233 results on '"Isabelle M"'
Search Results
2. Correction: Correlation between hospitalized patients' demographics, symptoms, comorbidities, and COVID-19 pandemic in Bahia, Brazil.
- Author
-
Márcio C F Macedo, Isabelle M Pinheiro, Caio J L Carvalho, Hilda C J R Fraga, Isaac P C Araujo, Simone S Montes, Otávio A C Araujo, Lucas A Alves, Hugo Saba, Márcio L V Araújo, Ivonete T L Queiroz, Romilson L Sampaio, Márcia S P L Souza, Ana Claudia F N da Silva, and Antonio C S Souza
- Subjects
Medicine ,Science - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0243966.].
- Published
- 2021
- Full Text
- View/download PDF
3. Identifying causative mechanisms linking early-life stress to psycho-cardio-metabolic multi-morbidity: The EarlyCause project.
- Author
-
Nicole Mariani, Alessandra Borsini, Charlotte A M Cecil, Janine F Felix, Sylvain Sebert, Annamaria Cattaneo, Esther Walton, Yuri Milaneschi, Guy Cochrane, Clara Amid, Jeena Rajan, Juliette Giacobbe, Yolanda Sanz, Ana Agustí, Tania Sorg, Yann Herault, Jouko Miettunen, Priyanka Parmar, Nadia Cattane, Vincent Jaddoe, Jyrki Lötjönen, Carme Buisan, Miguel A González Ballester, Gemma Piella, Josep L Gelpi, Femke Lamers, Brenda W J H Penninx, Henning Tiemeier, Malte von Tottleben, Rainer Thiel, Katharina F Heil, Marjo-Riitta Järvelin, Carmine Pariante, Isabelle M Mansuy, and Karim Lekadir
- Subjects
Medicine ,Science - Abstract
IntroductionDepression, cardiovascular diseases and diabetes are among the major non-communicable diseases, leading to significant disability and mortality worldwide. These diseases may share environmental and genetic determinants associated with multimorbid patterns. Stressful early-life events are among the primary factors associated with the development of mental and physical diseases. However, possible causative mechanisms linking early life stress (ELS) with psycho-cardio-metabolic (PCM) multi-morbidity are not well understood. This prevents a full understanding of causal pathways towards the shared risk of these diseases and the development of coordinated preventive and therapeutic interventions.Methods and analysisThis paper describes the study protocol for EarlyCause, a large-scale and inter-disciplinary research project funded by the European Union's Horizon 2020 research and innovation programme. The project takes advantage of human longitudinal birth cohort data, animal studies and cellular models to test the hypothesis of shared mechanisms and molecular pathways by which ELS shapes an individual's physical and mental health in adulthood. The study will research in detail how ELS converts into biological signals embedded simultaneously or sequentially in the brain, the cardiovascular and metabolic systems. The research will mainly focus on four biological processes including possible alterations of the epigenome, neuroendocrine system, inflammatome, and the gut microbiome. Life-course models will integrate the role of modifying factors as sex, socioeconomics, and lifestyle with the goal to better identify groups at risk as well as inform promising strategies to reverse the possible mechanisms and/or reduce the impact of ELS on multi-morbidity development in high-risk individuals. These strategies will help better manage the impact of multi-morbidity on human health and the associated risk.
- Published
- 2021
- Full Text
- View/download PDF
4. Correlation between hospitalized patients' demographics, symptoms, comorbidities, and COVID-19 pandemic in Bahia, Brazil.
- Author
-
Márcio C F Macedo, Isabelle M Pinheiro, Caio J L Carvalho, Hilda C J R Fraga, Isaac P C Araujo, Simone S Montes, Otávio A C Araujo, Lucas A Alves, Hugo Saba, Márcio L V Araújo, Ivonete T L Queiroz, Romilson L Sampaio, Márcia S P L Souza, Ana Claudia F N da Silva, and Antonio C S Souza
- Subjects
Medicine ,Science - Abstract
In this paper, we provide a retrospective cohort study with patients that have been hospitalized for general or intensive care unit admission due to COVID-19, between March 3 and July 29, 2020, in the state of Bahia, Brazil. We aim to correlate those patients' demographics, symptoms and comorbidities, with the risk of mortality from COVID-19, length of hospital stay, and time from diagnosis to definitive outcome. On the basis of a dataset provided by the Health Secretary of the State of Bahia, we selected 3,896 hospitalized patients from a total of 154,868 COVID-19 patients that included non-hospitalized patients and patients with invalid registration in the dataset. Then, we statistically analyzed whether there was a significant correlation between the patient record data and the COVID-19 pandemic, and our main findings reinforced by the use of a multivariable logistic regression were that older age (Odds Ratio [OR] = 1.03, 95% Confidence Interval [CI] = 1.03-1.04, p-value (p)
- Published
- 2020
- Full Text
- View/download PDF
5. Citric-acid dialysate improves the calcification propensity of hemodialysis patients: A multicenter prospective randomized cross-over trial.
- Author
-
Karlien J Ter Meulen, Marijke J E Dekker, Andreas Pasch, Natascha J H Broers, Frank M van der Sande, Jeroen B van der Net, Constantijn J A M Konings, Isabelle M Gsponer, Matthias D N Bachtler, Adelheid Gauly, Bernard Canaud, and Jeroen P Kooman
- Subjects
Medicine ,Science - Abstract
IntroductionThe concentration of dialysate calcium (dCa) has been suggested to affect vascular calcification, but evidence is scarce. Calcification propensity reflects the intrinsic capacity of serum to prevent calcium and phosphate to precipitate. The use of citric-acid dialysate may have a beneficial effect on the calcification propensity due to the chelating effect on calcium and magnesium. The aim of this study was to compare the intradialytic and short-term effects of haemodialysis with either standard acetic-acid dialysate with dCa1.50 (A1.5) or dCa1.25 (A1.25), as well as citric-acid dialysate with dCa1.50 (C1.5) in bicarbonate dialysis on the calcification propensity of serum.MethodsChronic stable hemodialysis patients were included. This multicenter randomized cross-over study consisted out of a baseline week (A1.5), followed by the randomized sequence of A1.25 or C1.5 for one week after which the alternate treatment was provided after a washout week with A1.5. Calcification propensity of serum was assessed by time-resolved nephelometry where the T50 reflects the transition time between formation of primary and secondary calciprotein particles.ResultsEighteen patients (median age 70 years) completed the study. Intradialytic change in T50 was increased with C1.5 (121 [90-152]min) compared to A1.25 (83 [43-108]min, pConclusionCalcification propensity, as measured by the change in T50, improved significantly during treatment in C1.5 compared to A1.25 and A1.5. Long-term studies are needed to investigate the effects of different dialysate compositions concentrations on vascular calcification and bone mineral disorders.
- Published
- 2019
- Full Text
- View/download PDF
6. Incorporating public priorities in the Ocean Health Index: Canada as a case study.
- Author
-
Rémi M Daigle, Philippe Archambault, Benjamin S Halpern, Julia S Stewart Lowndes, and Isabelle M Côté
- Subjects
Medicine ,Science - Abstract
The Ocean Health Index (OHI) is a framework to assess ocean health by considering many benefits (called 'goals') provided by the ocean provides to humans, such as food provision, tourism opportunities, and coastal protection. The OHI framework can be used to assess marine areas at global or regional scales, but how various OHI goals should be weighted to reflect priorities at those scales remains unclear. In this study, we adapted the framework in two ways for application to Canada as a case study. First, we customized the OHI goals to create a national Canadian Ocean Health Index (COHI). In particular, we altered the list of iconic species assessed, added methane clathrates and subsea permafrost as carbon storage habitats, and developed a new goal, 'Aboriginal Needs', to measure access of Aboriginal people to traditional marine hunting and fishing grounds. Second, we evaluated various goal weighting schemes based on preferences elicited from the general public in online surveys. We quantified these public preferences in three ways: using Likert scores, simple ranks from a best-worst choice experiment, and model coefficients from the analysis of elicited choice experiment. The latter provided the clearest statistical discrimination among goals, and we recommend their use because they can more accurately reflect both public opinion and the trade-offs faced by policy-makers. This initial iteration of the COHI can be used as a baseline against which future COHI scores can be compared, and could potentially be used as a management tool to prioritise actions on a national scale and predict public support for these actions given that the goal weights are based on public priorities.
- Published
- 2017
- Full Text
- View/download PDF
7. Thrombocytopenia in neonatal sepsis: Incidence, severity and risk factors.
- Author
-
Isabelle M C Ree, Suzanne F Fustolo-Gunnink, Vincent Bekker, Karin J Fijnvandraat, Sylke J Steggerda, and Enrico Lopriore
- Subjects
Medicine ,Science - Abstract
Thrombocytopenia is a frequent problem in neonatal sepsis and is among the most predictive, independent risk factors for sepsis-associated mortality. This study aims to clarify the occurrence, severity and duration of thrombocytopenia in neonatal sepsis.A cohort study was carried out among all neonates with proven culture positive sepsis that were admitted to a tertiary NICU between 2006 and 2015 (n = 460). The occurrence, severity and duration of thrombocytopenia were recorded, as well as major bleedings and potential risk factors for mortality in neonatal sepsis.Sepsis was diagnosed in 460 of 6551 neonates (7%). Severe thrombocytopenia (platelets ≤50*109/L) occurred in 20% (92/460) of septic neonates. The median time for platelets to rise >100*109 was 6.0 days (interquartile range 4.0-7.0). On multivariate analysis, maternal hypertension, intravascular thrombosis and Gram negative (as opposed to Gram positive) sepsis were independently associated with thrombocytopenia in neonatal sepsis. In severe thrombocytopenia, 10% (9/92) suffered a severe IVH, compared to 5% (20/356) in neonates with platelets >50*109/L (p = 0.125). 10% (9/92) suffered a pulmonary hemorrhage, compared to 2% (9/368) in neonates with platelets >50*109/L (p = 0.001). On multivariate analysis, thrombocytopenia and Gram negative (as opposed to Gram positive) sepsis were independently associated with neonatal mortality.Thrombocytopenia is independently associated with maternal hypertension, intravascular thrombosis and Gram negative sepsis. Thrombocytopenia in neonatal sepsis increases the risk of mortality nearly four-fold, with another six-fold increase in mortality in case of Gram negative sepsis.
