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1. PLA2R antibody, PLA2R rs4664308 polymorphism and PLA2R mRNA levels in Tunisian patients with primary membranous nephritis.

Author

Tarak Dhaouadi, Jihen Abdellatif, Raja Trabelsi, Hanene Gaied, Sameh Chamkhi, Imen Sfar, Rym Goucha, Fethi Ben Hamida, Taieb Ben Abdallah, and Yousr Gorgi

Subjects
Medicine, Science
Abstract

BackgroundPrimary membranous nephritis (PMN) is an autoimmune disease induced by the deposit of antibodies (Ab) to the phospholipase receptor A2 receptor (PLA2R) on podocytes. In this context, we aimed to assess the relationships between anti-PLA2R Ab, PLA2R rs4664308 SNP, PLA2R mRNA levels and PMN susceptibility and outcome.MethodsSixty-eight PMN patients, 30 systemic lupus erythematosus (SLE) patients with secondary MN and 30 healthy control subjects served for anti-PLA2R Ab measurement by ELISA and PLA2R rs4664308 SNP genotyping by a commercial real-time PCR. Twenty patients with tubulo-interstitial nephritis (TIN) were used as controls for renal PLA2R mRNA quantification in PMN patients from kidney biopsies. PLA2R mRNA quantification was carried-out by real-time PCR after RNA extraction.ResultsForty-three (63.2%) PMN patients received initial therapy consisting of alternating monthly cycles of corticosteroids and cyclophosphamide. Twelve (17.6%) patients had resistant PMN to initial therapy and were consecutively treated by cyclosporine or tacrolimus. Anti-PLA2R Ab were positive in 54 (79.4%) PMN patients, while all SLE patients and controls were negative, pConclusionAnti-PLA2R Ab and renal PLA2R mRNA could be useful markers for PMN outcome predicting. The PLA2R rs6446308 SNP is associated with PMN susceptibility in Tunisians.

Published
2020
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2. IL-17A, IL-17RC polymorphisms and IL17 plasma levels in Tunisian patients with rheumatoid arthritis.

Author

Tarak Dhaouadi, Mayssa Chahbi, Youssra Haouami, Imen Sfar, Leila Abdelmoula, Taieb Ben Abdallah, and Yousr Gorgi

Subjects
Medicine, Science
Abstract

Interleukin-17 (IL-17), a cytokine mainly secreted by Th17 cells, seems to play a significant role in the pathogenesis of rheumatoid arthritis (RA). Functional genetic polymorphisms in IL-17 and its receptor genes can influence either qualitatively or quantitatively their functions. Therefore, we aimed to study the impact of IL17-A and IL17RC polymorphisms on plasma level of IL-17 and RA susceptibility and severity.In this context, IL-17A*rs2275913 and IL-17RC*rs708567 polymorphisms were investigated together with the quantification of IL17 plasma level in 115 RA patients and 91 healthy control subjects matched in age, sex and ethnic origin.There were no statistically significant associations between IL-17A and IL-17RC studied polymorphisms and RA susceptibility. In contrast, IL-17A plasma levels were significantly higher in patients (55.07 pg/ml) comparatively to controls (4.75 pg/ml), p

Published
2018
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3. PLA2R antibody, PLA2R rs4664308 polymorphism and PLA2R mRNA levels in Tunisian patients with primary membranous nephritis

Author

Jihen Abdellatif, Imen Sfar, Sameh Chamkhi, Taieb Ben Abdallah, Rym Goucha, Yousr Gorgi, Tarak Dhaouadi, Fethi Ben Hamida, Raja Trabelsi, and Hanene Gaied

Subjects
Male, Biopsy, Single Nucleotide Polymorphisms, 030232 urology & nephrology, 030204 cardiovascular system & hematology, Kidney, Biochemistry, Glomerulonephritis, Membranous, Gastroenterology, Steroid Therapy, chemistry.chemical_compound, 0302 clinical medicine, Medicine and Health Sciences, Nephritis, Multidisciplinary, Proteinuria, medicine.diagnostic_test, Pharmaceutics, Glomerulonephritis, Middle Aged, medicine.anatomical_structure, Nephrology, Creatinine, Medicine, Female, Anatomy, medicine.symptom, Research Article, medicine.drug, medicine.medical_specialty, Tunisia, Cyclophosphamide, Science, Corticosteroid Therapy, Immunology, Surgical and Invasive Medical Procedures, Systemic Lupus Erythematosus, Polymorphism, Single Nucleotide, Autoimmune Diseases, 03 medical and health sciences, Signs and Symptoms, Rheumatology, Drug Therapy, Internal medicine, Genetics, medicine, Humans, Genetic Predisposition to Disease, RNA, Messenger, Autoantibodies, Autoimmune disease, Lupus Erythematosus, business.industry, Receptors, Phospholipase A2, Biology and Life Sciences, Kidneys, Renal System, medicine.disease, chemistry, Case-Control Studies, Clinical Immunology, Clinical Medicine, business, Biomarkers
Abstract

