1. Offspring sex impacts DNA methylation and gene expression in placentae from women with diabetes during pregnancy
- Author
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April M. Teague, Yuet-Kin Leung, Sara E. Pinney, Steven D. Chernausek, Jing Chen, Christopher E. Aston, Jacqueline Alexander, and Rebecca A. Simmons
- Subjects
0301 basic medicine ,Male ,Proteomics ,Embryology ,Placenta ,Maternal Health ,Pregnancy in Diabetics ,Gene Expression ,lcsh:Medicine ,Biochemistry ,Endocrinology ,Pregnancy ,Gene expression ,Medicine and Health Sciences ,lcsh:Science ,Multidisciplinary ,DNA methylation ,Messenger RNA ,Chemical Reactions ,Obstetrics and Gynecology ,Methylation ,Chromatin ,Nucleic acids ,Chemistry ,CpG site ,Prenatal Exposure Delayed Effects ,Physical Sciences ,Female ,Epigenetics ,Anatomy ,DNA modification ,Chromatin modification ,Research Article ,Chromosome biology ,Adult ,Cell biology ,DNA repair ,Offspring ,Endocrine Disorders ,Biology ,Andrology ,03 medical and health sciences ,Young Adult ,Sex Factors ,Genetics ,Diabetes Mellitus ,Humans ,Obesity ,RNA, Messenger ,Gene ,Biology and life sciences ,lcsh:R ,Infant, Newborn ,Reproductive System ,RNA ,DNA ,030104 developmental biology ,Diabetes Mellitus, Type 2 ,Case-Control Studies ,Metabolic Disorders ,Women's Health ,CpG Islands ,lcsh:Q ,Protein Abundance ,Developmental Biology - Abstract
AIMS/HYPOTHESIS We hypothesized that diabetes during pregnancy (DDP) alters genome-wide DNA methylation in placenta resulting in differentially methylated loci of metabolically relevant genes and downstream changes in RNA and protein expression. METHODS We mapped genome-wide DNA methylation with the Infinium 450K Human Methylation Bead Chip in term fetal placentae from Native American and Hispanic women with DDP using a nested case-control design (n = 17 pairs). RNA expression and protein levels were assayed via RNA-Seq and Western Blot. RESULTS Genome-wide DNA methylation analysis revealed 465 CpG sites with significant changes for male offspring, 247 for female offspring, and 277 for offspring of both sexes (p
- Published
- 2018