32 results on '"Qiu Yan"'
Search Results
2. Ginsenoside Rg3 inhibits melanoma cell proliferation through down-regulation of histone deacetylase 3 (HDAC3) and increase of p53 acetylation.
- Author
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Xiu Shan, Yuan-Shan Fu, Faisal Aziz, Xiao-Qi Wang, Qiu Yan, and Ji-Wei Liu
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Medicine ,Science - Abstract
Malignant melanoma is an aggressive and deadly form of skin cancer, and despite recent advances in available therapies, is still lacking in completely effective treatments. Rg3, a monomer extracted from ginseng roots, has been attempted for the treatment of many cancers. It is reported that the expressions of histone deacetylase 3 (HDAC3) and p53 acetylation correlate with tumor cell growth. However, the antitumor effect of Rg3 on melanoma and the mechanism by which it regulates HDAC3 expression and p53 acetylation remain unknown. We found high expression of HDAC3 in human melanoma tissues to be significantly correlated to lymph node metastasis and clinical stage of disease (p
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- 2014
- Full Text
- View/download PDF
3. Knockdown of EpCAM enhances the chemosensitivity of breast cancer cells to 5-fluorouracil by downregulating the antiapoptotic factor Bcl-2.
- Author
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Jiujiao Gao, Qiu Yan, Shuai Liu, and Xuesong Yang
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Medicine ,Science - Abstract
Resistance to fluoropyrimidine-based chemotherapy is the main reason for the failure of cancer treatment, and drug resistance is associated with an inability of tumor cells to undergo apoptosis in response to treatment. Alterations in the expression of epithelial cell adhesion molecule (EpCAM) affect the sensitivity or resistance of tumor cells to anticancer treatment and the activity of intracellular signaling pathways. However, the role of EpCAM in the induction of apoptosis in breast cancer cells remains unclear. Here, we investigated the effect of EpCAM gene knockdown on chemosensitivity to 5-fluorouracil (5-FU) in MCF-7 cells and explored the underlying mechanisms. Our results showed that knockdown of EpCAM promoted apoptosis, inhibited cell proliferation and caused cell-cycle arrest. EpCAM knockdown enhanced the cytotoxic effect of 5-FU, promoting apoptosis by downregulating the expression of the anti-apoptotic protein Bcl-2 and upregulating the expression of the pro-apoptotic proteins Bax, and caspase3 via the ERK1/2 and JNK MAPK signaling pathways in MCF-7 cells. These results indicate that knockdown of EpCAM may have a tumor suppressor effect and suggest EpCAM as a potential target for the treatment of breast cancer.
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- 2014
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- View/download PDF
4. Damage and failure of underground subway stations under different soil constraint conditions
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Zhang, Jing-Kun, primary, Yu, Zhong-Yang, additional, Zhang, Hong-Ru, additional, and Qiu, Yan-Jia, additional
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- 2023
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5. Integrated analysis of differential miRNA and mRNA expression profiles in human radioresistant and radiosensitive nasopharyngeal carcinoma cells
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Peng-Fei Zhang, Hong Liang Yi, Xu Ye, Jiao-Yang Li, Xin-Hui Li, Jia-Quan Qu, Qiu-Yan He, Zhi-Qiang Xiao, Jing-Feng Zhu, Li-li Yuan, Hong-Mei Yi, and Xun-Xun Wan
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Microarrays ,Radiation Biophysics ,Nasopharyngeal neoplasm ,Biophysics ,lcsh:Medicine ,Biology ,Radiation Tolerance ,RNA interference ,Molecular cell biology ,Radioresistance ,Cell Line, Tumor ,Gene expression ,microRNA ,Basic Cancer Research ,otorhinolaryngologic diseases ,medicine ,Genetics ,Humans ,RNA, Messenger ,RNA, Neoplasm ,lcsh:Science ,Gene ,Regulation of gene expression ,Multidisciplinary ,Nasopharyngeal Carcinoma ,Radiotherapy ,Carcinoma ,lcsh:R ,Computational Biology ,Radiobiology ,Cancers and Neoplasms ,Nasopharyngeal Neoplasms ,medicine.disease ,Molecular biology ,Head and Neck Tumors ,Gene Expression Regulation, Neoplastic ,stomatognathic diseases ,MicroRNAs ,Nasopharyngeal carcinoma ,Oncology ,Otorhinolaryngology ,Head and Neck Cancers ,Medicine ,lcsh:Q ,DNA microarray ,Research Article - Abstract
Background The purpose of this study was to identify miRNAs and genes involved in nasopharyngeal carcinoma (NPC) radioresistance, and explore the underlying mechanisms in the development of radioresistance. Methods We used microarrays to compare the differences of both miRNA and mRNA expression profiles in the radioresistant NPC CNE2-IR and radiosensitive NPC CNE2 cells, applied qRT-PCR to confirm the reliability of microarray data, adopted databases prediction and anticorrelated analysis of miRNA and mRNA expression to identify the miRNA target genes, and employed bioinformatics tools to examine the functions and pathways in which miRNA target genes are involved, and construct a miRNA-target gene regulatory network. We further investigated the roles of miRNA-23a and its target gene IL-8 in the NPC radioresistance. Results The main findings were fourfold: (1) fifteen differential miRNAs and 372 differential mRNAs were identified, and the reliability of microarray data was validated for randomly selected eight miRNAs and nine genes; (2) 174 miRNA target were identified, and most of their functions and regulating pathways were related to tumor therapeutic resistance; (3) a posttranscriptional regulatory network including 375 miRNA-target gene pairs was constructed, in which the ten genes were coregulated by the six miRNAs; (4) IL-8 was a direct target of miRNA-23a, the expression levels of IL-8 were elevated in the radioresistant NPC tissues and showed inverse correlation with miRNA-23a expression, and genetic upregulation of miRNA-23a and antibody neutralization of secretory IL-8 could reduce NPC cells radioresistance. Conclusions We identified fifteen differential miRNAs and 372 differential mRNAs in the radioresistant NPC cells, constructed a posttranscriptional regulatory network including 375 miRNA-target gene pairs, discovered the ten target genes coregulated by the six miRNAs, and validated that downregulated miRNA-23a was involved in NPC radioresistance through directly targeting IL-8. Our data form a basis for further investigating the mechanisms of NPC radioresistance.
