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1. Airway dendritic cell maturation in children exposed to air pollution.

Author

Abigail L Whitehouse, Naseem Mushtaq, Lisa Miyashita, Benjamin Barratt, Ameerah Khan, Harpal Kalsi, Lee Koh, Michele G Padovan, Rossa Brugha, Frances R Balkwill, Andrew J Stagg, and Jonathan Grigg

Subjects
Medicine, Science
Abstract

Urban particulate matter (PM) enhances airway dendritic cell (DC) maturation in vitro. However, to date, there are no data on the association between exposure to urban PM and DC maturation in vivo. We sought to determine whether exposure of school-age children (8 to 14 y) to PM was associated with expression of CD86, a marker of maturation of airway conventional DCs (cDC). Healthy London school children underwent spirometry and sputum induction. Flow cytometry was used to identify CD86 and CCR7 expression on cDC subsets (CD1c+ cDC2 and CD141+ cDC1). Tertiles of mean annual exposure to PM ≤ 10 microns (PM10) at the school address were determined using the London Air Quality Toolkit model. Tertiles of exposure from the 409 children from 19 schools recruited were; lower (23.1 to 25.6 μg/m3, n = 138), middle (25.6 to 26.8 μg/m3, n = 126), and upper (26.8 to 31.0 μg/m3, n = 145). DC expression was assessed in 164/370 (44%) children who completed sputum induction. The proportion (%) of cDC expressing CD86 in the lower exposure tertile (n = 47) was lower compared with the upper exposure tertile (n = 49); (52% (44 to 70%) vs 66% (51 to 82%), p

Published
2020
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2. Airway dendritic cell maturation in children exposed to air pollution

Author

Frances R. Balkwill, Andrew J. Stagg, Rossa Brugha, Jonathan Grigg, Ameerah Khan, Harpal Kalsi, Benjamin Barratt, Naseem Mushtaq, Michele Padovan, Abigail Whitehouse, Lisa Miyashita, and Lee Koh

Subjects
Male, Sputum Cytology, Physiology, Social Sciences, Families, Spectrum Analysis Techniques, Sociology, Cellular types, London, Medicine, Child, Children, Air Pollutants, Schools, Multidisciplinary, medicine.diagnostic_test, Immune cells, Environmental exposure, Flow Cytometry, Pollution, Body Fluids, Spectrophotometry, White blood cells, Female, Cytophotometry, Anatomy, medicine.symptom, Research Article, Spirometry, Cell biology, Blood cells, Adolescent, Science, Immunology, T cells, Antigen-Presenting Cells, Cytotoxic T cells, Research and Analysis Methods, Education, Andrology, Air Pollution, Surface marker, Humans, Dc maturation, Medicine and health sciences, Biology and life sciences, business.industry, Ecology and Environmental Sciences, Sputum, Urban Health, Dendritic Cells, Environmental Exposure, Dendritic cell, Mucus, Animal cells, Age Groups, People and Places, Population Groupings, Particulate Matter, B7-2 Antigen, business, Airway
Abstract

Urban particulate matter (PM) enhances airway dendritic cell (DC) maturation in vitro. However, to date, there are no data on the association between exposure to urban PM and DC maturation in vivo. We sought to determine whether exposure of school-age children (8 to 14 y) to PM was associated with expression of CD86, a marker of maturation of airway conventional DCs (cDC). Healthy London school children underwent spirometry and sputum induction. Flow cytometry was used to identify CD86 and CCR7 expression on cDC subsets (CD1c+ cDC2 and CD141+ cDC1). Tertiles of mean annual exposure to PM ≤ 10 microns (PM10) at the school address were determined using the London Air Quality Toolkit model. Tertiles of exposure from the 409 children from 19 schools recruited were; lower (23.1 to 25.6 μg/m3, n = 138), middle (25.6 to 26.8 μg/m3, n = 126), and upper (26.8 to 31.0 μg/m3, n = 145). DC expression was assessed in 164/370 (44%) children who completed sputum induction. The proportion (%) of cDC expressing CD86 in the lower exposure tertile (n = 47) was lower compared with the upper exposure tertile (n = 49); (52% (44 to 70%) vs 66% (51 to 82%), p

Published
2020

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