1. Full-field optical coherence tomography for the diagnosis of giant cell arteritis
- Author
-
Emilie Benoit, Sylvain Audia, Pierre-Henry Gabrielle, Maxime Samson, Hélène Greigert, Olivier Clément, Laurent Martin, Bernard Bonnotte, Jean-Marie Chassot, Daniel Balvay, Claude Boccara, Catherine Creuzot-Garcher, Bertrand Tavitian, Thomas Maldiney, Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut National de la Santé et de la Recherche Médicale (INSERM), LLTech SAS Paris, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Langevin - Ondes et Images (UMR7587) (IL), Sorbonne Université (SU)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), Paris-Centre de Recherche Cardiovasculaire (PARCC (UMR_S 970/ U970)), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Paris (UP), Julien, Sabine, Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité)
- Subjects
Male ,genetic structures ,Biopsy ,Pathology and Laboratory Medicine ,01 natural sciences ,Diagnostic Radiology ,Cohort Studies ,0302 clinical medicine ,Medicine and Health Sciences ,Prospective Studies ,skin and connective tissue diseases ,Tomography ,Immune Response ,Aged, 80 and over ,Microscopy ,Multidisciplinary ,medicine.diagnostic_test ,Radiology and Imaging ,Light Microscopy ,Software Engineering ,Arteries ,Temporal artery biopsy ,Middle Aged ,Temporal Arteries ,medicine.anatomical_structure ,[SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs ,cardiovascular system ,Medicine ,Engineering and Technology ,Female ,Temporal artery ,Radiology ,Anatomy ,Tunica Media ,Tomography, Optical Coherence ,Research Article ,Computer and Information Sciences ,medicine.medical_specialty ,Histology ,Imaging Techniques ,Science ,Immunology ,Giant Cell Arteritis ,Surgical and Invasive Medical Procedures ,Research and Analysis Methods ,Computer Software ,010309 optics ,Interference Microscopy ,03 medical and health sciences ,Signs and Symptoms ,Optical coherence tomography ,Diagnostic Medicine ,0103 physical sciences ,medicine ,Humans ,[SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs ,Aged ,Inflammation ,030203 arthritis & rheumatology ,business.industry ,Biology and Life Sciences ,Full field ,medicine.disease ,Internal elastic lamina ,Giant cell arteritis ,Anatomical Pathology ,Surgical Pathology ,Case-Control Studies ,Vasa vasorum ,Reticular connective tissue ,Cardiovascular Anatomy ,Blood Vessels ,Clinical Medicine ,Tunica Intima ,business - Abstract
Histopathological examination of temporal artery biopsy (TAB) remains the gold standard for the diagnosis of giant cell arteritis (GCA) but is associated with essential limitations that emphasize the need for an upgraded pathological process. This study pioneered the use of full-field optical coherence tomography (FF-OCT) for rapid and automated on-site pathological diagnosis of GCA. Sixteen TABs (12 negative and 4 positive for GCA) were selected according to major histopathological criteria of GCA following hematoxylin-eosin-saffron-staining for subsequent acquisition with FF-OCT to compare structural modifications of the artery cell wall and thickness of each tunica. Gabor filtering of FF-OCT images was then used to compute TAB orientation maps and validate a potential automated analysis of TAB sections. FF-OCT allowed both qualitative and quantitative visualization of the main structures of the temporal artery wall, from the internal elastic lamina to the vasa vasorum and red blood cells, unveiling a significant correlation with conventional histology. FF-OCT imaging of GCA TABs revealed destruction of the media with distinct remodeling of the whole arterial wall into a denser reticular fibrous neo-intima, which is distinctive of GCA pathogenesis and accessible through automated Gabor filtering. Rapid on-site FF-OCT TAB acquisition makes it possible to identify some characteristic pathological lesions of GCA within a few minutes, paving the way for potential machine intelligence-based or even non-invasive diagnosis of GCA.
- Published
- 2020