639 results on '"Matsumura A"'
Search Results
2. Relationship between maternal consumption of fermented foods and the development of the offspring at the age of 3 years: The Japan Environment and Children's Study.
- Author
-
Hiroko Hirai, Tomomi Tanaka, Kenta Matsumura, Akiko Tsuchida, Kei Hamazaki, Yuichi Adachi, Hidekuni Inadera, and Japan Environment and Children’s Study Group
- Subjects
Medicine ,Science - Abstract
BackgroundIt is well known that maternal diet affects the development of offspring. Herein, the relationship between maternal intake of fermented foods during pregnancy and offspring development was investigated.MethodsThe diet of 103,060 pregnant women at >4 months of gestation who were enrolled in the Japan Environment and Children's Study was analyzed. Their intake levels of fermented soybeans (miso and natto), yogurt, and cheese were investigated. The developmental status of the offspring at 3 years of age was assessed using the Ages and Stages Questionnaires (ASQ-3). Multivariable logistic regression analysis was performed to determine the risk of maternal intake levels of the fermented foods associated with subsequent developmental delay in the offspring.ResultsIntake of cheese was associated with a reduced risk of child developmental delay in all intake level groups from the second quartile onward. Intakes of miso and yogurt were associated with a reduced risk of developmental delay in communication skills in the fourth quartile. There was no association between intake of natto and developmental delay.ConclusionMaternal consumption of fermented foods during pregnancy may reduce the risk of later developmental delay in offspring. It is therefore important to review the mother's diet for fermented foods during pregnancy. However, further studies are warranted to evaluate the factors influencing the association between diet and offspring development.
- Published
- 2024
- Full Text
- View/download PDF
3. Specific pelvic shape in patients with developmental dysplasia of the hip on 3D morphometric homologous model analysis
- Author
-
Yui Sasaki, Daisuke Suzuki, Ryo Tokita, Hiroyuki Takashima, Hirofumi Matsumura, and Satoshi Nagoya
- Subjects
Medicine ,Science - Published
- 2024
4. Direct toxicity of cigarette smoke extract on cardiac function mediated by mitochondrial dysfunction in Sprague-Dawley rat ventricular myocytes and human induced pluripotent stem cell-derived cardiomyocytes.
- Author
-
Sakiko Matsumura, Jumpei Yasuda, Takuya Notomi, Yoshihiro Suzuki, I-Shan Chen, Daichi Murakami, Muneki Hotomi, and Tomoe Y Nakamura
- Subjects
Medicine ,Science - Abstract
Cigarette smoke has been recognized as a major risk factor for cardiovascular disease. However, its direct effects on rodent and human cardiomyocytes and its cellular mechanisms are not fully understood. In this study, we examined the direct effects of cigarette smoke extract (CSE) on contractile functions, intracellular Ca2+ dynamics, and mitochondrial function using cultured or freshly isolated rat ventricular myocytes and human induced pluripotent stem cell (iPS)-derived cardiomyocytes. In rat cardiomyocytes, CSE (≥0.1%) resulted in a time- and concentration-dependent cessation of spontaneous beating of cultured cardiomyocytes, eventually leading to cell death, which indicates direct toxicity. In addition, 1% CSE reduced contractile function of freshly isolated ventricular myocytes. Similar contractile dysfunction (declined spontaneous beating rate and contractility) was also observed in human iPS-derived cardiomyocytes. Regarding intracellular Ca2+ dynamics, 1% CSE increased the Ca2+ transient amplitude by greatly increasing systolic Ca2+ levels and slightly increasing diastolic Ca2+ levels. CSE also accelerated the decay of Ca2+ transients, and triggered spike-shaped Ca2+ transients in some cells. These results indicate that CSE causes abnormal Ca2+ dynamics in cardiomyocytes. Furthermore, CSE induced a cascade of mitochondrial dysfunctions, including increased mitochondrial reactive oxygen species, opening of mitochondrial permeability transition pore, reduction of mitochondrial membrane potential, and release of cytochrome c from mitochondria. These results suggest that CSE-induced contractile dysfunction and myocardial cell death is caused by abnormal Ca2+ dynamics and subsequent mitochondrial dysregulation, which would result in reduced bioenergetics and activation of cell death pathways.
- Published
- 2024
- Full Text
- View/download PDF
5. Political orientation of online media sources and reporting of Covid-19 vaccine myocarditis
- Author
-
Matsumura, Addison, primary, Garg, Ria, additional, Hussain, Muzna, additional, and Matsumura, Martin E., additional
- Published
- 2024
- Full Text
- View/download PDF
6. Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons.
- Author
-
Kazuhiko Nishida, Shinji Matsumura, Hitoshi Uchida, Manabu Abe, Kenji Sakimura, Tudor Constantin Badea, and Takuya Kobayashi
- Subjects
Medicine ,Science - Abstract
The spinal dorsal horn comprises heterogeneous neuronal populations, that interconnect with one another to form neural circuits modulating various types of sensory information. Decades of evidence has revealed that transcription factors expressed in each neuronal progenitor subclass play pivotal roles in the cell fate specification of spinal dorsal horn neurons. However, the development of subtypes of these neurons is not fully understood in more detail as yet and warrants the investigation of additional transcription factors. In the present study, we examined the involvement of the POU domain-containing transcription factor Brn3a in the development of spinal dorsal horn neurons. Analyses of Brn3a expression in the developing spinal dorsal horn neurons in mice demonstrated that the majority of the Brn3a-lineage neurons ceased Brn3a expression during embryonic stages (Brn3a-transient neurons), whereas a limited population of them continued to express Brn3a at high levels after E18.5 (Brn3a-persistent neurons). Loss of Brn3a disrupted the localization pattern of Brn3a-persistent neurons, indicating a critical role of this transcription factor in the development of these neurons. In contrast, Brn3a overexpression in Brn3a-transient neurons directed their localization in a manner similar to that in Brn3a-persistent neurons. Moreover, Brn3a-overexpressing neurons exhibited increased axonal extension to the ventral and ventrolateral funiculi, where the axonal tracts of Brn3a-persistent neurons reside. These results suggest that Brn3a controls the soma localization and axonal extension patterns of Brn3a-persistent spinal dorsal horn neurons.
- Published
- 2023
- Full Text
- View/download PDF
7. Probing the substrate binding modes and catalytic mechanisms of BLEG-1, a promiscuous B3 metallo-β-lactamase with glyoxalase II properties.
- Author
-
Shaw Xian Au, Azyyati Mohd Padzil, Noor Dina Muhd Noor, Hiroyoshi Matsumura, Raja Noor Zaliha Raja Abdul Rahman, and Yahaya M Normi
- Subjects
Medicine ,Science - Abstract
BLEG-1 from Bacillus lehensis G1 is an evolutionary divergent B3 metallo-β-lactamase (MBL) that exhibited both β-lactamase and glyoxalase II (GLXII) activities. Sequence, phylogeny, biochemical and structural relatedness of BLEG-1 to B3 MBL and GLXII suggested BLEG-1 might be an intermediate in the evolutionary path of B3 MBL from GLXII. The unique active site cavity of BLEG-1 that recognizes both β-lactam antibiotics and S-D-lactoylglutathione (SLG) had been postulated as the key factor for its dual activity. In this study, dynamic ensembles of BLEG-1 and its substrate complexes divulged conformational plasticity and binding modes of structurally distinct substrates to the enzyme, providing better insights into its structure-to-function relationship and enzymatic promiscuity. Our results highlight the flexible nature of the active site pocket of BLEG-1, which is governed by concerted loop motions involving loop7+α3+loop8 and loop12 around the catalytic core, thereby moulding the binding pocket and facilitate interactions of BLEG-1 with both ampicillin and SLG. The distribution of (i) predominantly hydrophobic amino acids in the N-terminal domain, and (ii) flexible amino acids with polar and/or charged side chains in both N- and C-termini provide additional advantages to BLEG-1 in confining the aromatic group of ampicillin, and polar groups of SLG, respectively. The importance of these residues for substrates binding was further confirmed by the reduction in MBL and GLXII activities upon alanine substitutions of Ile-10, Phe-57, Arg-94, Leu-95, and Arg-159. Based on molecular dynamics simulation, mutational, and biochemical data presented herein, the catalytic mechanisms of BLEG-1 toward the hydrolysis of β-lactams and SLG were proposed.
- Published
- 2023
- Full Text
- View/download PDF
8. Effects of elastase-induced emphysema on muscle and bone in mice.
- Author
-
Daichi Matsumura, Naoyuki Kawao, Katsumi Okumoto, Takashi Ohira, Yuya Mizukami, Masao Akagi, and Hiroshi Kaji
- Subjects
Medicine ,Science - Abstract
Chronic obstructive pulmonary disease (COPD) causes sarcopenia and osteoporosis. However, the mechanisms underlying muscle and bone loss as well as the interactions between muscle and bone in the COPD state remain unclear. Therefore, we herein investigated the effects of the COPD state on muscle and bone in mice intratracheally administered porcine pancreatic elastase (PPE). The intratracheal administration of PPE to mice significantly reduced trabecular bone mineral density (BMD), trabecular bone volume, trabecular number, cortical BMD and cortical area. It also significantly decreased grip strength, but did not affect muscle mass or the expression of myogenic differentiation-, protein degradation- or autophagy-related genes in the soleus and gastrocnemius muscles. Among the myokines examined, myostatin mRNA levels in the soleus muscles were significantly elevated in mice treated with PPE, and negatively related to grip strength, but not bone parameters, in mice treated with or without 2 U PPE in simple regression analyses. Grip strength positively related to bone parameters in mice treated with or without PPE. In conclusion, we showed that a PPE model of COPD in mice exerts dominant effects on bone rather than skeletal muscles. Increased myostatin expression in the soleus muscles of mice in the COPD state may negatively relate to a reduction in grip strength, but not bone loss.