- Published
- 2017
- Full Text
- View/download PDF
8. Effects of Protection and Sediment Stress on Coral Reefs in Saint Lucia.
- Author
-
Chantale Bégin, Christiane K Schelten, Maggy M Nugues, Julie Hawkins, Callum Roberts, and Isabelle M Côté
- Subjects
Medicine ,Science - Abstract
The extent to which Marine Protected Areas (MPAs) benefit corals is contentious. On one hand, MPAs could enhance coral growth and survival through increases in herbivory within their borders; on the other, they are unlikely to prevent disturbances, such as terrestrial runoff, that originate outside their boundaries. We examined the effect of spatial protection and terrestrial sediment on the benthic composition of coral reefs in Saint Lucia. In 2011 (10 to 16 years after MPAs were created), we resurveyed 21 reefs that had been surveyed in 2001 and analyzed current benthic assemblages as well as changes in benthic cover over that decade in relation to protection status, terrestrial sediment influence (measured as the proportion of terrigenous material in reef-associated sediment) and depth. The cover of all benthic biotic components has changed significantly over the decade, including a decline in coral and increase in macroalgae. Protection status was not a significant predictor of either current benthic composition or changes in composition, but current cover and change in cover of several components were related to terrigenous content of sediment deposited recently. Sites with a higher proportion of terrigenous sediment had lower current coral cover, higher macroalgal cover and greater coral declines. Our results suggest that terrestrial sediment is an important factor in the recent degradation of coral reefs in Saint Lucia and that the current MPA network should be complemented by measures to reduce runoff from land.
- Published
- 2016
- Full Text
- View/download PDF
9. Dorsal Anterior Cingulate Thickness Is Related to Alexithymia in Childhood Trauma-Related PTSD.
- Author
-
Lauren A Demers, Elizabeth A Olson, David J Crowley, Scott L Rauch, and Isabelle M Rosso
- Subjects
Medicine ,Science - Abstract
Alexithymia, or "no words for feelings", is highly prevalent in samples with childhood maltreatment and posttraumatic stress disorder (PTSD). The dorsal anterior cingulate cortex (dACC) has been identified as a key region involved in alexithymia, early life trauma, and PTSD. Functional alterations in the dACC also have been associated with alexithymia in PTSD. This study examined whether dACC morphology is a neural correlate of alexithymia in child maltreatment-related PTSD. Sixteen adults with PTSD and a history of childhood sexual abuse, physical abuse, or exposure to domestic violence, and 24 healthy controls (HC) completed the Toronto Alexithymia Scale 20 (TAS-20) and underwent magnetic resonance imaging. Cortical thickness of the dACC was measured using FreeSurfer, and values were correlated with TAS-20 scores, controlling for sex and age, in both groups. Average TAS-20 score was significantly higher in the PTSD than the HC group. TAS-20 scores were significantly positively associated with dACC thickness only in the PTSD group. This association was strongest in the left hemisphere and for TAS-20 subscales that assess difficulty identifying and describing feelings. We found that increasing dACC gray matter thickness is a neural correlate of greater alexithymia in the context of PTSD with childhood maltreatment. While findings are correlational, they motivate further inquiry into the relationships between childhood adversity, emotional awareness and expression, and dACC morphologic development in trauma-related psychopathology.
- Published
- 2015
- Full Text
- View/download PDF
10. Simplification of Caribbean reef-fish assemblages over decades of coral reef degradation.
- Author
-
Lorenzo Alvarez-Filip, Michelle J Paddack, Ben Collen, D Ross Robertson, and Isabelle M Côté
- Subjects
Medicine ,Science - Abstract
Caribbean coral reefs are becoming structurally simpler, largely due to human impacts. The consequences of this trend for reef-associated communities are currently unclear, but expected to be profound. Here, we assess whether changes in fish assemblages have been non-random over several decades of declining reef structure. More specifically, we predicted that species that depend exclusively on coral reef habitat (i.e., habitat specialists) should be at a disadvantage compared to those that use a broader array of habitats (i.e., habitat generalists). Analysing 3727 abundance trends of 161 Caribbean reef-fishes, surveyed between 1980 and 2006, we found that the trends of habitat-generalists and habitat-specialists differed markedly. The abundance of specialists started to decline in the mid-1980s, reaching a low of ~60% of the 1980 baseline by the mid-1990s. Both the average and the variation in abundance of specialists have increased since the early 2000s, although the average is still well below the baseline level of 1980. This modest recovery occurred despite no clear evidence of a regional recovery in coral reef habitat quality in the Caribbean during the 2000s. In contrast, the abundance of generalist fishes remained relatively stable over the same three decades. Few specialist species are fished, thus their population declines are most likely linked to habitat degradation. These results mirror the observed trends of replacement of specialists by generalists, observed in terrestrial taxa across the globe. A significant challenge that arises from our findings is now to investigate if, and how, such community-level changes in fish populations affect ecosystem function.
- Published
- 2015
- Full Text
- View/download PDF
11. High-Throughput Analysis of T-DNA Location and Structure Using Sequence Capture.
- Author
-
Soichi Inagaki, Isabelle M Henry, Meric C Lieberman, and Luca Comai
- Subjects
Medicine ,Science - Abstract
Agrobacterium-mediated transformation of plants with T-DNA is used both to introduce transgenes and for mutagenesis. Conventional approaches used to identify the genomic location and the structure of the inserted T-DNA are laborious and high-throughput methods using next-generation sequencing are being developed to address these problems. Here, we present a cost-effective approach that uses sequence capture targeted to the T-DNA borders to select genomic DNA fragments containing T-DNA-genome junctions, followed by Illumina sequencing to determine the location and junction structure of T-DNA insertions. Multiple probes can be mixed so that transgenic lines transformed with different T-DNA types can be processed simultaneously, using a simple, index-based pooling approach. We also developed a simple bioinformatic tool to find sequence read pairs that span the junction between the genome and T-DNA or any foreign DNA. We analyzed 29 transgenic lines of Arabidopsis thaliana, each containing inserts from 4 different T-DNA vectors. We determined the location of T-DNA insertions in 22 lines, 4 of which carried multiple insertion sites. Additionally, our analysis uncovered a high frequency of unconventional and complex T-DNA insertions, highlighting the needs for high-throughput methods for T-DNA localization and structural characterization. Transgene insertion events have to be fully characterized prior to use as commercial products. Our method greatly facilitates the first step of this characterization of transgenic plants by providing an efficient screen for the selection of promising lines.
- Published
- 2015
- Full Text
- View/download PDF
12. What doesn't kill you makes you wary? Effect of repeated culling on the behaviour of an invasive predator.
- Author
-
Isabelle M Côté, Emily S Darling, Luis Malpica-Cruz, Nicola S Smith, Stephanie J Green, Jocelyn Curtis-Quick, and Craig Layman
- Subjects
Medicine ,Science - Abstract
As a result of being hunted, animals often alter their behaviour in ways that make future encounters with predators less likely. When hunting is carried out for conservation, for example to control invasive species, these behavioural changes can inadvertently impede the success of future efforts. We examined the effects of repeated culling by spearing on the behaviour of invasive predatory lionfish (Pterois volitans/miles) on Bahamian coral reef patches. We compared the extent of concealment and activity levels of lionfish at dawn and midday on 16 coral reef patches off Eleuthera, The Bahamas. Eight of the patches had been subjected to regular daytime removals of lionfish by spearing for two years. We also estimated the distance at which lionfish became alert to slowly approaching divers on culled and unculled reef patches. Lionfish on culled reefs were less active and hid deeper within the reef during the day than lionfish on patches where no culling had occurred. There were no differences at dawn when removals do not take place. Lionfish on culled reefs also adopted an alert posture at a greater distance from divers than lionfish on unculled reefs. More crepuscular activity likely leads to greater encounter rates by lionfish with more native fish species because the abundance of reef fish outside of shelters typically peaks at dawn and dusk. Hiding deeper within the reef could also make remaining lionfish less likely to be encountered and more difficult to catch by spearfishers during culling efforts. Shifts in the behaviour of hunted invasive animals might be common and they have implications both for the impact of invasive species and for the design and success of invasive control programs.
- Published
- 2014
- Full Text
- View/download PDF
13. The asymmetric binding of PGC-1α to the ERRα and ERRγ nuclear receptor homodimers involves a similar recognition mechanism.
- Author
-
Maria Takacs, Maxim V Petoukhov, R Andrew Atkinson, Pierre Roblin, François-Xavier Ogi, Borries Demeler, Noelle Potier, Yassmine Chebaro, Annick Dejaegere, Dmitri I Svergun, Dino Moras, and Isabelle M L Billas
- Subjects
Medicine ,Science - Abstract
BackgroundPGC-1α is a crucial regulator of cellular metabolism and energy homeostasis that functionally acts together with the estrogen-related receptors (ERRα and ERRγ) in the regulation of mitochondrial and metabolic gene networks. Dimerization of the ERRs is a pre-requisite for interactions with PGC-1α and other coactivators, eventually leading to transactivation. It was suggested recently (Devarakonda et al) that PGC-1α binds in a strikingly different manner to ERRγ ligand-binding domains (LBDs) compared to its mode of binding to ERRα and other nuclear receptors (NRs), where it interacts directly with the two ERRγ homodimer subunits.Methods/principal findingsHere, we show that PGC-1α receptor interacting domain (RID) binds in an almost identical manner to ERRα and ERRγ homodimers. Microscale thermophoresis demonstrated that the interactions between PGC-1α RID and ERR LBDs involve a single receptor subunit through high-affinity, ERR-specific L3 and low-affinity L2 interactions. NMR studies further defined the limits of PGC-1α RID that interacts with ERRs. Consistent with these findings, the solution structures of PGC-1α/ERRα LBDs and PGC-1α/ERRγ LBDs complexes share an identical architecture with an asymmetric binding of PGC-1α to homodimeric ERR.Conclusions/significanceThese studies provide the molecular determinants for the specificity of interactions between PGC-1α and the ERRs, whereby negative cooperativity prevails in the binding of the coactivators to these receptors. Our work indicates that allosteric regulation may be a general mechanism controlling the binding of the coactivators to homodimers.
