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16 results on '"Sun, Ya"'

2. Global socio-economic losses and environmental gains from the Coronavirus pandemic

Author

Lenzen, Manfred, primary, Li, Mengyu, additional, Malik, Arunima, additional, Pomponi, Francesco, additional, Sun, Ya-Yen, additional, Wiedmann, Thomas, additional, Faturay, Futu, additional, Fry, Jacob, additional, Gallego, Blanca, additional, Geschke, Arne, additional, Gómez-Paredes, Jorge, additional, Kanemoto, Keiichiro, additional, Kenway, Steven, additional, Nansai, Keisuke, additional, Prokopenko, Mikhail, additional, Wakiyama, Takako, additional, Wang, Yafei, additional, and Yousefzadeh, Moslem, additional

Published
2020
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6. Selection of diethylstilbestrol-specific single-chain antibodies from a non-immunized mouse ribosome display library

Author

Baoan Ning, Xianjun Fan, Sun Ya Nan, Xianjun Gao, Gao Zhixian, Ming Liu, and Jianqing Liu

Subjects
Agricultural Biotechnology, lcsh:Medicine, medicine.disease_cause, Biochemistry, Mice, Antibody Specificity, lcsh:Science, Peptide sequence, Animal Management, Electrophoresis, Agar Gel, Immunoassay, Multidisciplinary, Expression vector, Agriculture, Translation (biology), Chemistry, Environmental Pollutants, Female, Agrochemicals, Research Article, Biotechnology, Macromolecular Substances, Sequence analysis, Genetic Vectors, Molecular Sequence Data, Enzyme-Linked Immunosorbent Assay, Biology, Agricultural Production, Peptide Library, Escherichia coli, medicine, Environmental Chemistry, Animals, Amino Acid Sequence, Estrogens, Non-Steroidal, Peptide library, Diethylstilbestrol, Base Sequence, lcsh:R, Correction, Sequence Analysis, DNA, Surface Plasmon Resonance, Molecular biology, Ribosome display, lcsh:Q, Ribosomes, Spleen, Single-Chain Antibodies
Abstract

Single chain variable fragments (scFvs) against diethylstilbestrol (DES) were selected from the splenocytes of non-immunized mice by ribosome display technology. A naive library was constructed and engineered to allow in vitro transcription and translation using an E. coli lysate system. Alternating selection in solution and immobilization in microtiter wells was used to pan mRNA-ribosome-antibody (ARM) complexes. After seven rounds of ribosome display, the expression vector pTIG-TRX containing the selected specific scFv DNAs were transformed into Escherichia coli BL21 (DE3) for expression. Twenty-six positive clones were screened and five clones had high antibody affinity and specificity to DES as evidenced by indirect competitive ELISA. Sequence analysis showed that these five DES-specific scFvs had different amino acid sequences, but the CDRs were highly similar. Surface plasmon resonance (SPR) analysis was used to determine binding kinetics of one clone (30-1). The measured K(D) was 3.79 µM. These results indicate that ribosome display technology can be used to efficiently isolate hapten-specific antibody (Ab) fragments from a naive library; this study provides a methodological framework for the development of novel immunoassays for multiple environmental pollutants with low molecular weight detection using recombinant antibodies.

Published
2012

16. Impact of HIV on cell survival and antiviral activity of plasmacytoid dendritic cells.

Author

Meyers JH, Justement JS, Hallahan CW, Blair ET, Sun YA, O'Shea MA, Roby G, Kottilil S, Moir S, Kovacs CM, Chun TW, and Fauci AS

Subjects
Dendritic Cells immunology, Gene Expression Profiling, HIV Infections immunology, Humans, Cell Survival physiology, Dendritic Cells physiology, HIV physiology, Virus Replication immunology
Abstract

Plasmacytoid dendritic cells (pDCs) are important mediators of innate immunity that act mainly through secretion of interferon (IFN)-alpha. Previous studies have found that these cells can suppress HIV in vitro; additionally, pDCs have been shown to be severely reduced in the peripheral blood of HIV-infected individuals. In the present study, we sought to determine the ability of pDCs to directly suppress viral replication ex vivo and to delineate the potential mechanisms whereby pDCs are depleted in HIV-infected individuals. We demonstrate that activated pDCs strongly suppress HIV replication in autologous CD4(+) T cells via a mechanism involving IFN-alpha as well as other antiviral factors. Of note, unstimulated pDCs from infected individuals who maintain low levels of plasma viremia without antiretroviral therapy were able to suppress HIV ex vivo via a mechanism requiring cell-to-cell contact. Our data also demonstrate that death of pDCs by both apoptosis and necrosis is induced by fusion of HIV with pDCs. Taken together, our data suggest that pDCs play an important role in the control of HIV replication and that high levels of viral replication in vivo are associated with pDC cell death via apoptosis and necrosis. Elucidation of the mechanism by which pDCs suppress HIV replication in vivo may have clinically relevant implications for future therapeutic strategies.

Published
2007
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