- Published
- 2023
- Full Text
- View/download PDF
9. The influence of executive functions on eye-hand span and piano performance during sight-reading.
- Author
-
Kyoko Imai-Matsumura and Megumi Mutou
- Subjects
Medicine ,Science - Abstract
The ability to perform an unrehearsed piece of music, or sight-read, is a skill required by music performers. In sight-reading, the performer reads and plays the music simultaneously, which requires the coordination of visual, auditory, and motor processing. While performing, they display a characteristic called eye-hand span, in which the part of the score being looked at precedes the part being played. They must recognize, decipher, and process the score in the time between reading a note and playing it. An individual's executive function (EF) that control their cognition, emotions, and behavior may be involved in overseeing these individual movements. However, no study has investigated how EF is related to the eye-hand span and performance during sight-reading. Therefore, the purpose of this study is to clarify the relationships among EF, eye-hand span, and piano performance. Thirty-nine Japanese pianists and college students aspiring to be pianists with an average of 33.3 years of experience participated in this study. They performed sight-reading of two types of music scores with different difficulty levels while their eye movements were measured using an eye tracker to obtain their eye-hand span. The core EFs of inhibition, working memory, and shifting, were measured directly for each participant. Piano performance was evaluated by two pianists who did not participate in the study. Structural equation modeling was used to analyze the results. The results showed that auditory working memory predicted eye-hand span (β = .73, p < .001 in easy score; β = .65, p < .001 in difficult score), and eye-hand span predicted performance (β = .57, p < .001 in easy score; β = .56, p < .001 in difficult score). Auditory working memory did not directly affect performance, but through eye-hand span. The eye-hand span for easy scores was significantly greater than that for difficult scores. Furthermore, in a difficult music score, the shifting ability predicted higher piano performance. These suggest that the input of notes from the eyes becomes sound in the brain and activates the auditory working memory, which is then transmitted to finger movement, resulting in piano performance. In addition, it was suggested that shifting ability is also needed to perform difficult scores.
- Published
- 2023
- Full Text
- View/download PDF
10. Histology of metastatic colorectal cancer in a lymph node.
- Author
-
Shozo Yokoyama, Takashi Watanabe, Yoichi Fujita, Shuichi Matsumura, Katsuya Ueda, Shotaro Nagano, Ikuharu Kinoshita, Daisuke Murakami, Hirotaka Tabata, Toshiaki Tsuji, Satoru Ozawa, Takuya Tamaki, Yoshihiro Nakatani, and Masami Oka
- Subjects
Medicine ,Science - Abstract
BackgroundA primary colorectal cancer (CRC) tumor can contain heterogeneous cancer cells. As clones of cells with different properties metastasize to lymph nodes (LNs), they could show different morphologies. Cancer histologies in LNs of CRC remains to be described.MethodsOur study enrolled 318 consecutive patients with CRC who underwent primary tumor resection with lymph node dissection between January 2011 and June 2016. 119 (37.4%) patients who had metastatic LNs (mLNs) were finally included in this study. Cancer histologies in LNs were classified and compared with pathologically diagnosed differentiation in the primary lesion. The association between histologies in lymph node metastasis (LNM) and prognosis in patients with CRC was investigated.ResultsThe histologies of the cancer cells in the mLNs were classified into four types: tubular, cribriform, poorly differentiated, and mucinous. Same degree of pathologically diagnosed differentiation in the primary tumor produced various histological types in LNM. In Kaplan-Meier analysis, prognosis was worse in CRC patients with moderately differentiated adenocarcinoma who had at least some mLN also showing cribriform carcinoma than for those whose mLNs all showed tubular carcinoma.ConclusionsHistology in LNM from CRC might indicate the heterogeneity and malignant phenotype of the disease.
- Published
- 2023
- Full Text
- View/download PDF
11. In vitro spermatogenesis in isolated seminiferous tubules of immature mice.
- Author
-
Xuemin Feng, Takafumi Matsumura, Yuki Yamashita, Takuya Sato, Kiyoshi Hashimoto, Hisakazu Odaka, Yoshinori Makino, Yuki Okada, Hiroko Nakamura, Hiroshi Kimura, Teruo Fujii, and Takehiko Ogawa
- Subjects
Medicine ,Science - Abstract
Mouse spermatogenesis, from spermatogonial stem cell proliferation to sperm formation, can be reproduced in vitro by culturing testis tissue masses of neonatal mice. However, it remains to be determined whether this method is also applicable when testis tissues are further divided into tiny fragments, such as segments of the seminiferous tubule (ST), a minimal anatomical unit for spermatogenesis. In this study, we investigated this issue using the testis of an Acrosin-GFP/Histone H3.3-mCherry (Acr/H3) double-transgenic mouse and monitored the expression of GFP and mCherry as indicators of spermatogenic progression. Initially, we noticed that the cut and isolated stretches of ST shrunk rapidly and conglomerated. We therefore maintained the isolation of STs in two ways: segmental isolation without truncation or embedding in soft agarose. In both cases, GFP expression was observed by fluorescence microscopy. By whole-mount immunochemical staining, meiotic spermatocytes and round and elongating spermatids were identified as Sycp3-, crescent-form GFP-, and mCherry-positive cells, respectively. Although the efficiency was significantly lower than that with tissue mass culture, we clearly showed that spermatogenesis can be induced up to the elongating spermatid stage even when the STs were cut into short segments and cultured in isolation. In addition, we demonstrated that lowered oxygen tension was favorable for spermatogenesis both for meiotic progression and for producing elongating spermatids in isolated STs. Culturing isolated STs rather than tissue masses is advantageous for explicitly assessing the various environmental parameters that influence the progression of spermatogenesis.
- Published
- 2023
- Full Text
- View/download PDF
12. Mixbiotic society measures: Assessment of community well-going as living system.
- Author
-
Kato, Takeshi, Miyakoshi, Jyunichi, Matsumura, Tadayuki, Mine, Ryuji, Mizuno, Hiroyuki, and Deguchi, Yasuo
- Subjects
SOCIAL disorganization ,SOCIAL network analysis ,VIRTUAL communities ,EUCLIDEAN distance ,SOCIAL isolation - Abstract
Social isolation and fragmentation represent global challenges, with the former stemming from a lack of interaction and the latter from exclusive mobs—both rooted in communication issues. Addressing these challenges, the philosophical realm introduces the concept of the "mixbiotic society." In this framework, individuals with diverse freedoms and values mix together in physical proximity with diverse mingling, recognizing their respective "fundamental incapacities" and uniting in solidarity. This study aims to provide novel measures to balance freedom and solidarity, specifically the intermediate between isolation and mobbing, within a mixbiotic society. To achieve this, we introduce simplified measures to evaluate dynamic communication patterns. These measures complement traditional social network analysis of static structures and conventional entropy-based assessments of dynamic patterns. Our specific hypothesis posits that the measures corresponding to four distinct phases are established by representing communication patterns as multidimensional vectors. These measures include the mean of Euclidean distance to quantify "mobism" for fragmentation, the relative distance change for "atomism" indicating isolation, and a composite measure derived from multiplying the mean and variance of cosine similarity for "mixism," reflecting the well-going state of a mixbiotic society. Additionally, nearly negligible measures correspond to "nihilism." Through the evaluation of seven real-society datasets (high school, primary school, workplace, village, conference, online community, and email), we demonstrate the utility of the "mixism" measure in assessing freedom and solidarity in society. These measures can be employed to typify communities on a radar chart and a communication trajectory graph. The superiority of the measures lies in their ability to evaluate dynamic patterns, ease of calculation, and easily interpretable meanings compared to conventional analyses. As a future development, alongside additional validation using diverse datasets, the mixbiotic society measures will be employed to analyze social issues and applied in the fields of digital democracy and platform cooperativism. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
13. How do students study in STEM courses? Findings from a light-touch intervention and its relevance for underrepresented students.
- Author
-
Rodriguez, Fernando, Rivas, Mariela J, Matsumura, Lani H, Warschauer, Mark, and Sato, Brian K
- Subjects
Humans ,Learning ,Biology ,Mathematics ,Minority Groups ,Curriculum ,Students ,Computer-Assisted Instruction ,Technology ,Adolescent ,Female ,Male ,Young Adult ,General Science & Technology ,MD Multidisciplinary - Abstract
With the nationwide emphasis on improving outcomes for STEM undergraduates, it is important that we not only focus on modifying classroom instruction, but also provide students with the tools to maximize their independent learning time. There has been considerable work in laboratory settings examining two beneficial practices for enhancing learning: spacing and self-testing. In the current study, we examine biology students' study practices, particularly in the context of these two behaviors. We specifically investigate whether a light-touch study skills intervention focused on encouraging spacing and self-testing practices impacted their utilization. Based on pre- and post-course surveys, we found that students report utilizing both beneficial and ineffective study practices and confirm that usage of spacing and self-testing correlates with a higher course grade. We also found that students in the section of the course which received the study skills intervention were more likely to report continued use or adoption of spacing and self-testing compared to students in control sections without the intervention. Surprisingly, we found that underrepresented minorities (URMs) under-utilize self-testing, and that our intervention helped to partially ameliorate this gap. Additionally, we found that URMs who reported self-testing earned similar course grades compared to non-URMs who also self-tested, but that there was a much larger drop in performance for URMs who did not self-test relative to non-URMs who also did not self-test. Overall, we would encourage instructors to dedicate class time towards discussing the merits of beneficial study practices, especially for students that have historically underperformed in STEM disciplines.
- Published
- 2018
14. Direct toxicity of cigarette smoke extract on cardiac function mediated by mitochondrial dysfunction in Sprague-Dawley rat ventricular myocytes and human induced pluripotent stem cell-derived cardiomyocytes
- Author
-
Matsumura, Sakiko, primary, Yasuda, Jumpei, additional, Notomi, Takuya, additional, Suzuki, Yoshihiro, additional, Chen, I-Shan, additional, Murakami, Daichi, additional, Hotomi, Muneki, additional, and Nakamura, Tomoe Y., additional
- Published
- 2024
- Full Text
- View/download PDF
15. Relationship between maternal consumption of fermented foods and the development of the offspring at the age of 3 years: The Japan Environment and Children's Study.
