Search

Your search keyword '"Libo Zhu"' showing total 4 results
Start Over Author "Libo Zhu" Journal plos one
4 results on '"Libo Zhu"'

1. P2X3 receptor involvement in endometriosis pain via ERK signaling pathway.

Author

Shaojie Ding, Libo Zhu, Yonghong Tian, Tianhong Zhu, Xiufeng Huang, and Xinmei Zhang

Subjects
Medicine, Science
Abstract

The purinergic receptor P2X ligand-gated ion channel 3 (P2X3) is crucially involved in peripheral nociceptive processes of somatic and visceral pain. Endometriosis pain is considered as a kind of inflammatory and neuropathic pain. However, whether P2X3 is involved in endometriosis pain has not been reported up to date. Here, we aimed to determine whether P2X3 expression in endometriotic lesions is involved in endometriosis pain, which is regulated by inflammatory mediators through extracellular regulated protein kinases (ERK) signalling pathway. We found that P2X3 expressions in endometriosis endometrium and endometriotic lesions were both significantly higher as compared with control endometrium (P

Published
2017
Full Text
View/download PDF

2. Pigment epithelium derived factor inhibits the growth of human endometrial implants in nude mice and of ovarian endometriotic stromal cells in vitro.

Author

Yanmei Sun, Xuan Che, Libo Zhu, Mengdan Zhao, Guofang Fu, Xiufeng Huang, Hong Xu, Fuqiang Hu, and Xinmei Zhang

Subjects
Medicine, Science
Abstract

Angiogenesis is a prerequisite for the formation and development of endometriosis. Pigment epithelium derived factor (PEDF) is a natural inhibitor of angiogenesis. We previously demonstrated a reduction of PEDF in the peritoneal fluid, serum and endometriotic lesions from women with endometriosis compared with women without endometriosis. Here, we aim to investigate the inhibitory effect of PEDF on human endometriotic cells in vivo and in vitro. We found that PEDF markedly inhibited the growth of human endometrial implants in nude mice and of ovarian endometriotic stromal cells in vitro by up-regulating PEDF expression and down-regulating vascular endothelial growth factor (VEGF) expression. Moreover, apoptotic index was significantly increased in endometriotic lesions in vivo and endometriotic stromal cells in vitro when treated with PEDF. In mice treated with PEDF, decreased microvessel density labeled by Von Willebrand factor but not by α-Smooth Muscle Actin was observed in endometriotic lesions. And it showed no increase in PEDF expression of the ovary and uterus tissues. These findings suggest that PEDF gene therapy may be a new treatment for endometriosis.

Published
2012
Full Text
View/download PDF

3. P2X3 receptor involvement in endometriosis pain via ERK signaling pathway

Author

Libo Zhu, Yonghong Tian, Shaojie Ding, Xiufeng Huang, Xinmei Zhang, and Tianhong Zhu

Subjects
MAPK/ERK pathway, Sensory Receptors, Physiology, Sensory Physiology, Protein Expression, Endometriosis, lcsh:Medicine, Social Sciences, Pathology and Laboratory Medicine, Endometrium, 0302 clinical medicine, Nerve Fibers, Animal Cells, Medicine and Health Sciences, Psychology, lcsh:Science, Cyclic AMP Response Element-Binding Protein, Immune Response, Connective Tissue Cells, Peak Values, Neurons, Mitogen-Activated Protein Kinase 1, 030219 obstetrics & reproductive medicine, Multidisciplinary, Mitogen-Activated Protein Kinase 3, Kinase, Purinergic receptor, Visceral Pain, Sensory Systems, Somatosensory System, Connective Tissue, Neuropathic pain, Engineering and Technology, Sensory Perception, Female, Signal transduction, medicine.symptom, Anatomy, Cellular Types, Research Article, Signal Transduction, Adult, medicine.medical_specialty, MAP Kinase Signaling System, Calcitonin Gene-Related Peptide, Immunology, Calcitonin gene-related peptide, Research and Analysis Methods, 03 medical and health sciences, Signs and Symptoms, Diagnostic Medicine, Internal medicine, medicine, Gene Expression and Vector Techniques, Humans, Molecular Biology Techniques, Molecular Biology, Inflammation, Molecular Biology Assays and Analysis Techniques, business.industry, lcsh:R, Uterus, Reproductive System, Biology and Life Sciences, Pain Sensation, Visceral pain, Cell Biology, medicine.disease, body regions, Endocrinology, Biological Tissue, Cellular Neuroscience, Case-Control Studies, Signal Processing, lcsh:Q, Stromal Cells, business, 030217 neurology & neurosurgery, Receptors, Purinergic P2X3, Neuroscience
Abstract

