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Your search keyword '"Meneghetti, Giacomo"' showing total 3 results
Start Over Author "Meneghetti, Giacomo" Journal plos one
3 results on '"Meneghetti, Giacomo"'

1. Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development

Author

Skobo, Tatjana, primary, Benato, Francesca, additional, Grumati, Paolo, additional, Meneghetti, Giacomo, additional, Cianfanelli, Valentina, additional, Castagnaro, Silvia, additional, Chrisam, Martina, additional, Di Bartolomeo, Sabrina, additional, Bonaldo, Paolo, additional, Cecconi, Francesco, additional, and Valle, Luisa Dalla, additional

Published
2014
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2. Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development.

Author

Skobo, Tatjana, Benato, Francesca, Grumati, Paolo, Meneghetti, Giacomo, Cianfanelli, Valentina, Castagnaro, Silvia, Chrisam, Martina, Di Bartolomeo, Sabrina, Bonaldo, Paolo, Cecconi, Francesco, and Valle, Luisa Dalla

Subjects
ZEBRA danio, SKELETAL muscle, AUTOPHAGY, HOMEOSTASIS, PHENOTYPES, FISH embryology, FISH larvae, DISEASES
Abstract

The essential role of autophagy in muscle homeostasis has been clearly demonstrated by phenotype analysis of mice with muscle-specific inactivation of genes encoding autophagy-related proteins. Ambra1 is a key component of the Beclin 1 complex and, in zebrafish, it is encoded by two paralogous genes, ambra1a and ambra1b, both required for normal embryogenesis and larval development. In this study we focused on the function of Ambra1, a positive regulator of the autophagic process, during skeletal muscle development by means of morpholino (MO)-mediated knockdown and compared the phenotype of zebrafish Ambra1-depleted embryos with that of Ambra1gt/gt mouse embryos. Morphological analysis of zebrafish morphant embryos revealed that silencing of ambra1 impairs locomotor activity and muscle development, as well as myoD1 expression. Skeletal muscles in ATG-morphant embryos displayed severe histopathological changes and contained only small areas of organized myofibrils that were widely dispersed throughout the cell. Double knockdown of ambra1a and ambra1b resulted in a more severe phenotype whereas defects were much less evident in splice-morphants. The morphants phenotypes were effectively rescued by co-injection with human AMBRA1 mRNA. Together, these results indicate that ambra1a and ambra1b are required for the correct development and morphogenesis of skeletal muscle. [ABSTRACT FROM AUTHOR]

Published
2014
Full Text
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3. Zebrafish ambra1a and ambra1b Knockdown Impairs Skeletal Muscle Development

Author

Sabrina Di Bartolomeo, Valentina Cianfanelli, Francesca Benato, Paolo Grumati, Martina Chrisam, Silvia Castagnaro, Luisa Dalla Valle, Francesco Cecconi, Tatjana Skobo, Giacomo Meneghetti, Paolo Bonaldo, Skobo, Tatjana, Benato, Francesca, Grumati, Paolo, Meneghetti, Giacomo, Cianfanelli, Valentina, Castagnaro, Silvia, Chrisam, Martina, Di Bartolomeo, Sabrina, Bonaldo, Paolo, Cecconi, Francesco, and Dalla Valle, Luisa

Subjects
Embryo, Nonmammalian, Morpholino, Muscle Fibers, Skeletal, lcsh:Medicine, Muscle Development, Biochemistry, Morpholinos, Mice, Myosin, Morphogenesis, lcsh:Science, Zebrafish, Musculoskeletal System, In Situ Hybridization, Gene knockdown, Multidisciplinary, Birefringence, Muscles, Fishes, Gene Expression Regulation, Developmental, PAX7 Transcription Factor, Cell Differentiation, Animal Models, Phenotype, medicine.anatomical_structure, Osteichthyes, Gene Knockdown Techniques, Zebrafish Protein, embryonic structures, Vertebrates, Cytochemistry, Anatomy, Immunocytochemistry, Research Article, Settore BIO/06, animal structures, Movement, Molecular Probe Techniques, Mouse Models, Biology, Myosins, Research and Analysis Methods, Model Organisms, medicine, Animals, Molecular Biology Techniques, Muscle, Skeletal, Molecular Biology, Adaptor Proteins, Signal Transducing, Cell Proliferation, MyoD Protein, Animal, lcsh:R, Organisms, Skeletal muscle, Biology and Life Sciences, Morphant, Zebrafish Proteins, biology.organism_classification, Molecular biology, Probe Hybridization, lcsh:Q, Myofibril, Developmental Biology
Abstract

The essential role of autophagy in muscle homeostasis has been clearly demonstrated by phenotype analysis of mice with muscle-specific inactivation of genes encoding autophagy-related proteins. Ambra1 is a key component of the Beclin 1 complex and, in zebrafish, it is encoded by two paralogous genes, ambra1a and ambra1b, both required for normal embryogenesis and larval development. In this study we focused on the function of Ambra1, a positive regulator of the autophagic process, during skeletal muscle development by means of morpholino (MO)-mediated knockdown and compared the phenotype of zebrafish Ambra1-depleted embryos with that of Ambra1 gt/gt mouse embryos. Morphological analysis of zebrafish morphant embryos revealed that silencing of ambra1 impairs locomotor activity and muscle development, as well as myoD1 expression. Skeletal muscles in ATG-morphant embryos displayed severe histopathological changes and contained only small areas of organized myofibrils that were widely dispersed throughout the cell. Double knockdown of ambra1a and ambra1b resulted in a more severe phenotype whereas defects were much less evident in splice-morphants. The morphants phenotypes were effectively rescued by co-injection with human AMBRA1 mRNA. Together, these results indicate that ambra1a and ambra1b are required for the correct development and morphogenesis of skeletal muscle.

Published
2014

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