- Author
-
Hirai, Hiroko, Tanaka, Tomomi, Matsumura, Kenta, Tsuchida, Akiko, Hamazaki, Kei, Adachi, Yuichi, and Inadera, Hidekuni
- Subjects
FOOD consumption ,FERMENTED foods ,YOGURT ,DEVELOPMENTAL delay ,LOGISTIC regression analysis ,PREGNANT women ,FOOD fermentation ,PREGNANCY ,COMMUNICATIVE competence - Abstract
Background: It is well known that maternal diet affects the development of offspring. Herein, the relationship between maternal intake of fermented foods during pregnancy and offspring development was investigated. Methods: The diet of 103,060 pregnant women at >4 months of gestation who were enrolled in the Japan Environment and Children's Study was analyzed. Their intake levels of fermented soybeans (miso and natto), yogurt, and cheese were investigated. The developmental status of the offspring at 3 years of age was assessed using the Ages and Stages Questionnaires (ASQ-3). Multivariable logistic regression analysis was performed to determine the risk of maternal intake levels of the fermented foods associated with subsequent developmental delay in the offspring. Results: Intake of cheese was associated with a reduced risk of child developmental delay in all intake level groups from the second quartile onward. Intakes of miso and yogurt were associated with a reduced risk of developmental delay in communication skills in the fourth quartile. There was no association between intake of natto and developmental delay. Conclusion: Maternal consumption of fermented foods during pregnancy may reduce the risk of later developmental delay in offspring. It is therefore important to review the mother's diet for fermented foods during pregnancy. However, further studies are warranted to evaluate the factors influencing the association between diet and offspring development. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
16. Specific pelvic shape in patients with developmental dysplasia of the hip on 3D morphometric homologous model analysis.
- Author
-
Sasaki, Yui, Suzuki, Daisuke, Tokita, Ryo, Takashima, Hiroyuki, Matsumura, Hirofumi, and Nagoya, Satoshi
- Subjects
DYSPLASIA ,TRIANGLES ,ANATOMICAL planes ,PRINCIPAL components analysis ,RECEIVER operating characteristic curves ,PELVIS - Abstract
Purpose: To clarify the morphological factors of the pelvis in patients with developmental dysplasia of the hip (DDH), three-dimensional (3D) pelvic morphology was analyzed using a template-fitting technique. Methods: Three-dimensional pelvic data of 50 patients with DDH (DDH group) and 3D pelvic data of 50 patients without obvious pelvic deformity (Normal group) were used. All patients were female. A template model was created by averaging the normal pelvises into a symmetrical and isotropic mesh. Next, 100 homologous models were generated by fitting the pelvic data of each group of patients to the template model. Principal component analysis was performed on the coordinates of each vertex (15,235 vertices) of the pelvic homologous model. In addition, a receiver-operating characteristic (ROC) curve was calculated from the sensitivity of DDH positivity for each principal component, and principal components for which the area under the curve was significantly large were extracted (p<0.05). Finally, which components of the pelvic morphology frequently seen in DDH patients are related to these extracted principal components was evaluated. Results: The first, third, and sixth principal components showed significantly larger areas under the ROC curves. The morphology indicated by the first principal component was associated with a decrease in coxal inclination in both the coronal and horizontal planes. The third principal component was related to the sacral inclination in the sagittal plane. The sixth principal component was associated with narrowing of the superior part of the pelvis. Conclusion: The most important factor in the difference between normal and DDH pelvises was the change in the coxal angle in both the coronal and horizontal planes. That is, in the anterior and superior views, the normal pelvis is a triangle, whereas in DDH, it was more like a quadrilateral. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
17. Threshold of increase in oxygen demand to predict mechanical ventilation use in novel coronavirus disease 2019: A retrospective cohort study incorporating restricted cubic spline regression.
- Author
-
Ryo Yamamoto, Ryo Takemura, Asako Yamamoto, Kazuki Matsumura, Daiki Kaito, Koichiro Homma, Michihiko Wada, Junichi Sasaki, and Keio Donner Project
- Subjects
Medicine ,Science - Abstract
BackgroundRapid deterioration of oxygenation occurs in novel coronavirus disease 2019 (COVID-19), and prediction of mechanical ventilation (MV) is needed for allocation of patients to intensive care unit. Since intubation is usually decided based on varying clinical conditions, such as required oxygen changes, we aimed to elucidate thresholds of increase in oxygen demand to predict MV use within 12 h.MethodsA single-center retrospective cohort study using data between January 2020 and January 2021was conducted. Data were retrieved from the hospital data warehouse. Adult patients diagnosed with COVID-19 with a positive polymerase chain reaction (PCR) who needed oxygen during admission were included. Hourly increments in oxygen demand were calculated using two consecutive oxygen values. Covariates were selected from measurements at the closest time points of oxygen data. Prediction of MV use within 12 h by required oxygen changes was evaluated with the area under the receiver operating curves (AUCs). A threshold for increased MV use risk was obtained from restricted cubic spline curves.ResultsAmong 66 eligible patients, 1835 oxygen data were analyzed. The AUC was 0.756 for predicting MV by oxygen demand changes, 0.888 by both amounts and changes in oxygen, and 0.933 by the model adjusted with respiratory rate, PCR quantification cycle (Ct), and days from PCR. The threshold of increments of required oxygen was identified as 0.44 L/min/h and the probability of MV use linearly increased afterward. In subgroup analyses, the threshold was lower (0.25 L/min/h) when tachypnea or frequent respiratory distress existed, whereas it was higher (1.00 L/min/h) when viral load is low (Ct ≥20 or days from PCR >7 days).ConclusionsHourly changes in oxygen demand predicted MV use within 12 h, with a threshold of 0.44 L/min/h. This threshold was lower with an unstable respiratory condition and higher with a low viral load.
- Published
- 2022
- Full Text
- View/download PDF
18. Brn3a controls the soma localization and axonal extension patterns of developing spinal dorsal horn neurons
- Author
-
Nishida, Kazuhiko, primary, Matsumura, Shinji, additional, Uchida, Hitoshi, additional, Abe, Manabu, additional, Sakimura, Kenji, additional, Badea, Tudor Constantin, additional, and Kobayashi, Takuya, additional
- Published
- 2023
- Full Text
- View/download PDF
19. Probing the substrate binding modes and catalytic mechanisms of BLEG-1, a promiscuous B3 metallo-β-lactamase with glyoxalase II properties
- Author
-
Au, Shaw Xian, primary, Mohd Padzil, Azyyati, additional, Muhd Noor, Noor Dina, additional, Matsumura, Hiroyoshi, additional, Raja Abdul Rahman, Raja Noor Zaliha, additional, and Normi, Yahaya M., additional
- Published
- 2023
- Full Text
- View/download PDF
20. Helicobacter pylori cholesteryl α-glucosides contribute to its pathogenicity and immune response by natural killer T cells.
- Author
-
Ito, Yuki, Vela, Jose Luis, Matsumura, Fumiko, Hoshino, Hitomi, Tyznik, Aaron, Lee, Heeseob, Girardi, Enrico, Zajonc, Dirk M, Liddington, Robert, Kobayashi, Motohiro, Bao, Xingfeng, Bugaytsova, Jeanna, Borén, Thomas, Jin, Rongsheng, Zong, Yinong, Seeberger, Peter H, Nakayama, Jun, Kronenberg, Mitchell, and Fukuda, Minoru
- Subjects
Th1 Cells ,Th2 Cells ,Animals ,Mice ,Knockout ,Humans ,Mice ,Helicobacter pylori ,Helicobacter Infections ,Gastritis ,Atrophic ,Glucosides ,Phagocytosis ,Adolescent ,Adult ,Aged ,Aged ,80 and over ,Middle Aged ,Child ,Female ,Male ,Natural Killer T-Cells ,General Science & Technology - Abstract
Approximately 10-15% of individuals infected with Helicobacter pylori will develop ulcer disease (gastric or duodenal ulcer), while most people infected with H. pylori will be asymptomatic. The majority of infected individuals remain asymptomatic partly due to the inhibition of synthesis of cholesteryl α-glucosides in H. pylori cell wall by α1,4-GlcNAc-capped mucin O-glycans, which are expressed in the deeper portion of gastric mucosa. However, it has not been determined how cholesteryl α-glucosyltransferase (αCgT), which forms cholesteryl α-glucosides, functions in the pathogenesis of H. pylori infection. Here, we show that the activity of αCgT from H. pylori clinical isolates is highly correlated with the degree of gastric atrophy. We investigated the role of cholesteryl α-glucosides in various aspects of the immune response. Phagocytosis and activation of dendritic cells were observed at similar degrees in the presence of wild-type H. pylori or variants harboring mutant forms of αCgT showing a range of enzymatic activity. However, cholesteryl α-glucosides were recognized by invariant natural killer T (iNKT) cells, eliciting an immune response in vitro and in vivo. Following inoculation of H. pylori harboring highly active αCgT into iNKT cell-deficient (Jα18(-/-)) or wild-type mice, bacterial recovery significantly increased in Jα18(-/-) compared to wild-type mice. Moreover, cytokine production characteristic of Th1 and Th2 cells dramatically decreased in Jα18(-/-) compared to wild-type mice. These findings demonstrate that cholesteryl α-glucosides play critical roles in H. pylori-mediated gastric inflammation and precancerous atrophic gastritis.
