1. Effect of hepatitis C infection on HIV-induced apoptosis
- Author
-
Karen V. Kibler, Andrzej Horban, Marek Radkowski, G Stanczak, Tomasz Laskus, Marcin K. Chmielewski, Debra Adair, and Jeffrey Wilkinson
- Subjects
Adult ,CD4-Positive T-Lymphocytes ,Male ,Hepatitis C virus ,Human immunodeficiency virus (HIV) ,lcsh:Medicine ,Apoptosis ,HIV Infections ,CD8-Positive T-Lymphocytes ,medicine.disease_cause ,Cell Line ,Liver disease ,medicine ,Cytotoxic T cell ,Humans ,lcsh:Science ,Multidisciplinary ,business.industry ,Coinfection ,lcsh:R ,virus diseases ,Hepatitis C ,medicine.disease ,Virology ,digestive system diseases ,Cell culture ,Immunology ,Female ,lcsh:Q ,business ,Research Article - Abstract
BACKGROUND: Hepatitis C virus (HCV) coinfection was reported to negatively affect HIV disease and HIV infection has a deleterious effect on HCV-related liver disease. However, despite common occurrence of HCV/HIV coinfection little is known about the mechanisms of interactions between the two viruses. METHODS: We studied CD4+ and CD8+ T cell and CD19+ B cell apoptosis in 104 HIV-positive patients (56 were also HCV-positive) and in 22 HCV/HIV-coinfected patients treated for chronic hepatitis C with pegylated interferon and ribavirin. We also analyzed HCV/HIV coinfection in a Daudi B-cell line expressing CD4 and susceptible to both HCV and HIV infection. Apoptosis was measured by AnnexinV staining. RESULTS: HCV/HIV coinfected patients had lower CD4+ and CD8+ T cell apoptosis and higher CD19+ B cell apoptosis than those with HIV monoinfection. Furthermore, anti-HCV treatment of HCV/HIV coinfected patients was followed by an increase of CD4+ and CD8+ T cell apoptosis and a decrease of CD19+ B cell apoptosis. In the Daudi CD4+ cell line, presence of HCV infection facilitated HIV replication, however, decreased the rate of HIV-related cell death. CONCLUSION: In HCV/HIV coinfected patients T-cells were found to be destroyed at a slower rate than in HIV monoinfected patients. These results suggest that HCV is a molecular-level determinant in HIV disease.
- Published
- 2013