- Published
- 2013
- Full Text
- View/download PDF
14. Native predators do not influence invasion success of pacific lionfish on Caribbean reefs.
- Author
-
Serena Hackerott, Abel Valdivia, Stephanie J Green, Isabelle M Côté, Courtney E Cox, Lad Akins, Craig A Layman, William F Precht, and John F Bruno
- Subjects
Medicine ,Science - Abstract
Biotic resistance, the process by which new colonists are excluded from a community by predation from and/or competition with resident species, can prevent or limit species invasions. We examined whether biotic resistance by native predators on Caribbean coral reefs has influenced the invasion success of red lionfishes (Pterois volitans and Pterois miles), piscivores from the Indo-Pacific. Specifically, we surveyed the abundance (density and biomass) of lionfish and native predatory fishes that could interact with lionfish (either through predation or competition) on 71 reefs in three biogeographic regions of the Caribbean. We recorded protection status of the reefs, and abiotic variables including depth, habitat type, and wind/wave exposure at each site. We found no relationship between the density or biomass of lionfish and that of native predators. However, lionfish densities were significantly lower on windward sites, potentially because of habitat preferences, and in marine protected areas, most likely because of ongoing removal efforts by reserve managers. Our results suggest that interactions with native predators do not influence the colonization or post-establishment population density of invasive lionfish on Caribbean reefs.
- Published
- 2013
- Full Text
- View/download PDF
15. Face your fears: cleaning gobies inspect predators despite being stressed by them.
- Author
-
Marta C Soares, Redouan Bshary, Sónia C Cardoso, Isabelle M Côté, and Rui F Oliveira
- Subjects
Medicine ,Science - Abstract
social stressors typically elicit two distinct behavioural responses in vertebrates: an active response (i.e., "fight or flight") or behavioural inhibition (i.e., freezing). Here, we report an interesting exception to this dichotomy in a Caribbean cleaner fish, which interacts with a wide variety of reef fish clients, including predatory species. Cleaning gobies appraise predatory clients as potential threat and become stressed in their presence, as evidenced by their higher cortisol levels when exposed to predatory rather than to non-predatory clients. Nevertheless, cleaning gobies neither flee nor freeze in response to dangerous clients but instead approach predators faster (both in captivity and in the wild), and interact longer with these clients than with non-predatory clients (in the wild). We hypothesise that cleaners interrupt the potentially harmful physiological consequences elicited by predatory clients by becoming increasingly proactive and by reducing the time elapsed between client approach and the start of the interaction process. The activation of a stress response may therefore also be responsible for the longer cleaning service provided by these cleaners to predatory clients in the wild. Future experimental studies may reveal similar patterns in other social vertebrate species when, for instance, individuals approach an opponent for reconciliation after a conflict.
- Published
- 2012
- Full Text
- View/download PDF
16. Identification of combinatorial patterns of post-translational modifications on individual histones in the mouse brain.
- Author
-
Ry Y Tweedie-Cullen, Andrea M Brunner, Jonas Grossmann, Safa Mohanna, David Sichau, Paolo Nanni, Christian Panse, and Isabelle M Mansuy
- Subjects
Medicine ,Science - Abstract
Post-translational modifications (PTMs) of proteins are biochemical processes required for cellular functions and signalling that occur in every sub-cellular compartment. Multiple protein PTMs exist, and are established by specific enzymes that can act in basal conditions and upon cellular activity. In the nucleus, histone proteins are subjected to numerous PTMs that together form a histone code that contributes to regulate transcriptional activity and gene expression. Despite their importance however, histone PTMs have remained poorly characterised in most tissues, in particular the brain where they are thought to be required for complex functions such as learning and memory formation. Here, we report the comprehensive identification of histone PTMs, of their combinatorial patterns, and of the rules that govern these patterns in the adult mouse brain. Based on liquid chromatography, electron transfer, and collision-induced dissociation mass spectrometry, we generated a dataset containing a total of 10,646 peptides from H1, H2A, H2B, H3, H4, and variants in the adult brain. 1475 of these peptides carried one or more PTMs, including 141 unique sites and a total of 58 novel sites not described before. We observed that these PTMs are not only classical modifications such as serine/threonine (Ser/Thr) phosphorylation, lysine (Lys) acetylation, and Lys/arginine (Arg) methylation, but also include several atypical modifications such as Ser/Thr acetylation, and Lys butyrylation, crotonylation, and propionylation. Using synthetic peptides, we validated the presence of these atypical novel PTMs in the mouse brain. The application of data-mining algorithms further revealed that histone PTMs occur in specific combinations with different ratios. Overall, the present data newly identify a specific histone code in the mouse brain and reveal its level of complexity, suggesting its potential relevance for higher-order brain functions.
- Published
- 2012
- Full Text
- View/download PDF
17. Selective regulation of NR2B by protein phosphatase-1 for the control of the NMDA receptor in neuroprotection.
- Author
-
Mélissa Farinelli, Fabrice D Heitz, Benjamin F Grewe, Shiva K Tyagarajan, Fritjof Helmchen, and Isabelle M Mansuy
- Subjects
Medicine ,Science - Abstract
An imbalance between pro-survival and pro-death pathways in brain cells can lead to neuronal cell death and neurodegeneration. While such imbalance is known to be associated with alterations in glutamatergic and Ca(2+) signaling, the underlying mechanisms remain undefined. We identified the protein Ser/Thr phosphatase protein phosphatase-1 (PP1), an enzyme associated with glutamate receptors, as a key trigger of survival pathways that can prevent neuronal death and neurodegeneration in the adult hippocampus. We show that PP1α overexpression in hippocampal neurons limits NMDA receptor overactivation and Ca(2+) overload during an excitotoxic event, while PP1 inhibition favors Ca(2+) overload and cell death. The protective effect of PP1 is associated with a selective dephosphorylation on a residue phosphorylated by CaMKIIα on the NMDA receptor subunit NR2B, which promotes pro-survival pathways and associated transcriptional programs. These results reveal a novel contributor to the mechanisms of neuroprotection and underscore the importance of PP1-dependent dephosphorylation in these mechanisms. They provide a new target for the development of potential therapeutic treatment of neurodegeneration.
- Published
- 2012
- Full Text
- View/download PDF
18. Invasive lionfish drive Atlantic coral reef fish declines.
- Author
-
Stephanie J Green, John L Akins, Aleksandra Maljković, and Isabelle M Côté
- Subjects
Medicine ,Science - Abstract
Indo-Pacific lionfish (Pterois volitans and P. miles) have spread swiftly across the Western Atlantic, producing a marine predator invasion of unparalleled speed and magnitude. There is growing concern that lionfish will affect the structure and function of invaded marine ecosystems, however detrimental impacts on natural communities have yet to be measured. Here we document the response of native fish communities to predation by lionfish populations on nine coral reefs off New Providence Island, Bahamas. We assessed lionfish diet through stomach contents analysis, and quantified changes in fish biomass through visual surveys of lionfish and native fishes at the sites over time. Lionfish abundance increased rapidly between 2004 and 2010, by which time lionfish comprised nearly 40% of the total predator biomass in the system. The increase in lionfish abundance coincided with a 65% decline in the biomass of the lionfish's 42 Atlantic prey fishes in just two years. Without prompt action to control increasing lionfish populations, similar effects across the region may have long-term negative implications for the structure of Atlantic marine communities, as well as the societies and economies that depend on them.
- Published
- 2012
- Full Text
- View/download PDF
19. Influence of early stress on social abilities and serotonergic functions across generations in mice.
- Author
-
Tamara B Franklin, Natacha Linder, Holger Russig, Beat Thöny, and Isabelle M Mansuy
- Subjects
Medicine ,Science - Abstract
Exposure to adverse environments during early development is a known risk factor for several psychiatric conditions including antisocial behavior and personality disorders. Here, we induced social anxiety and altered social recognition memory in adult mice using unpredictable maternal separation and maternal stress during early postnatal life. We show that these social defects are not only pronounced in the animals directly subjected to stress, but are also transmitted to their offspring across two generations. The defects are associated with impaired serotonergic signaling, in particular, reduced 5HT1A receptor expression in the dorsal raphe nucleus, and increased serotonin level in a dorsal raphe projection area. These findings underscore the susceptibility of social behaviors and serotonergic pathways to early stress, and the persistence of their perturbation across generations.
- Published
- 2011
- Full Text
- View/download PDF
20. Genome characterization of the oleaginous fungus Mortierella alpina.
- Author
-
Lei Wang, Wei Chen, Yun Feng, Yan Ren, Zhennan Gu, Haiqin Chen, Hongchao Wang, Michael J Thomas, Baixi Zhang, Isabelle M Berquin, Yang Li, Jiansheng Wu, Huanxin Zhang, Yuanda Song, Xiang Liu, James S Norris, Suriguga Wang, Peng Du, Junguo Shen, Na Wang, Yanlin Yang, Wei Wang, Lu Feng, Colin Ratledge, Hao Zhang, and Yong Q Chen
- Subjects
Medicine ,Science - Abstract
Mortierella alpina is an oleaginous fungus which can produce lipids accounting for up to 50% of its dry weight in the form of triacylglycerols. It is used commercially for the production of arachidonic acid. Using a combination of high throughput sequencing and lipid profiling, we have assembled the M. alpina genome, mapped its lipogenesis pathway and determined its major lipid species. The 38.38 Mb M. alpina genome shows a high degree of gene duplications. Approximately 50% of its 12,796 gene models, and 60% of genes in the predicted lipogenesis pathway, belong to multigene families. Notably, M. alpina has 18 lipase genes, of which 11 contain the class 2 lipase domain and may share a similar function. M. alpina's fatty acid synthase is a single polypeptide containing all of the catalytic domains required for fatty acid synthesis from acetyl-CoA and malonyl-CoA, whereas in many fungi this enzyme is comprised of two polypeptides. Major lipids were profiled to confirm the products predicted in the lipogenesis pathway. M. alpina produces a complex mixture of glycerolipids, glycerophospholipids and sphingolipids. In contrast, only two major sterol lipids, desmosterol and 24(28)-methylene-cholesterol, were detected. Phylogenetic analysis based on genes involved in lipid metabolism suggests that oleaginous fungi may have acquired their lipogenic capacity during evolution after the divergence of Ascomycota, Basidiomycota, Chytridiomycota and Mucoromycota. Our study provides the first draft genome and comprehensive lipid profile for M. alpina, and lays the foundation for possible genetic engineering of M. alpina to produce higher levels and diverse contents of dietary lipids.