- Published
- 2013
21. The influence of executive functions on eye-hand span and piano performance during sight-reading
- Author
-
Imai-Matsumura, Kyoko, primary and Mutou, Megumi, additional
- Published
- 2023
- Full Text
- View/download PDF
22. Histology of metastatic colorectal cancer in a lymph node
- Author
-
Yokoyama, Shozo, primary, Watanabe, Takashi, additional, Fujita, Yoichi, additional, Matsumura, Shuichi, additional, Ueda, Katsuya, additional, Nagano, Shotaro, additional, Kinoshita, Ikuharu, additional, Murakami, Daisuke, additional, Tabata, Hirotaka, additional, Tsuji, Toshiaki, additional, Ozawa, Satoru, additional, Tamaki, Takuya, additional, Nakatani, Yoshihiro, additional, and Oka, Masami, additional
- Published
- 2023
- Full Text
- View/download PDF
23. In vitro spermatogenesis in isolated seminiferous tubules of immature mice
- Author
-
Feng, Xuemin, primary, Matsumura, Takafumi, additional, Yamashita, Yuki, additional, Sato, Takuya, additional, Hashimoto, Kiyoshi, additional, Odaka, Hisakazu, additional, Makino, Yoshinori, additional, Okada, Yuki, additional, Nakamura, Hiroko, additional, Kimura, Hiroshi, additional, Fujii, Teruo, additional, and Ogawa, Takehiko, additional
- Published
- 2023
- Full Text
- View/download PDF
24. Histone methyltransferase SUV39H1 regulates the Golgi complex via the nuclear envelope-spanning LINC complex.
- Author
-
Nishino, Miyu, Imaizumi, Hiromasa, Yokoyama, Yuhki, Katahira, Jun, Kimura, Hiroshi, Matsuura, Nariaki, and Matsumura, Miki
- Subjects
GOLGI apparatus ,NUCLEAR membranes ,CELL motility ,CELL migration ,METHYLTRANSFERASES ,MICROTUBULES ,CHROMATIN - Abstract
Cell motility is related to the higher-order structure of chromatin. Stimuli that induce cell migration change chromatin organization; such stimuli include elevated histone H3 lysine 9 trimethylation (H3K9me3). We previously showed that depletion of histone H3 lysine 9 methyltransferase, SUV39H1, suppresses directional cell migration. However, the molecular mechanism underlying this association between chromatin and cell migration remains elusive. The Golgi apparatus is a cell organelle essential for cell motility. In this study, we show that loss of H3K9 methyltransferase SUV39H1 but not SETDB1 or SETDB2 causes dispersion of the Golgi apparatus throughout the cytoplasm. The Golgi dispersion triggered by SUV39H1 depletion is independent of transcription, centrosomes, and microtubule organization, but is suppressed by depletion of any of the following three proteins: LINC complex components SUN2, nesprin-2, or microtubule plus-end-directed kinesin-like protein KIF20A. In addition, SUN2 is closely localized to H3K9me3, and SUV39H1 affects the mobility of SUN2 in the nuclear envelope. Further, inhibition of cell motility caused by SUV39H1 depletion is restored by suppression of SUN2, nesprin-2, or KIF20A. In summary, these results show the functional association between chromatin organization and cell motility via the Golgi organization regulated by the LINC complex. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
25. Progression of microstructural deterioration in load-bearing immobilization osteopenia
- Author
-
Koseki, Hironobu, primary, Osaki, Makoto, additional, Honda, Yuichiro, additional, Sunagawa, Shinya, additional, Imai, Chieko, additional, Shida, Takayuki, additional, Matsumura, Umi, additional, Sakamoto, Junya, additional, Tomonaga, Iku, additional, Yokoo, Seiichi, additional, Mizukami, Satoshi, additional, and Okita, Minoru, additional
- Published
- 2022
- Full Text
- View/download PDF
26. Integration of bioassay and non-target metabolite analysis of tomato reveals that β-carotene and lycopene activate the adiponectin signaling pathway, including AMPK phosphorylation
- Author
-
Mohri, Shinsuke, primary, Takahashi, Haruya, additional, Sakai, Maiko, additional, Waki, Naoko, additional, Takahashi, Shingo, additional, Aizawa, Koichi, additional, Suganuma, Hiroyuki, additional, Ara, Takeshi, additional, Sugawara, Tatsuya, additional, Shibata, Daisuke, additional, Matsumura, Yasuki, additional, Goto, Tsuyoshi, additional, and Kawada, Teruo, additional
- Published
- 2022
- Full Text
- View/download PDF
27. Effects of elastase-induced emphysema on muscle and bone in mice.
- Author
-
Matsumura, Daichi, Kawao, Naoyuki, Okumoto, Katsumi, Ohira, Takashi, Mizukami, Yuya, Akagi, Masao, and Kaji, Hiroshi
- Subjects
- *
SOLEUS muscle , *BONE density , *CHRONIC obstructive pulmonary disease , *SKELETAL muscle , *GRIP strength , *CANCELLOUS bone - Abstract
Chronic obstructive pulmonary disease (COPD) causes sarcopenia and osteoporosis. However, the mechanisms underlying muscle and bone loss as well as the interactions between muscle and bone in the COPD state remain unclear. Therefore, we herein investigated the effects of the COPD state on muscle and bone in mice intratracheally administered porcine pancreatic elastase (PPE). The intratracheal administration of PPE to mice significantly reduced trabecular bone mineral density (BMD), trabecular bone volume, trabecular number, cortical BMD and cortical area. It also significantly decreased grip strength, but did not affect muscle mass or the expression of myogenic differentiation-, protein degradation- or autophagy-related genes in the soleus and gastrocnemius muscles. Among the myokines examined, myostatin mRNA levels in the soleus muscles were significantly elevated in mice treated with PPE, and negatively related to grip strength, but not bone parameters, in mice treated with or without 2 U PPE in simple regression analyses. Grip strength positively related to bone parameters in mice treated with or without PPE. In conclusion, we showed that a PPE model of COPD in mice exerts dominant effects on bone rather than skeletal muscles. Increased myostatin expression in the soleus muscles of mice in the COPD state may negatively relate to a reduction in grip strength, but not bone loss. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
28. Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model
- Author
-
Matsumura, Goki, primary, Isayama, Noriko, additional, and Sato, Hideki, additional
- Published
- 2022
- Full Text
- View/download PDF
29. Disruption of CRTC1 and CRTC2 in Sim1 cells strongly increases high-fat diet intake in female mice but has a modest impact on male mice
- Author
-
Tanaka, Jin, primary, Ishikawa, Fuka, additional, Jinno, Tomoki, additional, Miyakita, Motoki, additional, Miyamori, Haruka, additional, Sasaki, Tsutomu, additional, Yokokawa, Takumi, additional, Goto, Tsuyoshi, additional, Inoue, Kazuo, additional, and Matsumura, Shigenobu, additional
- Published
- 2022
- Full Text
- View/download PDF
30. Progression of microstructural deterioration in load-bearing immobilization osteopenia
- Author
-
Hironobu Koseki, Makoto Osaki, Yuichiro Honda, Shinya Sunagawa, Chieko Imai, Takayuki Shida, Umi Matsumura, Junya Sakamoto, Iku Tomonaga, Seiichi Yokoo, Satoshi Mizukami, and Minoru Okita
- Subjects
Male ,Weight-Bearing ,Immobilization ,Bone Diseases, Metabolic ,Multidisciplinary ,Bone Density ,Animals ,X-Ray Microtomography ,Rats, Wistar ,Rats - Abstract
Purpose Immobilization osteopenia is a major healthcare problem in clinical and social medicine. However, the mechanisms underlying this bone pathology caused by immobilization under load-bearing conditions are not yet fully understood. This study aimed to evaluate sequential changes to the three-dimensional microstructure of bone in load-bearing immobilization osteopenia using a fixed-limb rat model. Materials and method Eight-week-old specific-pathogen-free male Wistar rats were divided into an immobilized group and a control group (n = 60 each). Hind limbs in the immobilized group were fixed using orthopedic casts with fixation periods of 1, 2, 4, 8, and 12 weeks. Feeding and weight-bearing were freely permitted. Length of the right femur was measured after each fixation period and bone microstructure was analyzed by micro-computed tomography. The architectural parameters of cortical and cancellous bone were analyzed statistically. Results Femoral length was significantly shorter in the immobilized group than in the control group after 2 weeks. Total area and marrow area were significantly lower in the immobilized group than in the control group from 1 to 12 weeks. Cortical bone area, cortical thickness, and polar moment of inertia decreased significantly after 2 weeks. Some cancellous bone parameters showed osteoporotic changes at 2 weeks after immobilization and the gap with the control group widened as the fixation period extended (P < 0.05). Conclusion The present results indicate that load-bearing immobilization triggers early deterioration of microstructure in both cortical and cancellous bone after 2 weeks., PLoS ONE, 17(11), art. no. e0275439; 2022
- Published
- 2022
31. Teachers’ visual processing of children’s off-task behaviors in class: A comparison between teachers and student teachers
- Author
-
Shinoda, Hirofumi, primary, Yamamoto, Tsuyoshi, additional, and Imai-Matsumura, Kyoko, additional
- Published
- 2021
- Full Text
- View/download PDF
32. Percutaneous coronary intervention using new-generation drug-eluting stents versus coronary arterial bypass grafting in stable patients with multi-vessel coronary artery disease: From the CREDO-Kyoto PCI/CABG registry Cohort-3
- Author
-
Watanabe, Hiroki, Yamamoto, Ko, Shiomi, Hiroki, Morimoto, Takeshi, Kato, Eri, Matsumura, Yukiko, Nakatsuma, Kenji, Takeji, Yasuaki, Yaku, Hidenori, Yamamoto, Erika, Yamashita, Yugo, Yoshikawa, Yusuke, Fuki, Masayuki, Yamaji, Kyohei, Ehara, Natsuhiko, Sakamoto, Hiroki, Imada, Kazuaki, Tada, Takeshi, Taniguchi, Ryoji, Nishikawa, Ryusuke, Tada, Tomohisa, Uegaito, Takashi, Ogawa, Tatsuya, Yamada, Miho, Takeda, Teruki, Eizawa, Hiroshi, Tamura, Nobushige, Tambara, Keiichi, Suwa, Satoru, Shirotani, Manabu, Tamura, Toshihiro, Inoko, Moriaki, Nishizawa, Junichiro, Natsuaki, Masahiro, Sakai, Hiroshi, Yamamoto, Takashi, Kanemitsu, Naoki, Ohno, Nobuhisa, Ishii, Katsuhisa, Marui, Akira, Tsuneyoshi, Hiroshi, Terai, Yasuhiko, Nakayama, Shogo, Yamazaki, Kazuhiro, Takahashi, Mamoru, Tamura, Takashi, Esaki, Jiro, Miki, Shinji, Onodera, Tomoya, Mabuchi, Hiroshi, Furukawa, Yutaka, Tanaka, Masaru, Komiya, Tatsuhiko, Soga, Yoshiharu, Hanyu, Michiya, Ando, Kenji, Kadota, Kazushige, Minatoya, Kenji, Nakagawa, Yoshihisa, and Kimura, Takeshi
- Subjects
Ischemic stroke ,Multidisciplinary ,Revascularization ,Coronary angioplasty ,Coronary artery bypass grafting ,Drug-Eluting Stents ,Coronary Artery Disease ,Coronary revascularization ,Cardiovascular disease risk ,Stroke ,Myocardial infarction ,Percutaneous Coronary Intervention ,Humans ,Registries ,Coronary Artery Bypass ,Coronary stenting - Abstract
Aims There is a scarcity of studies comparing percutaneous coronary intervention (PCI) using new-generation drug-eluting stents (DES) with coronary artery bypass grafting (CABG) in patients with multi-vessel coronary artery disease. Methods and results The CREDO-Kyoto PCI/CABG registry Cohort-3 enrolled 14927 consecutive patients who underwent first coronary revascularization with PCI or isolated CABG between January 2011 and December 2013. The current study population consisted of 2464 patients who underwent multi-vessel coronary revascularization including revascularization of left anterior descending coronary artery (LAD) either with PCI using new-generation DES (N = 1565), or with CABG (N = 899). Patients in the PCI group were older and more often had severe frailty, but had less complex coronary anatomy, and less complete revascularization than those in the CABG group. Cumulative 5-year incidence of a composite of all-cause death, myocardial infarction or stroke was not significantly different between the 2 groups (25.0% versus 21.5%, P = 0.15). However, after adjusting confounders, the excess risk of PCI relative to CABG turned to be significant for the composite endpoint (HR 1.27, 95%CI 1.04–1.55, P = 0.02). PCI as compared with CABG was associated with comparable adjusted risk for all-cause death (HR 1.22, 95%CI 0.96–1.55, P = 0.11), and stroke (HR 1.17, 95%CI 0.79–1.73, P = 0.44), but with excess adjusted risk for myocardial infarction (HR 1.58, 95%CI 1.05–2.39, P = 0.03), and any coronary revascularization (HR 2.66, 95%CI 2.06–3.43, P Conclusions In this observational study, PCI with new-generation DES as compared with CABG was associated with excess long-term risk for major cardiovascular events in patients who underwent multi-vessel coronary revascularization including LAD.
