1. Proteome analysis provides insights into sex differences in Holothuria Scabra.
- Author
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Cheng, Chuhang, Wu, FeiFei, Xu, Yizhi, Ren, Chunhua, Chen, Ting, Li, Shella, Shen, Peihong, and Jiang, Fajun
- Subjects
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PROTEOMICS , *POLYMERASE chain reaction , *SEA cucumbers , *PROTEIN expression , *GENETIC code , *GENETIC sex determination - Abstract
Sex-determining mechanism is still ambiguous for sea cucumber Holothuria scabra which only manifests gonochorism in gonad. In this study, proteomic analysis was employed to delineate sex-related proteins and genes in gonads of H. scabra, subsequently validated through Quantitative real-time polymerase chain reaction (qRT-PCR). A total of 5,313 proteins were identified via proteome sequencing. Among these, 817 proteins exhibited expression in both the ovary and testis, with 445 proteins displaying up-regulation and 372 proteins showing down-regulation (ovary vs testis). Furthermore, 136 and 69 proteins were identified as ovary-specific and testis-specific Differentially Abundant Proteins (DAPs), respectively. And 9 DAP coding genes which play crucial role in ovary and testis were verified by qRT-PCR. Notably, 24 ovary-bias proteins enriched in ribosome pathway strongly indicated the crucial role of ribosome in ovary. This study serves to furnish novel evidence pertaining to sex differences in H. scabra. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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