1. Evaluation of antibody responses to panels of M. tuberculosis antigens as a screening tool for active tuberculosis in Uganda.
- Author
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Shete, Priya B, Ravindran, Resmi, Chang, Emily, Worodria, William, Chaisson, Lelia H, Andama, Alfred, Davis, J Lucian, Luciw, Paul A, Huang, Laurence, Khan, Imran H, and Cattamanchi, Adithya
- Subjects
Humans ,Tuberculosis ,Recombinant Proteins ,Antibodies ,Bacterial ,Antigens ,Bacterial ,Immunoassay ,Sensitivity and Specificity ,Algorithms ,Adult ,Middle Aged ,Uganda ,Female ,Male ,Antibodies ,Bacterial ,Antigens ,General Science & Technology - Abstract
BackgroundImproved systematic screening of high-risk groups is a key component of the tuberculosis (TB) elimination strategy endorsed by the World Health Organization (WHO). We used a multiplex microbead immunoassay to measure antibody responses to 28 M. tuberculosis (M.tb) antigens, and assessed whether combinations of antibody responses achieve accuracy thresholds required for a TB screening test.MethodsA random selection of plasma samples obtained from consecutive HIV-negative adults who were admitted to Mulago Hospital in Kampala, Uganda with cough ≥2 weeks' but 90%, specificity remained close to 70% with as few as 3 antigens included in the panels.ConclusionsMeasuring antibody responses to combinations of antigens could facilitate TB screening and should be further evaluated in populations being targeted for systematic screening.
- Published
- 2017