- Published
- 2011
- Full Text
- View/download PDF
21. Protein phosphatase 1 (PP1) is a post-translational regulator of the mammalian circadian clock.
- Author
-
Isabelle Schmutz, Sabrina Wendt, Anna Schnell, Achim Kramer, Isabelle M Mansuy, and Urs Albrecht
- Subjects
Medicine ,Science - Abstract
Circadian clocks coordinate the timing of important biological processes. Interconnected transcriptional and post-translational feedback loops based on a set of clock genes generate and maintain these rhythms with a period of about 24 hours. Many clock proteins undergo circadian cycles of post-translational modifications. Among these modifications, protein phosphorylation plays an important role in regulating activity, stability and intracellular localization of clock components. Several protein kinases were characterized as regulators of the circadian clock. However, the function of protein phosphatases, which balance phosphorylation events, in the mammalian clock mechanism is less well understood. Here, we identify protein phosphatase 1 (PP1) as regulator of period and light-induced resetting of the mammalian circadian clock. Down-regulation of PP1 activity in cells by RNA interference and in vivo by expression of a specific inhibitor in the brain of mice tended to lengthen circadian period. Moreover, reduction of PP1 activity in the brain altered light-mediated clock resetting behavior in mice, enhancing the phase shifts in either direction. At the molecular level, diminished PP1 activity increased nuclear accumulation of the clock component PER2 in neurons. Hence, PP1, may reduce PER2 phosphorylation thereby influencing nuclear localization of this protein. This may at least partially influence period and phase shifting properties of the mammalian circadian clock.
- Published
- 2011
- Full Text
- View/download PDF
22. Searching for speciation genes: molecular evidence for selection associated with colour morphotypes in the Caribbean reef fish genus Hypoplectrus.
- Author
-
Ben G Holt, Isabelle M Côté, and Brent C Emerson
- Subjects
Medicine ,Science - Abstract
Closely related species that show clear phenotypic divergence, but without obvious geographic barriers, can provide opportunities to study how diversification can occur when opportunities for allopatric speciation are limited. We examined genetic divergence in the coral reef fish genus Hypoplectrus (family: Serranidae), which comprises of 10-14 morphotypes that are distinguished solely by their distinct colour patterns, but which show little genetic differentiation. Our goal was to detect loci that show clear disequilibrium between morphotypes and across geographical locations. We conducted Amplified Fragment Length Polymorphism molecular analysis to quantify genetic differentiation among, and selection between, morphotypes. Three loci were consistently divergent beyond neutral expectations in repeated pair-wise morphotype comparisons using two different methods. These loci provide the first evidence for genes that may be associated with colour morphotype in the genus Hypoplectrus.
- Published
- 2011
- Full Text
- View/download PDF
23. The comparison between circadian oscillators in mouse liver and pituitary gland reveals different integration of feeding and light schedules.
- Author
-
Isabelle M Bur, Sonia Zouaoui, Pierre Fontanaud, Nathalie Coutry, François Molino, Agnès O Martin, Patrice Mollard, and Xavier Bonnefont
- Subjects
Medicine ,Science - Abstract
The mammalian circadian system is composed of multiple peripheral clocks that are synchronized by a central pacemaker in the suprachiasmatic nuclei of the hypothalamus. This system keeps track of the external world rhythms through entrainment by various time cues, such as the light-dark cycle and the feeding schedule. Alterations of photoperiod and meal time modulate the phase coupling between central and peripheral oscillators. In this study, we used real-time quantitative PCR to assess circadian clock gene expression in the liver and pituitary gland from mice raised under various photoperiods, or under a temporal restricted feeding protocol. Our results revealed unexpected differences between both organs. Whereas the liver oscillator always tracked meal time, the pituitary circadian clockwork showed an intermediate response, in between entrainment by the light regimen and the feeding-fasting rhythm. The same composite response was also observed in the pituitary gland from adrenalectomized mice under daytime restricted feeding, suggesting that circulating glucocorticoids do not inhibit full entrainment of the pituitary clockwork by meal time. Altogether our results reveal further aspects in the complexity of phase entrainment in the circadian system, and suggest that the pituitary may host oscillators able to integrate multiple time cues.
- Published
- 2010
- Full Text
- View/download PDF
24. Rescue of salivary gland function after stem cell transplantation in irradiated glands.
- Author
-
Isabelle M A Lombaert, Jeanette F Brunsting, Pieter K Wierenga, Hette Faber, Monique A Stokman, Tineke Kok, Willy H Visser, Harm H Kampinga, Gerald de Haan, and Robert P Coppes
- Subjects
Medicine ,Science - Abstract
Head and neck cancer is the fifth most common malignancy and accounts for 3% of all new cancer cases each year. Despite relatively high survival rates, the quality of life of these patients is severely compromised because of radiation-induced impairment of salivary gland function and consequential xerostomia (dry mouth syndrome). In this study, a clinically applicable method for the restoration of radiation-impaired salivary gland function using salivary gland stem cell transplantation was developed. Salivary gland cells were isolated from murine submandibular glands and cultured in vitro as salispheres, which contained cells expressing the stem cell markers Sca-1, c-Kit and Musashi-1. In vitro, the cells differentiated into salivary gland duct cells and mucin and amylase producing acinar cells. Stem cell enrichment was performed by flow cytrometric selection using c-Kit as a marker. In vitro, the cells differentiated into amylase producing acinar cells. In vivo, intra-glandular transplantation of a small number of c-Kit(+) cells resulted in long-term restoration of salivary gland morphology and function. Moreover, donor-derived stem cells could be isolated from primary recipients, cultured as secondary spheres and after re-transplantation ameliorate radiation damage. Our approach is the first proof for the potential use of stem cell transplantation to functionally rescue salivary gland deficiency.
- Published
- 2008
- Full Text
- View/download PDF
25. Controlled experiment finds no detectable citation bump from Twitter promotion
- Author
-
Branch, Trevor A., primary, Cȏté, Isabelle M., additional, David, Solomon R., additional, Drew, Joshua A., additional, LaRue, Michelle, additional, Márquez, Melissa C., additional, Parsons, E. C. M., additional, Rabaiotti, D., additional, Shiffman, David, additional, Steen, David A., additional, and Wild, Alexander L., additional
- Published
- 2024
- Full Text
- View/download PDF
26. High-Throughput Analysis of T-DNA Location and Structure Using Sequence Capture.
- Author
-
Inagaki, Soichi, Henry, Isabelle M, Lieberman, Meric C, and Comai, Luca
- Subjects
Plants ,Genetically Modified ,Arabidopsis ,DNA ,Bacterial ,DNA ,Plant ,Mutagenesis ,Insertional ,Sequence Analysis ,DNA ,Transformation ,Genetic ,Genome ,Plant ,High-Throughput Nucleotide Sequencing ,Agrobacterium tumefaciens ,DNA ,Bacterial ,Plant ,Genome ,Mutagenesis ,Insertional ,Plants ,Genetically Modified ,Sequence Analysis ,Transformation ,Genetic ,General Science & Technology - Abstract
Agrobacterium-mediated transformation of plants with T-DNA is used both to introduce transgenes and for mutagenesis. Conventional approaches used to identify the genomic location and the structure of the inserted T-DNA are laborious and high-throughput methods using next-generation sequencing are being developed to address these problems. Here, we present a cost-effective approach that uses sequence capture targeted to the T-DNA borders to select genomic DNA fragments containing T-DNA-genome junctions, followed by Illumina sequencing to determine the location and junction structure of T-DNA insertions. Multiple probes can be mixed so that transgenic lines transformed with different T-DNA types can be processed simultaneously, using a simple, index-based pooling approach. We also developed a simple bioinformatic tool to find sequence read pairs that span the junction between the genome and T-DNA or any foreign DNA. We analyzed 29 transgenic lines of Arabidopsis thaliana, each containing inserts from 4 different T-DNA vectors. We determined the location of T-DNA insertions in 22 lines, 4 of which carried multiple insertion sites. Additionally, our analysis uncovered a high frequency of unconventional and complex T-DNA insertions, highlighting the needs for high-throughput methods for T-DNA localization and structural characterization. Transgene insertion events have to be fully characterized prior to use as commercial products. Our method greatly facilitates the first step of this characterization of transgenic plants by providing an efficient screen for the selection of promising lines.
- Published
- 2015
27. Correction: Correlation between hospitalized patients’ demographics, symptoms, comorbidities, and COVID-19 pandemic in Bahia, Brazil
- Author
-
Macedo, Márcio C. F., primary, Pinheiro, Isabelle M., additional, Carvalho, Caio J. L., additional, Fraga, Hilda C. J. R., additional, Araujo, Isaac P. C., additional, Montes, Simone S., additional, Araujo, Otávio A. C., additional, Alves, Lucas A., additional, Saba, Hugo, additional, Araújo, Márcio L. V., additional, Queiroz, Ivonete T. L., additional, Sampaio, Romilson L., additional, Souza, Márcia S. P. L., additional, da Silva, Ana Claudia F. N., additional, and Souza, Antonio C. S., additional
- Published
- 2021
- Full Text
- View/download PDF
28. Sex-specific expression profiles of ecdysteroid biosynthesis and ecdysone response genes in extreme sexual dimorphism of the mealybug Planococcus kraunhiae (Kuwana)
- Author
-
Miyuki Muramatsu, Tomohiro Tsuji, Isabelle M. Vea, Chieka Minakuchi, Ken Miura, Akiya Jouraku, Takahiro Shiotsuki, and Sayumi Tanaka
- Subjects
Male ,0106 biological sciences ,0301 basic medicine ,Life Cycles ,Insecta ,Physiology ,Gene Expression ,Molting ,01 natural sciences ,Database and Informatics Methods ,chemistry.chemical_compound ,Cytochrome P-450 Enzyme System ,Morphogenesis ,Medicine and Health Sciences ,Wings, Animal ,media_common ,Sex Characteristics ,Sexual Differentiation ,Insect Metamorphosis ,Multidisciplinary ,integumentary system ,Metamorphosis, Biological ,Pupa ,Gene Expression Regulation, Developmental ,Eukaryota ,Prothoracic gland ,Juvenile Hormones ,Insects ,Ecdysterone ,Larva ,Insect Proteins ,Medicine ,Female ,Sequence Analysis ,Ecdysone ,Research Article ,medicine.medical_specialty ,animal structures ,Arthropoda ,Bioinformatics ,media_common.quotation_subject ,Science ,Sequence Databases ,Biology ,Research and Analysis Methods ,010603 evolutionary biology ,Halloween genes ,03 medical and health sciences ,Internal medicine ,Genetics ,medicine ,Animals ,Juvenile ,Metamorphosis ,Ecdysteroid ,Sexual Dimorphism ,fungi ,Organisms ,Ecdysteroids ,Biology and Life Sciences ,Invertebrates ,Nymphs ,Biological Databases ,030104 developmental biology ,Endocrinology ,chemistry ,Juvenile hormone ,Physiological Processes ,Ecdysone receptor ,Sequence Alignment ,Zoology ,Entomology ,Developmental Biology - Abstract
Insect molting hormone (ecdysteroids) and juvenile hormone regulate molting and metamorphic events in a variety of insect species. Mealybugs undergo sexually dimorphic metamorphosis: males develop into winged adults through non-feeding, pupa-like stages called prepupa and pupa, while females emerge as neotenic wingless adults. We previously demonstrated, in the Japanese mealybug Planococcus kraunhiae (Kuwana), that the juvenile hormone titer is higher in males than in females at the end of the juvenile stage, which suggests that juvenile hormone may regulate male-specific adult morphogenesis. Here, we examined the involvement of ecdysteroids in sexually dimorphic metamorphosis. To estimate ecdysteroid titers, quantitative RT-PCR analyses of four Halloween genes encoding for cytochrome P450 monooxygenases in ecdysteroid biosynthesis, i.e., spook, disembodied, shadow and shade, were performed. Overall, their expression levels peaked before each nymphal molt. Transcript levels of spook, disembodied and shadow, genes that catalyze the steps in ecdysteroid biosynthesis in the prothoracic gland, were higher in males from the middle of the second nymphal instar to adult emergence. In contrast, the expression of shade, which was reported to be involved in the conversion of ecdysone into 20-hydroxyecdysone in peripheral tissues, was similar between males and females. These results suggest that ecdysteroid biosynthesis in the prothoracic gland is more active in males than in females, although the final conversion into 20-hydroxyecdysone occurs at similar levels in both sexes. Moreover, expression profiles of ecdysone response genes, ecdysone receptor and ecdysone-induced protein 75B, were also analyzed. Based on these expression profiles, we propose that the changes in ecdysteroid titer differ between males and females, and that high ecdysteroid titer is essential for directing male adult development.