- Published
- 2022
33. Integration of bioassay and non-target metabolite analysis of tomato reveals that β-carotene and lycopene activate the adiponectin signaling pathway, including AMPK phosphorylation
- Author
-
Shinsuke Mohri, Haruya Takahashi, Maiko Sakai, Naoko Waki, Shingo Takahashi, Koichi Aizawa, Hiroyuki Suganuma, Takeshi Ara, Tatsuya Sugawara, Daisuke Shibata, Yasuki Matsumura, Tsuyoshi Goto, and Teruo Kawada
- Subjects
Lycopene ,Multidisciplinary ,Solanum lycopersicum ,Adenylate Kinase ,Biological Assay ,Calcium ,Adiponectin ,AMP-Activated Protein Kinases ,Phosphorylation ,RNA, Small Interfering ,Receptors, Adiponectin ,beta Carotene ,Signal Transduction - Abstract
Adiponectin, an adipokine, regulates glucose metabolism and insulin sensitivity through the adiponectin receptor (AdipoR). In this study, we searched for metabolites that activate the adiponectin signaling pathway from tomato (Solanum lycopersicu). Metabolites of mature tomato were separated into 55 fractions by liquid chromatography, and then each fraction was examined using the phosphorylation assay of AMP-protein kinase (AMPK) in C2C12 myotubes and in AdipoR-knockdown cells by small interfering RNA (siRNA). Several fractions showed AMPK phosphorylation in C2C12 myotubes and siRNA-mediated abrogation of the effect. Non-targeted metabolite analysis revealed the presence of 721 diverse metabolites in tomato. By integrating the activity of fractions on AMPK phosphorylation and the 721 metabolites based on their retention times of liquid chromatography, we performed a comprehensive screen for metabolites that possess adiponectin-like activity. As the screening suggested that the active fractions contained four carotenoids, we further analyzed β-carotene and lycopene, the major carotenoids of food. They induced AMPK phosphorylation via the AdipoR, Ca2+/calmodulin-dependent protein kinase kinase and Ca2+ influx, in addition to activating glucose uptake via AdipoR in C2C12 myotubes. All these events were characteristic adiponectin actions. These results indicated that the food-derived carotenoids, β-carotene and lycopene, activate the adiponectin signaling pathway, including AMPK phosphorylation.
- Published
- 2022
34. Demographic, clinical characteristics and treatment outcomes of immune-complex membranoproliferative glomerulonephritis and C3 glomerulonephritis in Japan: A retrospective analysis of data from the Japan Renal Biopsy Registry
- Author
-
Nakagawa, Naoki, primary, Mizuno, Masashi, additional, Kato, Sawako, additional, Maruyama, Shoichi, additional, Sato, Hiroshi, additional, Nakaya, Izaya, additional, Sugiyama, Hitoshi, additional, Fujimoto, Shouichi, additional, Miura, Kenichiro, additional, Matsumura, Chieko, additional, Gotoh, Yoshimitsu, additional, Suzuki, Hitoshi, additional, Kuroki, Aki, additional, Yoshino, Atsunori, additional, Nakatani, Shinya, additional, Hiromura, Keiju, additional, Yamamoto, Ryohei, additional, Yokoyama, Hitoshi, additional, Narita, Ichiei, additional, and Isaka, Yoshitaka, additional
- Published
- 2021
- Full Text
- View/download PDF
35. Combining IL-6 and SARS-CoV-2 RNAaemia-based risk stratification for fatal outcomes of COVID-19
- Author
-
Saji, Ryo, primary, Nishii, Mototsugu, additional, Sakai, Kazuya, additional, Miyakawa, Kei, additional, Yamaoka, Yutaro, additional, Ban, Tatsuma, additional, Abe, Takeru, additional, Ohyama, Yutaro, additional, Nakajima, Kento, additional, Hiromi, Taro, additional, Matsumura, Reo, additional, Suzuki, Naoya, additional, Taniguchi, Hayato, additional, Otsuka, Tsuyoshi, additional, Oi, Yasufumi, additional, Ogawa, Fumihiro, additional, Uchiyama, Munehito, additional, Takahashi, Kohei, additional, Iwashita, Masayuki, additional, Kimura, Yayoi, additional, Fujii, Satoshi, additional, Furuya, Ryosuke, additional, Tamura, Tomohiko, additional, Ryo, Akihide, additional, and Takeuchi, Ichiro, additional
- Published
- 2021
- Full Text
- View/download PDF
36. On-admission SARS-CoV-2 RNAemia as a single potent predictive marker of critical condition development and mortality in COVID-19
- Author
-
Miki, Shoji, primary, Sasaki, Hiroaki, additional, Horiuchi, Hiroshi, additional, Miyata, Nobuyuki, additional, Yoshimura, Yukihiro, additional, Miyazaki, Kazuhito, additional, Matsumura, Takayuki, additional, Takahashi, Yoshimasa, additional, Suzuki, Tadaki, additional, Matano, Tetsuro, additional, Kawana-Tachikawa, Ai, additional, and Tachikawa, Natsuo, additional
- Published
- 2021
- Full Text
- View/download PDF
37. Evaluation method for cell-free in situ tissue-engineered vasculature monitoring: Proof of growth and development in a canine IVC model
- Author
-
Goki Matsumura, Noriko Isayama, and Hideki Sato
- Subjects
Dogs ,Multidisciplinary ,Tissue Engineering ,Animals ,Vena Cava, Inferior ,Growth and Development ,Hepatic Veins - Abstract
We previously developed a non-cell-dependent biodegradable scaffold to create in situ tissue-engineered vasculature (iTEV) and tested it in a canine inferior vena cava (IVC) model. As iTEV features change dramatically during tissue generation, practical, simple, and accurate methods to evaluate iTEV are needed. The present study examined the usefulness of a novel method to evaluate iTEV growth and remodeling according to a simple formula using angiography: hepatic vein (HV) index = (IVC–HV junction angle) ÷ (π × [minimal internal iTEV diameter ÷ 2]2). HV index strongly correlated with the pressure gradient across iTEV, which tended to improve during the tissue generation period up to 12 months post-implantation. Time-course changes in HV index reflected iTEV tissue development and in-vivo characteristics, such as hemodynamic congestion. In conclusion, HV index is useful to assess iTEV graft function because it represents both the morphometrics and hemodynamics of iTEV with only diagnostic imaging data.
