130 results on '"Min, Jin"'
Search Results
2. Association between sleep patterns and alcohol use disorders in workers.
- Author
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Kim, Cho Rong, Kim, Soo Young, Kim, Jinhyun, Park, Eun-Cheol, and Ha, Min Jin
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ALCOHOLISM ,SLEEP ,SLEEP quality ,WORK environment ,MULTIPLE regression analysis - Abstract
Alcohol use among workers that is intended to aid sleep may lead to alcohol use disorders. This study aimed to explore the association between sleep patterns and alcohol use disorders in workers. Data from the Korea National Health and Nutrition Examination Survey conducted in 2014, 2016, 2018, and 2020 were used for this study. We included only workers aged 19 years and older. The final analysis comprised 11,972 respondents (6,472 male and 5,500 female). Multiple logistic regression analysis was used to investigate the relationship between sleep patterns and alcohol use disorders. Workers with poor sleep patterns were more likely to develop alcohol use disorders compared to those with good sleep patterns (male: adjusted odds ratio [OR] 1.22, 95% confidence interval 1.07–1.39; female: adjusted OR 1.21, 95% CI 1.03–1.41). Workers with both poor sleep quality and less than seven hours of sleep had the highest odds of alcohol use disorders in both male (adjusted OR 1.73, 95% CI 1.38–2.17) and female (adjusted OR 1.44, 95% CI 1.13–1.84). Poor sleep patterns were associated with alcohol use disorders in male who work night shift (OR: 1.74, 95% CI: 1.25–2.42) and in female who worked more than 52 hours per week (adjusted OR: 1.71, 95% CI: 1.04–2.80). Customized sleep management programs should be provided to workers in sleep-deprived working environments to prevent them from developing alcohol use disorders. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Risk factors for critical COVID-19 illness during Delta- and Omicron-predominant period in Korea; using K-COV-N cohort in the National health insurance service
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Lee, Kyung-Shin, primary, Go, Min Jin, additional, Choi, Youn Young, additional, Kim, Min-Kyung, additional, Seong, Jaehyun, additional, Sung, Ho Kyung, additional, Jeon, Jaehyun, additional, Jang, Hee-Chang, additional, and Kim, Myoung-Hee, additional
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- 2024
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4. Raldh1 promotes adiposity during adolescence independently of retinal signaling
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Yang, Di, Krois, Charles R, Huang, Priscilla, Wang, Jinshan, Min, Jin, Yoo, Hong Sik, Deng, Yinghua, and Napoli, Joseph L
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Biochemistry and Cell Biology ,Biomedical and Clinical Sciences ,Biological Sciences ,Nutrition ,Prevention ,Eye Disease and Disorders of Vision ,Obesity ,2.1 Biological and endogenous factors ,Aetiology ,Metabolic and endocrine ,Cardiovascular ,Adiposity ,Aldehyde Dehydrogenase 1 Family ,Animals ,Isoenzymes ,Mice ,Retinal Dehydrogenase ,Retinaldehyde ,Signal Transduction ,General Science & Technology - Abstract
All-trans-retinoic acid (RA) inhibits adipogenesis in established preadipocyte cell lines. Dosing pharmacological amounts of RA reduces weight gain in mice fed a high-fat diet, i.e. counteracts diet-induced obesity (DIO). The aldehyde dehydrogenase Raldh1 (Aldh1a1) functions as one of three enzymes that converts the retinol metabolite retinal into RA, and one of many proteins that contribute to RA homeostasis. Female Raldh1-ablated mice resist DIO. This phenotype contrasts with ablations of other enzymes and binding-proteins that maintain RA homeostasis, which gain adiposity. The phenotype observed prompted the conclusion that loss of Raldh1 causes an increase in adipose tissue retinal, and therefore, retinal functions independently of RA to prevent DIO. A second deduction proposed that low nM concentrations of RA stimulate adipogenesis, in contrast to higher concentrations. Using peer-reviewed LC/MS/MS assays developed and validated for quantifying tissue RA and retinal, we show that endogenous retinal and RA concentrations in adipose tissues from Raldh1-null mice do not correlate with the phenotype. Moreover, male Raldh1-null mice resist weight gain regardless of dietary fat content. Resistance to weight gain occurs during adolescence in both sexes. We show that RA concentrations as low as 1 nM, i.e. in the sub-physiological range, impair adipogenesis of embryonic fibroblasts from wild-type mice. Embryonic fibroblasts from Raldh1-null mice resist differentiating into adipocytes, but retain ability to generate RA. These fibroblasts remain sensitive to an RA receptor pan-agonist, and are not affected by an RA receptor pan-antagonist. Thus, the data do not support the hypothesis that retinal itself represses weight gain and adipogenesis independently of RA. Instead, the data indicate that Raldh1 functions as a retinal and atRA-independent promoter of adiposity during adolescence, and enhances adiposity through pre-adipocyte cell autonomous actions.
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- 2017
5. Low relative hand grip strength is associated with a higher risk for diabetes and impaired fasting glucose among the Korean population
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Min Jin Lee, Ah Reum Khang, Dongwon Yi, and Yang Ho Kang
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Medicine ,Science - Abstract
Objective This study investigated the association between relative hand grip strength (HGS) and glycemic status, such as impaired fasting glucose (IFG) and diabetes, using data from the Korea National Health and Nutrition Examination Survey (KNHANES). Methods We performed a cross-sectional study using the data from the KNHANES of 27,894 individuals from 2014 to 2019. Relative HGS was defined as the absolute HGS divided by body mass index and divided into quartiles in men and women. Odds ratios (OR) for diabetes and IFG were calculated using multivariate logistic regression analysis. All analyses were stratified by sex, and subgroup analysis was age-stratified. Results The lowest relative HGS quartile had a significant increase in the risk for diabetes (men: OR 2.72, 95% confidence interval [CI] 2.12–3.50; women: OR 3.38, 95% CI 2.70–4.24) and IFG (men: OR 1.35, 95% CI 1.15–1.59; women: OR 1.60, 95% CI 1.40–1.84). The ORs for diabetes and IFG according to the decreasing quartiles of relative HGS gradually increased in both sexes (P for trend Conclusion Lower relative HGS was associated with a higher risk of not only diabetes but also IFG in both sexes. These trends were stronger in younger adults than in older adults.
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- 2022
6. Association of a PAI-1 Gene Polymorphism and Early Life Infections with Asthma Risk, Exacerbations, and Reduced Lung Function.
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Cho, Seong H, Min, Jin-Young, Kim, Dong Young, Oh, Sam S, Torgerson, Dara R, Pino-Yanes, Maria, Hu, Donglei, Sen, Saunak, Huntsman, Scott, Eng, Celeste, Farber, Harold J, Rodriguez-Cintron, William, Rodriguez-Santana, Jose R, Serebrisky, Denise, Thyne, Shannon M, Borrell, Luisa N, Williams, L Keoki, DuPont, William, Seibold, Max A, Burchard, Esteban G, Avila, Pedro C, and Kumar, Rajesh
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Humans ,Respiratory Tract Infections ,Asthma ,Disease Progression ,Genetic Predisposition to Disease ,Plasminogen Activator Inhibitor 1 ,Respiratory Function Tests ,Odds Ratio ,Risk Assessment ,Risk Factors ,Case-Control Studies ,Genotype ,Polymorphism ,Genetic ,Alleles ,Socioeconomic Factors ,Adolescent ,Adult ,Child ,Ethnic Groups ,United States ,Female ,Male ,Young Adult ,Genetic Association Studies ,Polymorphism ,Genetic ,General Science & Technology - Abstract
BackgroundPlasminogen activator inhibitor-1 (PAI-1) is induced in airways by virus and may mediate asthmatic airway remodeling. We sought to evaluate if genetic variants and early life lower respiratory infections jointly affect asthma risk.MethodsWe included Latino children, adolescents, and young adults aged 8-21 years (1736 subjects with physician-diagnosed asthma and 1747 healthy controls) from five U.S. centers and Puerto Rico after excluding subjects with incomplete clinical or genetic data. We evaluated the independent and joint effects of a PAI-1 gain of function polymorphism and bronchiolitis / Respiratory Syncytial Virus (RSV) or other lower respiratory infections (LRI) within the first 2 years of life on asthma risk, asthma exacerbations and lung function.ResultsRSV infection (OR 9.9, 95%CI 4.9-20.2) and other LRI (OR 9.1, 95%CI 7.2-11.5) were independently associated with asthma, but PAI-1 genotype was not. There were joint effects on asthma risk for both genotype-RSV (OR 17.7, 95% CI 6.3-50.2) and genotype-LRI (OR 11.7, 95% CI 8.8-16.4). A joint effect of genotype-RSV resulted in a 3.1-fold increased risk for recurrent asthma hospitalizations. In genotype-respiratory infection joint effect analysis, FEV1% predicted and FEV1/FVC % predicted were further reduced in the genotype-LRI group (β -2.1, 95% CI -4.0 to -0.2; β -2.0, 95% CI -3.1 to -0.8 respectively). Similarly, lower FEV1% predicted was noted in genotype-RSV group (β -3.1, 95% CI -6.1 to -0.2) with a trend for lower FEV1/FVC % predicted.ConclusionsA genetic variant of PAI-1 together with early life LRI such as RSV bronchiolitis is associated with an increased risk of asthma, morbidity, and reduced lung function in this Latino population.
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- 2016
7. Improving the quality of sexual history disclosure on sex offenders: Emphasis on a polygraph examination
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Sue Hyun Jung, Min Jin Jin, Jang-Kyu Lee, Hee-Song Kim, Hyung-Ki Ji, Ki-Pyoung Kim, Myoung-Ho Hyun, Hyeon-Gi Hong, and Paolo Roma
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Medicine ,Science - Abstract
The increasing recidivism rate of sex offenders indicates potential problems in existing recidivism programs. The present study was conducted to determine whether the polygraph examination is a useful technique to obtain a sex offender’s concealed past sexual history. We collected fifty-two sex offenders’ data and analyzed it. Among the 52 participants, the court ordered 26 sex offenders to take the psychiatric evaluation and the polygraph test. The other half were prisoners at the hospital who were currently undergoing treatment. The participants in the polygraph group disclosed more deviant sexual behaviors and paraphilia interests/behaviors than the comparison group. Thus, the polygraph examination is a powerful tool that can encourage sex offenders to disclose hidden information to help create suitable psychological therapy programs for preventing recidivism in the future.