- Published
- 2020
29. Identifying causative mechanisms linking early-life stress to psycho-cardio-metabolic multi-morbidity: The EarlyCause project
- Author
-
Mariani, Nicole, primary, Borsini, Alessandra, additional, Cecil, Charlotte A. M., additional, Felix, Janine F., additional, Sebert, Sylvain, additional, Cattaneo, Annamaria, additional, Walton, Esther, additional, Milaneschi, Yuri, additional, Cochrane, Guy, additional, Amid, Clara, additional, Rajan, Jeena, additional, Giacobbe, Juliette, additional, Sanz, Yolanda, additional, Agustí, Ana, additional, Sorg, Tania, additional, Herault, Yann, additional, Miettunen, Jouko, additional, Parmar, Priyanka, additional, Cattane, Nadia, additional, Jaddoe, Vincent, additional, Lötjönen, Jyrki, additional, Buisan, Carme, additional, González Ballester, Miguel A., additional, Piella, Gemma, additional, Gelpi, Josep L., additional, Lamers, Femke, additional, Penninx, Brenda W. J. H., additional, Tiemeier, Henning, additional, von Tottleben, Malte, additional, Thiel, Rainer, additional, Heil, Katharina F., additional, Järvelin, Marjo-Riitta, additional, Pariante, Carmine, additional, Mansuy, Isabelle M., additional, and Lekadir, Karim, additional
- Published
- 2021
- Full Text
- View/download PDF
30. Correlation between hospitalized patients’ demographics, symptoms, comorbidities, and COVID-19 pandemic in Bahia, Brazil
- Author
-
Macedo, Márcio C. F., primary, Pinheiro, Isabelle M., additional, Carvalho, Caio J. L., additional, Fraga, Hilda C. J. R., additional, Araujo, Isaac P. C., additional, Montes, Simone S., additional, Araujo, Otávio A. C., additional, Alves, Lucas A., additional, Saba, Hugo, additional, Araújo, Márcio L. V., additional, Queiroz, Ivonete T. L., additional, Sampaio, Romilson L., additional, Souza, Márcia S. P. L., additional, da Silva, Ana Claudia F. N., additional, and Souza, Antonio C. S., additional
- Published
- 2020
- Full Text
- View/download PDF
31. Citric-acid dialysate improves the calcification propensity of hemodialysis patients: A multicenter prospective randomized cross-over trial
- Author
-
ter Meulen, Karlien J., primary, Dekker, Marijke J. E., additional, Pasch, Andreas, additional, Broers, Natascha J. H., additional, van der Sande, Frank M., additional, van der Net, Jeroen B., additional, Konings, Constantijn J. A. M., additional, Gsponer, Isabelle M., additional, Bachtler, Matthias D. N., additional, Gauly, Adelheid, additional, Canaud, Bernard, additional, and Kooman, Jeroen P., additional
- Published
- 2019
- Full Text
- View/download PDF
32. Citric-acid dialysate improves the calcification propensity of hemodialysis patients: A multicenter prospective randomized cross-over trial
- Author
-
Bernard Canaud, Andreas Pasch, Adelheid Gauly, Jeroen P. Kooman, Isabelle M Gsponer, Jeroen B van der Net, Karlien J Ter Meulen, Frank M. van der Sande, Natascha J. H. Broers, Constantijn J.A.M. Konings, Matthias Bachtler, Marijke J E Dekker, MUMC+: MA Nefrologie (9), MUMC+: MA Med Staf Artsass Interne Geneeskunde (9), Interne Geneeskunde, RS: NUTRIM - R3 - Respiratory & Age-related Health, RS: Carim - V02 Hypertension and target organ damage, and RS: CARIM other
- Subjects
Male ,Time Factors ,Physiology ,medicine.medical_treatment ,030232 urology & nephrology ,Hemodynamics ,030204 cardiovascular system & hematology ,Cardiovascular Medicine ,law.invention ,0302 clinical medicine ,Randomized controlled trial ,MAINTENANCE HEMODIALYSIS ,law ,Medicine and Health Sciences ,Renal Failure ,Prospective Studies ,Prospective cohort study ,ALL-CAUSE MORTALITY ,Statistical Data ,Bone mineral ,OUTCOMES ,Multidisciplinary ,CALCIUM CONCENTRATIONS ,Statistics ,STAGE RENAL-DISEASE ,STIFFNESS ,Hematology ,Chemistry ,Nephrology ,Cardiovascular Diseases ,BALANCE ,Physical Sciences ,Medicine ,Female ,Hemodialysis ,Research Article ,medicine.medical_specialty ,Science ,PATIENTS RECEIVING HEMODIALYSIS ,Urology ,chemistry.chemical_element ,Calcium ,Citric Acid ,Calcification ,Phosphates ,CITRATE DIALYSATE ,03 medical and health sciences ,Renal Dialysis ,Medical Dialysis ,medicine ,Humans ,Vascular Calcification ,Aged ,business.industry ,Chemical Compounds ,Biology and Life Sciences ,medicine.disease ,Crossover study ,Bicarbonates ,chemistry ,business ,Physiological Processes ,Mathematics - Abstract
INTRODUCTION: The concentration of dialysate calcium (dCa) has been suggested to affect vascular calcification, but evidence is scarce. Calcification propensity reflects the intrinsic capacity of serum to prevent calcium and phosphate to precipitate. The use of citric-acid dialysate may have a beneficial effect on the calcification propensity due to the chelating effect on calcium and magnesium. The aim of this study was to compare the intradialytic and short-term effects of haemodialysis with either standard acetic-acid dialysate with dCa1.50 (A1.5) or dCa1.25 (A1.25), as well as citric-acid dialysate with dCa1.50 (C1.5) in bicarbonate dialysis on the calcification propensity of serum.METHODS: Chronic stable hemodialysis patients were included. This multicenter randomized cross-over study consisted out of a baseline week (A1.5), followed by the randomized sequence of A1.25 or C1.5 for one week after which the alternate treatment was provided after a washout week with A1.5. Calcification propensity of serum was assessed by time-resolved nephelometry where the T50 reflects the transition time between formation of primary and secondary calciprotein particles.RESULTS: Eighteen patients (median age 70 years) completed the study. Intradialytic change in T50 was increased with C1.5 (121 [90-152]min) compared to A1.25 (83 [43-108]min, pCONCLUSION: Calcification propensity, as measured by the change in T50, improved significantly during treatment in C1.5 compared to A1.25 and A1.5. Long-term studies are needed to investigate the effects of different dialysate compositions concentrations on vascular calcification and bone mineral disorders.
- Published
- 2019
33. Correction: Medico-economic comparison of two anticoagulant treatment strategies: Vitamin K antagonists vs. direct oral anticoagulants in older adults in nursing homes in France. The 'MIKADO' study.
- Author
-
George Pisica-Donose, Matthieu Piccoli, Bastien Genet, Stéphane Bouee, Stefan Berechet, Ion Berechet, Antonin Dacasa Cortes, Sabri Atsamena, Catherine Bayle, Mihai Badescu, François Catelain, Lynda Kermeche, Isabelle Merlier, Sahondranirina Rakotoniary, Valérie Savin, Ariane Vidal, Jean-Sébastien Vidal, and Olivier Hanon
- Subjects
Medicine ,Science - Abstract
[This corrects the article DOI: 10.1371/journal.pone.0283604.].