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- 2014
6. Ginsenoside Rg3 inhibits melanoma cell proliferation through down-regulation of histone deacetylase 3 (HDAC3) and increase of p53 acetylation
- Author
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Jiwei Liu, Qiu Yan, Xiao Qi Wang, Faisal Aziz, Yuan Shan Fu, and Xiu Shan
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Male ,Small interfering RNA ,Ginsenosides ,Down-Regulation ,lcsh:Medicine ,Antineoplastic Agents ,Dermatology ,Biology ,Biochemistry ,Histone Deacetylases ,Small hairpin RNA ,Cell Line, Tumor ,medicine ,Medicine and Health Sciences ,Humans ,lcsh:Science ,Melanoma ,Aged ,Cell Proliferation ,Pharmacology ,Multidisciplinary ,Cell growth ,lcsh:R ,Biology and Life Sciences ,Acetylation ,Middle Aged ,medicine.disease ,HDAC3 ,Histone Deacetylase Inhibitors ,Oncology ,Cell culture ,Cancer research ,Female ,lcsh:Q ,Clinical Medicine ,Tumor Suppressor Protein p53 ,G1 phase ,Research Article - Abstract
Malignant melanoma is an aggressive and deadly form of skin cancer, and despite recent advances in available therapies, is still lacking in completely effective treatments. Rg3, a monomer extracted from ginseng roots, has been attempted for the treatment of many cancers. It is reported that the expressions of histone deacetylase 3 (HDAC3) and p53 acetylation correlate with tumor cell growth. However, the antitumor effect of Rg3 on melanoma and the mechanism by which it regulates HDAC3 expression and p53 acetylation remain unknown. We found high expression of HDAC3 in human melanoma tissues to be significantly correlated to lymph node metastasis and clinical stage of disease (p
- Published
- 2014
7. Knockdown of EpCAM enhances the chemosensitivity of breast cancer cells to 5-fluorouracil by downregulating the antiapoptotic factor Bcl-2
- Author
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Qiu Yan, Jiujiao Gao, Shuai Liu, and Xuesong Yang
- Subjects
MAP Kinase Signaling System ,Down-Regulation ,lcsh:Medicine ,Antineoplastic Agents ,Apoptosis ,Breast Neoplasms ,chemistry.chemical_compound ,Bcl-2-associated X protein ,Antigens, Neoplasm ,Cell Line, Tumor ,Molecular Cell Biology ,Gene Knockdown Techniques ,Cytotoxic T cell ,Humans ,lcsh:Science ,Molecular Biology ,Cell Proliferation ,bcl-2-Associated X Protein ,Gene knockdown ,Multidisciplinary ,biology ,Cell adhesion molecule ,Caspase 3 ,lcsh:R ,Biology and Life Sciences ,Epithelial cell adhesion molecule ,Cell Biology ,Cell Cycle Checkpoints ,Epithelial Cell Adhesion Molecule ,Molecular biology ,Gene Expression Regulation, Neoplastic ,Cell Motility ,chemistry ,Drug Resistance, Neoplasm ,Cancer research ,biology.protein ,MCF-7 Cells ,Female ,lcsh:Q ,Fluorouracil ,Signal transduction ,Cell Adhesion Molecules ,Research Article ,Signal Transduction - Abstract
Resistance to fluoropyrimidine-based chemotherapy is the main reason for the failure of cancer treatment, and drug resistance is associated with an inability of tumor cells to undergo apoptosis in response to treatment. Alterations in the expression of epithelial cell adhesion molecule (EpCAM) affect the sensitivity or resistance of tumor cells to anticancer treatment and the activity of intracellular signaling pathways. However, the role of EpCAM in the induction of apoptosis in breast cancer cells remains unclear. Here, we investigated the effect of EpCAM gene knockdown on chemosensitivity to 5-fluorouracil (5-FU) in MCF-7 cells and explored the underlying mechanisms. Our results showed that knockdown of EpCAM promoted apoptosis, inhibited cell proliferation and caused cell-cycle arrest. EpCAM knockdown enhanced the cytotoxic effect of 5-FU, promoting apoptosis by downregulating the expression of the anti-apoptotic protein Bcl-2 and upregulating the expression of the pro-apoptotic proteins Bax, and caspase3 via the ERK1/2 and JNK MAPK signaling pathways in MCF-7 cells. These results indicate that knockdown of EpCAM may have a tumor suppressor effect and suggest EpCAM as a potential target for the treatment of breast cancer.
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- 2014
8. Genetic variations in radiation and chemotherapy drug action pathways and survival in locoregionally advanced nasopharyngeal carcinoma treated with chemoradiotherapy
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Lu Zhang, Xing Lv, Bin Qi, Dong Hua Luo, Lin Quan Tang, Ming Huang Hong, Ming Yuan Chen, Xiang Guo, Yi Jun Hua, Ka Jia Cao, Qiu Yan Chen, Ling Guo, Ying Zhang, Lin Wang, Huai Liu, Hai Qiang Mai, Fang Qiu, Pei Yu Huang, Rui Sun, Chong Zhao, Chao Nan Qian, Yan Qun Xiang, and Hao Yuan Mo
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Oncology ,Adult ,Male ,Pathology ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,lcsh:Medicine ,Single-nucleotide polymorphism ,Disease-Free Survival ,Internal medicine ,medicine ,Humans ,lcsh:Science ,Aged ,Retrospective Studies ,Cisplatin ,Chemotherapy ,Multidisciplinary ,Nasopharyngeal Carcinoma ,Multifactor dimensionality reduction ,business.industry ,Carcinoma ,lcsh:R ,Genetic Variation ,Nasopharyngeal Neoplasms ,Chemoradiotherapy ,Middle Aged ,medicine.disease ,Neoplasm Proteins ,Radiation therapy ,Survival Rate ,Nasopharyngeal carcinoma ,Female ,lcsh:Q ,ERCC1 ,business ,medicine.drug ,Follow-Up Studies ,Research Article - Abstract
Background and Purpose: Treatment outcomes vary greatly in patients with nasopharyngeal carcinoma (NPC). The purpose of this study is to evaluate the influence of radiation and chemotherapy drug action pathway gene polymorphisms on the survival of patients with locoregionally advanced NPC treated with cisplatin- and fluorouracilbased chemoradiotherapy. Material and Methods: Four hundred twenty-one consecutive patients with locoregionally advanced NPC were prospectively recruited. We utilized a pathway approach and examined 18 polymorphisms in 13 major genes. Polymorphisms were detected using the LDR-PCR technique. Multifactor dimensionality reduction (MDR) analysis was performed to detect potential gene-gene interaction. Results: After adjustment for clinicopathological characteristics, overall survival was significantly decreased in patients with the MPO rs2243828 CT/CC genotype (HR=2.453, 95% CI, 1.687-3.566, P
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- 2013
9. Is Hemoglobin Level in Patients with Nasopharyngeal Carcinoma Still a Significant Prognostic Factor in the Era of Intensity-Modulated Radiotherapy Technology?