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- 2020
8. Association between pseudoexfoliation and Alzheimer’s disease-related brain atrophy
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Jeong, Won Cheol, primary, Min, Jin-Young, additional, Kang, Tae Gu, additional, and Bae, Heewon, additional
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- 2023
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9. Low relative hand grip strength is associated with a higher risk for diabetes and impaired fasting glucose among the Korean population
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Lee, Min Jin, primary, Khang, Ah Reum, additional, Yi, Dongwon, additional, and Kang, Yang Ho, additional
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- 2022
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10. Effect of behavioral inhibition system and childhood emotional neglect on serotonergic activity, negative affect, and rejection sensitivity in non-clinical adults.
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Min Jin Jin, Wookyoung Jung, Myoung Ho Hyun, and Seung-Hwan Lee
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Medicine ,Science - Abstract
INTRODUCTION:Behavioral inhibition system (BIS) has a strong genetic basis, and emotional neglect (EN) in childhood is one of many environmental experiences that can affect individuals. This study aimed to examine the effects and interaction between BIS and EN on central serotonergic activity and other negative affect and cognition. METHODS:A total of 153 non-clinical volunteers (54 men and 99 women; average age, 27.72 years, standard deviation = 6.40) were included in the analyses. The Behavioral Inhibition System scale, Childhood Trauma Questionnaire, and negative affect and cognition (Beck Depression Inventory, State-Trait Anxiety Inventory, and Rejection Sensitivity Questionnaire) were measured. As a biomarker of central serotonergic activity, the loudness dependence of auditory evoked potentials was measured. RESULTS:High EN was associated with higher loudness dependence of auditory evoked potential (LDAEP) levels and low EN was associated with lower LDAEP levels in high BIS people only. People with high EN people showed significantly higher levels of depression and state anxiety than did those with low EN. Moreover, of people with low BIS, those who had more EN experience had higher levels of rejection sensitivity than did those with less EN experience, while people with high BIS did not show different patterns of rejection sensitivity regardless of the difference of EN. CONCLUSIONS:This study revealed different effects on physiological (loudness dependence of auditory evoked potentials), intrapersonal (depression and state anxiety), and interpersonal aspects (rejection sensitivity) based on the interaction of BIS and EN. Our results suggest that the physiological and interpersonal aspects, but not the intrapersonal aspect, are significantly influenced by the interactive effect of BIS and EN.
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- 2018
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11. The impact of variations in input directions according to ISO 14243 on wearing of knee prostheses.
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Xiao-Hong Wang, Wei Zhang, Da-Yong Song, Hui Li, Xiang Dong, Min Zhang, Feng Zhao, Zhong-Min Jin, and Cheng-Kung Cheng
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Medicine ,Science - Abstract
ISO 14243 is the governing standard for wear testing of knee prostheses, but there is controversy over the correct direction of anterior-posterior (AP) displacement and loading and the correct direction of tibial rotation (TR) angles and torque. This study aimed to analyze how altering the direction of AP and TR affected wear on the tibial insert. Modifications to the conditions specified in ISO 14243-1 and ISO 14243-3 were also proposed. As such, five loading conditions were applied to FEA models of a knee prosthesis: (1) Modified ISO 14243-3 with positive AP displacement and TR angle, (2) ISO 14243-3:2004 with negative AP displacement and positive TR angle, (3) ISO 14243-3:2014 with positive AP displacement and negative TR angle, (4) Modified ISO 14243-1 with positive AP load and TR torque, and (5) ISO 14243-1:2009 with negative AP load and positive TR torque. This study found that changing the input directions for AP and TR according to ISO 14243-1 and 14243-3 had an influence on the wear rate and wear contours on the tibial insert model. However, the extent of wear varies depending on the design features of the tibial insert and shape of the input curves. For displacement control according to ISO 14243-3, changing the direction of AP displacement had a marked influence on the wear rate (272.77%), but changing the direction of TR angle had a much lower impact (2.17%). For load control according to ISO 14243-1, reversing the AP load (ISO 14243-1:2009) only increased the wear rate by 6.73% in comparison to the modified ISO 14243-1 conditions. The clinical relevance of this study is that the results demonstrate that tibial wear is affected by the direction of application of AP and TR. Incorrect application of the loading conditions during the design stage may lead to an ineffective preclinical evaluation and could subsequently influence implant longevity in clinical use.
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- 2018
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12. Transcriptome analysis of Macrobrachium rosenbergii intestines under the white spot syndrome virus and poly (I:C) challenges.
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Zhengfeng Ding, Min Jin, and Qian Ren
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Medicine ,Science - Abstract
Intestine is a primary site of the white spot syndrome virus (WSSV) infection in most crustaceans. To date, little is known about its role in the anti-viral immune response in the freshwater prawn Macrobrachium rosenbergii. In this study, next-generation sequencing was employed to investigate the M. rosenbergii intestine transcriptomes following WSSV or poly I:C challenges. A total of 41.06 M, 39.58 M and 47.00 M clean reads were generated and assembled into 65,340, 71,241 and 70,614 transcripts from the negative control group (NG), WSSV challenge group (WG) and poly I:C treatment group (PG) respectively. Based on homology searches, functional annotation with 7 databases (NR, NT, GO, COG, KEGG, Swissprot and Interpro) for 88,412 transcripts was performed. After WSSV or poly (I:C) challenge, the numbers of up-regulated differentially expressed genes (DEGs) were greater than the down-regulated DEGs. Gene Ontology (GO) classification of the DEGs also distributed similarly, with the same top 10 annotations and were all assigned to the signaling pathways, including spliceosome, Rap1 signaling pathway, proteoglycans, PI3K-Akt signaling pathway, ECM receptor interaction. Results could contribute to a better understanding of the intestinal immune response to viral pathogens.
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- 2018
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13. Dietary DHA/EPA ratio affected tissue fatty acid profiles, antioxidant capacity, hematological characteristics and expression of lipid-related genes but not growth in juvenile black seabream (Acanthopagrus schlegelii).
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Min Jin, Óscar Monroig, You Lu, Ye Yuan, Yi Li, Liyun Ding, Douglas R Tocher, and Qicun Zhou
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Medicine ,Science - Abstract
An 8-week feeding trial was conducted to investigate the effects of dietary docosahexaenoic to eicosapentaenoic acid ratio (DHA/EPA) on growth performance, fatty acid profiles, antioxidant capacity, hematological characteristics and expression of some lipid metabolism related genes of juvenile black seabream (Acanthopagrus schlegelii) of initial weight 9.47 ± 0.03 g. Five isonitrogenous and isolipidic diets (45% crude protein and 14% crude lipid) were formulated to contain graded DHA/EPA ratios of 0.65, 1.16, 1.60, 2.03 and 2.67. There were no differences in growth performance and feed utilization among treatments. Fish fed higher DHA/EPA ratios had higher malondialdehyde (MDA) contents in serum than lower ratios. Serum triacylglycerol (TAG) content was significantly higher in fish fed the lowest DHA/EPA ratio. Tissue fatty acid profiles reflected the diets despite down-regulation of LC-PUFA biosynthesis genes, fatty acyl desaturase 2 (fads2) and elongase of very long-chain fatty acids 5 (elovl5), by high DHA/EPA ratios. Expression of acetyl-CoA carboxylase alpha (accα) and carnitine palmitoyl transferase 1A (cpt1a) were up-regulated by high DHA/EPA ratio, whereas sterol regulatory element-binding protein-1 (srebp-1) and hormone-sensitive lipase (hsl) were down-regulated. Fatty acid synthase (fas), 6-phosphogluconate dehydrogenase (6pgd) and peroxisome proliferator-activated receptor alpha (pparα) showed highest expression in fish fed intermediate (1.16) DHA/EPA ratio. Overall, this study indicated that dietary DHA/EPA ratio affected fatty acid profiles and significantly influenced lipid metabolism including LC-PUFA biosynthesis and other anabolic and catabolic pathways, and also had impacts on antioxidant capacity and hematological characteristics.
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- 2017
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14. Dietary Lipid Sources Influence Fatty Acid Composition in Tissue of Large Yellow Croaker (Larmichthys crocea) by Regulating Triacylglycerol Synthesis and Catabolism at the Transcriptional Level.
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Hong Qiu, Min Jin, Yi Li, You Lu, Yingmei Hou, and Qicun Zhou
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Medicine ,Science - Abstract
An 8-week feeding trial was conducted to evaluate the effects of dietary lipid sources on growth performance, fatty acid composition, rate-limiting enzyme activities and gene expression related to lipid metabolism in large yellow croaker (Larmichthys crocea). Five iso-nitrogenous and iso-lipidic experimental diets were formulated to contain different lipid sources, such as fish oil (FO), soybean oil (SO), linseed oil (LO), rapeseed oil (RO) and peanut oil (PO), respectively. Triplicate groups of 50 fish (initial weight 13.77±0.07g) were stocked in 15 floating net cages (1.5m×1.5m×2.0m). Fish fed the diets containing RO and LO had lower weight gain and specific growth rates than those fed the FO, SO and PO diets. Survival, feed efficiency, protein efficiency ratio, hepatosomatic index, viscerasomatic index and condition factor were not significantly affected by different dietary lipid sources. Fish fed the diet containing FO had higher lipid content in whole body compared with the other groups, whereas fish fed the SO diet had the lowest muscle lipid content. Fatty acid profiles of muscle and liver reflected the fatty acid composition of the diets. Plasma glucose, triglyceride, and the enzymatic activity of aspartate aminotransferase and alanine aminotransferase were significantly influenced by different dietary lipid sources, while total protein, cholesterol, superoxide dismutase or malondialdehyde in plasma were not affected by the different dietary lipid sources. Fish fed the LO diet had lower adipose triglyceride lipase and fatty acid synthase activities in liver than those fed the diets containing FO and RO, while the LO diet resulted in the highest hepatic carnitine palmitoultransferase-1 activity. Hepatic gene relative expression of adipose triglyceride lipase and carnitine palmitoyltransferase-1 in fish fed PO diet was significantly higher than all other groups, whereas fish fed the SO and LO diets had lower relative expression levels of lipoprotein lipase than the other groups. The highest relative expression levels of fatty acid synthase and acyl-CoA diacylglycerol acyltransferase-2 were observed in the FO group, while the highest relative expression of glucose 6-phosphate dehydrogenase occurred in fish fed the FO and RO diets. In summary, based on the growth performance, FO and SO appear to be suitable lipid sources for large yellow croaker, with the findings of this study also providing a molecular insight into the role of lipid metabolic mechanism in response to different dietary lipid sources.