- Published
- 2024
- Full Text
- View/download PDF
34. The relationship between participation in leisure activities and incidence of falls in residential aged care.
- Author
-
Guogui Huang, Nasir Wabe, Magdalena Z Raban, S Sandun Malpriya Silva, Karla Seaman, Amy D Nguyen, Isabelle Meulenbroeks, and Johanna I Westbrook
- Subjects
Medicine ,Science - Abstract
BackgroundActive engagement in leisure activities has positive effects on individuals' health outcomes and social functioning; however, there is limited understanding of the link between participation in leisure activities, particularly non-exercise activities, and falls in older adults. This study aimed to determine the relationship between participation in leisure activities and the incidence of falls, and the variation of this relationship by dementia status in residential aged care facilities (RACFs).MethodsA retrospective longitudinal cohort study utilising routinely collected data (January 2021-August 2022) from 25 RACFs in Sydney, Australia, was conducted. The cohort included 3,024 older permanent residents (1,493 with dementia and 1,531 without) aged ≥65 and with a stay of ≥1 week. The level of participation in leisure activities was measured using the number of leisure activities per 1,000 resident days and divided into quartiles. Outcome measures were the incidence rate of all falls and injurious falls (i.e., number of falls per 1,000 resident days). We used multilevel negative binary regression to examine the relationship between leisure participation and fall incidence.ResultsFor the whole sample, leisure participation was significantly inversely associated with the incidence rate of all falls and injurious falls. For example, residents in the high leisure participation group were 26% less likely to experience a fall compared to those in the low leisure participation group after controlling for confounders (incidence rate ratio = 0.74, 95% confidence interval = 0.60, 0.91). Such inverse relationship was observed in both exercise and non-exercise activities and was stronger among residents without dementia.ConclusionsLeisure participation is associated with a lower rate of falls, a key quality indicator by which RACFs are benchmarked and funded in Australia and many other countries. More recognition and attention are needed for the currently underfunded leisure activities in RACFs in future funding arrangement.
- Published
- 2024
- Full Text
- View/download PDF
35. Evaluation of radiological capacity and usage in paediatric TB diagnosis: A mixed-method protocol of a comparative study in Mozambique, South Africa and Spain.
- Author
-
Isabelle Munyangaju, Benedita José, Ridwaan Esmail, Megan Palmer, Begoña Santiago, Alicia Hernanz-Lobo, Crimenia Mutemba, Patricia Perez, Liebe Hendrietta Tlhapi, Vanessa Mudaly, Richard D Pitcher, Andreas Jahnen, Eliseo Vañó Carruana, Elisa López-Varela, and Isabelle Thierry-Chef
- Subjects
Medicine ,Science - Abstract
IntroductionTuberculosis remains one of the top ten causes of mortality globally. Children accounted for 12% of all TB cases and 18% of all TB deaths in 2022. Paediatric TB is difficult to diagnose with conventional laboratory tests, and chest radiographs remain crucial. However, in low-and middle-income countries with high TB burden, the capacity for radiological diagnosis of paediatric TB is rarely documented and data on the associated radiation exposure limited.MethodsA multicentre, mixed-methods study is proposed in three countries, Mozambique, South Africa and Spain. At the national level, official registry databases will be utilised to retrospectively compile an inventory of licensed imaging resources (mainly X-ray and Computed Tomography (CT) scan equipment) for the year 2021. At the selected health facility level, three descriptive cross-sectional standardised surveys will be conducted to assess radiology capacity, radiological imaging diagnostic use for paediatric TB diagnosis, and radiation protection optimization: a site survey, a clinician-targeted survey, and a radiology staff-targeted survey, respectively. At the patient level, potential dose optimisation will be assessed for children under 16 years of age who were diagnosed and treated for TB in selected sites in each country. For this component, a retrospective analysis of dosimetry will be performed on TB and radiology data routinely collected at the respective sites. National inventory data will be presented as the number of units per million people by modality, region and country. Descriptive analyses will be conducted on survey data, including the demographic, clinical and programmatic characteristics of children treated for TB who had imaging examinations (chest X-ray (CXR) and/or CT scan). Dose exposure analysis will be performed by children's age, gender and disease spectrum.DiscussionAs far as we know, this is the first multicentre and multi-national study to compare radiological capacity, radiation protection optimization and practices between high and low TB burden settings in the context of childhood TB management. The planned comparative analyses will inform policy-makers of existing radiological capacity and deficiencies, allowing better resource prioritisation. It will inform clinicians and radiologists on best practices and means to optimise the use of radiological technology in paediatric TB management.
- Published
- 2024
- Full Text
- View/download PDF
36. Incorporating public priorities in the Ocean Health Index: Canada as a case study
- Author
-
Isabelle M. Côté, Julia S. Stewart Lowndes, Philippe Archambault, Benjamin S. Halpern, and Rémi M. Daigle
- Subjects
0106 biological sciences ,010504 meteorology & atmospheric sciences ,Glaciology ,Marine and Aquatic Sciences ,lcsh:Medicine ,Public opinion ,01 natural sciences ,Geographical locations ,Surveys and Questionnaires ,Oceans ,Medicine and Health Sciences ,lcsh:Science ,Multidisciplinary ,Ecology ,Sea Ice ,Environmental resource management ,Marine Ecology ,Agriculture ,Biodiversity ,Chemistry ,Geography ,Scale (social sciences) ,Physical Sciences ,Environmental Health ,Methane ,Statistical discrimination ,Research Article ,Canada ,Conservation of Natural Resources ,Oceans and Seas ,Fishing ,Fisheries ,Marine Biology ,010603 evolutionary biology ,Likert scale ,Humans ,14. Life underwater ,Baseline (configuration management) ,American Indian or Alaska Native ,Ecosystem ,Nutrition ,0105 earth and related environmental sciences ,Health Priorities ,business.industry ,Ecology and Environmental Sciences ,lcsh:R ,Chemical Compounds ,Biology and Life Sciences ,Bodies of Water ,15. Life on land ,Diet ,Weighting ,Food ,13. Climate action ,Public Opinion ,North America ,Earth Sciences ,lcsh:Q ,People and places ,business ,Tourism - Abstract
The Ocean Health Index (OHI) is a framework to assess ocean health by considering many benefits (called ‘goals’) provided by the ocean provides to humans, such as food provision, tourism opportunities, and coastal protection. The OHI framework can be used to assess marine areas at global or regional scales, but how various OHI goals should be weighted to reflect priorities at those scales remains unclear. In this study, we adapted the framework in two ways for application to Canada as a case study. First, we customized the OHI goals to create a national Canadian Ocean Health Index (COHI). In particular, we altered the list of iconic species assessed, added methane clathrates and subsea permafrost as carbon storage habitats, and developed a new goal, 'Aboriginal Needs', to measure access of Aboriginal people to traditional marine hunting and fishing grounds. Second, we evaluated various goal weighting schemes based on preferences elicited from the general public in online surveys. We quantified these public preferences in three ways: using Likert scores, simple ranks from a best-worst choice experiment, and model coefficients from the analysis of elicited choice experiment. The latter provided the clearest statistical discrimination among goals, and we recommend their use because they can more accurately reflect both public opinion and the trade-offs faced by policy-makers. This initial iteration of the COHI can be used as a baseline against which future COHI scores can be compared, and could potentially be used as a management tool to prioritise actions on a national scale and predict public support for these actions given that the goal weights are based on public priorities.
- Published
- 2017
37. Thrombocytopenia in neonatal sepsis: Incidence, severity and risk factors
- Author
-
Ree, Isabelle M. C., primary, Fustolo-Gunnink, Suzanne F., additional, Bekker, Vincent, additional, Fijnvandraat, Karin J., additional, Steggerda, Sylke J., additional, and Lopriore, Enrico, additional
- Published
- 2017
- Full Text
- View/download PDF
38. Incorporating public priorities in the Ocean Health Index: Canada as a case study
- Author
-
Daigle, Rémi M., primary, Archambault, Philippe, additional, Halpern, Benjamin S., additional, Stewart Lowndes, Julia S., additional, and Côté, Isabelle M., additional
- Published
- 2017
- Full Text
- View/download PDF
39. Dorsal Anterior Cingulate Thickness Is Related to Alexithymia in Childhood Trauma-Related PTSD
- Author
-
Scott L. Rauch, Lauren A. Demers, Isabelle M. Rosso, Elizabeth A. Olson, and David J. Crowley
- Subjects
Child abuse ,Adult ,Male ,medicine.medical_specialty ,lcsh:Medicine ,Poison control ,behavioral disciplines and activities ,Gyrus Cinguli ,Stress Disorders, Post-Traumatic ,03 medical and health sciences ,Toronto Alexithymia Scale ,Young Adult ,0302 clinical medicine ,5. Gender equality ,Alexithymia ,mental disorders ,medicine ,Humans ,Affective Symptoms ,Gray Matter ,lcsh:Science ,Psychiatry ,Anterior cingulate cortex ,Brain Mapping ,Multidisciplinary ,medicine.diagnostic_test ,business.industry ,Adult Survivors of Child Abuse ,lcsh:R ,Organ Size ,Middle Aged ,medicine.disease ,Magnetic Resonance Imaging ,030227 psychiatry ,Physical abuse ,medicine.anatomical_structure ,Sexual abuse ,lcsh:Q ,Female ,business ,030217 neurology & neurosurgery ,psychological phenomena and processes ,Psychopathology ,Research Article - Abstract
Alexithymia, or "no words for feelings", is highly prevalent in samples with childhood maltreatment and posttraumatic stress disorder (PTSD). The dorsal anterior cingulate cortex (dACC) has been identified as a key region involved in alexithymia, early life trauma, and PTSD. Functional alterations in the dACC also have been associated with alexithymia in PTSD. This study examined whether dACC morphology is a neural correlate of alexithymia in child maltreatment-related PTSD. Sixteen adults with PTSD and a history of childhood sexual abuse, physical abuse, or exposure to domestic violence, and 24 healthy controls (HC) completed the Toronto Alexithymia Scale 20 (TAS-20) and underwent magnetic resonance imaging. Cortical thickness of the dACC was measured using FreeSurfer, and values were correlated with TAS-20 scores, controlling for sex and age, in both groups. Average TAS-20 score was significantly higher in the PTSD than the HC group. TAS-20 scores were significantly positively associated with dACC thickness only in the PTSD group. This association was strongest in the left hemisphere and for TAS-20 subscales that assess difficulty identifying and describing feelings. We found that increasing dACC gray matter thickness is a neural correlate of greater alexithymia in the context of PTSD with childhood maltreatment. While findings are correlational, they motivate further inquiry into the relationships between childhood adversity, emotional awareness and expression, and dACC morphologic development in trauma-related psychopathology.
- Published
- 2015
40. Retrospective analysis of real-world data to evaluate actionability of a comprehensive molecular profiling panel in solid tumor tissue samples (REALM study).