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Guo, Shan-Shan, primary, Tang, Lin-Quan, additional, Chen, Qiu-Yan, additional, Zhang, Lu, additional, Liu, Li-Ting, additional, Huang, Pei-Yu, additional, Cao, Ka-Jia, additional, Guo, Ling, additional, Mo, Hao-Yuan, additional, Guo, Xiang, additional, Hong, Ming-Huang, additional, Zeng, Mu-Sheng, additional, Qian, Chao-Nan, additional, and Mai, Hai-Qiang, additional
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- 2015
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10. Elevated High-Sensitivity C-Reactive Protein Levels Predict Decreased Survival for Nasopharyngeal Carcinoma Patients in the Intensity-Modulated Radiotherapy Era
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Tang, Lin Quan, primary, Hu, Dong Peng, additional, Chen, Qiu Yan, additional, Zhang, Lu, additional, Lai, Xiao Ping, additional, He, Yun, additional, Xu, Yun-Xiu-Xiu, additional, Wen, Shi-Hua, additional, Peng, Yu-Tuan, additional, Chen, Wen-Hui, additional, Guo, Shan-Shan, additional, Liu, Li-Ting, additional, Qian, Chao-Nan, additional, Guo, Xiang, additional, Zeng, Mu-Sheng, additional, and Mai, Hai-Qiang, additional
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- 2015
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11. Different Prognostic Values of Plasma Epstein-Barr Virus DNA and Maximal Standardized Uptake Value of 18F-FDG PET/CT for Nasopharyngeal Carcinoma Patients with Recurrence
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Shen, Ting, primary, Tang, Lin-Quan, additional, Luo, Dong-Hua, additional, Chen, Qiu-Yan, additional, Li, Pei-Jing, additional, Mai, Dong-Mei, additional, Guo, Shan-Shan, additional, Liu, Li-Ting, additional, Qian, Chao-Nan, additional, Guo, Xiang, additional, Zeng, Mu-Sheng, additional, Mo, Hao-Yuan, additional, and Mai, Hai-Qiang, additional
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- 2015
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12. Correlation between Activation of PI3K/AKT/mTOR Pathway and Prognosis of Breast Cancer in Chinese Women
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Deng, Ling, primary, Chen, Jie, additional, Zhong, Xiao Rong, additional, Luo, Ting, additional, Wang, Yan Ping, additional, Huang, Hui Fen, additional, Yin, Li-Juan, additional, Qiu, Yan, additional, Bu, Hong, additional, Lv, Qing, additional, and Zheng, Hong, additional
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- 2015
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13. Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy
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Li Zhang, Man-Zhi Li, Ulrika Rolén, Qiu-Yan Chen, Xiao-shi Zhang, Mu Sheng Zeng, Yan-fang Gao, Wenlin Huang, Yi Xin Zeng, Hao-Yuan Mo, Xiaoning Wang, Jiang Li, Maria G. Masucci, and Xue-hui Zeng
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Herpesvirus 4, Human ,T-Lymphocytes ,medicine.medical_treatment ,CD3 ,Science ,Immunology/Immunomodulation ,Nasopharyngeal neoplasm ,chemical and pharmacologic phenomena ,Virology/Immune Evasion ,Antibodies, Viral ,Lymphocyte Activation ,Immunophenotyping ,medicine ,otorhinolaryngologic diseases ,Humans ,Cytotoxic T cell ,IL-2 receptor ,In Situ Hybridization ,Multidisciplinary ,biology ,Tumor-infiltrating lymphocytes ,Chemistry ,Nasopharyngeal Neoplasms ,hemic and immune systems ,Immunotherapy ,Viral Load ,medicine.disease ,Virology/New Therapies, including Antivirals and Immunotherapy ,stomatognathic diseases ,Nasopharyngeal carcinoma ,Immunology/Immune Response ,Carrier State ,Immunology ,biology.protein ,Cytokines ,Medicine ,Research Article ,Virology/Viruses and Cancer - Abstract
Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV) associated malignancy with high prevalence in Southern Chinese. In order to assess whether defects of EBV-specific immunity may contribute to the tumor, the phenotype and function of circulating T-cells and tumor infiltrating lymphocytes (TILs) were investigated in untreated NPC patients. Circulating naïve CD3+CD45RA+ and CD4+CD25- cells were decreased, while activated CD4+CD25+ T-cells and CD3-CD16+ NK-cells were increased in patients compared to healthy donors. The frequency of T-cells recognizing seven HLA-A2 restricted epitopes in LMP1 and LMP2 was lower in the patients and remained low after stimulation with autologous EBV-carrying cells. TILs expanded in low doses of IL-2 exhibited an increase of CD3+CD4+, CD3+CD45RO+ and CD4+CD25+ cells and 2 to 5 fold higher frequency of LMP1 and LMP2 tetramer positive cells compared to peripheral blood. EBV-specific cytotoxicity could be reactivated from the blood of most patients, whereas the TILs lacked cytotoxic activity and failed to produce IFNgamma upon specific stimulation. Thus, EBV-specific rejection responses appear to be functionally inactivated at the tumor site in NPC.
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- 2007
14. Is Hemoglobin Level in Patients with Nasopharyngeal Carcinoma Still a Significant Prognostic Factor in the Era of Intensity-Modulated Radiotherapy Technology?
