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2. Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression.

Author

Xiu-Jing Yue, Lei-Bo Xu, Man-Sheng Zhu, Rui Zhang, and Chao Liu

Subjects
Medicine, Science
Abstract

AIMS: It has been shown that nerve growth factor-β (NGF-β) promoted the initiation and progression of many tumors, and we have previously demonstrated that the expression of NGF-β was associated with tumor stage, nerve infiltration and lymph node metastasis in human hilar cholangiocarcinoma. However, whether NGF-β promotes tumor progression in human cholangiocarcinoma requires further investigation. Therefore, we aimed to determine the effects of NGF-β on the progression of human cholangiocarcinoma. METHODS: Human cholangiocarcinoma QBC939 stable cell lines with over-expressed or silenced NGF-β genes were generated with pEGFP-N1-NGF-β and pGPU6/GFP/Neo-NGF-β-shRNA recombinant plasmids. Cell proliferation assay, colony formation assay, cell cycle analysis, apoptosis assay and tumorigenicity assay were performed to evaluate the role of NGF-β in the progression of human cholangiocarcinoma. In addition, human lymphatic endothelial cells were co-cultured with QBC939 culture supernatants, and the cell proliferation and migration abilities of the lymphatic endothelial cells were evaluated. RESULTS: Forced expression of NGF-β in QBC939 cell lines promoted proliferation, colony formation and tumorigenicity in these cells and inhibited the apoptosis. However, down-regulation of NGF-β inhibited proliferation, colony formation and tumorigenicity, and increased the apoptotic rate of QBC939 cells. In addition, the NGF-β gain-of-function induced a high expression of vascular endothelial growth factor C and enhanced the proliferation and migration of lymphatic endothelial cells, while NGF-β loss-of-function showed opposite effects. CONCLUSIONS: We concluded that NGF-β promoted tumor progression in human cholangiocarcinoma QBC939 cells. Our results provided a new concept to understand the role of NGF-β in cholangiocarcinoma progression, and might provide important information for the development of new targeted therapies in human cholangiocarcinoma.

Published
2013
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5. Correction: AAV-Mediated Cone Rescue in a Naturally Occurring Mouse Model of CNGA3-Achromatopsia

Author

Pang, Ji-jing, primary, Deng, Wen-Tao, additional, Dai, Xufeng, additional, Lei, Bo, additional, Everhart, Drew, additional, Umino, Yumiko, additional, Li, Jie, additional, Zhang, Keqing, additional, Mao, Song, additional, Boye, Sanford L., additional, Liu, Li, additional, Chiodo, Vince A., additional, Liu, Xuan, additional, Shi, Wei, additional, Tao, Ye, additional, Chang, Bo, additional, and Hauswirth, William W., additional

Published
2014
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7. Over-Expression of Nerve Growth Factor-β in Human Cholangiocarcinoma QBC939 Cells Promote Tumor Progression

Author

Man-sheng Zhu, Lei-Bo Xu, Chao Liu, Rui Zhang, and Xiu-jing Yue

Subjects
Pathology, Anatomy and Physiology, Vascular Endothelial Growth Factor C, Cancer Treatment, lcsh:Medicine, Gene Expression, Apoptosis, Biochemistry, Metastasis, Cholangiocarcinoma, Mice, Cell Movement, Nerve Growth Factor, Molecular Cell Biology, Basic Cancer Research, Gene Order, lcsh:Science, Multidisciplinary, Cell Cycle, Neurochemistry, Cell cycle, Clinical Laboratory Sciences, Tumor Burden, Gene Expression Regulation, Neoplastic, Cell Transformation, Neoplastic, Oncology, Vascular endothelial growth factor C, Disease Progression, Medicine, Heterografts, Female, Neurochemicals, Research Article, Cell Physiology, medicine.medical_specialty, Cytokine Therapy, Biology, Diagnostic Medicine, Cell Line, Tumor, Gastrointestinal Tumors, medicine, Animals, Humans, Gene Silencing, Cell Proliferation, Cell growth, lcsh:R, Cancers and Neoplasms, medicine.disease, Nerve growth factor, Cell culture, Tumor progression, Cancer research, lcsh:Q, Neuroscience
Abstract

Aims It has been shown that nerve growth factor-β (NGF-β) promoted the initiation and progression of many tumors, and we have previously demonstrated that the expression of NGF-β was associated with tumor stage, nerve infiltration and lymph node metastasis in human hilar cholangiocarcinoma. However, whether NGF-β promotes tumor progression in human cholangiocarcinoma requires further investigation. Therefore, we aimed to determine the effects of NGF-β on the progression of human cholangiocarcinoma. Methods Human cholangiocarcinoma QBC939 stable cell lines with over-expressed or silenced NGF-β genes were generated with pEGFP-N1-NGF-β and pGPU6/GFP/Neo-NGF-β-shRNA recombinant plasmids. Cell proliferation assay, colony formation assay, cell cycle analysis, apoptosis assay and tumorigenicity assay were performed to evaluate the role of NGF-β in the progression of human cholangiocarcinoma. In addition, human lymphatic endothelial cells were co-cultured with QBC939 culture supernatants, and the cell proliferation and migration abilities of the lymphatic endothelial cells were evaluated. Results Forced expression of NGF-β in QBC939 cell lines promoted proliferation, colony formation and tumorigenicity in these cells and inhibited the apoptosis. However, down-regulation of NGF-β inhibited proliferation, colony formation and tumorigenicity, and increased the apoptotic rate of QBC939 cells. In addition, the NGF-β gain-of-function induced a high expression of vascular endothelial growth factor C and enhanced the proliferation and migration of lymphatic endothelial cells, while NGF-β loss-of-function showed opposite effects. Conclusions We concluded that NGF-β promoted tumor progression in human cholangiocarcinoma QBC939 cells. Our results provided a new concept to understand the role of NGF-β in cholangiocarcinoma progression, and might provide important information for the development of new targeted therapies in human cholangiocarcinoma.

Published
2013

11. AAV-Mediated Cone Rescue in a Naturally Occurring Mouse Model of CNGA3-Achromatopsia

Author

Pang, Ji-jing, primary, Deng, Wen-Tao, additional, Dai, Xufeng, additional, Lei, Bo, additional, Everhart, Drew, additional, Umino, Yumiko, additional, Li, Jie, additional, Zhang, Keqing, additional, Mao, Song, additional, Boye, Sanford L., additional, Liu, Li, additional, Chiodo, Vince A., additional, Liu, Xuan, additional, Shi, Wei, additional, Tao, Ye, additional, Chang, Bo, additional, and Hauswirth, William W., additional

Published
2012
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