- Author
-
Karen Leroy, Clarisse Audigier Valette, Jérôme Alexandre, Lise Boussemart, Jean Chiesa, Clotilde Deldycke, Carlos Gomez-Rocca, Antoine Hollebecque, Jacqueline Lehmann-Che, Antoinette Lemoine, Sandrine Mansard, Jacques Medioni, Isabelle Monnet, Samia Mourah, Thomas Pierret, Dominique Spaëth, Alexandre Civet, Sandrine Galoin, and Antoine Italiano
- Subjects
Medicine ,Science - Abstract
IntroductionConsidering the growing interest in matched cancer treatment, our aim was to evaluate the ability of a comprehensive genomic profiling (CGP) assay to propose at least one targeted therapy given an identified genomic alteration or signature (actionability), and to collect the treatment modifications based on the CGP test results in clinical practise for solid tumors.MethodsThis retrospective, multicentre French study was conducted among 25 centres that participated in a free of charge program between 2017 and 2019 for a tissue CGP test. Data were collected on the patient, disease, tumor genomic profile, treatment suggested in the report (related to the genomic profile results) and subsequent therapeutic decisions according to the physician's declaration.ResultsAmong the 416 patients, most had lung cancer (35.6%), followed by biliary tract cancer (11.5%) or rare cancers (11.1%); 75% had a metastatic disease. The actionability was 75.0% (95% CI [70.6%-78.9%]) for all patients, 85.1% and 78.4%, respectively in lung cancer and metastatic patients. After exclusion of clinical trial suggestions, the actionability decreased to 62.3% (95% CI [57.5%-66.8%]). Treatment modification based on the test results was observed in 17.3% of the patients and was more frequent in metastatic disease (OR = 2.73, 95% CI [1.31-5.71], p = 0.007). The main reasons for no treatment modification were poor general condition (33.2%) and stable disease or remission (30.2%). The genomic-directed treatment changes were performed mostly during the first six months after the CGP test, and interestingly a substantial part was observed from six to 24 months after the genomic profiling.ConclusionThis French study provides information on the real-life actionability of a CGP test based on tissue samples, and trends to confirm its utility in clinical practice across the course of the disease, in particularly for patients with lung cancer and/or advanced disease.
- Published
- 2023
- Full Text
- View/download PDF
41. Sensitivity of outcome instruments in a priori selected patient groups after traumatic brain injury: Results from the CENTER-TBI study.
- Author
-
Nicole von Steinbuechel, Katrin Rauen, Amra Covic, Ugne Krenz, Fabian Bockhop, Isabelle Mueller, Katrin Cunitz, Suzanne Polinder, Ewout W Steyerberg, Johannes Vester, Marina Zeldovich, and CENTER-TBI participants investigators
- Subjects
Medicine ,Science - Abstract
Traumatic brain injury (TBI) can negatively impact patients' lives on many dimensions. Multiple instruments are available for evaluating TBI outcomes, but it is still unclear which instruments are the most sensitive for that purpose. This study examines the sensitivity of nine outcome instruments in terms of their ability to discriminate within and between specific patient groups, selected a priori as identified from the literature, at three different time points within a year after TBI (i.e., 3, 6, and 12 months post injury). The sensitivity of the instruments to sociodemographic (sex, age, education), premorbid (psychological health status), and injury-related (clinical care pathways, TBI and extracranial injury severity) factors was assessed by means of cross-sectional multivariate Wei-Lachin analyses. The Glasgow Outcome Scale Extended (GOSE)-the standard in the field of TBI for measuring functional recovery-demonstrated the highest sensitivity in most group comparisons. However, as single functional scale, it may not be able to reflect the multidimensional nature of the outcome. Therefore, the GOSE was used as a reference for further sensitivity analyses on more specific outcome scales, addressing further potential deficits following TBI. The physical component summary score (PCS) of the generic health-related quality of life (HRQOL) instruments (SF-36v2/-12v2) and the TBI-specific HRQOL instruments (QOLIBRI/-OS) were most sensitive in distinguishing recovery after TBI across all time points and patient groups, followed by the RPQ assessing post-concussion symptoms and the PHQ-9 measuring depression. The SF-36v2/-12v2 mental component summary score and the GAD-7 measuring anxiety were less sensitive in several group comparisons. The assessment of the functional recovery status combined with generic HRQOL (the PCS of the SF-12v2), disease-specific HRQOL (QOLIBRI-OS), and post-concussion symptoms (RPQ) can provide a sensitive, comprehensive, yet time-efficient evaluation of the health status of individuals after TBI in different patient groups.
- Published
- 2023
- Full Text
- View/download PDF
42. Medico-economic comparison of two anticoagulant treatment strategies: Vitamin K antagonists vs. direct oral anticoagulants in older adults in nursing homes in France. The 'MIKADO' study.
- Author
-
George Pisica-Donose, Matthieu Piccoli, Bastien Genet, Stéphane Bouee, Stefan Berechet, Ion Berechet, Antonin Dacasa Cortes, Sabri Atsamena, Catherine Bayle, Mihai Badescu, François Catelain, Lynda Kermeche, Isabelle Merlier, Sahondranirina Rakotoniary, Valérie Savin, Ariane Vidal, Jean-Sébastien Vidal, and Olivier Hanon
- Subjects
Medicine ,Science - Abstract
ObjectivesCurrently, two classes of oral anticoagulants are available in nursing home residents: vitamin K antagonists (VKA) and direct oral anticoagulants (DOAC). DOACs have a higher net clinical benefit than VKAs but DOACs are about 10 times more expensive than VKAs. The objective of our study was to assess and compare the overall costs of anti-coagulant strategy (VKA or DOAC), i.e., including drugs, laboratory costs and time spent in human capital (nurses and medical time) in nursing homes in France.MethodsThis was an observational, multicenter, prospective study including nine nursing homes in France. Among these nursing homes, 241 patients aged 75 years and older and treated with VKA (n = 140) or DOAC (n = 101) therapy accepted to participate in the study.ResultsDuring the 3-month follow-up period, the adjusted mean costs per patient were higher for VKA than DOACs for nurse care (€327 (57) vs. €154 (56), pConclusionOur study showed that in nursing homes despite a higher drug cost, DOAC therapy is associated with a lower total cost and less time used by nurses and physicians for drug monitoring when compared to VKA.
- Published
- 2023
- Full Text
- View/download PDF
43. Effects of Protection and Sediment Stress on Coral Reefs in Saint Lucia
- Author
-
Bégin, Chantale, primary, Schelten, Christiane K., additional, Nugues, Maggy M., additional, Hawkins, Julie, additional, Roberts, Callum, additional, and Côté, Isabelle M., additional
- Published
- 2016
- Full Text
- View/download PDF
44. Differential Juvenile Hormone Variations in Scale Insect Extreme Sexual Dimorphism
- Author
-
Toshiharu Tanaka, Takahiro Shiotsuki, Chieka Minakuchi, Sayumi Tanaka, Akiya Jouraku, and Isabelle M. Vea
- Subjects
Male ,0301 basic medicine ,Physiology ,Oviposition ,Gene Expression ,lcsh:Medicine ,Genes, Insect ,Molting ,0302 clinical medicine ,Krüppel ,Reproductive Physiology ,Gene expression ,Medicine and Health Sciences ,lcsh:Science ,Receptor ,media_common ,Genetics ,Sex Characteristics ,Insect Metamorphosis ,Multidisciplinary ,Juvenile Hormones ,Insects ,Pupa ,Female ,Signal Transduction ,Research Article ,medicine.medical_specialty ,Arthropoda ,media_common.quotation_subject ,Kruppel-Like Transcription Factors ,Biology ,Hemiptera ,03 medical and health sciences ,Extraction techniques ,Internal medicine ,medicine ,Animals ,Metamorphosis ,Gene ,lcsh:R ,Organisms ,Genetic Variation ,Biology and Life Sciences ,Pupae ,Invertebrates ,Holometabolism ,RNA extraction ,Nymphs ,Research and analysis methods ,Sexual dimorphism ,030104 developmental biology ,Endocrinology ,Juvenile hormone ,lcsh:Q ,Transcriptome ,Zoology ,Entomology ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Scale insects have evolved extreme sexual dimorphism, as demonstrated by sedentary juvenile-like females and ephemeral winged males. This dimorphism is established during the post-embryonic development; however, the underlying regulatory mechanisms have not yet been examined. We herein assessed the role of juvenile hormone (JH) on the diverging developmental pathways occurring in the male and female Japanese mealybug Planococcus kraunhiae (Kuwana). We provide, for the first time, detailed gene expression profiles related to JH signaling in scale insects. Prior to adult emergence, the transcript levels of JH acid O-methyltransferase, encoding a rate-limiting enzyme in JH biosynthesis, were higher in males than in females, suggesting that JH levels are higher in males. Furthermore, male quiescent pupal-like stages were associated with higher transcript levels of the JH receptor gene, Methoprene-tolerant and its co-activator taiman, as well as the JH early-response genes, Krüppel homolog 1 and broad. The exposure of male juveniles to an ectopic JH mimic prolonged the expression of Krüppel homolog 1 and broad, and delayed adult emergence by producing a supernumeral pupal stage. We propose that male wing development is first induced by up-regulated JH signaling compared to female expression pattern, but a decrease at the end of the prepupal stage is necessary for adult emergence, as evidenced by the JH mimic treatments. Furthermore, wing development seems linked to JH titers as JHM treatments on the pupal stage led to wing deformation. The female pedomorphic appearance was not reflected by the maintenance of high levels of JH. The results in this study suggest that differential variations in JH signaling may be responsible for sex-specific and radically different modes of metamorphosis.
- Published
- 2016
45. Face Your Fears: Cleaning Gobies Inspect Predators despite Being Stressed by Them
- Author
-
Isabelle M. Côté, Marta C. Soares, Redouan Bshary, Sónia C. Cardoso, and Rui Filipe Oliveira
- Subjects
0106 biological sciences ,Coral reef fish ,Science ,Captivity ,Marine Biology ,Cleaner fish ,010603 evolutionary biology ,01 natural sciences ,Predation ,Fight-or-flight response ,Conflict, Psychological ,03 medical and health sciences ,Behavioral Ecology ,Animals ,Animal communication ,Conflict (Psychology) ,14. Life underwater ,Social Behavior ,Cortisol level ,Biology ,Physiological Ecology ,030304 developmental biology ,Animal Management ,Social stress ,0303 health sciences ,Evolutionary Biology ,Multidisciplinary ,biology ,Animal Behavior ,Ecology ,Behavior, Animal ,Marine Ecology ,Fishes ,Agriculture ,Fisheries Science ,biology.organism_classification ,Perciformes ,Animal Communication ,Predatory Behavior ,Medicine ,Veterinary Science ,Social psychology ,Zoology ,Stress, Psychological ,Research Article - Abstract
social stressors typically elicit two distinct behavioural responses in vertebrates: an active response (i.e., "fight or flight") or behavioural inhibition (i.e., freezing). Here, we report an interesting exception to this dichotomy in a Caribbean cleaner fish, which interacts with a wide variety of reef fish clients, including predatory species. Cleaning gobies appraise predatory clients as potential threat and become stressed in their presence, as evidenced by their higher cortisol levels when exposed to predatory rather than to non-predatory clients. Nevertheless, cleaning gobies neither flee nor freeze in response to dangerous clients but instead approach predators faster (both in captivity and in the wild), and interact longer with these clients than with non-predatory clients (in the wild). We hypothesise that cleaners interrupt the potentially harmful physiological consequences elicited by predatory clients by becoming increasingly proactive and by reducing the time elapsed between client approach and the start of the interaction process. The activation of a stress response may therefore also be responsible for the longer cleaning service provided by these cleaners to predatory clients in the wild. Future experimental studies may reveal similar patterns in other social vertebrate species when, for instance, individuals approach an opponent for reconciliation after a conflict. Fundação para a Ciência e Tecnologia grant: (PTDC/MAR/105276/2008); Natural Science and Engineering Research Council of Canada; Swiss Science Foundation.
