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1. The non-classical MAP kinase ERK3 controls T cell activation.

Author

Miriam Marquis, Salix Boulet, Simon Mathien, Justine Rousseau, Paméla Thébault, Jean-François Daudelin, Julie Rooney, Benjamin Turgeon, Claudine Beauchamp, Sylvain Meloche, and Nathalie Labrecque

Subjects
Medicine, Science
Abstract

The classical mitogen-activated protein kinases (MAPKs) ERK1 and ERK2 are activated upon stimulation of cells with a broad range of extracellular signals (including antigens) allowing cellular responses to occur. ERK3 is an atypical member of the MAPK family with highest homology to ERK1/2. Therefore, we evaluated the role of ERK3 in mature T cell response. Mouse resting T cells do not transcribe ERK3 but its expression is induced in both CD4⁺ and CD8⁺ T cells following T cell receptor (TCR)-induced T cell activation. This induction of ERK3 expression in T lymphocytes requires activation of the classical MAPK ERK1 and ERK2. Moreover, ERK3 protein is phosphorylated and associates with MK5 in activated primary T cells. We show that ERK3-deficient T cells have a decreased proliferation rate and are impaired in cytokine secretion following in vitro stimulation with low dose of anti-CD3 antibodies. Our findings identify the atypical MAPK ERK3 as a new and important regulator of TCR-induced T cell activation.

Published
2014
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2. The non-classical MAP kinase ERK3 controls T cell activation

Author

Claudine Beauchamp, Benjamin Turgeon, Justine Rousseau, Julie Rooney, Salix Boulet, Nathalie Labrecque, Jean-François Daudelin, Sylvain Meloche, Simon Mathien, Miriam Marquis, and Pamela Thebault

Subjects
MAPK signaling cascades, T cell, Immune Cells, T-Lymphocytes, Immunology, Immunoblotting, lcsh:Medicine, Biology, Signal transduction, ERK signaling cascade, Lymphocyte Activation, Interleukin 21, Mice, Molecular cell biology, medicine, Cytotoxic T cell, Animals, Immunoprecipitation, IL-2 receptor, Antigen-presenting cell, lcsh:Science, Immune Response, Cell Proliferation, DNA Primers, Mitogen-Activated Protein Kinase 6, Immunity, Cellular, Multidisciplinary, T Cells, Reverse Transcriptase Polymerase Chain Reaction, ZAP70, lcsh:R, CD28, Signaling cascades, Natural killer T cell, Flow Cytometry, beta-Galactosidase, Molecular biology, Cell biology, medicine.anatomical_structure, Gene Expression Regulation, Medicine, Cytokines, Clinical Immunology, lcsh:Q, Research Article
Abstract

The classical mitogen-activated protein kinases (MAPKs) ERK1 and ERK2 are activated upon stimulation of cells with a broad range of extracellular signals (including antigens) allowing cellular responses to occur. ERK3 is an atypical member of the MAPK family with highest homology to ERK1/2. Therefore, we evaluated the role of ERK3 in mature T cell response. Mouse resting T cells do not transcribe ERK3 but its expression is induced in both CD4+ and CD8+ T cells following T cell receptor (TCR)-induced T cell activation. This induction of ERK3 expression in T lymphocytes requires activation of the classical MAPK ERK1 and ERK2. Moreover, ERK3 protein is phosphorylated and associates with MK5 in activated primary T cells. We show that ERK3-deficient T cells have a decreased proliferation rate and are impaired in cytokine secretion following in vitro stimulation with low dose of anti-CD3 antibodies. Our findings identify the atypical MAPK ERK3 as a new and important regulator of TCR-induced T cell activation.

Published
2014

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