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- 2017
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15. Is it appropriate for Korean women to adopt the 2009 Institute of Medicine recommendations for gestational weight gain?
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Jeong Ha Wie, In Yang Park, Jeong Namkung, Hae Won Seo, Min Jin Jeong, and Ji Young Kwon
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Medicine ,Science - Abstract
The 2009 Institute of Medicine (IOM) guidelines for gestational weight gain (GWG) are intended for use among women in the United States. Little data are available on whether the 2009 IOM recommendations can be applied to Asian women. This study aimed to evaluate whether the recommendations are related to adverse pregnancy outcomes in Korean pregnant women.A retrospective cohort study was conducted for all singleton-pregnant women at a university hospital in Korea. After classifying the enrolled women into four Korean pre-pregnancy body mass index (BMI) categories, the risk of adverse pregnancy outcomes were analyzed for women who gained inadequate or excessive GWG based on 2009 IOM recommendations. Of 7,843 pregnancies, 64.0% of women had normal pre-pregnancy BMI and 42.7% achieved optimal GWG. Across all BMI categories, adverse pregnancies outcomes such as small for gestational age (SGA), large for gestational age (LGA), preterm birth, preeclampsia, and cesarean due to dystocia were significantly associated with GWG (all P ≤ 0.001).Women with normal BMI who gained inadequate weight were more likely to develop SGA and preterm birth and less likely to develop LGA (adjusted odds ratio (aOR) 2.21, 1.33, and 0.54, respectively). Whereas, women with normal BMI who gained excessive weight were more likely to develop LGA, preterm birth, preeclampsia, and cesarean section due to dystocia (aOR 2.10, 1.33, 1.37, and 1.37, respectively) and less likely to develop SGA (aOR 0.60).It is tolerable for Korean women to follow recommended GWG from the 2009 IOM guidelines to decrease adverse pregnancy outcomes. This will be helpful for antenatal care on GWG not only for Korean pregnant women, but also other Asian women who have lower BMI criteria than Caucasian women.
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- 2017
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16. Risk prediction of pulmonary tuberculosis using genetic and conventional risk factors in adult Korean population.
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Eun Pyo Hong, Min Jin Go, Hyung-Lae Kim, and Ji Wan Park
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Medicine ,Science - Abstract
A complex interplay among host, pathogen, and environmental factors is believed to contribute to the risk of developing pulmonary tuberculosis (PTB). The lack of replication of published genome-wide association study (GWAS) findings limits the clinical utility of reported single nucleotide polymorphisms (SNPs). We conducted a GWAS using 467 PTB cases and 1,313 healthy controls obtained from two community-based cohorts in Korea. We evaluated the performance of PTB risk models based on different combinations of genetic and nongenetic factors and validated the results in an independent Korean population comprised of 179 PTB cases and 500 healthy controls. We demonstrated the polygenic nature of PTB and nongenetic factors such as age, sex, and body mass index (BMI) were strongly associated with PTB risk. None of the SNPs achieved genome-wide significance; instead, we were able to replicate the associations between PTB and ten SNPs near or in the genes, CDCA7, GBE1, GADL1, SPATA16, C6orf118, KIAA1432, DMRT2, CTR9, CCDC67, and CDH13, which may play roles in the immune and inflammatory pathways. Among the replicated SNPs, an intergenic SNP, rs9365798, located downstream of the C6orf118 gene showed the most significant association under the dominant model (OR = 1.59, 95% CI 1.32-1.92, P = 2.1×10-6). The performance of a risk model combining the effects of ten replicated SNPs and six nongenetic factors (i.e., age, sex, BMI, cigarette smoking, systolic blood pressure, and hemoglobin) were validated in the replication set (AUC = 0.80, 95% CI 0.76-0.84). The strategy of combining genetic and nongenetic risk factors ultimately resulted in better risk prediction for PTB in the adult Korean population.
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- 2017
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17. Analysis of Sequence Variation and Risk Association of Human Papillomavirus 52 Variants Circulating in Korea.
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Youn Jin Choi, Eun Young Ki, Chuqing Zhang, Wendy C S Ho, Sung-Jong Lee, Min Jin Jeong, Paul K S Chan, and Jong Sup Park
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Medicine ,Science - Abstract
Human papillomavirus (HPV) 52 is a carcinogenic, high-risk genotype frequently detected in cervical cancer cases from East Asia, including Korea.Sequences of HPV52 detected in 91 cervical samples collected from women attending Seoul St. Mary's Hospital were analyzed. HPV52 genomic sequences were obtained by polymerase chain reaction (PCR)-based sequencing and analyzed using Seq-Scape software, and phylogenetic trees were constructed using MEGA6 software.Of the 91 cervical samples, 40 were normal, 22 were low-grade lesions, 21 were high-grade lesions and 7 were squamous cell carcinomas. Four HPV52 variant lineages (A, B, C and D) were identified. Lineage B was the most frequently detected lineage, followed by lineage C. By analyzing the two most frequently detected lineages (B and C), we found that distinct variations existed in each lineage. We also found that a lineage B-specific mutation K93R (A379G) was associated with an increased risk of cervical neoplasia.To our knowledge, we are the first to reveal the predominance of the HPV52 lineages, B and C, in Korea. We also found these lineages harbored distinct genetic alterations that may affect oncogenicity. Our findings increase our understanding on the heterogeneity of HPV52 variants, and may be useful for the development of new diagnostic assays and therapeutic vaccines.
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- 2016
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18. Plant Density Effect on Grain Number and Weight of Two Winter Wheat Cultivars at Different Spikelet and Grain Positions.
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Yong Li, Zhengyong Cui, Yingli Ni, Mengjing Zheng, Dongqing Yang, Min Jin, Jin Chen, Zhenlin Wang, and Yanping Yin
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Medicine ,Science - Abstract
In winter wheat, grain development is asynchronous. The grain number and grain weight vary significantly at different spikelet and grain positions among wheat cultivars grown at different plant densities. In this study, two winter wheat (Triticum aestivum L.) cultivars, 'Wennong6' and 'Jimai20', were grown under four different plant densities for two seasons, in order to study the effect of plant density on the grain number and grain weight at different spikelet and grain positions. The results showed that the effects of spikelet and grain positions on grain weight varied with the grain number of spikelets. In both cultivars, the single-grain weight of the basal and middle two-grain spikelets was higher at the 2nd grain position than that at the 1st grain position, while the opposite occurred in the top two-grain spikelets. In the three-grain spikelets, the distribution of the single-grain weight was different between cultivars. In the four-grain spikelets of Wennong6, the single-grain weight was the highest at the 2nd grain position, followed by the 1st, 3rd, and 4th grain positions. Regardless of the spikelet and grain positions, the single-grain weight was the highest at the 1st and 2nd grain positions and the lowest at the 3rd and 4th grain positions. Overall, plant density affected the yield by controlling the seed-setting characteristics of the tiller spike. Therefore, wheat yield can be increased by decreasing the sterile basal and top spikelets and enhancing the grain weight at the 3rd and 4th grain positions, while maintaining it at the 1st and 2nd grain positions on the spikelet.
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- 2016
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19. Comparative Analysis of Transcriptomes among Bombyx mori Strains and Sexes Reveals the Genes Regulating Melanic Morph and the Related Phenotypes.
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Songzhen He, Xiaoling Tong, Kunpeng Lu, Yaru Lu, Jiangwen Luo, Wenhao Yang, Min Chen, Min-Jin Han, Hai Hu, Cheng Lu, and Fangyin Dai
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Medicine ,Science - Abstract
As a source of insect polymorphism, melanism plays an important role in ecological adaption and usually endows advantageous phenotypic-effects on insects. However, due to the mechanistic diversity, there are knowledge gaps in the molecular mechanisms underlying melanism and the related phenotypes. In silk moths, a recessive melanic mutant (sex-controlled melanism, sml) strain exhibits extended adult longevity. We took a transcriptome approach to perform a comparative analysis between this sml strain and a wild-type strain (Dazao). Our analysis resulted in the identification of 59 unique differentially expressed genes in the melanic mutant. Two key genes (laccase2 and yellow) involved in melanin formation were significantly up-regulated in melanic individuals. The laccase2 B-type isoform (BGIBMGA006746) was found to likely participate in the silkworm cuticular melanism process at late pupal stage. Moreover, we discovered 22 cuticular protein encoding genes with the possible function in melanin transport and/or maintenance. Based on our findings, we presume that the longer survival of the melanic sml male moths might be associated with the enhanced antioxidant defense systems and a reduction in the insulin/IGF-1 signaling pathway (IIS). These findings will facilitate the understanding of the molecular basis underlying melanism and the derived phenotypic-effects.
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- 2016
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20. Two half-sandwiched ruthenium (II) compounds containing 5-fluorouracil derivatives: synthesis and study of DNA intercalation.
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Zhao-Jun Li, Yong Hou, Da-An Qin, Zhi-Min Jin, and Mao-Lin Hu
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Medicine ,Science - Abstract
Two novel coordination compounds of half-sandwiched ruthenium(II) containing 2-(5-fluorouracil)-yl-N-(pyridyl)-acetamide were synthesized, and their intercalation binding modes with calf thymus DNA were revealed by hyperchromism of ultraviolet-visible spectroscopy; the binding constants were determined according to a Langmuir adsorption equation that was deduced on the base of careful cyclic voltammetry measurements. The two compounds exhibited DNA intercalation binding activities with the binding constants of 1.13×106 M-1 and 5.35 ×105 M-1, respectively.