- Published
- 2022
38. Demographic, clinical characteristics and treatment outcomes of immune-complex membranoproliferative glomerulonephritis and C3 glomerulonephritis in Japan: A retrospective analysis of data from the Japan Renal Biopsy Registry
- Author
-
Hitoshi Yokoyama, Chieko Matsumura, Hitoshi Sugiyama, Hitoshi Suzuki, Izaya Nakaya, Keiju Hiromura, Yoshitaka Isaka, Hiroshi Sato, Atsunori Yoshino, Yoshimitsu Gotoh, Shinya Nakatani, Masashi Mizuno, Ichiei Narita, Shoichi Maruyama, Ryohei Yamamoto, Shouichi Fujimoto, Sawako Kato, Kenichiro Miura, Aki Kuroki, and Naoki Nakagawa
- Subjects
Male ,C3 Glomerulonephritis ,Physiology ,Biopsy ,Complement System ,Fluorescent Antibody Technique ,Blood Pressure ,Antigen-Antibody Complex ,Kidney ,Vascular Medicine ,Biochemistry ,Steroid Therapy ,Glomerulonephritis ,Japan ,Immune Physiology ,Membranoproliferative glomerulonephritis ,Chronic Kidney Disease ,Medicine and Health Sciences ,Registries ,Multidisciplinary ,Proteinuria ,Immune System Proteins ,medicine.diagnostic_test ,Pharmaceutics ,Remission Induction ,Age Factors ,Middle Aged ,medicine.anatomical_structure ,Treatment Outcome ,Nephrology ,Medicine ,Female ,Renal biopsy ,medicine.symptom ,Anatomy ,Research Article ,Adult ,medicine.medical_specialty ,Adolescent ,Science ,Immunology ,Renal function ,Surgical and Invasive Medical Procedures ,Signs and Symptoms ,Drug Therapy ,Internal medicine ,medicine ,Renal Diseases ,Humans ,Aged ,Demography ,Retrospective Studies ,business.industry ,Biology and Life Sciences ,Proteins ,Renal System ,medicine.disease ,Immune System ,Clinical Medicine ,business ,Kidney disease ,Follow-Up Studies - Abstract
The reclassification of membranoproliferative glomerulonephritis (MPGN) into immune-complex MPGN (IC-MPGN) and C3 glomerulopathy (C3G) based on immunofluorescence findings in kidney biopsies has provided insights into these two distinct diseases. C3G is further classified into dense deposit disease and C3 glomerulonephritis (C3GN) based on electron micrographic findings. Although these diseases have poor outcomes, limited Japanese literature confined to small, single-center cohorts exist on these diseases. We retrospectively analyzed 81 patients with MPGN type I and III from 15 hospitals in the Japan Renal Biopsy Registry to compare demographic, clinical characteristics and treatment outcomes of patients with IC-MPGN to those with C3GN. Of the 81 patients reviewed by immunofluorescence findings in kidney biopsies, 67 patients had IC-MPGN and 14 patients had C3GN. Age at diagnosis and systolic and diastolic pressure were higher and proteinuria and impaired renal function were significantly more prevalent in patients with IC-MPGN than those with C3GN. About 80% of the patients in both groups were treated with immunosuppressive therapy. At last follow-up (median 4.8 years), complete remission rate of proteinuria was significantly higher in patients with C3GN (64.3%) than in those with IC-MPGN (29.9%; P = 0.015). The renal survival rate was lower in patients with IC-MPGN when compared to C3GN (73.1% vs. 100%; log-rank, P = 0.031). Systolic blood pressure and renal function at baseline were independent predictors of progression to end-stage kidney disease. The overall prognosis of patients with C3GN is more favorable than for patients with IC-MPGN.
- Published
- 2021
39. Preventive effects of nitrate-rich beetroot juice supplementation on monocrotaline-induced pulmonary hypertension in rats
- Author
-
Masashi Tawa, Yasuo Matsumura, Tatsuya Sawano, Rikako Nagata, Mamoru Ohkita, Yuiko Sumi, and Keisuke Nakagawa
- Subjects
0301 basic medicine ,Male ,Physiology ,medicine.medical_treatment ,Blood Pressure ,030204 cardiovascular system & hematology ,Beetroot Juice ,Biochemistry ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,0302 clinical medicine ,Nitrate ,Medicine and Health Sciences ,Ingestion ,Nitrite ,Pulmonary Arteries ,Saline ,Multidisciplinary ,Monocrotaline ,Chemistry ,Pharmaceutics ,Neurochemistry ,Arteries ,Body Fluids ,Fruit and Vegetable Juices ,Blood ,Physiological Parameters ,Physical Sciences ,Medicine ,Anatomy ,Neurochemicals ,Beta vulgaris ,Research Article ,medicine.medical_specialty ,Hypertension, Pulmonary ,Science ,Cardiac Hypertrophy ,Cardiology ,Nitric Oxide ,Blood Plasma ,Nitric oxide ,03 medical and health sciences ,Drug Therapy ,Right ventricular hypertrophy ,Internal medicine ,medicine ,Animals ,Nitrites ,Nitrates ,Body Weight ,Chemical Compounds ,Biology and Life Sciences ,medicine.disease ,Pulmonary hypertension ,Rats ,030104 developmental biology ,Endocrinology ,Dietary Supplements ,Cardiovascular Anatomy ,Blood Vessels ,Receptor Antagonist Therapy ,Neuroscience - Abstract
Beetroot (Beta vulgaris L.) has a high level of nitrate; therefore, its dietary intake could increase nitric oxide (NO) level in the body, possibly preventing the development of pulmonary hypertension (PH). In this study, we examined the effects of beetroot juice (BJ) supplementation on PH and the contribution of nitrate to such effects using a rat model of monocrotaline (MCT, 60 mg/kg s.c.)-induced PH. Rats were injected subcutaneously with saline or 60 mg/kg MCT and were sacrificed 28 days after the injection. In some rats injected with MCT, BJ was supplemented from the day of MCT injection to the day of sacrifice. First, MCT-induced right ventricular systolic pressure elevation, pulmonary arterial medial thickening and muscularization, and right ventricular hypertrophy were suppressed by supplementation with low-dose BJ (nitrate: 1.3 mmol/L) but not high-dose BJ (nitrate: 4.3 mmol/L). Of the plasma nitrite, nitrate, and their sum (NOx) levels, only the nitrate levels were found to be increased by the high-dose BJ supplementation. Second, in order to clarify the possible involvement of nitrate in the preventive effects of BJ on PH symptoms, the effects of nitrate-rich BJ (nitrate: 0.9 mmol/L) supplementation were compared with those of the nitrate-depleted BJ. While the former exerted preventive effects on PH symptoms, such effects were not observed in rats supplemented with nitrate-depleted BJ. Neither supplementation with nitrate-rich nor nitrate-depleted BJ affected plasma nitrite, nitrate, and NOx levels. These findings suggest that a suitable amount of BJ ingestion, which does not affect systemic NO levels, can prevent the development of PH in a nitrate-dependent manner. Therefore, BJ could be highly useful as a therapy in patients with PH.
- Published
- 2021
40. On-admission SARS-CoV-2 RNAemia as a single potent predictive marker of critical condition development and mortality in COVID-19
- Author
-
Ai Kawana-Tachikawa, Tetsuro Matano, Yukihiro Yoshimura, Yoshimasa Takahashi, Natsuo Tachikawa, Takayuki Matsumura, Hiroaki Sasaki, Hiroshi Horiuchi, Kazuhito Miyazaki, Tadaki Suzuki, Nobuyuki Miyata, and Shoji Miki
- Subjects
RNA viruses ,Male ,Viral Diseases ,Coronaviruses ,Physiology ,Epidemiology ,030204 cardiovascular system & hematology ,Gastroenterology ,Biochemistry ,0302 clinical medicine ,Medical Conditions ,Mathematical and Statistical Techniques ,Patient Admission ,Interquartile range ,Medicine and Health Sciences ,030212 general & internal medicine ,Pathology and laboratory medicine ,Statistical Data ,Virus Testing ,Univariate analysis ,Multidisciplinary ,Predictive marker ,Statistics ,Medical microbiology ,Middle Aged ,Viral Load ,C-Reactive Proteins ,Prognosis ,Body Fluids ,Infectious Diseases ,Blood ,COVID-19 Nucleic Acid Testing ,Viruses ,Physical Sciences ,Regression Analysis ,RNA, Viral ,Medicine ,Female ,SARS CoV 2 ,Pathogens ,Anatomy ,Viral load ,Research Article ,medicine.medical_specialty ,SARS coronavirus ,Science ,Research and Analysis Methods ,Microbiology ,Blood Plasma ,03 medical and health sciences ,Diagnostic Medicine ,Internal medicine ,medicine ,Humans ,Statistical Methods ,Aged ,Receiver operating characteristic ,Biology and life sciences ,business.industry ,SARS-CoV-2 ,Organisms ,Viral pathogens ,Proteins ,COVID-19 ,Retrospective cohort study ,Covid 19 ,Odds ratio ,Confidence interval ,Microbial pathogens ,Nasal Mucosa ,Medical Risk Factors ,business ,Mathematics ,Biomarkers - Abstract
Background This study aimed to clarify how SARS-CoV-2 RNAemia is related to COVID-19 critical condition development and mortality in comparison with other predictive markers and scoring systems. Methods This is a retrospective cohort study conducted at Yokohama Municipal Citizen’s Hospital and National Institute of Infectious Diseases. We recruited adult patients with COVID-19 admitted between March 2020 and January 2021. We compared RNAemia with clinical status on admission including scoring systems such as the 4C Mortality, CURB-65, and A-DROP, as well as the Ct value of the nasopharyngeal PCR, in predicting COVID-19 mortality and critical condition development. Results Of the 92 recruited patients (median age, 58; interquartile range, 45–71 years), 14 (14.9%) had RNAemia. These patients had an older age (median, 68 years vs. 55.5 years; p = 0.011), higher values of lactated dehydrogenase (median, 381 U/L vs. 256.5 U/L, p < 0.001), C-reactive protein (median, 10.9 mg/dL vs. 3.8 mg/dL; p < 0.001), D-dimer (median, 2.07 μg/mL vs. 1.28 μg/mL; p = 0.015), lower values of lymphocyte (median, 802/μL vs. 1007/μL, p = 0.025) and Ct of the nasopharyngeal PCR assay (median, 20.59 vs. 25.54; p = 0.021) than those without RNAemia. Univariate analysis showed RNAemia was associated with mortality (odds ratio [OR], 18.75; 95% confidence interval [CI], 3.92–89.76; area under the receiver operating characteristic curve [AUC], 0.7851; p = 0.002) and critical condition (OR, 72.00; 95% CI, 12.98–399.29; AUC, 0.8198; p < 0.001). Plus, multivariate analysis also revealed the association of RNAemia with critical condition (adjusted OR, 125.71; 95% CI, 11.47–1377.32; p < 0.001). Conclusion On-admission SARS-CoV-2 RNAemia is a potent predictive marker of COVID-19 critical condition and mortality. The adjusted OR for critical condition was as high as 125.71.
