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1. Titanium Surface Priming with Phase-Transited Lysozyme to Establish a Silver Nanoparticle-Loaded Chitosan/Hyaluronic Acid Antibacterial Multilayer via Layer-by-Layer Self-Assembly.

Author

Xue Zhong, Yunjia Song, Peng Yang, Yao Wang, Shaoyun Jiang, Xu Zhang, and Changyi Li

Subjects
Medicine, Science
Abstract

The formation of biofilm around implants, which is induced by immediate bacterial colonization after installation, is the primary cause of post-operation infection. Initial surface modification is usually required to incorporate antibacterial agents on titanium (Ti) surfaces to inhibit biofilm formation. However, simple and effective priming methods are still lacking for the development of an initial functional layer as a base for subsequent coatings on titanium surfaces. The purpose of our work was to establish a novel initial layer on Ti surfaces using phase-transited lysozyme (PTL), on which multilayer coatings can incorporate silver nanoparticles (AgNP) using chitosan (CS) and hyaluronic acid (HA) via a layer-by-layer (LbL) self-assembly technique.In this study, the surfaces of Ti substrates were primed by dipping into a mixture of lysozyme and tris(2-carboxyethyl)phosphine (TCEP) to obtain PTL-functionalized Ti substrates. The subsequent alternating coatings of HA and chitosan loaded with AgNP onto the precursor layer of PTL were carried out via LbL self-assembly to construct multilayer coatings on Ti substrates.The results of SEM and XPS indicated that the necklace-like PTL and self-assembled multilayer were successfully immobilized on the Ti substrates. The multilayer coatings loaded with AgNP can kill planktonic and adherent bacteria to 100% during the first 4 days. The antibacterial efficacy of the samples against planktonic and adherent bacteria achieved 65%-90% after 14 days. The sustained release of Ag over 14 days can prevent bacterial invasion until mucosa healing. Although the AgNP-containing structure showed some cytotoxicity, the toxicity can be reduced by controlling the Ag release rate and concentration.The PTL priming method provides a promising strategy for fabricating long-term antibacterial multilayer coatings on titanium surfaces via the LbL self-assembly technique, which is effective in preventing implant-associated infections in the early stage.

Published
2016
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2. Titanium Surface Priming with Phase-Transited Lysozyme to Establish a Silver Nanoparticle-Loaded Chitosan/Hyaluronic Acid Antibacterial Multilayer via Layer-by-Layer Self-Assembly

Author

Peng Yang, Yao Wang, Xu Zhang, Shaoyun Jiang, Changyi Li, Xue Zhong, and Yunjia Song

Subjects
Cytotoxicity, Metal Nanoparticles, lcsh:Medicine, 02 engineering and technology, Toxicology, Pathology and Laboratory Medicine, 01 natural sciences, Silver nanoparticle, Chitosan, Mice, chemistry.chemical_compound, Spectrum Analysis Techniques, Coated Materials, Biocompatible, Hyaluronic acid, Medicine and Health Sciences, Nanotechnology, Hyaluronic Acid, lcsh:Science, Thin Films, Titanium, Multidisciplinary, Antimicrobials, Drugs, 021001 nanoscience & nanotechnology, Anti-Bacterial Agents, Chemistry, Physical Sciences, Engineering and Technology, Lysozyme, 0210 nano-technology, Layer (electronics), Research Article, Chemical Elements, Silver, Materials science, Materials by Structure, Cell Survival, Surface Properties, Materials Science, chemistry.chemical_element, Research and Analysis Methods, 010402 general chemistry, Microbiology, Cell Line, Coatings, Microbial Control, Animals, Materials by Attribute, Pharmacology, Dental Implants, Surface Treatments, lcsh:R, Biofilm, Biology and Life Sciences, Bacteriology, X-Ray Photoelectron Spectroscopy, 0104 chemical sciences, Manufacturing Processes, chemistry, Chemical engineering, Biofilms, Antibacterials, Nanoparticles, Surface modification, lcsh:Q, Bacterial Biofilms, Electron Beam Spectrum Analysis Techniques
Abstract

Objectives The formation of biofilm around implants, which is induced by immediate bacterial colonization after installation, is the primary cause of post-operation infection. Initial surface modification is usually required to incorporate antibacterial agents on titanium (Ti) surfaces to inhibit biofilm formation. However, simple and effective priming methods are still lacking for the development of an initial functional layer as a base for subsequent coatings on titanium surfaces. The purpose of our work was to establish a novel initial layer on Ti surfaces using phase-transited lysozyme (PTL), on which multilayer coatings can incorporate silver nanoparticles (AgNP) using chitosan (CS) and hyaluronic acid (HA) via a layer-by-layer (LbL) self-assembly technique. Methods In this study, the surfaces of Ti substrates were primed by dipping into a mixture of lysozyme and tris(2-carboxyethyl)phosphine (TCEP) to obtain PTL-functionalized Ti substrates. The subsequent alternating coatings of HA and chitosan loaded with AgNP onto the precursor layer of PTL were carried out via LbL self-assembly to construct multilayer coatings on Ti substrates. Results The results of SEM and XPS indicated that the necklace-like PTL and self-assembled multilayer were successfully immobilized on the Ti substrates. The multilayer coatings loaded with AgNP can kill planktonic and adherent bacteria to 100% during the first 4 days. The antibacterial efficacy of the samples against planktonic and adherent bacteria achieved 65%-90% after 14 days. The sustained release of Ag over 14 days can prevent bacterial invasion until mucosa healing. Although the AgNP-containing structure showed some cytotoxicity, the toxicity can be reduced by controlling the Ag release rate and concentration. Conclusions The PTL priming method provides a promising strategy for fabricating long-term antibacterial multilayer coatings on titanium surfaces via the LbL self-assembly technique, which is effective in preventing implant-associated infections in the early stage.

Published
2016

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