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- 2015
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21. A Novel Canine Model of Acute Vertebral Artery Occlusion.
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Yunfeng Zhang, Min Jin, Bin Du, Hao Lin, Chengyong Xu, Weijian Jiang, and Jianping Jia
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Medicine ,Science - Abstract
The extended time window and theoretic reduction in hemorrhage make mechanical strategies an attractive approach for the treatment of patients with ischemic stroke. However, a limited availability of suitable animal models of cerebrovascular thrombosis has hampered the study of novel endovascular interventions. The aim of the present study was to develop a new technique for site-specific placement of a thrombus in a canine model that would allow for the evaluation of mechanical thrombectomy and clot retrieval methods and the visualization of thrombus dislocation or fragmentation during angiographic manipulation.Angiography and embolization with a preformed thrombus were performed in 12 canines. Under fluoroscopic guidance, an embolism protection device (EPD) was anchored to the middle segment of the left vertebral artery (VA) via the left femoral arterial sheath. A preformed radiopaque clot was injected through the guide catheter into the left VA, via the contralateral femoral artery, proximal to the EPD. After 15 min of occlusion, the EPD was removed and persistent occlusion of the VA was documented angiographically.Angiography performed during the observation period confirmed the persistence of VA occlusion in each case, and displacement of the radiopaque clots did not occur during the 3-hour observation period. The technique allowed selective embolization of targeted vessels without thrombus fragmentation.This study demonstrates, for the first time, a canine model of post-circulation embolism induced by autologous blood clot placement. This model can be rapidly formed and easily operated, and the site of thrombosis can be readily controlled.
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- 2015
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22. Correction: Risk factors for critical COVID-19 illness during Delta- and Omicron-predominant period in Korea; using K-COV-N cohort in the National health insurance service.
- Author
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Lee, Kyung-Shin, Go, Min Jin, Choi, Youn Young, Kim, Min-Kyung, Seong, Jaehyun, Sung, Ho Kyung, Jeon, Jaehyun, Jang, Hee-Chang, and Kim, Myoung-Hee
- Subjects
- *
NATIONAL health insurance , *COVID-19 pandemic , *CRITICALLY ill - Abstract
This document is a correction notice for a study titled "Risk factors for critical COVID-19 illness during Delta- and Omicron-predominant period in Korea; using K-COV-N cohort in the National health insurance service." The correction pertains to the funding statement, which was published incorrectly. The correct funding statement acknowledges that the study was conducted as part of a public-private joint research effort on COVID-19, co-hosted by the KDCA (Korea Disease Control and Prevention Agency) and the NHIS (National Health Insurance Service) in South Korea. The study also received support from the Korea National Institute of Health. The funders did not have any involvement in the study design, data collection and analysis, decision to publish, or manuscript preparation. [Extracted from the article]
- Published
- 2024
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23. Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia
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Min-Woo Lee, Ho Jae Lim, Min Jin Kim, Sun Hwa Lee, Kyoung Ho Roh, Min Young Park, Yong-Jin Yang, Jae-Hyun Yang, Bo Kyung Kim, Eun-Rim Kang, Nackmoon Sung, and Sunkyung Jung
- Subjects
0301 basic medicine ,Microbiological culture ,Pulmonology ,Physiology ,Staphylococcus ,Antibiotics ,Artificial Gene Amplification and Extension ,Pathology and Laboratory Medicine ,Polymerase Chain Reaction ,Klebsiella Pneumoniae ,Moraxella catarrhalis ,0302 clinical medicine ,Klebsiella ,Medicine and Health Sciences ,Multiplex ,030212 general & internal medicine ,Multidisciplinary ,Respiratory tract infections ,biology ,Pseudomonas Aeruginosa ,Pneumococcus ,Antimicrobial ,Bacterial Pathogens ,Body Fluids ,Medical Microbiology ,Medicine ,medicine.symptom ,Pathogens ,Anatomy ,Pneumonia (non-human) ,Research Article ,Staphylococcus aureus ,medicine.drug_class ,Science ,030106 microbiology ,Research and Analysis Methods ,Microbiology ,Sensitivity and Specificity ,03 medical and health sciences ,Pseudomonas ,medicine ,Humans ,Molecular Biology Techniques ,Microbial Pathogens ,Molecular Biology ,Bacteria ,business.industry ,Organisms ,Sputum ,Biology and Life Sciences ,Streptococcus ,Pneumonia ,biology.organism_classification ,medicine.disease ,Mucus ,business ,Multiplex Polymerase Chain Reaction - Abstract
Classification of clinical symptoms and diagnostic microbiology are essential to effectively employ antimicrobial therapy for lower respiratory tract infections (LRTIs) in a timely manner. Empirical antibiotic treatment without microbial identification hinders the selective use of narrow-spectrum antibiotics and effective patient treatment. Thus, the development of rapid and accurate diagnostic procedures that can be readily adopted by the clinic is necessary to minimize non-essential or excessive use of antibiotics and accelerate patient recovery from LRTI-induced damage. We developed and validated a multiplex real-time polymerase chain reaction (mRT-PCR) assay with good analytical performance and high specificity to simultaneously detect four bacterial pathogens causing pneumonia: Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Moraxella catarrhalis. The analytical performance of mRT-PCR against target pathogens was evaluated by the limit of detection (LOD), specificity, and repeatability. Two hundred and ten clinical specimens from pneumonia patients were processed using an automatic nucleic acid extraction system for the “respiratory bacteria four” (RB4) mRT-PCR assay, and the results were directly compared to references from bacterial culture and/or Sanger sequencing. The RB4 mRT-PCR assay detected all target pathogens from sputum specimens with a coefficient of variation ranging from 0.29 to 1.71 and conservative LOD of DNA corresponding to 5 × 102 copies/reaction. The concordance of the assay with reference-positive specimens was 100%, and additional bacterial infections were detected from reference-negative specimens. Overall, the RB4 mRT-PCR assay showed a more rapid turnaround time and higher performance that those of reference assays. The RB4 mRT-PCR assay is a high-throughput and reliable tool that assists decision-making assessment and outperforms other standard methods. This tool supports patient management by considerably reducing the inappropriate use of antibiotics.
- Published
- 2021
24. Correction: Improving the quality of sexual history disclosure on sex offenders: Emphasis on a polygraph examination
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Jung, Sue Hyun, primary, Jin, Min Jin, additional, Lee, Jang-Kyu, additional, Kim, Hee-Song, additional, Ji, Hyung-Ki, additional, Kim, Ki-Pyoung, additional, Hyun, Myoung-Ho, additional, and Hong, Hyeon-Gi, additional
- Published
- 2021
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25. Correction: The Transcriptomic Signature of RacA Activation and Inactivation Provides New Insights into the Morphogenetic Network of.
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Min Jin Kwon, Benjamin M. Nitsche, Mark Arentshorst, Thomas R. Jørgensen, Arthur F. J. Ram, and Vera Meyer
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Medicine ,Science - Published
- 2014
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26. Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia
- Author
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Lim, Ho Jae, primary, Kang, Eun-Rim, additional, Park, Min Young, additional, Kim, Bo Kyung, additional, Kim, Min Jin, additional, Jung, Sunkyung, additional, Roh, Kyoung Ho, additional, Sung, Nackmoon, additional, Yang, Jae-Hyun, additional, Lee, Min-Woo, additional, Lee, Sun-Hwa, additional, and Yang, Yong-Jin, additional
- Published
- 2021
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27. A knowledge-based multivariate statistical method for examining gene-brain-behavioral/cognitive relationships: Imaging genetics generalized structured component analysis
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Seung Hwan Lee, Gyeongcheol Cho, Heungsun Hwang, Min Jin Jin, Ji Hoon Ryoo, and Younyoung Choi
- Subjects
Genotype ,Imaging genetics ,Imaging Techniques ,Science ,Single Nucleotide Polymorphisms ,Social Sciences ,Single-nucleotide polymorphism ,Neuroimaging ,Affect (psychology) ,Research and Analysis Methods ,Polymorphism, Single Nucleotide ,Cognition ,Component analysis ,Genetics ,Humans ,Psychology ,Genetic Predisposition to Disease ,Serotonin transporter ,Behavior ,Evolutionary Biology ,Multidisciplinary ,biology ,Population Biology ,Brain ,Biology and Life Sciences ,Models, Theoretical ,Phenotypes ,Phenotype ,Multicollinearity ,Multivariate Analysis ,Genetics of Disease ,biology.protein ,Genetic Polymorphism ,Medicine ,Gene-Environment Interaction ,Algorithms ,Population Genetics ,Cognitive psychology ,Research Article ,Neuroscience - Abstract
With advances in neuroimaging and genetics, imaging genetics is a naturally emerging field that combines genetic and neuroimaging data with behavioral or cognitive outcomes to examine genetic influence on altered brain functions associated with behavioral or cognitive variation. We propose a statistical approach, termed imaging genetics generalized structured component analysis (IG-GSCA), which allows researchers to investigate such gene-brain-behavior/cognitive associations, taking into account well-documented biological characteristics (e.g., genetic pathways, gene-environment interactions, etc.) and methodological complexities (e.g., multicollinearity) in imaging genetic studies. We begin by describing the conceptual and technical underpinnings of IG-GSCA. We then apply the approach for investigating how nine depression-related genes and their interactions with an environmental variable (experience of potentially traumatic events) influence the thickness variations of 53 brain regions, which in turn affect depression severity in a sample of Korean participants. Our analysis shows that a dopamine receptor gene and an interaction between a serotonin transporter gene and the environment variable have statistically significant effects on a few brain regions’ variations that have statistically significant negative impacts on depression severity. These relationships are largely supported by previous studies. We also conduct a simulation study to safeguard whether IG-GSCA can recover parameters as expected in a similar situation.