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Qiu-Yan Chen, Lu Zhang, Hai-Qiang Mai, Pei Yu Huang, Lin-Quan Tang, Xiang Guo, Ming-Huang Hong, Ka-Jia Cao, Mu Sheng Zeng, Ling Guo, Shan-Shan Guo, Chao-Nan Qian, Hao-Yuan Mo, and Li-Ting Liu
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,Anemia ,Nasopharyngeal neoplasm ,lcsh:Medicine ,Gastroenterology ,Hemoglobins ,Young Adult ,Internal medicine ,Carcinoma ,medicine ,Humans ,lcsh:Science ,Survival rate ,Aged ,Neoplasm Staging ,Retrospective Studies ,Nasopharyngeal Carcinoma ,Multidisciplinary ,business.industry ,Proportional hazards model ,lcsh:R ,Nasopharyngeal Neoplasms ,Middle Aged ,Prognosis ,medicine.disease ,Surgery ,Survival Rate ,Nasopharyngeal carcinoma ,lcsh:Q ,Female ,Radiotherapy, Intensity-Modulated ,Hemoglobin ,business ,Chemoradiotherapy ,Research Article - Abstract
Background Hemoglobin (Hb) levels are regarded as an important determinant of outcome in a number of cancers treated with radiotherapy. However, for patients treated with intensity modulated radiotherapy (IMRT), information regarding the prognostic value of hemoglobin level is scarce. Patients and Methods A total of 650 patients with nasopharyngeal carcinoma (NPC), enrolled between May, 2005, and November, 2012, were included in this study. The prognostic significance of hemoglobin level (anemia or no-anemia) at three different time points was investigated, including before treatment, during treatment and at the last week of treatment. Univariate and multivariate analyses were conducted using the log–rank test and the Cox proportional hazards model, respectively. Results The 5-year OS (overall survival) rate of patients who were anemia and no-anemia before treatment were 89.1%, and 80.7% (P = 0.01), respectively. The 5-year DMFS (distant metastasis-free survival) rate of patients who were anemia and no-anemia before treatment were 88.9%, and 78.2% (P = 0.01), respectively. The 5-year OS rate of patients who were anemia and no-anemia during treatment were 91.7% and 83.3% (P = 0.004). According to multivariate analysis, the pre-treatment Hb level predicted a decreased DMFS (P = 0.007, HR = 2.555, 95% CI1.294–5.046). Besides, the mid-treatment Hb level predicted a decreased OS (P = 0.013, HR = 2.333, 95% CI1.199–4.541). Conclusions Hemoglobin level is a useful prognostic factor in NPC patients receiving IMRT. It is important to control the level of hemoglobin both before and during chemoradiotherapy.
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- 2015
15. Elevated High-Sensitivity C-Reactive Protein Levels Predict Decreased Survival for Nasopharyngeal Carcinoma Patients in the Intensity-Modulated Radiotherapy Era
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Hai Qiang Mai, Xiao Ping Lai, Lin Quan Tang, Mu Sheng Zeng, Wen Hui Chen, Lu Zhang, Xiang Guo, Yun Xiu Xiu Xu, Yun He, Shi Hua Wen, Chao Nan Qian, Qiu Yan Chen, Yu Tuan Peng, Dong Peng Hu, Li Ting Liu, and Shan Shan Guo
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Adult ,Male ,medicine.medical_specialty ,Nasopharyngeal neoplasm ,lcsh:Medicine ,Kaplan-Meier Estimate ,Gastroenterology ,Disease-Free Survival ,Risk Factors ,Diabetes mellitus ,Internal medicine ,Blood plasma ,medicine ,Humans ,Progression-free survival ,lcsh:Science ,Survival rate ,Aged ,Neoplasm Staging ,Nasopharyngeal Carcinoma ,Multidisciplinary ,biology ,business.industry ,Carcinoma ,lcsh:R ,C-reactive protein ,Nasopharyngeal Neoplasms ,Middle Aged ,Prognosis ,medicine.disease ,C-Reactive Protein ,Nasopharyngeal carcinoma ,Immunology ,biology.protein ,Female ,lcsh:Q ,Radiotherapy, Intensity-Modulated ,Cisplatin ,business ,Body mass index ,Research Article - Abstract
Purpose This study aimed to clarify the prognostic utility of high-sensitivity C-reactive protein (hs-CRP) in nasopharyngeal carcinoma (NPC) patients in the Intensity-Modulated Radiotherapy (IMRT) era. Patients and Methods In this observational study, 1,589 non-metastatic NPC patients treated with IMRT were recruited. Blood samples were collected before treatment for examination of hs-CRP levels. We evaluated the association of pretreatment hs-CRP levels with overall survival rate (OS), progression free survival rate (PFS), locoregional relapse free survival rate (LRFS) and distant metastasis free survival rate (DMFS). Results Baseline hs-CRP levels were correlated with sex, clinical stage, body mass index, smoking status, and EBV DNA level. Multivariate analysis showed that hs-CRP had significant association with OS (HR:1.723; 95%CI:1.238–2.398; p = 0.001), PFS (HR:1.621; 95%CI:1.273–2.064; p
- Published
- 2015
16. Different Prognostic Values of Plasma Epstein-Barr Virus DNA and Maximal Standardized Uptake Value of 18F-FDG PET/CT for Nasopharyngeal Carcinoma Patients with Recurrence
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Lin Quan Tang, Mu Sheng Zeng, Hao Yuan Mo, Dong Hua Luo, Hai Qiang Mai, Chao Nan Qian, Shan Shan Guo, Pei Jing Li, Dong Mei Mai, Li Ting Liu, Ting Shen, Qiu Yan Chen, and Xiang Guo
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Adult ,Male ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,medicine.medical_specialty ,Pathology ,Adolescent ,medicine.medical_treatment ,Nasopharyngeal neoplasm ,lcsh:Medicine ,Standardized uptake value ,Gastroenterology ,Metastasis ,Fluorodeoxyglucose F18 ,Internal medicine ,Carcinoma ,medicine ,Humans ,lcsh:Science ,Child ,Survival analysis ,Aged ,Neoplasm Staging ,Nasopharyngeal Carcinoma ,Multidisciplinary ,business.industry ,lcsh:R ,Nasopharyngeal Neoplasms ,Middle Aged ,Prognosis ,medicine.disease ,Radiation therapy ,Nasopharyngeal carcinoma ,Positron-Emission Tomography ,DNA, Viral ,Biomarker (medicine) ,lcsh:Q ,Female ,Neoplasm Recurrence, Local ,Tomography, X-Ray Computed ,business ,Research Article - Abstract
Purpose To evaluate and compare the prognostic value of Epstein-Barr virus (EBV) DNA and maximal standard uptake values (SUVmax ) of 18F-fluoro-2-deoxy-D-glucose positron emission tomography (18F-FDG-PET) in subgroups of nasopharyngeal carcinoma (NPC) patients with locoregional or distant recurrence. Patients and Methods A total of 194 patients with recurrent NPC (locoregional recurrence: 107, distant recurrence: 87) were enrolled. Patients took evidence of recurrence performed with 18F-FDG-PET and an EBV DNA test before salvage treatment. Clinical parameters, the status of EBV DNA and the value of SUVmax were used for survival analysis using the Kaplan-Meier method and the Cox proportional hazards regression model. Results In the subgroup of patients with locoregional recurrence, patients with SUVmax