BackgroundPrimary membranous nephritis (PMN) is an autoimmune disease induced by the deposit of antibodies (Ab) to the phospholipase receptor A2 receptor (PLA2R) on podocytes. In this context, we aimed to assess the relationships between anti-PLA2R Ab, PLA2R rs4664308 SNP, PLA2R mRNA levels and PMN susceptibility and outcome.MethodsSixty-eight PMN patients, 30 systemic lupus erythematosus (SLE) patients with secondary MN and 30 healthy control subjects served for anti-PLA2R Ab measurement by ELISA and PLA2R rs4664308 SNP genotyping by a commercial real-time PCR. Twenty patients with tubulo-interstitial nephritis (TIN) were used as controls for renal PLA2R mRNA quantification in PMN patients from kidney biopsies. PLA2R mRNA quantification was carried-out by real-time PCR after RNA extraction.ResultsForty-three (63.2%) PMN patients received initial therapy consisting of alternating monthly cycles of corticosteroids and cyclophosphamide. Twelve (17.6%) patients had resistant PMN to initial therapy and were consecutively treated by cyclosporine or tacrolimus. Anti-PLA2R Ab were positive in 54 (79.4%) PMN patients, while all SLE patients and controls were negative, pConclusionAnti-PLA2R Ab and renal PLA2R mRNA could be useful markers for PMN outcome predicting. The PLA2R rs6446308 SNP is associated with PMN susceptibility in Tunisians.

Published
2020

4. IL-17A, IL-17RC polymorphisms and IL17 plasma levels in Tunisian patients with rheumatoid arthritis

Author

Imen Sfar, Youssra Haouami, Taieb Ben Abdallah, Leila Abdelmoula, Mayssa Chahbi, Tarak Dhaouadi, and Yousr Gorgi

Subjects
Male, 0301 basic medicine, Heredity, Physiology, medicine.medical_treatment, lcsh:Medicine, Arthritis, Pathology and Laboratory Medicine, Severity of Illness Index, Arthritis, Rheumatoid, Pathogenesis, Mathematical and Statistical Techniques, 0302 clinical medicine, Immune Physiology, Blood plasma, Medicine and Health Sciences, lcsh:Science, Innate Immune System, Multidisciplinary, biology, Interleukin-17, Animal Models, Middle Aged, Body Fluids, Genetic Mapping, Blood, Cytokine, Experimental Organism Systems, Area Under Curve, Rheumatoid arthritis, Physical Sciences, Cytokines, Female, Disease Susceptibility, Interleukin 17, Anatomy, Antibody, Statistics (Mathematics), Research Article, Adult, medicine.medical_specialty, Tunisia, Genotype, Immunology, Black People, Rheumatoid Arthritis, Variant Genotypes, Mouse Models, Research and Analysis Methods, Polymorphism, Single Nucleotide, Blood Plasma, Autoimmune Diseases, 03 medical and health sciences, Model Organisms, Signs and Symptoms, Rheumatology, Diagnostic Medicine, Internal medicine, Genetics, medicine, Humans, Statistical Methods, Alleles, 030203 arthritis & rheumatology, business.industry, lcsh:R, Case-control study, Biology and Life Sciences, Molecular Development, medicine.disease, 030104 developmental biology, Endocrinology, ROC Curve, Immune System, Case-Control Studies, Lesions, biology.protein, Clinical Immunology, lcsh:Q, Clinical Medicine, business, Mathematics, Developmental Biology, Meta-Analysis
Abstract

Background Interleukin-17 (IL-17), a cytokine mainly secreted by Th17 cells, seems to play a significant role in the pathogenesis of rheumatoid arthritis (RA). Functional genetic polymorphisms in IL-17 and its receptor genes can influence either qualitatively or quantitatively their functions. Therefore, we aimed to study the impact of IL17-A and IL17RC polymorphisms on plasma level of IL-17 and RA susceptibility and severity. Methods In this context, IL-17A*rs2275913 and IL-17RC*rs708567 polymorphisms were investigated together with the quantification of IL17 plasma level in 115 RA patients and 91 healthy control subjects matched in age, sex and ethnic origin. Results There were no statistically significant associations between IL-17A and IL-17RC studied polymorphisms and RA susceptibility. In contrast, IL-17A plasma levels were significantly higher in patients (55.07 pg/ml) comparatively to controls (4.75 pg/ml), p

Published
2018

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