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2. Detection of Streptococcus pneumoniae and Haemophilus influenzae type B by real-time PCR from dried blood spot samples among children with pneumonia: a useful approach for developing countries.

Author

Laura Selva, Rachid Benmessaoud, Miguel Lanaspa, Imane Jroundi, Cinta Moraleda, Sozinho Acacio, Melania Iñigo, Alien Bastiani, Manuel Monsonis, Roman Pallares, Quique Bassat, and Carmen Muñoz-Almagro

Subjects
Medicine, Science
Abstract

BACKGROUND:Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco. METHODS:Ply and wzg genes of S. pneumoniae and bexA gene of Hib, were used as targets of Real-Time PCR. 329 DBS samples of children hospitalized with clinical diagnosis of pneumonia were tested. RESULTS:Real-Time PCR in DBS allowed for a significant increase in microbiological diagnosis of S. pneumoniae and Hib. When performing blood bacterial culture, only ten isolates of S. pneumoniae and none of Hib were detected (3·0% positivity rate, IC95% 1·4-5·5%). Real-Time PCR from DBS samples increased the detection yield by 4x fold, as 30 S. pneumoniae and 11 Hib cases were detected (12·4% positivity rate, IC95% 9·0-16·5%; P

Published
2013
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3. Detection of Streptococcus pneumoniae and Haemophilus influenzae Type B by Real-Time PCR from Dried Blood Spot Samples among Children with Pneumonia: A Useful Approach for Developing Countries

Author

Sozinho Acácio, Carmen Muñoz-Almagro, Imane Jroundi, Miguel A. Lanaspa, Quique Bassat, Melania Iñigo, Roman Pallares, Laura Selva, Alien Bastiani, Manuel Monsonis, Cinta Moraleda, Rachid Benmessaoud, and Universitat de Barcelona

Subjects
Streptococcus pneumonia, Microbiological culture, Haemophilus influenzae type, lcsh:Medicine, Pneumònia, medicine.disease_cause, Haemophilus influenzae, Microbiology, law.invention, law, Reacció en cadena de la polimerasa, Streptococcus pneumoniae, medicine, lcsh:Science, Children, Polymerase chain reaction, Multidisciplinary, business.industry, Pneumococs, lcsh:R, Pneumonia, medicine.disease, Virology, Dried blood spot, respiratory tract diseases, Real-time polymerase chain reaction, Pathogenic bacteria, Bacteris patògens, lcsh:Q, business, Infants, therapeutics, Research Article
Abstract

Correction https://doi.org/10.1371/journal.pone.0147678, Background: Dried blood spot (DBS) is a reliable blood collection method for storing samples at room temperature and easily transporting them. We have previously validated a Real-Time PCR for detection of Streptococcus pneumoniae in DBS. The objective of this study was to apply this methodology for the diagnosis of S. pneumoniae and Haemophilus influenzae b (Hib) in DBS samples of children with pneumonia admitted to two hospitals in Mozambique and Morocco. Methods: Ply and wzg genes of S. pneumoniae and bexA gene of Hib, were used as targets of Real-Time PCR. 329 DBS samples of children hospitalized with clinical diagnosis of pneumonia were tested. Results: Real-Time PCR in DBS allowed for a significant increase in microbiological diagnosis of S. pneumoniae and Hib. When performing blood bacterial culture, only ten isolates of S. pneumoniae and none of Hib were detected (3·0% positivity rate, IC95% 1·4-5·5%). Real-Time PCR from DBS samples increased the detection yield by 4x fold, as 30 S. pneumoniae and 11 Hib cases were detected (12·4% positivity rate, IC95% 9·0-16·5%; P

Published
2013

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