- Published
- 2015
17. Integrated Analysis of Differential miRNA and mRNA Expression Profiles in Human Radioresistant and Radiosensitive Nasopharyngeal Carcinoma Cells
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Li, Xin-Hui, primary, Qu, Jia-Quan, additional, Yi, Hong, additional, Zhang, Peng-Fei, additional, Yi, Hong-Mei, additional, Wan, Xun-Xun, additional, He, Qiu-Yan, additional, Ye, Xu, additional, Yuan, Li, additional, Zhu, Jing-Feng, additional, Li, Jiao-Yang, additional, and Xiao, Zhi-Qiang, additional
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- 2014
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18. Atheroprotective Effect of Oleoylethanolamide (OEA) Targeting Oxidized LDL
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Fan, Angran, primary, Wu, Xiaofeng, additional, Wu, Huijuan, additional, Li, Long, additional, Huang, Rui, additional, Zhu, Yueyong, additional, Qiu, Yan, additional, Fu, Jin, additional, Ren, Jie, additional, and Zhu, Chenggang, additional
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- 2014
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19. Genetic Variations in Radiation and Chemotherapy Drug Action Pathways and Survival in locoregionally Advanced Nasopharyngeal Carcinoma Treated with Chemoradiotherapy
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Liu, Huai, primary, Qi, Bin, additional, Guo, Xiang, additional, Tang, Lin-Quan, additional, Chen, Qiu-Yan, additional, Zhang, Lu, additional, Guo, Ling, additional, Luo, Dong-Hua, additional, Huang, Pei-Yu, additional, Mo, Hao-Yuan, additional, Xiang, Yan-Qun, additional, Qiu, Fang, additional, Sun, Rui, additional, Zhang, Ying, additional, Chen, Ming-Yuan, additional, Hua, Yi-Jun, additional, Lv, Xing, additional, Wang, Lin, additional, Zhao, Chong, additional, Cao, Ka-Jia, additional, Qian, Chao-Nan, additional, Hong, Ming-Huang, additional, and Mai, Hai-Qiang, additional
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- 2013
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20. Detection of VEGF-Axxxb Isoforms in Human Tissues
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Bates, David O., primary, Mavrou, Athina, additional, Qiu, Yan, additional, Carter, James G., additional, Hamdollah-Zadeh, Maryam, additional, Barratt, Shaney, additional, Gammons, Melissa V., additional, Millar, Ann B., additional, Salmon, Andrew H. J., additional, Oltean, Sebastian, additional, and Harper, Steven J., additional
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- 2013
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21. Transcriptome Analysis of Nicotiana tabacum Infected by Cucumber mosaic virus during Systemic Symptom Development
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Lu, Jie, primary, Du, Zhi-Xin, additional, Kong, Jun, additional, Chen, Ling-Na, additional, Qiu, Yan-Hong, additional, Li, Gui-Fen, additional, Meng, Xiao-Hua, additional, and Zhu, Shui-Fang, additional
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- 2012
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22. Design and Synthesis of Potent N-Acylethanolamine-hydrolyzing Acid Amidase (NAAA) Inhibitor as Anti-Inflammatory Compounds
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Li, Yuhang, primary, Yang, Longhe, additional, Chen, Ling, additional, Zhu, Chenggang, additional, Huang, Rui, additional, Zheng, Xiao, additional, Qiu, Yan, additional, and Fu, Jin, additional
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- 2012
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23. Non-microbial methane emissions from tropical rainforest soils under different conditions
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Gaohui Jia, Qiu Yang, Huai Yang, Yamin Jiang, Wenjie Liu, Tingting Wu, Han Mao, Tianyan Su, Zhenghong Tan, Xu Wang, and Juelei Li
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Medicine ,Science - Abstract
Non-microbial methane (NM-CH4), emissions from soil might play a significant role in carbon cycling and global climate change. However, the production mechanisms and emission potential of soil NM-CH4 from tropical rainforest remain highly uncertain. In order to explore the laws and characteristics of NM-CH4 emission from tropical rainforest soils. Incubation experiments at different environmental conditions (temperatures, soil water contents, hydrogen peroxide) and for soils with different soil organic carbon (SOC) contents were conducted to investigate the NM-CH4 emission characteristics and its influence factors of soils (0-10cm) that collected from a tropical rainforest in Hainan, China. Incubation results illustrated that soil NM-CH4 release showed a linear increase with the incubation time in the first 24 hours at 70 °C, whereas the logarithmic curve increase was found in 192 h incubation. Soil NM-CH4 emission rates under aerobic condition were significantly higher than that of under anaerobic condition at first 24 h incubation. The increasing of temperature, suitable soil water contents (0–100%), and hydrogen peroxide significantly promoted soil NM-CH4 emission rates at the first 24 h incubation. However, excessive soil water contents (200%) inhibited soil NM-CH4 emissions. According to the curve simulated from the NM-CH4 emission rates and incubation time at 70 °C of aerobic condition, soil would no longer release NM-CH4 after 229 h incubation. The NM-CH4 emissions were positively corelated with SOC contents, and the average soil NM-CH4 emission potential was about 6.91 ug per gram organic carbon in the tropical mountain rainforest. This study revealed that soils in the tropical rainforest could produce NM-CH4 under certain environment conditions and it supported production mechanisms of thermal degradation and reactive oxygen species oxidation. Those results could provide a basic data for understanding the soil NM-CH4 production mechanisms and its potential in the tropical rainforest.