- Published
- 2020
28. A knowledge-based multivariate statistical method for examining gene-brain-behavioral/cognitive relationships: Imaging genetics generalized structured component analysis
- Author
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Hwang, Heungsun, primary, Cho, Gyeongcheol, additional, Jin, Min Jin, additional, Ryoo, Ji Hoon, additional, Choi, Younyoung, additional, and Lee, Seung Hwan, additional
- Published
- 2021
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29. A rapid subtractive immunization method to prepare discriminatory monoclonal antibodies for food E. coli O157:H7 contamination.
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Min Jin, Jing Lang, Zhi-Qiang Shen, Zhao-Li Chen, Zhi-Gang Qiu, Xin-Wei Wang, and Jun-Wen Li
- Subjects
Medicine ,Science - Abstract
To detect food E. coli O157:H7 contamination rapidly and accurately, it is essential to prepare high specific monoclonal antibodies (mAbs) against the pathogen. Cyclophosphamide (Cy)-mediated subtractive immunization strategy was performed in mice to generate mAbs that react with E. coli O157:H7, but not with other affiliated bacteria. Specificity of 19 mAbs was evaluated by ELISA and/or dot-immunogold filtration assay (DIGFA). Immunogloubin typing, affinity and binding antigens of 5 selected mAbs were also analysed. MAbs 1D8, 4A7, 5A2 were found to have high reactivity with E. coli O157:H7 and no cross-reactivity with 80 other strains of bacteria including Salmonella sp., Shigella sp., Proteus sp., Yersinia enterocolitica, Staphylococcus aureus, Klebsiella pneumoniae, Citrobacter freundii and other non-E. coli O157:H7 enteric bacteria. Their ascetic titers reached 1:10(6) with E. coli O157:H7 and affinity constants ranged from 1.57 × 10(10) to 2.79 × 10(10) L/mol. The antigens recognized by them were different localized proteins. Furthermore, immune-colloidal gold probe coated with mAb 5A2 could specifically distinguish minced beef contaminated by E. coli O157:H7 from 84 other bacterial contaminations. The Cy-mediated subtractive immunization procedure coupled with hybridoma technology is a rapid and efficient approach to prepare discriminatory mAbs for detection of E. coli O157:H7 contamination in food.
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- 2012
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30. Alteration of CXCR7 expression mediated by TLR4 promotes tumor cell proliferation and migration in human colorectal carcinoma.
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Huanbai Xu, Qiong Wu, Shipeng Dang, Min Jin, Jingwei Xu, Yiji Cheng, Minglin Pan, Yugang Wu, Chunhui Zhang, and Yanyun Zhang
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Medicine ,Science - Abstract
The link between inflammation and colorectal carcinoma has been acknowledged. However, the impact of bacterial lipopolysaccharide (LPS) binding to Toll-like receptor 4 (TLR4) on chemokine receptors in human colorectal carcinoma cells still remains to be elucidated. The present study shows that exposure to LPS elevated CXC chemokine receptor 7 (CXCR7) expression in colorectal carcinoma SW480 and Colo 205 cell lines expressing TLR4/myeloid differential protein (MD-2). CXCR7 is associated with SW480 cell proliferation and migration. However, exposure of SW480 and Colo 205 cells to LPS had no effect on CXCR4 expression. To further support the above results, the expression of TLR4, MD-2, and CXCR7 was analyzed in human colorectal carcinoma tissues. Higher rates of TLR4 (53%), MD-2 (70%), and CXCR7 (29%) expression were found in colorectal carcinoma tissues than in normal tissues. We demonstrated that the recombination of TLR4, MD-2 and CXCR7 strongly correlated with tumor size, lymph node metastasis and distant metastasis in colorectal carcinoma tissue samples (p = 0.037, p = 0.002, p = 0.042, resp.). Accordingly, simultaneous examination of the expression of TLR4, MD-2 and CXCR7 in cancer tissues of colorectal carcinoma may provide valuable prognostic diagnosis of carcinoma growth and metastasis. Interplay of TLR4, MD-2 and CXCR7 may be of interest in the context of novel immunomodulatory therapies for colorectal carcinoma.
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- 2011
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31. Purinergic activation of Ca2+-permeable TRPV4 channels is essential for mechano-sensitivity in the aldosterone-sensitive distal nephron.
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Mykola Mamenko, Oleg Zaika, Min Jin, Roger G O'Neil, and Oleh Pochynyuk
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Medicine ,Science - Abstract
Mechanical forces are known to induce increases of [Ca(2+)](i) in the aldosterone-sensitive distal nephron (ASDN) cells to regulate epithelial transport. At the same time, mechanical stress stimulates ATP release from ASDN cells. In this study, we combined ratiometric Fura-2 based monitoring of [Ca(2+)](i) in freshly isolated split-opened ASDN with targeted deletion of P2Y2 and TRPV4 in mice to probe a role for purinergic signaling in mediating mechano-sensitive responses in ASDN cells. ATP application causes a reproducible transient Ca(2+) peak followed by a sustained plateau. Individual cells of the cortical collecting duct (CCD) and the connecting tubule (CNT) respond to purinergic stimulation with comparative elevations of [Ca(2+)](i). Furthermore, ATP-induced Ca(2+)-responses are nearly identical in both principal (AQP2-positive) and intercalated (AQP2-negative) cells as was confirmed using immunohistochemistry in split-opened ASDN. UTP application produces elevations of [Ca(2+)](i) similar to that observed with ATP suggesting a dominant role of P2Y2-like receptors in generation of [Ca(2+)](i) response. Indeed, genetic deletion of P2Y2 receptors decreases the magnitude of ATP-induced and UTP-induced Ca(2+) responses by more than 70% and 90%, respectively. Both intracellular and extracellular sources of Ca(2+) appeared to contribute to the generation of ATP-induced Ca(2+) response in ASDN cells. Importantly, flow- and hypotonic-induced Ca(2+) elevations are markedly blunted in P2Y2 -/- mice. We further demonstrated that activation of mechano-sensitive TRPV4 channel plays a major role in the sustained [Ca(2+)](i) elevation during purinergic stimulation. Consistent with this, ATP-induced Ca(2+) plateau are dramatically attenuated in TRV4 -/- mice. Inhibition of TRPC channels with 10 µM BTP2 also decreased ATP-induced Ca(2+) plateau whilst to a lower degree than that observed with TRPV4 inhibition/genetic deletion. We conclude that stimulation of purinergic signaling by mechanical stimuli leads to activation of TRPV4 and, to a lesser extent, TRPCs channels, and this is an important component of mechano-sensitive response of the ASDN.
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- 2011
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32. Correction: Improving the quality of sexual history disclosure on sex offenders: Emphasis on a polygraph examination
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Hyeon Gi Hong, Sue Hyun Jung, Myoung Ho Hyun, Ki Pyoung Kim, Jang Kyu Lee, Hee Song Kim, Min Jin Jin, and Hyung Ki Ji
- Subjects
Male ,Questionnaires ,Epidemiology ,Psychologists ,Lie Detection ,Social Sciences ,Criminology ,Rape and Sexual Assault ,Sociology ,Medicine and Health Sciences ,Psychology ,Public and Occupational Health ,Medical Personnel ,media_common ,Multidisciplinary ,Paraphilic Disorders ,Sex offender ,Traumatic Injury Risk Factors ,Clinical Laboratory Sciences ,Test (assessment) ,Psychological evaluation ,Clinical Psychology ,Professions ,Research Design ,Medicine ,Paraphilia ,Sexual history ,Crime ,Clinical psychology ,Research Article ,Adult ,media_common.quotation_subject ,Sexual Behavior ,Science ,Truth Disclosure ,Research and Analysis Methods ,Polygraph ,Diagnostic Medicine ,Mental Health and Psychiatry ,medicine ,Humans ,Quality (business) ,Violent Crime ,Forensics ,Behavior ,Survey Research ,Recidivism ,Prisoners ,Sex Offenses ,Cognitive Psychology ,Correction ,Biology and Life Sciences ,Criminals ,medicine.disease ,Medical Risk Factors ,People and Places ,Cognitive Science ,Population Groupings ,Mental Health Therapies ,Neuroscience - Abstract
The increasing recidivism rate of sex offenders indicates potential problems in existing recidivism programs. The present study was conducted to determine whether the polygraph examination is a useful technique to obtain a sex offender's concealed past sexual history. We collected fifty-two sex offenders' data and analyzed it. Among the 52 participants, the court ordered 26 sex offenders to take the psychiatric evaluation and the polygraph test. The other half were prisoners at the hospital who were currently undergoing treatment. The participants in the polygraph group disclosed more deviant sexual behaviors and paraphilia interests/behaviors than the comparison group. Thus, the polygraph examination is a powerful tool that can encourage sex offenders to disclose hidden information to help create suitable psychological therapy programs for preventing recidivism in the future.
