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7 results on '"Garcia, Manuel"'

1. Understanding uptake of COVID-19 testing, vaccination, and boosters among Spanish-speaking Latines in the United States: Qualitative insights from Spanish speakers and key informants

Author

Tanner, Amanda E., primary, Hall, Mark A., additional, Aguilar-Palma, Sandy K., additional, Mann-Jackson, Lilli, additional, Alonzo, Jorge, additional, Bertoni, Alain G., additional, McCoy, Thomas P., additional, Garcia, Manuel, additional, Sucaldito, Ana D., additional, Turner, Mari Jo, additional, Robles Arvizu, Jose, additional, Russell, Laurie P., additional, and Rhodes, Scott D., additional

Published
2024
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2. Genomic and Metabolic Diversity of Marine Group I Thaumarchaeota in the Mesopelagic of Two Subtropical Gyres

Author

Swan, Brandon K, Chaffin, Mark D, Martinez-Garcia, Manuel, Morrison, Hilary G, Field, Erin K, Poulton, Nicole J, Masland, E Dashiell P, Harris, Christopher C, Sczyrba, Alexander, Chain, Patrick SG, Koren, Sergey, Woyke, Tanja, and Stepanauskas, Ramunas

Subjects
Microbiology, Oceanography, Biological Sciences, Earth Sciences, Biotechnology, Human Genome, Genetics, Life Below Water, Archaea, DNA, Archaeal, Phylogeny, General Science & Technology
Abstract

Marine Group I (MGI) Thaumarchaeota are one of the most abundant and cosmopolitan chemoautotrophs within the global dark ocean. To date, no representatives of this archaeal group retrieved from the dark ocean have been successfully cultured. We used single cell genomics to investigate the genomic and metabolic diversity of thaumarchaea within the mesopelagic of the subtropical North Pacific and South Atlantic Ocean. Phylogenetic and metagenomic recruitment analysis revealed that MGI single amplified genomes (SAGs) are genetically and biogeographically distinct from existing thaumarchaea cultures obtained from surface waters. Confirming prior studies, we found genes encoding proteins for aerobic ammonia oxidation and the hydrolysis of urea, which may be used for energy production, as well as genes involved in 3-hydroxypropionate/4-hydroxybutyrate and oxidative tricarboxylic acid pathways. A large proportion of protein sequences identified in MGI SAGs were absent in the marine cultures Cenarchaeum symbiosum and Nitrosopumilus maritimus, thus expanding the predicted protein space for this archaeal group. Identifiable genes located on genomic islands with low metagenome recruitment capacity were enriched in cellular defense functions, likely in response to viral infections or grazing. We show that MGI Thaumarchaeota in the dark ocean may have more flexibility in potential energy sources and adaptations to biotic interactions than the existing, surface-ocean cultures.

Published
2014

6. Capturing Single Cell Genomes of Active Polysaccharide Degraders: An Unexpected Contribution of Verrucomicrobia

Author

Martinez-Garcia, Manuel, primary, Brazel, David M., additional, Swan, Brandon K., additional, Arnosti, Carol, additional, Chain, Patrick S. G., additional, Reitenga, Krista G., additional, Xie, Gary, additional, Poulton, Nicole J., additional, Gomez, Monica Lluesma, additional, Masland, Dashiell E. D., additional, Thompson, Brian, additional, Bellows, Wendy K., additional, Ziervogel, Kai, additional, Lo, Chien-Chi, additional, Ahmed, Sanaa, additional, Gleasner, Cheryl D., additional, Detter, Chris J., additional, and Stepanauskas, Ramunas, additional

Published
2012
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