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- 2021
24. Different Prognostic Values of Plasma Epstein-Barr Virus DNA and Maximal Standardized Uptake Value of 18F-FDG PET/CT for Nasopharyngeal Carcinoma Patients with Recurrence.
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Shen, Ting, Tang, Lin-Quan, Luo, Dong-Hua, Chen, Qiu-Yan, Li, Pei-Jing, Mai, Dong-Mei, Guo, Shan-Shan, Liu, Li-Ting, Qian, Chao-Nan, Guo, Xiang, Zeng, Mu-Sheng, Mo, Hao-Yuan, and Mai, Hai-Qiang
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NASOPHARYNX cancer patients ,EPSTEIN-Barr virus ,NUCLEOTIDE sequence ,BLOOD plasma ,POSITRON emission tomography ,CANCER relapse - Abstract
Purpose: To evaluate and compare the prognostic value of Epstein-Barr virus (EBV) DNA and maximal standard uptake values (SUV
max ) of18 F-fluoro-2-deoxy-D-glucose positron emission tomography (18 F-FDG-PET) in subgroups of nasopharyngeal carcinoma (NPC) patients with locoregional or distant recurrence. Patients and Methods: A total of 194 patients with recurrent NPC (locoregional recurrence: 107, distant recurrence: 87) were enrolled. Patients took evidence of recurrence performed with18 F-FDG-PET and an EBV DNA test before salvage treatment. Clinical parameters, the status of EBV DNA and the value of SUVmax were used for survival analysis using the Kaplan-Meier method and the Cox proportional hazards regression model. Results: In the subgroup of patients with locoregional recurrence, patients with SUVmax <8.65 had significantly better overall survival (OS) (P=0.005) compared with the patients with SUVmax ≥8.65. However, both elevated EBV DNA load (≥21,100 copies/ml) and distant SUVmax (≥13.55) were significantly associated with worse OS compared with the patients with EBV DNA <21,100 copies/ml or distant SUVmax <13.55 for the subgroup with distant recurrence (P=0.015 and P=0.006, respectively). The predictive ability of EBV DNA was superior to that of SUVmax (P=0.062). Multivariate analysis showed that SUVmax was only an independent prognostic factor for OS in patients with locoregional recurrence (P=0.042), whereas EBV DNA independently predicted OS for the patients with distant recurrence (P=0.007). For those patients with undetectable EBV DNA, SUVmax <8.65 was still an independent favorable prognostic factor (P=0.038). Conclusions: SUVmax is a useful biomarker for predicting OS in nasopharyngeal carcinoma patients with locoregional recurrence or with undetectable EBV DNA. Both distant SUVmax and EBV DNA appear to be independent predictors of OS in patients with distant recurrence; however, the predictive ability of EBV DNA was superior to that of SUVmax . [ABSTRACT FROM AUTHOR]- Published
- 2015
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25. Detection of VEGF-Axxxb Isoforms in Human Tissues.
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Bates, David O., Mavrou, Athina, Qiu, Yan, Carter, James G., Hamdollah-Zadeh, Maryam, Barratt, Shaney, Gammons, Melissa V., Millar, Ann B., Salmon, Andrew H. J., Oltean, Sebastian, and Harper, Steven J.
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VASCULAR endothelial growth factors ,ALTERNATIVE RNA splicing ,NEOVASCULARIZATION inhibitors ,GENE expression ,REVERSE transcriptase polymerase chain reaction ,IMMUNOGLOBULIN G ,EXPERIMENTAL design - Abstract
Vascular Endothelial Growth Factor-A (VEGF-A) can be generated as multiple isoforms by alternative splicing. Two families of isoforms have been described in humans, pro-angiogenic isoforms typified by VEGF-A
165 a, and anti-angiogenic isoforms typified by VEGF-A165 b. The practical determination of expression levels of alternative isoforms of the same gene may be complicated by experimental protocols that favour one isoform over another, and the use of specific positive and negative controls is essential for the interpretation of findings on expression of the isoforms. Here we address some of the difficulties in experimental design when investigating alternative splicing of VEGF isoforms, and discuss the use of appropriate control paradigms. We demonstrate why use of specific control experiments can prevent assumptions that VEGF-A165 b is not present, when in fact it is. We reiterate, and confirm previously published experimental design protocols that demonstrate the importance of using positive controls. These include using known target sequences to show that the experimental conditions are suitable for PCR amplification of VEGF-A165 b mRNA for both q-PCR and RT-PCR and to ensure that mispriming does not occur. We also provide evidence that demonstrates that detection of VEGF-A165 b protein in mice needs to be tightly controlled to prevent detection of mouse IgG by a secondary antibody. We also show that human VEGF165 b protein can be immunoprecipitated from cultured human cells and that immunoprecipitating VEGF-A results in protein that is detected by VEGF-A165 b antibody. These findings support the conclusion that more information on the biology of VEGF-A165 b isoforms is required, and confirm the importance of the experimental design in such investigations, including the use of specific positive and negative controls. [ABSTRACT FROM AUTHOR]- Published
- 2013
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26. Two Multiplex Real-Time PCR Assays to Detect and Differentiate Acinetobacter baumannii and Non- baumannii Acinetobacter spp. Carrying blaNDM, blaOXA-23-Like, blaOXA-40-Like, blaOXA-51-Like, and blaOXA-58-Like Genes.