- Published
- 2021
41. Differences in mortality and causes of death between STEMI and NSTEMI in the early and late phases after acute myocardial infarction
- Author
-
Yusuke Yoshikawa, Takeshi Kimura, Kazuya Nagao, Ko Yamamoto, Mamoru Toyofuku, Yoshihisa Nakagawa, Takeshi Morimoto, Satoru Suwa, CREDO-Kyoto Investigators, Yuki Obayashi, Eri Toda Kato, Kyohei Yamaji, Natsuhiko Ehara, Moriaki Inoko, Hiroki Sakamoto, Hiroki Watanabe, Yasuaki Takeji, Ryoji Taniguchi, Toshihiro Tamura, Yukiko Matsumura-Nakano, Hiroki Shiomi, Kenji Ando, Tomohisa Tada, Tomoya Onodera, Kenji Nakatsuma, Kazushige Kadota, Yukihito Sato, and Yutaka Furukawa
- Subjects
medicine.medical_specialty ,Critical Care and Emergency Medicine ,Cardiovascular Procedures ,Epidemiology ,medicine.medical_treatment ,Resuscitation ,Science ,Cardiology ,Myocardial Infarction ,Surgical and Invasive Medical Procedures ,Cardiovascular Medicine ,Vascular Medicine ,Primary outcome ,Medical Conditions ,Percutaneous Coronary Intervention ,Internal medicine ,Cause of Death ,medicine ,Medicine and Health Sciences ,Coronary Heart Disease ,Humans ,Myocardial infarction ,cardiovascular diseases ,Non-ST Elevated Myocardial Infarction ,Coronary Revascularization ,Heart Failure ,Multidisciplinary ,Coronary Artery Bypass Grafting ,business.industry ,Revascularization ,Confounding ,Percutaneous coronary intervention ,Cardiovascular Disease Risk ,Middle Aged ,medicine.disease ,Cardiovascular Diseases ,Heart failure ,Medical Risk Factors ,Post resuscitation ,Population study ,Medicine ,business ,Research Article - Abstract
Background The detailed causes of death in non–ST-segment–elevation myocardial infarction (NSTEMI) have not been adequately evaluated compared to those in ST-segment elevation myocardial infarction (STEMI). Methods The study population was 6,228 AMI patients who underwent percutaneous coronary intervention (STEMI: 4,625 patients and NSTEMI: 1,603 patients). The primary outcome was all-cause death. Results Within 6 months after AMI, the adjusted mortality risk was not significantly different between NSTEMI patients and STEMI patients (HR: 0.83, 95%CI: 0.67–1.03, P = 0.09). Regarding the causes of death within 6 months after AMI, mechanical complications more frequently occurred in STEMI patients than in NSTEMI patients, while proportions of post resuscitation status on arrival and heart failure were higher in in NSTEMI patients than in STEMI patients. Beyond 6 months after AMI, the adjusted mortality risk of NSTEMI relative to STEMI was not significantly different. (HR: 1.04, 95%CI: 0.90–1.20, P = 0.59). Regarding causes of death beyond 6 months after AMI, almost half of deaths were cardiovascular causes in both groups, and breakdown of causes of death was similar between NSTEMI and STEMI. Conclusion The mortality risk within and beyond 6 months after AMI were not significantly different between STEMI patients and NSTEMI patients after adjusting confounders. Deaths due to post resuscitation status and heart failure were more frequent in NSTEMI within 6 months after AMI.
- Published
- 2021
42. Teachers’ visual processing of children’s off-task behaviors in class: A comparison between teachers and student teachers
- Author
-
Kyoko Imai-Matsumura, Tsuyoshi Yamamoto, and Hirofumi Shinoda
- Subjects
Male ,Eye Movements ,Vision ,Physiology ,Visual System ,Sensory Physiology ,Social Sciences ,Task (project management) ,Visual processing ,Families ,Professional Competence ,Learning and Memory ,Sociology ,Medicine and Health Sciences ,ComputingMilieux_COMPUTERSANDEDUCATION ,Psychology ,Attention ,Children ,Class (computer programming) ,Multidisciplinary ,Schools ,Cognition ,Middle Aged ,Sensory Systems ,Professions ,Visual Perception ,Medicine ,Female ,Sensory Perception ,Anatomy ,Research Article ,Adult ,Science ,Student teacher ,Fixation, Ocular ,Education ,Young Adult ,Human Learning ,Ocular System ,Mathematics education ,Humans ,Learning ,Students ,Notice ,Cognitive Psychology ,Biology and Life Sciences ,Teachers ,Gaze ,Age Groups ,People and Places ,Cognitive Science ,Eyes ,Population Groupings ,Perception ,School Teachers ,Relevant information ,Head ,Neuroscience - Abstract
As teachers are responsible for responding instantaneously to students’ statements and actions, the progress of the class, and their teaching purpose, they need to be able to engage in responsive teaching. Teachers obtain information about students’ learning by observing them in the classroom, and subsequently make instructional decisions based on this information. Teachers need to be sensitive to student behaviors and respond accordingly, because there are students who follow the teacher’s instructions and those who do not in every classroom. Skilled teachers may distribute their gaze over the entire class and discover off-task behaviors. So how does a teacher’s visual processing and noticing ability develop? It is important to clarify this process for both experienced teachers and student teachers. Therefore, the purpose of this study was to investigate whether there is a difference in visual processing and the ability to notice off-task behaviors in class between teachers and student teachers through gaze analysis. Using an eye tracking device, 76 teachers and 147 student teachers were asked to watch a video, and gaze measurements were collected. In the video, students exhibiting off-task behaviors in class were prompted by their classroom teacher to participate in the lesson. After the video, the participants were asked if they could identify the students who had displayed off-task behaviors and whom the teachers had warned. The results showed that teachers gazed at students engaging in off-task behaviors in class more often and noticed them at a higher rate than student teachers did. These results may be attributed to differences in the experiences of visual processing of relevant information in the classroom between teachers and student teachers. Thus, the findings on teachers’ visual processing by direct measurement of gaze will be able to contribute to teachers’ development.
- Published
- 2021
43. Correction: TGF-β-induced activation of conjunctival fibroblasts is modulated by FGF-2 and substratum stiffness
- Author
-
Akiko Futakuchi, Satoshi Iraha, Yuji Takihara, Hidenobu Tanihara, Tomoyo Matsumura, Fumika Watanabe-Kitamura, Eri Takahashi, Miyuki Inoue-Mochita, Toshihiro Inoue, and Tomokazu Fujimoto
- Subjects
Multidisciplinary ,Chemistry ,Science ,TGF beta signaling pathway ,Medicine ,Fibroblast growth factor ,Cell biology - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0242626.].
- Published
- 2021
44. Threshold of increase in oxygen demand to predict mechanical ventilation use in novel coronavirus disease 2019: A retrospective cohort study incorporating restricted cubic spline regression.
- Author
-
Yamamoto, Ryo, Takemura, Ryo, Yamamoto, Asako, Matsumura, Kazuki, Kaito, Daiki, Homma, Koichiro, Wada, Michihiko, and Sasaki, Junichi
- Subjects
COVID-19 ,SARS-CoV-2 ,ARTIFICIAL respiration ,INTENSIVE care patients ,CUBIC curves ,COHORT analysis - Abstract
Background: Rapid deterioration of oxygenation occurs in novel coronavirus disease 2019 (COVID-19), and prediction of mechanical ventilation (MV) is needed for allocation of patients to intensive care unit. Since intubation is usually decided based on varying clinical conditions, such as required oxygen changes, we aimed to elucidate thresholds of increase in oxygen demand to predict MV use within 12 h. Methods: A single-center retrospective cohort study using data between January 2020 and January 2021was conducted. Data were retrieved from the hospital data warehouse. Adult patients diagnosed with COVID-19 with a positive polymerase chain reaction (PCR) who needed oxygen during admission were included. Hourly increments in oxygen demand were calculated using two consecutive oxygen values. Covariates were selected from measurements at the closest time points of oxygen data. Prediction of MV use within 12 h by required oxygen changes was evaluated with the area under the receiver operating curves (AUCs). A threshold for increased MV use risk was obtained from restricted cubic spline curves. Results: Among 66 eligible patients, 1835 oxygen data were analyzed. The AUC was 0.756 for predicting MV by oxygen demand changes, 0.888 by both amounts and changes in oxygen, and 0.933 by the model adjusted with respiratory rate, PCR quantification cycle (Ct), and days from PCR. The threshold of increments of required oxygen was identified as 0.44 L/min/h and the probability of MV use linearly increased afterward. In subgroup analyses, the threshold was lower (0.25 L/min/h) when tachypnea or frequent respiratory distress existed, whereas it was higher (1.00 L/min/h) when viral load is low (Ct ≥20 or days from PCR >7 days). Conclusions: Hourly changes in oxygen demand predicted MV use within 12 h, with a threshold of 0.44 L/min/h. This threshold was lower with an unstable respiratory condition and higher with a low viral load. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
45. Correction: TGF-β-induced activation of conjunctival fibroblasts is modulated by FGF-2 and substratum stiffness
- Author
-
Matsumura, Tomoyo, primary, Fujimoto, Tomokazu, additional, Iraha, Satoshi, additional, Futakuchi, Akiko, additional, Takihara, Yuji, additional, Watanabe-Kitamura, Fumika, additional, Takahashi, Eri, additional, Inoue-Mochita, Miyuki, additional, Tanihara, Hidenobu, additional, and Inoue, Toshihiro, additional