The purinergic receptor P2X ligand-gated ion channel 3 (P2X3) is crucially involved in peripheral nociceptive processes of somatic and visceral pain. Endometriosis pain is considered as a kind of inflammatory and neuropathic pain. However, whether P2X3 is involved in endometriosis pain has not been reported up to date. Here, we aimed to determine whether P2X3 expression in endometriotic lesions is involved in endometriosis pain, which is regulated by inflammatory mediators through extracellular regulated protein kinases (ERK) signalling pathway. We found that P2X3 expressions in endometriosis endometrium and endometriotic lesions were both significantly higher as compared with control endometrium (P

Published
2017

4. Pigment Epithelium Derived Factor Inhibits the Growth of Human Endometrial Implants in Nude Mice and of Ovarian Endometriotic Stromal Cells In Vitro

Author

Xuan Che, Xinmei Zhang, Guofang Fu, Fuqiang Hu, Hong Xu, Yanmei Sun, Libo Zhu, Xiufeng Huang, and Meng-Dan Zhao

Subjects
Vascular Endothelial Growth Factor A, Anatomy and Physiology, Mouse, Angiogenesis, lcsh:Medicine, Apoptosis, Endometrium, Neovascularization, Mice, chemistry.chemical_compound, Morphogenesis, lcsh:Science, Multidisciplinary, Neovascularization, Pathologic, Obstetrics and Gynecology, Animal Models, Vascular endothelial growth factor, Female Genital Diseases, medicine.anatomical_structure, Medicine, Female, medicine.symptom, Genital Anatomy, Research Article, Adult, medicine.medical_specialty, Stromal cell, Transplantation, Heterologous, Endometriosis, Mice, Nude, Ovary, Cell Migration, Model Organisms, PEDF, Internal medicine, von Willebrand Factor, medicine, Animals, Humans, Nerve Growth Factors, Eye Proteins, Biology, Serpins, lcsh:R, Reproductive System, Actins, Transplantation, Endocrinology, Gene Expression Regulation, chemistry, Microvessels, Cancer research, lcsh:Q, Stromal Cells, Menstrual Abnormalities, Developmental Biology
Abstract

Angiogenesis is a prerequisite for the formation and development of endometriosis. Pigment epithelium derived factor (PEDF) is a natural inhibitor of angiogenesis. We previously demonstrated a reduction of PEDF in the peritoneal fluid, serum and endometriotic lesions from women with endometriosis compared with women without endometriosis. Here, we aim to investigate the inhibitory effect of PEDF on human endometriotic cells in vivo and in vitro. We found that PEDF markedly inhibited the growth of human endometrial implants in nude mice and of ovarian endometriotic stromal cells in vitro by up-regulating PEDF expression and down-regulating vascular endothelial growth factor (VEGF) expression. Moreover, apoptotic index was significantly increased in endometriotic lesions in vivo and endometriotic stromal cells in vitro when treated with PEDF. In mice treated with PEDF, decreased microvessel density labeled by Von Willebrand factor but not by α-Smooth Muscle Actin was observed in endometriotic lesions. And it showed no increase in PEDF expression of the ovary and uterus tissues. These findings suggest that PEDF gene therapy may be a new treatment for endometriosis.

Published
2012

Catalog

Books, media, physical & digital resources
See catalog results