- Published
- 2012
46. Selective Regulation of NR2B by Protein Phosphatase-1 for the Control of the NMDA Receptor in Neuroprotection
- Author
-
Fabrice Heitz, Shiva K. Tyagarajan, Isabelle M. Mansuy, Mélissa Farinelli, Fritjof Helmchen, Benjamin F. Grewe, and University of Zurich
- Subjects
Transcription, Genetic ,lcsh:Medicine ,Hippocampus ,Mice ,0302 clinical medicine ,Protein Phosphatase 1 ,Molecular Cell Biology ,Phosphorylation ,lcsh:Science ,0303 health sciences ,Multidisciplinary ,Neurodegeneration ,Glutamate receptor ,Neurodegenerative Diseases ,Animal Models ,3. Good health ,Cell biology ,NMDA receptor ,Signal transduction ,Research Article ,Signal Transduction ,Phosphatase ,Neurophysiology ,610 Medicine & health ,Neuroimaging ,Mice, Transgenic ,1100 General Agricultural and Biological Sciences ,Biology ,Neuroprotection ,Receptors, N-Methyl-D-Aspartate ,Gene Expression Regulation, Enzymologic ,Dephosphorylation ,03 medical and health sciences ,Model Organisms ,1300 General Biochemistry, Genetics and Molecular Biology ,medicine ,Animals ,Humans ,030304 developmental biology ,1000 Multidisciplinary ,10242 Brain Research Institute ,lcsh:R ,Protein phosphatase 1 ,medicine.disease ,Mice, Inbred C57BL ,Oxygen ,Glucose ,nervous system ,Cellular Neuroscience ,570 Life sciences ,biology ,lcsh:Q ,Calcium ,Molecular Neuroscience ,Calcium-Calmodulin-Dependent Protein Kinase Type 2 ,030217 neurology & neurosurgery ,Neuroscience - Abstract
An imbalance between pro-survival and pro-death pathways in brain cells can lead to neuronal cell death and neurodegeneration. While such imbalance is known to be associated with alterations in glutamatergic and Ca2+ signaling, the underlying mechanisms remain undefined. We identified the protein Ser/Thr phosphatase protein phosphatase-1 (PP1), an enzyme associated with glutamate receptors, as a key trigger of survival pathways that can prevent neuronal death and neurodegeneration in the adult hippocampus. We show that PP1α overexpression in hippocampal neurons limits NMDA receptor overactivation and Ca2+ overload during an excitotoxic event, while PP1 inhibition favors Ca2+ overload and cell death. The protective effect of PP1 is associated with a selective dephosphorylation on a residue phosphorylated by CaMKIIα on the NMDA receptor subunit NR2B, which promotes pro-survival pathways and associated transcriptional programs. These results reveal a novel contributor to the mechanisms of neuroprotection and underscore the importance of PP1-dependent dephosphorylation in these mechanisms. They provide a new target for the development of potential therapeutic treatment of neurodegeneration. ISSN:1932-6203
- Published
- 2012
47. Genome characterization of the oleaginous fungus Mortierella alpina
- Author
-
Peng Du, Yanlin Yang, Yan Ren, Yong Q. Chen, Wang Na, Wei Chen, Wei Wang, Suriguga Wang, Zhennan Gu, Lu Feng, Isabelle M. Berquin, Yun Feng, Lei Wang, Colin Ratledge, James S. Norris, Junguo Shen, Yuanda Song, Zhang Baixi, Hongchao Wang, Huanxin Zhang, Jiansheng Wu, Michael J. Thomas, Haiqin Chen, Xiang Liu, Yang Li, and Hao Zhang
- Subjects
Applied Microbiology ,lcsh:Medicine ,Genome ,Biochemistry ,chemistry.chemical_compound ,Mortierella ,Gene Duplication ,Genome Sequencing ,lcsh:Science ,Phylogeny ,Genetics ,0303 health sciences ,Multidisciplinary ,Ascomycota ,biology ,Fatty Acids ,Fungal genetics ,Chromosome Mapping ,Genomics ,Lipids ,Multigene Family ,Lipogenesis ,Arachidonic acid ,lipids (amino acids, peptides, and proteins) ,Genome, Fungal ,Research Article ,Mycology ,Microbiology ,Fungal Proteins ,Molecular Genetics ,03 medical and health sciences ,Industrial Microbiology ,Reproduction, Asexual ,Biology ,Fatty acid synthesis ,030304 developmental biology ,Microbial Metabolism ,Staining and Labeling ,030306 microbiology ,lcsh:R ,Fungi ,Computational Biology ,Lipid metabolism ,biology.organism_classification ,Lipid Metabolism ,Protein Structure, Tertiary ,chemistry ,Genome, Mitochondrial ,lcsh:Q - Abstract
Mortierella alpina is an oleaginous fungus which can produce lipids accounting for up to 50% of its dry weight in the form of triacylglycerols. It is used commercially for the production of arachidonic acid. Using a combination of high throughput sequencing and lipid profiling, we have assembled the M. alpina genome, mapped its lipogenesis pathway and determined its major lipid species. The 38.38 Mb M. alpina genome shows a high degree of gene duplications. Approximately 50% of its 12,796 gene models, and 60% of genes in the predicted lipogenesis pathway, belong to multigene families. Notably, M. alpina has 18 lipase genes, of which 11 contain the class 2 lipase domain and may share a similar function. M. alpina's fatty acid synthase is a single polypeptide containing all of the catalytic domains required for fatty acid synthesis from acetyl-CoA and malonyl-CoA, whereas in many fungi this enzyme is comprised of two polypeptides. Major lipids were profiled to confirm the products predicted in the lipogenesis pathway. M. alpina produces a complex mixture of glycerolipids, glycerophospholipids and sphingolipids. In contrast, only two major sterol lipids, desmosterol and 24(28)-methylene-cholesterol, were detected. Phylogenetic analysis based on genes involved in lipid metabolism suggests that oleaginous fungi may have acquired their lipogenic capacity during evolution after the divergence of Ascomycota, Basidiomycota, Chytridiomycota and Mucoromycota. Our study provides the first draft genome and comprehensive lipid profile for M. alpina, and lays the foundation for possible genetic engineering of M. alpina to produce higher levels and diverse contents of dietary lipids.
- Published
- 2011
48. Searching for speciation genes: molecular evidence for selection associated with colour morphotypes in the Caribbean reef fish genus Hypoplectrus
- Author
-
Isabelle M. Côté, Ben G. Holt, Brent C. Emerson, and Natural Environment Research Council (UK)
- Subjects
Genetic Markers ,Coral reefs ,Sexual Selection ,Evolutionary Processes ,Heredity ,Coral reef fish ,Genetic Speciation ,Speciation ,Allopatric speciation ,Zoology ,lcsh:Medicine ,Evolutionary Selection ,Morphotypes ,Forms of Evolution ,Divergent Evolution ,Genetic ,Genus ,Phenotypic divergence ,Natural Selection ,Genetics ,Animals ,Geograhy ,Amplified Fragment Length Polymorphism Analysis ,Selection, Genetic ,lcsh:Science ,Biology ,Evolutionary Biology ,Multidisciplinary ,biology ,Population Biology ,Pigmentation ,lcsh:R ,Computational Biology ,biology.organism_classification ,Genetic divergence ,Phenotypes ,Phenotype ,Hypoplectrus ,Genetic marker ,Genetic Loci ,Evolutionary Ecology ,Genetic Polymorphism ,Amplified fragment length polymorphism ,lcsh:Q ,Bass ,Population Genetics ,Research Article - Abstract
Closely related species that show clear phenotypic divergence, but without obvious geographic barriers, can provide opportunities to study how diversification can occur when opportunities for allopatric speciation are limited. We examined genetic divergence in the coral reef fish genus Hypoplectrus (family: Serranidae), which comprises of 10-14 morphotypes that are distinguished solely by their distinct colour patterns, but which show little genetic differentiation. Our goal was to detect loci that show clear disequilibrium between morphotypes and across geographical locations. We conducted Amplified Fragment Length Polymorphism molecular analysis to quantify genetic differentiation among, and selection between, morphotypes. Three loci were consistently divergent beyond neutral expectations in repeated pair-wise morphotype comparisons using two different methods. These loci provide the first evidence for genes that may be associated with colour morphotype in the genus Hypoplectrus., This work was funded by the National Environment Research Council (studentship S NER/SJ2004/13064 and LSMSF application no. EK76-02/05), with the additional funding by SMGF and the Sydney L. Wright Fellowship (contribution number 1700).
- Published
- 2011
49. Dorsal Anterior Cingulate Thickness Is Related to Alexithymia in Childhood Trauma-Related PTSD
- Author
-
Demers, Lauren A., primary, Olson, Elizabeth A., additional, Crowley, David J., additional, Rauch, Scott L., additional, and Rosso, Isabelle M., additional
- Published
- 2015
- Full Text
- View/download PDF
50. Simplification of Caribbean Reef-Fish Assemblages over Decades of Coral Reef Degradation
- Author
-
Alvarez-Filip, Lorenzo, primary, Paddack, Michelle J., additional, Collen, Ben, additional, Robertson, D. Ross, additional, and Côté, Isabelle M., additional
- Published
- 2015
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.