- Published
- 2021
- Full Text
- View/download PDF
46. Preventive effects of nitrate-rich beetroot juice supplementation on monocrotaline-induced pulmonary hypertension in rats
- Author
-
Tawa, Masashi, primary, Nagata, Rikako, additional, Sumi, Yuiko, additional, Nakagawa, Keisuke, additional, Sawano, Tatsuya, additional, Ohkita, Mamoru, additional, and Matsumura, Yasuo, additional
- Published
- 2021
- Full Text
- View/download PDF
47. TGF-β-induced activation of conjunctival fibroblasts is modulated by FGF-2 and substratum stiffness
- Author
-
Hidenobu Tanihara, Eri Takahashi, Tomokazu Fujimoto, Tomoyo Matsumura, Yuji Takihara, Toshihiro Inoue, Fumika Watanabe-Kitamura, Miyuki Inoue-Mochita, and Akiko Futakuchi
- Subjects
0301 basic medicine ,Cell signaling ,Fibroblast Growth Factor ,Physiology ,Signal transduction ,Fibroblast growth factor ,Biochemistry ,Stiffness ,0302 clinical medicine ,Endocrinology ,Animal Cells ,Gene expression ,Medicine and Health Sciences ,Myofibroblasts ,Connective Tissue Cells ,Transdifferentiation ,Multidisciplinary ,medicine.diagnostic_test ,biology ,Chemistry ,Physics ,Signaling cascades ,Classical Mechanics ,Connective Tissue ,Cell Processes ,Physical Sciences ,Medicine ,Mechanical Stress ,Fibroblast Growth Factor 2 ,Cellular Types ,Anatomy ,Conjunctiva ,Myofibroblast ,Research Article ,Cell biology ,Cell Physiology ,Science ,Materials Science ,Material Properties ,Collagen Type I ,Cell Line ,Transforming Growth Factor beta2 ,03 medical and health sciences ,Western blot ,Growth Factors ,medicine ,Humans ,Mechanical Properties ,Adaptor Proteins, Signal Transducing ,Cell Proliferation ,Endocrine Physiology ,Biology and life sciences ,Cell growth ,Correction ,Proteins ,YAP-Signaling Proteins ,Fibroblasts ,Molecular biology ,Actins ,Fibronectin ,030104 developmental biology ,Biological Tissue ,Gene Expression Regulation ,TGF-beta signaling cascade ,Cell Transdifferentiation ,030221 ophthalmology & optometry ,biology.protein ,Collagens ,Transcription Factors ,Transforming growth factor - Abstract
Purpose This study aimed to investigate the effects of substratum stiffness on the sensitivity of human conjunctival fibroblasts to transforming growth factor (TGF)-β, and to explore the molecular mechanism of action. Methods Human conjunctival fibroblasts were cultured on collagen-coated plastic or silicone plates. The stiffness of the silicone plates was 0.2 or 64 kPa. Cells were treated by 2.5 ng/mL TGF-β2 with or without fibroblast growth factor (FGF)-2 (0–100 ng/mL) for 24 h or 48 h. The protein expression levels were determined by Western blot analysis. Cell proliferation was assessed using the WST-8 assay. Results FGF-2 suppressed the TGF-β-induced expression of α-smooth muscle actin (SMA) and collagen type I (Col I), but not fibronectin (FN). Both FGF-2 and TGF-β2 increased cell proliferation without an additive effect. The induction of α-SMA by TGF-β2 was decreased on the soft substratum, without any change in the expression level or subcellular location of Yes-associated protein/transcriptional coactivator with PDZ-binding motif (YAP/TAZ). FGF-2 suppressed TGF-β-induced α-SMA expression even on the soft substratum. Conclusions FGF-2 treatment and a soft substratum suppressed TGF-β-induced transdifferentiation of conjunctival fibroblasts into myofibroblasts. FGF-2 attenuated the TGF-β-induced expression of α-SMA, even on a soft substratum.
- Published
- 2020
48. Disruption of CRTC1 and CRTC2 in Sim1 cells strongly increases high-fat diet intake in female mice but has a modest impact on male mice
- Author
-
Jin Tanaka, Fuka Ishikawa, Tomoki Jinno, Motoki Miyakita, Haruka Miyamori, Tsutomu Sasaki, Takumi Yokokawa, Tsuyoshi Goto, Kazuo Inoue, and Shigenobu Matsumura
- Subjects
Male ,Physiology ,Science ,Hypothalamus ,Bioengineering ,Hyperphagia ,Bioenergetics ,Diet, High-Fat ,Weight Gain ,Biochemistry ,Mice ,Basic Helix-Loop-Helix Transcription Factors ,Medicine and Health Sciences ,Animals ,Obesity ,Nutrition ,Mice, Knockout ,Multidisciplinary ,Genetically Modified Animals ,Genetically Modified Organisms ,Body Weight ,Biology and Life Sciences ,Brain ,Feeding Behavior ,Diet ,Mice, Inbred C57BL ,Repressor Proteins ,Physiological Parameters ,Food ,Medicine ,Engineering and Technology ,Female ,Anatomy ,Energy Metabolism ,Genetic Engineering ,Transcription Factors ,Research Article ,Biotechnology - Abstract
cAMP responsive element binding protein (CREB)-regulated transcription coactivators (CRTCs) regulate gene transcription in response to an increase in intracellular cAMP or Ca2+ levels. To date, three isoforms of CRTC have been identified in mammals. All CRTCs are widely expressed in various regions of the brain. Numerous studies have shown the importance of CREB and CRTC in energy homeostasis. In the brain, the paraventricular nucleus of the hypothalamus (PVH) plays a critical role in energy metabolism, and CRTC1 and CRTC2 are highly expressed in PVH neuronal cells. The single-minded homolog 1 gene (Sim1) is densely expressed in PVH neurons and in some areas of the amygdala neurons. To determine the role of CRTCs in PVH on energy metabolism, we generated mice that lacked CRTC1 and CRTC2 in Sim1 cells using Sim-1 cre mice. We found that Sim1 cell-specific CRTC1 and CRTC2 double-knockout mice were sensitive to high-fat diet (HFD)-induced obesity. Sim1 cell-specific CRTC1 and CRTC2 double knockout mice showed hyperphagia specifically for the HFD, but not for the normal chow diet, increased fat mass, and no change in energy expenditure. Interestingly, these phenotypes were stronger in female mice than in male mice, and a weak phenotype was observed in the normal chow diet. The lack of CRTC1 and CRTC2 in Sim1 cells changed the mRNA levels of some neuropeptides that regulate energy metabolism in female mice fed an HFD. Taken together, our findings suggest that CRTCs in Sim1 cells regulate gene expression and suppress excessive fat intake, especially in female mice.
- Published
- 2022
49. Amplitude of circadian rhythms becomes weaken in the north, but there is no cline in the period of rhythm in a beetle
- Author
-
Abe, Masato S., primary, Matsumura, Kentarou, additional, Yoshii, Taishi, additional, and Miyatake, Takahisa, additional
- Published
- 2021
- Full Text
- View/download PDF
50. Impact of brain natriuretic peptide reduction on the worsening renal function in patients with acute heart failure
- Author
-
Masayoshi Yamamoto, Satoshi Suzuki, Shunsuke Kuroda, Kenji Yoshioka, Akihiko Matsumura, Takahiro Okumura, Keisuke Kida, Shogo Oishi, Yuya Matsue, Takeshi Kitai, Tetsuo Sasano, Nobuyuki Kagiyama, Eiichi Akiyama, Akira Mizukami, and Tetsuo Yamaguchi
- Subjects
Male ,Peptide Hormones ,030204 cardiovascular system & hematology ,Kidney ,Biochemistry ,Brain Natriuretic Peptide ,chemistry.chemical_compound ,Mathematical and Statistical Techniques ,0302 clinical medicine ,Natriuretic Peptide, Brain ,Medicine and Health Sciences ,Natriuretic peptide ,030212 general & internal medicine ,Diuretics ,Aged, 80 and over ,Multidisciplinary ,Ejection fraction ,Statistics ,Hazard ratio ,Drugs ,Prognosis ,Brain natriuretic peptide ,Creatinine ,Physical Sciences ,Acute Disease ,Cardiology ,Regression Analysis ,Medicine ,Female ,Anatomy ,hormones, hormone substitutes, and hormone antagonists ,Research Article ,medicine.medical_specialty ,medicine.drug_class ,Science ,Renal function ,Research and Analysis Methods ,03 medical and health sciences ,Natriuretic Peptide ,Diagnostic Medicine ,Internal medicine ,medicine ,Humans ,cardiovascular diseases ,Statistical Methods ,Aged ,Heart Failure ,Pharmacology ,Proportional hazards model ,business.industry ,Biology and Life Sciences ,Renal System ,medicine.disease ,Hormones ,chemistry ,Heart failure ,business ,human activities ,Biomarkers ,Mathematics ,Ejection Fraction - Abstract
AimsThe prognostic impact of worsening renal function (WRF) in patients with acute heart failure (AHF) remains under debate. Successful decongestion might offset the negative impact of WRF, but little is known about indicators of successful decongestion in the very acute phase of AHF. We hypothesized that decongestion as evaluated by the percent reduction in brain natriuretic peptide (BNP) could identify relevant prognostic implications of WRF in the very acute phase of AHF.Methods and resultsData on 907 consecutive hospitalized patients with AHF in the REALITY-AHF study (age: 78±12 years; 55.1% male) were analyzed. Creatinine and BNP were measured at baseline and 48 hours from admission. WRF was defined as an increase in creatinine >0.3 mg at 48 hours from admission. The primary endpoint was 1-year all-cause mortality. Patients were divided into four groups according to the presence/absence of WRF and a BNP reduction higher/lower than the median: no-WRF/higher-BNP-reduction (n = 390), no-WRF/lower-BNP-reduction (n = 397), WRF/higher-BNP-reduction (n = 63), and WRF/lower-BNP-reduction groups (n = 57). Kaplan-Meier curve analysis showed that the WRF/lower-BNP-reduction group had a worse prognosis than the other groups. In a Cox regression analysis, only the WRF/lower-BNP-reduction group had higher mortality compared to the no-WRF/higher-BNP-reduction group (hazard ratio: 3.34, pConclusionIn the very acute phase of AHF, BNP reduction may aid in identifying relevant prognostic significance of WRF.
- Published
- 2020
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.