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Qiu Yang and Yongyu Rui
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Medicine ,Science - Abstract
Nosocomial infections caused by Acinetobacter spp. resistant to carbapenems are increasingly reported worldwide. Carbapenem-resistant Acinetobacter (CRA) is becoming a serious concern with increasing patient morbidity, mortality, and lengths of hospital stay. Therefore, the rapid detection of CRA is essential for epidemiological surveillance. Polymerase chain reaction (PCR) has been extensively used for the rapid identification of most pathogens. In this study, we have developed two multiplex real-time PCR assays to detect and differentiate A. baumannii and non-A. baumannii Acinetobacter spp, and common carbapenemase genes, including blaNDM, blaOXA-23-like, blaOXA-40-like, blaOXA-51-like, and blaOXA-58-like. We demonstrate the potential utility of these assays for the direct detection of blaNDM-, blaOXA-23-like-, blaOXA-40-like-, blaOXA-51-like-, and blaOXA-58-like-positive CRA in clinical specimens. Primers were specifically designed, and two multiplex real-time PCR assays were developed: multiplex real-time PCR assay1 for the detection of Acinetobacter baumannii 16S-23S rRNA internal transcribed spacer sequence, the Acinetobacter recA gene, and class-B-metalloenzyme-encoding gene blaNDM; and multiplex real-time PCR assay2 to detect class-D-oxacillinase-encoding genes (blaOXA-23-like, blaOXA-40-like, blaOXA-51-like,and blaOXA-58-like). The assays were performed on an ABI Prism 7500 FAST Real-Time PCR System. CRA isolates were used to compare the assays with conventional PCR and sequencing. Known amounts of CRA cells were added to sputum and fecal specimens and used to test the multiplex real-time PCR assays. The results for target and nontarget amplification showed that the multiplex real-time PCR assays were specific, the limit of detection for each target was 10 copies per 20 μL reaction volume, the assays were linear over six log dilutions of the target genes (r2 > 0.99), and the Ct values of the coefficients of variation for intra- and interassay reproducibility were less than 5%. The multiplex real-time PCR assays showed 100% concordance with conventional PCR when tested against 400 CRA isolates and their sensitivity for the target DNA in sputum and fecal specimens was 102 CFU/mL. Therefore, these novel multiplex real-time PCR assays allow the sensitive and specific characterization and differentiation of blaNDM-, blaOXA-23-like-, blaOXA-40-like-, blaOXA-51-like-, and blaOXA-58-like-positive CRA, making them potential tools for the direct detection of CRA in clinical specimens and the surveillance of nosocomial infections.
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- 2016
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27. A meta-analysis of red yeast rice: an effective and relatively safe alternative approach for dyslipidemia.
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Yinhua Li, Long Jiang, Zhangrong Jia, Wei Xin, Shiwei Yang, Qiu Yang, and Luya Wang
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Medicine ,Science - Abstract
ObjectiveTo explore whether red yeast rice is a safe and effective alternative approach for dyslipidemia.MethodsPubmed, the Cochrane Library, EBSCO host, Chinese VIP Information (VIP), China National Knowledge Infrastructure (CNKI), Wanfang Databases were searched for appropriate articles. Randomized trials of RYR (not including Xuezhikang and Zhibituo) and placebo as control in patients with dyslipidemia were considered. Two authors read all papers and independently extracted all relevant information. The primary outcomes were serum total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG), and high-density lipoprotein cholesterol (HDL-C). The secondary outcomes were increased levels of alanine transaminase, aspartate aminotransferase, creatine kinase, creatinine and fasting blood glucose.ResultsA total of 13 randomized, placebo-controlled trials containing 804 participants were analyzed. Red yeast rice exhibited significant lowering effects on serum TC [WMD = -0.97 (95% CI: -1.13, -0.80) mmol/L, PConclusionsThe meta-analysis suggests that red yeast rice is an effective and relatively safe approach for dyslipidemia. However, further long-term, rigorously designed randomized controlled trials are still warranted before red yeast rice could be recommended to patients with dyslipidemia, especially as an alternative to statins.
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- 2014
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28. Different prognostic values of plasma Epstein-Barr virus DNA and maximal standardized uptake value of 18F-FDG PET/CT for nasopharyngeal carcinoma patients with recurrence.
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Ting Shen, Lin-Quan Tang, Dong-Hua Luo, Qiu-Yan Chen, Pei-Jing Li, Dong-Mei Mai, Shan-Shan Guo, Li-Ting Liu, Chao-Nan Qian, Xiang Guo, Mu-Sheng Zeng, Hao-Yuan Mo, and Hai-Qiang Mai
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Medicine ,Science - Abstract
PURPOSE:To evaluate and compare the prognostic value of Epstein-Barr virus (EBV) DNA and maximal standard uptake values (SUVmax ) of 18F-fluoro-2-deoxy-D-glucose positron emission tomography (18F-FDG-PET) in subgroups of nasopharyngeal carcinoma (NPC) patients with locoregional or distant recurrence. PATIENTS AND METHODS:A total of 194 patients with recurrent NPC (locoregional recurrence: 107, distant recurrence: 87) were enrolled. Patients took evidence of recurrence performed with 18F-FDG-PET and an EBV DNA test before salvage treatment. Clinical parameters, the status of EBV DNA and the value of SUVmax were used for survival analysis using the Kaplan-Meier method and the Cox proportional hazards regression model. RESULTS:In the subgroup of patients with locoregional recurrence, patients with SUVmax
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- 2015
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29. Is Hemoglobin Level in Patients with Nasopharyngeal Carcinoma Still a Significant Prognostic Factor in the Era of Intensity-Modulated Radiotherapy Technology?
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Shan-Shan Guo, Lin-Quan Tang, Qiu-Yan Chen, Lu Zhang, Li-Ting Liu, Pei-Yu Huang, Ka-Jia Cao, Ling Guo, Hao-Yuan Mo, Xiang Guo, Ming-Huang Hong, Mu-Sheng Zeng, Chao-Nan Qian, and Hai-Qiang Mai
- Subjects
Medicine ,Science - Abstract
BACKGROUND:Hemoglobin (Hb) levels are regarded as an important determinant of outcome in a number of cancers treated with radiotherapy. However, for patients treated with intensity modulated radiotherapy (IMRT), information regarding the prognostic value of hemoglobin level is scarce. PATIENTS AND METHODS:A total of 650 patients with nasopharyngeal carcinoma (NPC), enrolled between May, 2005, and November, 2012, were included in this study. The prognostic significance of hemoglobin level (anemia or no-anemia) at three different time points was investigated, including before treatment, during treatment and at the last week of treatment. Univariate and multivariate analyses were conducted using the log-rank test and the Cox proportional hazards model, respectively. RESULTS:The 5-year OS (overall survival) rate of patients who were anemia and no-anemia before treatment were 89.1%, and 80.7% (P = 0.01), respectively. The 5-year DMFS (distant metastasis-free survival) rate of patients who were anemia and no-anemia before treatment were 88.9%, and 78.2% (P = 0.01), respectively. The 5-year OS rate of patients who were anemia and no-anemia during treatment were 91.7% and 83.3% (P = 0.004). According to multivariate analysis, the pre-treatment Hb level predicted a decreased DMFS (P = 0.007, HR = 2.555, 95% CI1.294-5.046). Besides, the mid-treatment Hb level predicted a decreased OS (P = 0.013, HR = 2.333, 95% CI1.199-4.541). CONCLUSIONS:Hemoglobin level is a useful prognostic factor in NPC patients receiving IMRT. It is important to control the level of hemoglobin both before and during chemoradiotherapy.