- Published
- 2021
33. Improving the quality of sexual history disclosure on sex offenders: Emphasis on a polygraph examination
- Author
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Jung, Sue Hyun, primary, Jin, Min Jin, additional, Lee, Jang-Kyu, additional, Kim, Hee-Song, additional, Ji, Hyung-Ki, additional, Kim, Ki-Pyoung, additional, Hyun, Myoung-Ho, additional, and Hong, Hyeon-Gi, additional
- Published
- 2020
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34. Effect of behavioral inhibition system and childhood emotional neglect on serotonergic activity, negative affect, and rejection sensitivity in non-clinical adults
- Author
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Jin, Min Jin, primary, Jung, Wookyoung, additional, Hyun, Myoung Ho, additional, and Lee, Seung-Hwan, additional
- Published
- 2018
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35. Dietary Lipid Sources Influence Fatty Acid Composition in Tissue of Large Yellow Croaker (Larmichthys crocea) by Regulating Triacylglycerol Synthesis and Catabolism at the Transcriptional Level
- Author
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Qicun Zhou, Yi Li, Hong Qiu, Min Jin, Lu You, and Yingmei Hou
- Subjects
0301 basic medicine ,Marine and Aquatic Sciences ,Gene Expression ,lcsh:Medicine ,Biochemistry ,chemistry.chemical_compound ,Linseed oil ,Plant Products ,Medicine and Health Sciences ,Marine Fish ,Food science ,lcsh:Science ,chemistry.chemical_classification ,Multidisciplinary ,Muscles ,Fatty Acids ,Fishes ,Agriculture ,04 agricultural and veterinary sciences ,Fish oil ,Lipids ,Fatty acid synthase ,Liver ,Vertebrates ,Peanut Oil ,Research Article ,Fish Proteins ,medicine.medical_specialty ,Linseed Oil ,food.ingredient ,Dietary lipid ,Marine Biology ,Glucosephosphate Dehydrogenase ,Biology ,Vegetable Oils ,03 medical and health sciences ,Fish Oils ,food ,Internal medicine ,Genetics ,medicine ,Animals ,Plant Oils ,Diacylglycerol O-Acyltransferase ,Triglycerides ,Nutrition ,Triglyceride ,lcsh:R ,Organisms ,Biology and Life Sciences ,Fatty acid ,Lipid metabolism ,Lipase ,Lipid Metabolism ,Dietary Fats ,Agronomy ,Perciformes ,Soybean Oil ,Diet ,Metabolism ,030104 developmental biology ,Endocrinology ,chemistry ,Adipose triglyceride lipase ,Earth Sciences ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,lcsh:Q ,Fatty Acid Synthases ,Oils ,Crop Science - Abstract
An 8-week feeding trial was conducted to evaluate the effects of dietary lipid sources on growth performance, fatty acid composition, rate-limiting enzyme activities and gene expression related to lipid metabolism in large yellow croaker (Larmichthys crocea). Five iso-nitrogenous and iso-lipidic experimental diets were formulated to contain different lipid sources, such as fish oil (FO), soybean oil (SO), linseed oil (LO), rapeseed oil (RO) and peanut oil (PO), respectively. Triplicate groups of 50 fish (initial weight 13.77±0.07g) were stocked in 15 floating net cages (1.5m×1.5m×2.0m). Fish fed the diets containing RO and LO had lower weight gain and specific growth rates than those fed the FO, SO and PO diets. Survival, feed efficiency, protein efficiency ratio, hepatosomatic index, viscerasomatic index and condition factor were not significantly affected by different dietary lipid sources. Fish fed the diet containing FO had higher lipid content in whole body compared with the other groups, whereas fish fed the SO diet had the lowest muscle lipid content. Fatty acid profiles of muscle and liver reflected the fatty acid composition of the diets. Plasma glucose, triglyceride, and the enzymatic activity of aspartate aminotransferase and alanine aminotransferase were significantly influenced by different dietary lipid sources, while total protein, cholesterol, superoxide dismutase or malondialdehyde in plasma were not affected by the different dietary lipid sources. Fish fed the LO diet had lower adipose triglyceride lipase and fatty acid synthase activities in liver than those fed the diets containing FO and RO, while the LO diet resulted in the highest hepatic carnitine palmitoultransferase-1 activity. Hepatic gene relative expression of adipose triglyceride lipase and carnitine palmitoyltransferase-1 in fish fed PO diet was significantly higher than all other groups, whereas fish fed the SO and LO diets had lower relative expression levels of lipoprotein lipase than the other groups. The highest relative expression levels of fatty acid synthase and acyl-CoA diacylglycerol acyltransferase-2 were observed in the FO group, while the highest relative expression of glucose 6-phosphate dehydrogenase occurred in fish fed the FO and RO diets. In summary, based on the growth performance, FO and SO appear to be suitable lipid sources for large yellow croaker, with the findings of this study also providing a molecular insight into the role of lipid metabolic mechanism in response to different dietary lipid sources.
- Published
- 2017
36. Relationship among family environment, self-control, friendship quality, and adolescents’ smartphone addiction in South Korea: Findings from nationwide data
- Author
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Kim, Hye-Jin, primary, Min, Jin-Young, additional, Min, Kyoung-Bok, additional, Lee, Tae-Jin, additional, and Yoo, Seunghyun, additional
- Published
- 2018
- Full Text
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37. Analysis of Sequence Variation and Risk Association of Human Papillomavirus 52 Variants Circulating in Korea
- Author
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Chuqing Zhang, Eun Young Ki, Youn Jin Choi, Sung Jong Lee, Paul K.S. Chan, Min Jin Jeong, Jong Sup Park, and Wendy C. S. Ho
- Subjects
0301 basic medicine ,Gene Identification and Analysis ,Uterine Cervical Neoplasms ,lcsh:Medicine ,Artificial Gene Amplification and Extension ,Pathology and Laboratory Medicine ,Cervical Cancer ,Polymerase Chain Reaction ,law.invention ,0302 clinical medicine ,law ,Genotype ,Medicine and Health Sciences ,Papillomaviridae ,lcsh:Science ,Polymerase chain reaction ,Cervical cancer ,Genetics ,Multidisciplinary ,biology ,Phylogenetic tree ,Squamous Cell Carcinomas ,Phylogenetic Analysis ,Oncology ,Medical Microbiology ,Viral Pathogens ,030220 oncology & carcinogenesis ,Viruses ,Carcinoma, Squamous Cell ,Female ,Pathogens ,Sequence Analysis ,Research Article ,Lineage (genetic) ,Papillomaviruses ,Sequence analysis ,Nucleotide Sequencing ,Research and Analysis Methods ,Microbiology ,Carcinomas ,03 medical and health sciences ,Republic of Korea ,Genetic variation ,medicine ,Humans ,Molecular Biology Techniques ,Sequencing Techniques ,Molecular Biology ,Microbial Pathogens ,Mutation Detection ,Molecular Biology Assays and Analysis Techniques ,Papillomavirus Infections ,lcsh:R ,Organisms ,Genetic Variation ,Biology and Life Sciences ,Human Papillomavirus ,Cancers and Neoplasms ,Sequence Analysis, DNA ,biology.organism_classification ,medicine.disease ,030104 developmental biology ,DNA, Viral ,lcsh:Q ,DNA viruses ,Gynecological Tumors - Abstract
Introduction Human papillomavirus (HPV) 52 is a carcinogenic, high-risk genotype frequently detected in cervical cancer cases from East Asia, including Korea. Materials and methods Sequences of HPV52 detected in 91 cervical samples collected from women attending Seoul St. Mary's Hospital were analyzed. HPV52 genomic sequences were obtained by polymerase chain reaction (PCR)-based sequencing and analyzed using Seq-Scape software, and phylogenetic trees were constructed using MEGA6 software. Results Of the 91 cervical samples, 40 were normal, 22 were low-grade lesions, 21 were high-grade lesions and 7 were squamous cell carcinomas. Four HPV52 variant lineages (A, B, C and D) were identified. Lineage B was the most frequently detected lineage, followed by lineage C. By analyzing the two most frequently detected lineages (B and C), we found that distinct variations existed in each lineage. We also found that a lineage B-specific mutation K93R (A379G) was associated with an increased risk of cervical neoplasia. Conclusions To our knowledge, we are the first to reveal the predominance of the HPV52 lineages, B and C, in Korea. We also found these lineages harbored distinct genetic alterations that may affect oncogenicity. Our findings increase our understanding on the heterogeneity of HPV52 variants, and may be useful for the development of new diagnostic assays and therapeutic vaccines.
- Published
- 2016
38. Comparative Analysis of Transcriptomes among Bombyx mori Strains and Sexes Reveals the Genes Regulating Melanic Morph and the Related Phenotypes
- Author
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Ya-Ru Lu, Fangyin Dai, Jiangwen Luo, Cheng Lu, Min Chen, Songzhen He, Wenhao Yang, Kunpeng Lu, Xiaoling Tong, Min-Jin Han, and Hai Hu
- Subjects
0301 basic medicine ,Pigments ,Male ,Gene Expression ,lcsh:Medicine ,Insect ,Transcriptome ,Melanin ,lcsh:Science ,media_common ,Genetics ,Multidisciplinary ,biology ,Longevity ,Genomics ,Phenotype ,Insects ,Phenotypes ,Moths and Butterflies ,Physical Sciences ,Female ,Transcriptome Analysis ,Research Article ,Arthropoda ,media_common.quotation_subject ,Materials Science ,03 medical and health sciences ,Bombyx mori ,Botany ,Animals ,Gene ,Melanism ,Materials by Attribute ,Bombyx ,Organic Pigments ,fungi ,lcsh:R ,Organisms ,Biology and Life Sciences ,Computational Biology ,biology.organism_classification ,Genome Analysis ,Invertebrates ,030104 developmental biology ,Silkworms ,lcsh:Q - Abstract
As a source of insect polymorphism, melanism plays an important role in ecological adaption and usually endows advantageous phenotypic-effects on insects. However, due to the mechanistic diversity, there are knowledge gaps in the molecular mechanisms underlying melanism and the related phenotypes. In silk moths, a recessive melanic mutant (sex-controlled melanism, sml) strain exhibits extended adult longevity. We took a transcriptome approach to perform a comparative analysis between this sml strain and a wild-type strain (Dazao). Our analysis resulted in the identification of 59 unique differentially expressed genes in the melanic mutant. Two key genes (laccase2 and yellow) involved in melanin formation were significantly up-regulated in melanic individuals. The laccase2 B-type isoform (BGIBMGA006746) was found to likely participate in the silkworm cuticular melanism process at late pupal stage. Moreover, we discovered 22 cuticular protein encoding genes with the possible function in melanin transport and/or maintenance. Based on our findings, we presume that the longer survival of the melanic sml male moths might be associated with the enhanced antioxidant defense systems and a reduction in the insulin/IGF-1 signaling pathway (IIS). These findings will facilitate the understanding of the molecular basis underlying melanism and the derived phenotypic-effects.
