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2 results on '"Monica Rezzonico"'

1. Quantitative detection of epstein-barr virus DNA in cerebrospinal fluid and blood samples of patients with relapsing-remitting multiple sclerosis.

Author

Clementina E Cocuzza, Fabrizio Piazza, Rosario Musumeci, Davide Oggioni, Simona Andreoni, Margherita Gardinetti, Letizia Fusco, Maura Frigo, Paola Banfi, Maria R Rottoli, Paolo Confalonieri, Monica Rezzonico, Maria T Ferrò, Guido Cavaletti, and EBV-MS Italian Study Group

Subjects
Medicine, Science
Abstract

The presence of Epstein-Barr Virus (EBV) DNA in cerebrospinal fluid (CSF) and peripheral blood (PB) samples collected from 55 patients with clinical and radiologically-active relapsing-remitting MS (RRMS) and 51 subjects with other neurological diseases was determined using standardized commercially available kits for viral nucleic acid extraction and quantitative EBV DNA detection. Both cell-free and cell-associated CSF and PB fractions were analyzed, to distinguish latent from lytic EBV infection. EBV DNA was detected in 5.5% and 18.2% of cell-free and cell-associated CSF fractions of patients with RRMS as compared to 7.8% and 7.8% of controls; plasma and peripheral blood mononuclear cells (PBMC) positivity rates were 7.3% and 47.3% versus 5.8% and 31.4%, respectively. No significant difference in median EBV viral loads of positive samples was found between RRMS and control patients in all tested samples. Absence of statistically significant differences in EBV positivity rates between RRMS and control patients, despite the use of highly sensitive standardized methods, points to the lack of association between EBV and MS disease activity.

Published
2014
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2. Natalizumab-Related Progressive Multifocal Leukoencephalopathy in Multiple Sclerosis: Findings from an Italian Independent Registry

Author

Prosperini, Luca, De Rossi, Nicola, Scarpazza, Cristina, Moiola, Lucia, Cosottini, Mirco, Gerevini, Simonetta, Capra, Ruggero, the Italian PML study group, Maria Pia Amato, Artusi, Carlo Alberto, Fabio, Bandini, Antonio, Bertolotto, Vincenzo, Bresciamorra, Guido, Cavaletti, Paolacavalla, Marco, Capobianco, Clerico, Marinella, Eleonora, Cocco, Giangaetano, D'Aleo, Marilena de Riz, Luciano, Deotto, Durelli, Luca, Mario, Falcini, Eugenio, Ferrari, Maria Luisa Fusco, Claudio, Gasperini, Simonetta, Gerevini, Angelo, Ghezzi, Luigi, Grimaldi, Mario, Guidotti, Alessandra, Lugaresi, Maria Giovanna Marrosu, Lucia, Moiola, Paola, Naldi, Patrizia, Perrone, Matteo, Pizzorno, Carlo, Pozzilli, Monica, Rezzonico, Marco, Rovaris, Giuseppe, Salemi, Marco, Salvetti, Giuseppe, Santuccio, Elio, Scarpini, Edoardo, Sessa, Claudio, Solaro, Giulia, Tabiadon, Carla, Tortorella, Maria, Trojano, and Paola, Valentino

Subjects
Male, Central Nervous System, Genetics and Molecular Biology (all), 0301 basic medicine, Pathology, Physiology, viruses, JC virus, lcsh:Medicine, Medicine (all), Biochemistry, Genetics and Molecular Biology (all), Agricultural and Biological Sciences (all), Pathology and Laboratory Medicine, medicine.disease_cause, Nervous System, Biochemistry, Diagnostic Radiology, Leukoencephalopathy, 0302 clinical medicine, Natalizumab, Medicine and Health Sciences, Ethnicities, lcsh:Science, Immune Response, Cerebrospinal Fluid, Multidisciplinary, Cognitive Neurology, Radiology and Imaging, Progressive multifocal leukoencephalopathy, Leukoencephalopathy, Progressive Multifocal, Middle Aged, JC Virus, Magnetic Resonance Imaging, Body Fluids, Italian People, Survival Rate, Neurology, Female, Anatomy, medicine.symptom, Research Article, medicine.drug, Adult, medicine.medical_specialty, Multiple Sclerosis, Imaging Techniques, Cognitive Neuroscience, Immunology, Progressive Multifocal, Research and Analysis Methods, Asymptomatic, Disease-Free Survival, Humans, Retrospective Studies, 03 medical and health sciences, Signs and Symptoms, Diagnostic Medicine, Internal medicine, medicine, Survival rate, Inflammation, Expanded Disability Status Scale, business.industry, Multiple sclerosis, lcsh:R, Biology and Life Sciences, medicine.disease, 030104 developmental biology, People and Places, Lesions, Cognitive Science, Population Groupings, lcsh:Q, business, 030217 neurology & neurosurgery, Neuroscience
Abstract

Background The monoclonal antibody natalizumab (NTZ) is a highly effective treatment for patients with multiple sclerosis (MS). However, this drug is associated with increased risk of developing Progressive Multifocal Leukoencephalopathy (PML), an opportunistic infection of central nervous system (CNS) caused by the John Cunningham polyomavirus (JCV). Objective To describe the 12-month clinical course of 39 patients with MS (28 women, 11 men) who developed NTZ-related PML after a mean exposure of 39 infusions. Methods An Italian independent collaborative repository initiative collected and analyzed socio-demographic, clinical, magnetic resonance imaging (MRI) data and number of JCV-DNA copies detected on cerebrospinal fluid (CSF) samples of patients diagnosed as affected by NTZ-related PML. The evolution of disability, measured by the Expanded Disability Status Scale, was assessed at NTZ start, at PML diagnosis and after 2, 6 and 12 months from PML diagnosis. The effect of clinical and paraclinical characteristics at PML diagnosis on the final outcome was also investigated. Results Ten patients (25.6%) were diagnosed before 24 NTZ infusions. In six cases (15.4%) the PML suspect was made on the basis of highly suggestive MRI findings in absence of any detectable change of clinical conditions (asymptomatic PML). In patients with symptomatic PML, the diagnosis was quicker for those who presented with cognitive symptoms (n = 12) rather than for those with other neurological pictures (n = 21) (p = 0.003). Three patients (7.7%) died during the 12-month observation period, resulting in a survival rate of 92.3%. Asymptomatic PML, more localized brain involvement and gadolinium-enhancement detected at MRI, as well as lower viral load were associated with a better disability outcome (p-values

Published
2016

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