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- 2015
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30. Integrated analysis of differential miRNA and mRNA expression profiles in human radioresistant and radiosensitive nasopharyngeal carcinoma cells.
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Xin-Hui Li, Jia-Quan Qu, Hong Yi, Peng-Fei Zhang, Hong-Mei Yi, Xun-Xun Wan, Qiu-Yan He, Xu Ye, Li Yuan, Jing-Feng Zhu, Jiao-Yang Li, and Zhi-Qiang Xiao
- Subjects
Medicine ,Science - Abstract
BACKGROUND:The purpose of this study was to identify miRNAs and genes involved in nasopharyngeal carcinoma (NPC) radioresistance, and explore the underlying mechanisms in the development of radioresistance. METHODS:We used microarrays to compare the differences of both miRNA and mRNA expression profiles in the radioresistant NPC CNE2-IR and radiosensitive NPC CNE2 cells, applied qRT-PCR to confirm the reliability of microarray data, adopted databases prediction and anticorrelated analysis of miRNA and mRNA expression to identify the miRNA target genes, and employed bioinformatics tools to examine the functions and pathways in which miRNA target genes are involved, and construct a miRNA-target gene regulatory network. We further investigated the roles of miRNA-23a and its target gene IL-8 in the NPC radioresistance. RESULTS:THE MAIN FINDINGS WERE FOURFOLD: (1) fifteen differential miRNAs and 372 differential mRNAs were identified, and the reliability of microarray data was validated for randomly selected eight miRNAs and nine genes; (2) 174 miRNA target were identified, and most of their functions and regulating pathways were related to tumor therapeutic resistance; (3) a posttranscriptional regulatory network including 375 miRNA-target gene pairs was constructed, in which the ten genes were coregulated by the six miRNAs; (4) IL-8 was a direct target of miRNA-23a, the expression levels of IL-8 were elevated in the radioresistant NPC tissues and showed inverse correlation with miRNA-23a expression, and genetic upregulation of miRNA-23a and antibody neutralization of secretory IL-8 could reduce NPC cells radioresistance. CONCLUSIONS:We identified fifteen differential miRNAs and 372 differential mRNAs in the radioresistant NPC cells, constructed a posttranscriptional regulatory network including 375 miRNA-target gene pairs, discovered the ten target genes coregulated by the six miRNAs, and validated that downregulated miRNA-23a was involved in NPC radioresistance through directly targeting IL-8. Our data form a basis for further investigating the mechanisms of NPC radioresistance.
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- 2014
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31. Genetic variations in radiation and chemotherapy drug action pathways and survival in locoregionally advanced nasopharyngeal carcinoma treated with chemoradiotherapy.
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Huai Liu, Bin Qi, Xiang Guo, Lin-Quan Tang, Qiu-Yan Chen, Lu Zhang, Ling Guo, Dong-Hua Luo, Pei-Yu Huang, Hao-Yuan Mo, Yan-Qun Xiang, Fang Qiu, Rui Sun, Ying Zhang, Ming-Yuan Chen, Yi-Jun Hua, Xing Lv, Lin Wang, Chong Zhao, Ka-Jia Cao, Chao-Nan Qian, Ming-Huang Hong, and Hai-Qiang Mai
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Medicine ,Science - Abstract
BACKGROUND AND PURPOSE:Treatment outcomes vary greatly in patients with nasopharyngeal carcinoma (NPC). The purpose of this study is to evaluate the influence of radiation and chemotherapy drug action pathway gene polymorphisms on the survival of patients with locoregionally advanced NPC treated with cisplatin- and fluorouracil-based chemoradiotherapy. MATERIAL AND METHODS:Four hundred twenty-one consecutive patients with locoregionally advanced NPC were prospectively recruited. We utilized a pathway approach and examined 18 polymorphisms in 13 major genes. Polymorphisms were detected using the LDR-PCR technique. Multifactor dimensionality reduction (MDR) analysis was performed to detect potential gene-gene interaction. RESULTS:After adjustment for clinicopathological characteristics, overall survival was significantly decreased in patients with the MPO rs2243828 CT/CC genotype (HR=2.453, 95% CI, 1.687-3.566, P
- Published
- 2013
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32. Functional inactivation of EBV-specific T-lymphocytes in nasopharyngeal carcinoma: implications for tumor immunotherapy.
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Jiang Li, Xue-hui Zeng, Hao-yuan Mo, Ulrika Rolén, Yan-fang Gao, Xiao-shi Zhang, Qiu-yan Chen, Li Zhang, Mu-sheng Zeng, Man-zhi Li, Wen-lin Huang, Xiao-ning Wang, Yi-xin Zeng, and Maria G Masucci
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Medicine ,Science - Abstract
Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV) associated malignancy with high prevalence in Southern Chinese. In order to assess whether defects of EBV-specific immunity may contribute to the tumor, the phenotype and function of circulating T-cells and tumor infiltrating lymphocytes (TILs) were investigated in untreated NPC patients. Circulating naïve CD3+CD45RA+ and CD4+CD25- cells were decreased, while activated CD4+CD25+ T-cells and CD3-CD16+ NK-cells were increased in patients compared to healthy donors. The frequency of T-cells recognizing seven HLA-A2 restricted epitopes in LMP1 and LMP2 was lower in the patients and remained low after stimulation with autologous EBV-carrying cells. TILs expanded in low doses of IL-2 exhibited an increase of CD3+CD4+, CD3+CD45RO+ and CD4+CD25+ cells and 2 to 5 fold higher frequency of LMP1 and LMP2 tetramer positive cells compared to peripheral blood. EBV-specific cytotoxicity could be reactivated from the blood of most patients, whereas the TILs lacked cytotoxic activity and failed to produce IFNgamma upon specific stimulation. Thus, EBV-specific rejection responses appear to be functionally inactivated at the tumor site in NPC.
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- 2007
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