- Published
- 2016
39. Transcriptome analysis of Macrobrachium rosenbergii intestines under the white spot syndrome virus and poly (I:C) challenges
- Author
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Min Jin, Qian Ren, and Zhengfeng Ding
- Subjects
0301 basic medicine ,White spot syndrome ,Glycobiology ,Gene Expression ,lcsh:Medicine ,Biochemistry ,Homology (biology) ,Transcriptome ,Nucleic Acids ,Gene expression ,Medicine and Health Sciences ,lcsh:Science ,Immune Response ,Genetics ,Multidisciplinary ,biology ,Gene Ontologies ,Eukaryota ,Genomics ,04 agricultural and veterinary sciences ,DNA Virus Infections ,Crustaceans ,Intestines ,Proteoglycans ,Palaemonidae ,Anatomy ,Transcriptome Analysis ,Research Article ,Arthropoda ,Immunology ,Polymorphism, Single Nucleotide ,Virus ,03 medical and health sciences ,White spot syndrome virus 1 ,Animals ,KEGG ,Gene ,Macrobrachium rosenbergii ,lcsh:R ,Organisms ,Biology and Life Sciences ,Computational Biology ,Genome Analysis ,biology.organism_classification ,Invertebrates ,Immunity, Innate ,Gastrointestinal Tract ,Poly I-C ,030104 developmental biology ,Spliceosomes ,040102 fisheries ,RNA ,0401 agriculture, forestry, and fisheries ,lcsh:Q ,Digestive System ,Microsatellite Repeats - Abstract
Intestine is a primary site of the white spot syndrome virus (WSSV) infection in most crustaceans. To date, little is known about its role in the anti-viral immune response in the freshwater prawn Macrobrachium rosenbergii. In this study, next-generation sequencing was employed to investigate the M. rosenbergii intestine transcriptomes following WSSV or poly I:C challenges. A total of 41.06 M, 39.58 M and 47.00 M clean reads were generated and assembled into 65,340, 71,241 and 70,614 transcripts from the negative control group (NG), WSSV challenge group (WG) and poly I:C treatment group (PG) respectively. Based on homology searches, functional annotation with 7 databases (NR, NT, GO, COG, KEGG, Swissprot and Interpro) for 88,412 transcripts was performed. After WSSV or poly (I:C) challenge, the numbers of up-regulated differentially expressed genes (DEGs) were greater than the down-regulated DEGs. Gene Ontology (GO) classification of the DEGs also distributed similarly, with the same top 10 annotations and were all assigned to the signaling pathways, including spliceosome, Rap1 signaling pathway, proteoglycans, PI3K-Akt signaling pathway, ECM receptor interaction. Results could contribute to a better understanding of the intestinal immune response to viral pathogens.
- Published
- 2018
40. Is it appropriate for Korean women to adopt the 2009 Institute of Medicine recommendations for gestational weight gain?
- Author
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Wie, Jeong ha, primary, Park, In Yang, additional, Namkung, Jeong, additional, Seo, Hae Won, additional, Jeong, Min Jin, additional, and Kwon, Ji Young, additional
- Published
- 2017
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41. Risk prediction of pulmonary tuberculosis using genetic and conventional risk factors in adult Korean population
- Author
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Hong, Eun Pyo, primary, Go, Min Jin, additional, Kim, Hyung-Lae, additional, and Park, Ji Wan, additional
- Published
- 2017
- Full Text
- View/download PDF
42. Analysis of Sequence Variation and Risk Association of Human Papillomavirus 52 Variants Circulating in Korea
- Author
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Choi, Youn Jin, primary, Ki, Eun Young, additional, Zhang, Chuqing, additional, Ho, Wendy C. S., additional, Lee, Sung-Jong, additional, Jeong, Min Jin, additional, Chan, Paul K. S., additional, and Park, Jong Sup, additional
- Published
- 2016
- Full Text
- View/download PDF
43. Two half-sandwiched ruthenium (II) compounds containing 5-fluorouracil derivatives: synthesis and study of DNA intercalation
- Author
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Yong Hou, Mao-Lin Hu, Da-An Qin, Zhi-Min Jin, and Zhao-Jun Li
- Subjects
Intercalation (chemistry) ,lcsh:Medicine ,chemistry.chemical_element ,Ruthenium ,Coordination complex ,chemistry.chemical_compound ,Ultraviolet visible spectroscopy ,Coordination Complexes ,Animals ,lcsh:Science ,Voltammetry ,Electrodes ,chemistry.chemical_classification ,Multidisciplinary ,lcsh:R ,DNA ,Electrochemical Techniques ,Intercalating Agents ,Crystallography ,DNA Intercalation ,chemistry ,lcsh:Q ,Cattle ,Spectrophotometry, Ultraviolet ,Fluorouracil ,Cyclic voltammetry ,Algorithms ,Research Article - Abstract
Two novel coordination compounds of half-sandwiched ruthenium(II) containing 2-(5-fluorouracil)-yl-N-(pyridyl)-acetamide were synthesized, and their intercalation binding modes with calf thymus DNA were revealed by hyperchromism of ultraviolet-visible spectroscopy; the binding constants were determined according to a Langmuir adsorption equation that was deduced on the base of careful cyclic voltammetry measurements. The two compounds exhibited DNA intercalation binding activities with the binding constants of 1.13×106 M-1 and 5.35 ×105 M-1, respectively.
- Published
- 2014
44. Dietary DHA/EPA ratio affected tissue fatty acid profiles, antioxidant capacity, hematological characteristics and expression of lipid-related genes but not growth in juvenile black seabream (Acanthopagrus schlegelii)
- Author
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Óscar Monroig, Qicun Zhou, Douglas R. Tocher, Liyun Ding, Yi Li, Ye Yuan, Lu You, and Min Jin
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0301 basic medicine ,Gene Expression ,Marine and Aquatic Sciences ,lcsh:Medicine ,Biochemistry ,Antioxidants ,Medicine and Health Sciences ,Marine Fish ,Enzyme Chemistry ,lcsh:Science ,chemistry.chemical_classification ,Multidisciplinary ,biology ,Fatty Acids ,Fishes ,food and beverages ,Black seabream ,04 agricultural and veterinary sciences ,Lipids ,Eicosapentaenoic acid ,Fatty acid synthase ,Eicosapentaenoic Acid ,Docosahexaenoic acid ,Vertebrates ,lipids (amino acids, peptides, and proteins) ,Research Article ,medicine.drug ,medicine.medical_specialty ,Docosahexaenoic Acids ,FADS2 ,Marine Biology ,Biosynthesis ,Enzyme Regulation ,03 medical and health sciences ,Internal medicine ,Genetics ,medicine ,Animals ,Carnitine ,Nutrition ,lcsh:R ,Organisms ,Biology and Life Sciences ,Fatty acid ,Lipid metabolism ,biology.organism_classification ,Sea Bream ,Diet ,030104 developmental biology ,Endocrinology ,chemistry ,Earth Sciences ,Enzymology ,040102 fisheries ,biology.protein ,0401 agriculture, forestry, and fisheries ,lcsh:Q - Abstract
An 8-week feeding trial was conducted to investigate the effects of dietary docosahexaenoic to eicosapentaenoic acid ratio (DHA/EPA) on growth performance, fatty acid profiles, antioxidant capacity, hematological characteristics and expression of some lipid metabolism related genes of juvenile black seabream (Acanthopagrus schlegelii) of initial weight 9.47 ± 0.03 g. Five isonitrogenous and isolipidic diets (45% crude protein and 14% crude lipid) were formulated to contain graded DHA/EPA ratios of 0.65, 1.16, 1.60, 2.03 and 2.67. There were no differences in growth performance and feed utilization among treatments. Fish fed higher DHA/EPA ratios had higher malondialdehyde (MDA) contents in serum than lower ratios. Serum triacylglycerol (TAG) content was significantly higher in fish fed the lowest DHA/EPA ratio. Tissue fatty acid profiles reflected the diets despite down-regulation of LC-PUFA biosynthesis genes, fatty acyl desaturase 2 (fads2) and elongase of very long-chain fatty acids 5 (elovl5), by high DHA/EPA ratios. Expression of acetyl-CoA carboxylase alpha (accα) and carnitine palmitoyl transferase 1A (cpt1a) were up-regulated by high DHA/EPA ratio, whereas sterol regulatory element-binding protein-1 (srebp-1) and hormone-sensitive lipase (hsl) were down-regulated. Fatty acid synthase (fas), 6-phosphogluconate dehydrogenase (6pgd) and peroxisome proliferator-activated receptor alpha (pparα) showed highest expression in fish fed intermediate (1.16) DHA/EPA ratio. Overall, this study indicated that dietary DHA/EPA ratio affected fatty acid profiles and significantly influenced lipid metabolism including LC-PUFA biosynthesis and other anabolic and catabolic pathways, and also had impacts on antioxidant capacity and hematological characteristics.
- Published
- 2017
45. Correction: The Transcriptomic Signature of RacA Activation and Inactivation Provides New Insights into the Morphogenetic Network of Aspergillus niger
- Author
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Kwon, Min Jin, Nitsche, Benjamin M., Arentshorst, Mark, Jørgensen, Thomas R., Ram, Arthur F. J., and Meyer, Vera
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Multidisciplinary ,Science ,Correction ,Medicine - Published
- 2014
46. Comparative Analysis of Transcriptomes among Bombyx mori Strains and Sexes Reveals the Genes Regulating Melanic Morph and the Related Phenotypes
- Author
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He, Songzhen, primary, Tong, Xiaoling, additional, Lu, Kunpeng, additional, Lu, Yaru, additional, Luo, Jiangwen, additional, Yang, Wenhao, additional, Chen, Min, additional, Han, Min-jin, additional, Hu, Hai, additional, Lu, Cheng, additional, and Dai, Fangyin, additional
- Published
- 2016
- Full Text
- View/download PDF
47. The transcriptomic signature of RacA activation and inactivation provides new insights into the morphogenetic network of Aspergillus niger
- Author
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Arthur F. J. Ram, Min Jin Kwon, Benjamin M. Nitsche, Thomas R. Jørgensen, Mark Arentshorst, and Vera Meyer
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Science ,Mutant ,Biophysics ,Actin Filaments ,Yeast and Fungal Models ,GTPase ,Mycology ,Biology ,Microbiology ,Cell Growth ,Transcriptomes ,Fungal Proteins ,Model Organisms ,Genome Analysis Tools ,Molecular Cell Biology ,Morphogenesis ,Signaling in Cellular Processes ,Calcium Signaling ,Transcription factor ,Actin ,Calcium signaling ,Genetics ,Fungal protein ,Multidisciplinary ,Systems Biology ,Fungi ,Genomics ,Phenotype ,Signaling Cascades ,Cell biology ,Cell Motility ,Phospholipid Signaling Cascade ,Medicine ,Aspergillus niger ,Signal transduction ,Transcriptome ,Tor Signaling ,Signal Transduction ,Research Article - Abstract
RacA is the main Rho GTPase in Aspergillus niger regulating polarity maintenance via controlling actin dynamics. Both deletion and dominant activation of RacA (Rac(G18V)) provoke an actin localization defect and thereby loss of polarized tip extension, resulting in frequent dichotomous branching in the ΔracA strain and an apolar growing phenotype for Rac(G18V). In the current study the transcriptomics and physiological consequences of these morphological changes were investigated and compared with the data of the morphogenetic network model for the dichotomous branching mutant ramosa-1. This integrated approach revealed that polar tip growth is most likely orchestrated by the concerted activities of phospholipid signaling, sphingolipid signaling, TORC2 signaling, calcium signaling and CWI signaling pathways. The transcriptomic signatures and the reconstructed network model for all three morphology mutants (ΔracA, Rac(G18V), ramosa-1) imply that these pathways become integrated to bring about different physiological adaptations including changes in sterol, zinc and amino acid metabolism and changes in ion transport and protein trafficking. Finally, the fate of exocytotic (SncA) and endocytotic (AbpA, SlaB) markers in the dichotomous branching mutant ΔracA was followed, demonstrating that hyperbranching does not per se result in increased protein secretion.
