefan Donovan, primary, Licon Munoz, Yamhilette, additional, Del Toro, Katelyn, additional, and Hines, William Curtis, additional
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- 2020
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47. Optimization of convolutional neural network hyperparameters for automatic classification of adult mosquitoes
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Motta, Daniel, primary, Santos, Alex Álisson Bandeira, additional, Machado, Bruna Aparecida Souza, additional, Ribeiro-Filho, Otavio Gonçalvez Vicente, additional, Camargo, Luis Octavio Arriaga, additional, Valdenegro-Toro, Matias Alejandro, additional, Kirchner, Frank, additional, and Badaró, Roberto, additional
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- 2020
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48. Mapping the status of the North American beaver invasion in the Tierra del Fuego archipelago
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Huertas Herrera, Alejandro, primary, Lencinas, María Vanessa, additional, Toro Manríquez, Mónica, additional, Miller, Juan Andrés, additional, and Martínez Pastur, Guillermo, additional
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- 2020
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49. Agroecosystem resilience. A conceptual and methodological framework for evaluation
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Córdoba, Cindy, primary, Triviño, Catalina, additional, and Toro Calderón, Javier, additional
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- 2020
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50. Global drivers of food system (un)sustainability: A multi-country correlation analysis
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Béné, Christophe, primary, Fanzo, Jessica, additional, Prager, Steven D., additional, Achicanoy, Harold A., additional, Mapes, Brendan R., additional, Alvarez Toro, Patricia, additional, and Bonilla Cedrez, Camila, additional
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- 2020
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