- Published
- 2013
48. A Novel Canine Model of Acute Vertebral Artery Occlusion
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Jianping Jia, Weijian Jiang, Yunfeng Zhang, Hao Lin, Min Jin, Chengyong Xu, and Bin Du
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Male ,medicine.medical_specialty ,Mechanical Thrombolysis ,medicine.medical_treatment ,Vertebral artery ,lcsh:Medicine ,Arterial Occlusive Diseases ,Dogs ,Internal medicine ,medicine.artery ,medicine ,Animals ,Humans ,cardiovascular diseases ,Thrombus ,lcsh:Science ,Stroke ,Vertebral Artery ,Reduction (orthopedic surgery) ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Angiography ,medicine.disease ,Thrombosis ,Disease Models, Animal ,Acute Disease ,Cardiology ,Female ,lcsh:Q ,Radiology ,business ,Research Article - Abstract
Background The extended time window and theoretic reduction in hemorrhage make mechanical strategies an attractive approach for the treatment of patients with ischemic stroke. However, a limited availability of suitable animal models of cerebrovascular thrombosis has hampered the study of novel endovascular interventions. The aim of the present study was to develop a new technique for site-specific placement of a thrombus in a canine model that would allow for the evaluation of mechanical thrombectomy and clot retrieval methods and the visualization of thrombus dislocation or fragmentation during angiographic manipulation. Methods Angiography and embolization with a preformed thrombus were performed in 12 canines. Under fluoroscopic guidance, an embolism protection device (EPD) was anchored to the middle segment of the left vertebral artery (VA) via the left femoral arterial sheath. A preformed radiopaque clot was injected through the guide catheter into the left VA, via the contralateral femoral artery, proximal to the EPD. After 15 min of occlusion, the EPD was removed and persistent occlusion of the VA was documented angiographically. Results Angiography performed during the observation period confirmed the persistence of VA occlusion in each case, and displacement of the radiopaque clots did not occur during the 3-hour observation period. The technique allowed selective embolization of targeted vessels without thrombus fragmentation. Conclusion This study demonstrates, for the first time, a canine model of post-circulation embolism induced by autologous blood clot placement. This model can be rapidly formed and easily operated, and the site of thrombosis can be readily controlled.
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- 2015
49. Purinergic Activation of Ca2+-Permeable TRPV4 Channels Is Essential for Mechano-Sensitivity in the Aldosterone-Sensitive Distal Nephron
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Min Jin, Oleg Zaika, Mykola Mamenko, Roger G. O'Neil, and Oleh Pochynyuk
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Male ,Anatomy and Physiology ,030232 urology & nephrology ,lcsh:Medicine ,Stimulation ,Uridine Triphosphate ,Biochemistry ,Mechanotransduction, Cellular ,Ion Channels ,Receptors, Purinergic P2Y2 ,Transient receptor potential channel ,Mice ,0302 clinical medicine ,Adenosine Triphosphate ,Molecular Cell Biology ,lcsh:Science ,Aldosterone ,TRPC ,0303 health sciences ,Multidisciplinary ,Chemistry ,Purinergic receptor ,Purinergic signalling ,Immunohistochemistry ,Connecting tubule ,Signaling Cascades ,medicine.anatomical_structure ,Medicine ,Research Article ,Signal Transduction ,TRPV4 ,medicine.medical_specialty ,TRPV Cation Channels ,In Vitro Techniques ,Signaling Pathways ,Models, Biological ,03 medical and health sciences ,Internal medicine ,medicine ,Extracellular ,Calcium-Mediated Signal Transduction ,Animals ,Biology ,030304 developmental biology ,Renal Physiology ,lcsh:R ,Proteins ,Renal System ,Nephrons ,Mice, Inbred C57BL ,Endocrinology ,Calcium Signaling Cascade ,Biophysics ,lcsh:Q ,Calcium - Abstract
Mechanical forces are known to induce increases of [Ca(2+)](i) in the aldosterone-sensitive distal nephron (ASDN) cells to regulate epithelial transport. At the same time, mechanical stress stimulates ATP release from ASDN cells. In this study, we combined ratiometric Fura-2 based monitoring of [Ca(2+)](i) in freshly isolated split-opened ASDN with targeted deletion of P2Y2 and TRPV4 in mice to probe a role for purinergic signaling in mediating mechano-sensitive responses in ASDN cells. ATP application causes a reproducible transient Ca(2+) peak followed by a sustained plateau. Individual cells of the cortical collecting duct (CCD) and the connecting tubule (CNT) respond to purinergic stimulation with comparative elevations of [Ca(2+)](i). Furthermore, ATP-induced Ca(2+)-responses are nearly identical in both principal (AQP2-positive) and intercalated (AQP2-negative) cells as was confirmed using immunohistochemistry in split-opened ASDN. UTP application produces elevations of [Ca(2+)](i) similar to that observed with ATP suggesting a dominant role of P2Y2-like receptors in generation of [Ca(2+)](i) response. Indeed, genetic deletion of P2Y2 receptors decreases the magnitude of ATP-induced and UTP-induced Ca(2+) responses by more than 70% and 90%, respectively. Both intracellular and extracellular sources of Ca(2+) appeared to contribute to the generation of ATP-induced Ca(2+) response in ASDN cells. Importantly, flow- and hypotonic-induced Ca(2+) elevations are markedly blunted in P2Y2 -/- mice. We further demonstrated that activation of mechano-sensitive TRPV4 channel plays a major role in the sustained [Ca(2+)](i) elevation during purinergic stimulation. Consistent with this, ATP-induced Ca(2+) plateau are dramatically attenuated in TRV4 -/- mice. Inhibition of TRPC channels with 10 µM BTP2 also decreased ATP-induced Ca(2+) plateau whilst to a lower degree than that observed with TRPV4 inhibition/genetic deletion. We conclude that stimulation of purinergic signaling by mechanical stimuli leads to activation of TRPV4 and, to a lesser extent, TRPCs channels, and this is an important component of mechano-sensitive response of the ASDN.
- Published
- 2011
50. A rapid subtractive immunization method to prepare discriminatory monoclonal antibodies for food E. coli O157:H7 contamination
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Jun-Wen Li, Zhi-Qiang Shen, Xin-Wei Wang, Zhigang Qiu, Min Jin, Zhaoli Chen, and Jing Lang
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Bacterial Diseases ,Salmonella ,medicine.drug_class ,Immunology ,lcsh:Medicine ,Food Contamination ,medicine.disease_cause ,Monoclonal antibody ,Escherichia coli O157 ,Microbiology ,Mice ,Model Organisms ,Species Specificity ,medicine ,Methods ,Animals ,Shigella ,lcsh:Science ,Yersinia enterocolitica ,Pathogen ,Biology ,Immunoassay ,Multidisciplinary ,Hybridomas ,biology ,lcsh:R ,Antibodies, Monoclonal ,biology.organism_classification ,Virology ,Citrobacter freundii ,Infectious Diseases ,Immunologic Techniques ,Hybridoma technology ,Prokaryotic Models ,Medicine ,lcsh:Q ,Immunization ,Clinical Immunology ,Bacteria ,Research Article ,Biotechnology - Abstract
To detect food E. coli O157:H7 contamination rapidly and accurately, it is essential to prepare high specific monoclonal antibodies (mAbs) against the pathogen. Cyclophosphamide (Cy)-mediated subtractive immunization strategy was performed in mice to generate mAbs that react with E. coli O157:H7, but not with other affiliated bacteria. Specificity of 19 mAbs was evaluated by ELISA and/or dot-immunogold filtration assay (DIGFA). Immunogloubin typing, affinity and binding antigens of 5 selected mAbs were also analysed. MAbs 1D8, 4A7, 5A2 were found to have high reactivity with E. coli O157:H7 and no cross-reactivity with 80 other strains of bacteria including Salmonella sp., Shigella sp., Proteus sp., Yersinia enterocolitica, Staphylococcus aureus, Klebsiella pneumoniae, Citrobacter freundii and other non-E. coli O157:H7 enteric bacteria. Their ascetic titers reached 1:10(6) with E. coli O157:H7 and affinity constants ranged from 1.57 × 10(10) to 2.79 × 10(10) L/mol. The antigens recognized by them were different localized proteins. Furthermore, immune-colloidal gold probe coated with mAb 5A2 could specifically distinguish minced beef contaminated by E. coli O157:H7 from 84 other bacterial contaminations. The Cy-mediated subtractive immunization procedure coupled with hybridoma technology is a rapid and efficient approach to prepare discriminatory mAbs for detection of E. coli O157:H7 contamination in food.
- Published
- 2011
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