8 results on '"El-Shemy, Hany A."'
Search Results
2. Target-similarity search using Plasmodium falciparum proteome identifies approved drugs with anti-malarial activity and their possible targets
- Author
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Mogire, Reagan M., primary, Akala, Hoseah M., additional, Macharia, Rosaline W., additional, Juma, Dennis W., additional, Cheruiyot, Agnes C., additional, Andagalu, Ben, additional, Brown, Mathew L., additional, El-Shemy, Hany A., additional, and Nyanjom, Steven G., additional
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- 2017
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3. Mapping of QTL for resistance against the crucifer specialist herbivore Pieris brassicae in a new Arabidopsis inbred line population, Da(1)-12 x Ei-2
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Pfalz, Marina, Vogel, Heiko, Mitchell-Olds, Thomas, Kroymann, Juergen, El-Shemy, Hany, Ecologie Systématique et Evolution (ESE), Université Paris-Sud - Paris 11 (UP11)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Max Planck Institute for Chemical Ecology, Max-Planck-Gesellschaft, Department of Biology, and Duke University [Durham]
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0106 biological sciences ,Glycoside Hydrolases ,[SDV]Life Sciences [q-bio] ,Glucosinolates ,Quantitative Trait Loci ,Population ,Arabidopsis ,lcsh:Medicine ,Brassica ,Genetics and Genomics/Complex Traits ,Quantitative trait locus ,01 natural sciences ,Chromosomes, Plant ,Plant Biology/Plant Genetics and Gene Expression ,03 medical and health sciences ,Botany ,Animals ,Arabidopsis thaliana ,education ,lcsh:Science ,ComputingMilieux_MISCELLANEOUS ,Plant Diseases ,030304 developmental biology ,0303 health sciences ,Pieris brassicae ,education.field_of_study ,[SDV.GEN]Life Sciences [q-bio]/Genetics ,Multidisciplinary ,biology ,Myrosinase ,fungi ,lcsh:R ,Chromosome Mapping ,Plutella ,food and beverages ,Epistasis, Genetic ,biology.organism_classification ,Lepidoptera ,Crucifer ,Pieris (butterfly) ,lcsh:Q ,Research Article ,Plant Biology/Plant-Biotic Interactions ,010606 plant biology & botany - Abstract
Background In Arabidopsis thaliana and other crucifers, the glucosinolate-myrosinase system contributes to resistance against herbivory by generalist insects. As yet, it is unclear how crucifers defend themselves against crucifer-specialist insect herbivores. Methodology/Principal Findings We analyzed natural variation for resistance against two crucifer specialist lepidopteran herbivores, Pieris brassicae and Plutella xylostella, among Arabidopsis thaliana accessions and in a new Arabidopsis recombinant inbred line (RIL) population generated from the parental accessions Da(1)-12 and Ei-2. This RIL population consists of 201 individual F8 lines genotyped with 84 PCR-based markers. We identified six QTL for resistance against Pieris herbivory, but found only one weak QTL for Plutella resistance. To elucidate potential factors causing these resistance QTL, we investigated leaf hair (trichome) density, glucosinolates and myrosinase activity, traits known to influence herbivory by generalist insects. We identified several previously unknown QTL for these traits, some of which display a complex pattern of epistatic interactions. Conclusions/Significance Although some trichome, glucosinolate or myrosinase QTL co-localize with Pieris QTL, none of these traits explained the resistance QTL convincingly, indicating that resistance against specialist insect herbivores is influenced by other traits than resistance against generalists.
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- 2007
4. Allelopathic Effects of Water Hyacinth [Eichhornia crassipes]
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Shanab, Sanaa M. M., primary, Shalaby, Emad A., additional, Lightfoot, David A., additional, and El-Shemy, Hany A., additional
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- 2010
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5. Willow Leaves' Extracts Contain Anti-Tumor Agents Effective against Three Cell Types
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El-Shemy, Hany A., primary, Aboul-Enein, Ahmed M., additional, Aboul-Enein, Khalid Mostafa, additional, and Fujita, Kounosuke, additional
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- 2007
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6. A Candidate-Gene Association Study for Berry Colour and Anthocyanin Content in Vitis vinifera L.
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Cardoso, Silvana, Lau, Winston, Dias, José Eiras, Fevereiro, Pedro, Maniatis, Nikolas, and El-Shemy, Hany A.
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ANTHOCYANINS ,GENES ,GRAPES ,GENETIC research ,GLUCOSIDES ,BIOSYNTHESIS - Abstract
Anthocyanin content is a trait of major interest in Vitis vinifera L. These compounds affect grape and wine quality, and have beneficial effects on human health. A candidate-gene approach was used to identify genetic variants associated with anthocyanin content in grape berries. A total of 445 polymorphisms were identified in 5 genes encoding transcription factors and 10 genes involved in either the biosynthetic pathway or transport of anthocyanins. A total of 124 SNPs were selected to examine association with a wide range of phenotypes based on RP-HPLC analysis and visual characterization. The phenotypes were total skin anthocyanin (TSA) concentration but also specific types of anthocyanins and relative abundance. The visual assessment was based on OIV (Organisation Internationale de la Vigne et du Vin) descriptors for berry and skin colour. The genes encoding the transcription factors MYB11, MYBCC and MYCB were significantly associated with TSA concentration. UFGT and MRP were associated with several different types of anthocyanins. Skin and pulp colour were associated with nine genes (MYB11, MYBCC, MYC
B , UFGT, MRP, DFR, LDOX, CHI and GST). Pulp colour was associated with a similar group of 11 genes (MYB11, MYBCC, MYCB , MYCA , UFGT, MRP, GST, DFR, LDOX, CHI and CHSA ). Statistical interactions were observed between SNPs within the transcription factors MYB11, MYBCC and MYCB . SNPS within LDOX interacted with MYB11 and MYCB , while SNPs within CHI interacted with MYB11 only. Together, these findings suggest the involvement of these genes in anthocyanin content and on the regulation of anthocyanin biosynthesis. This work forms a benchmark for replication and functional studies. [ABSTRACT FROM AUTHOR]- Published
- 2012
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7. Reduction of Soybean Meal Non-Starch Polysaccharides and α-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments.
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Opazo, Rafael, Ortúzar, Felipe, Navarrete, Paola, Espejo, Romilio, Romero, Jaime, and El-Shemy, Hany A.
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SOYBEAN meal ,FEED research ,POLYSACCHARIDES ,GALACTOSIDES ,CELLULOLYTIC bacteria ,SOLID-state fermentation - Abstract
Soybean meal (SBM) is an important protein source in animal feed. However, the levels of SBM inclusion are restricted in some animal species by the presence of antinutritional factors (ANFs), including non-starch polysaccharides (NSPs) and a- galactosides (GOSs). The aim of this study was to reduce the soybean meal NSPs and GOSs by solid-state fermentation (SSF) using a combination of cellulolytic bacteria isolated from different environments (termites, earthworms, corn silage and bovine ruminal content). To analyse the key enzymatic activities, the isolates were grown in minimal media containing NSPs extracted from SBM. The selected bacterial strains belonged to the genera Streptomyces, Cohnella and Cellulosimicrobium. SSF resulted in a reduction of nearly 24% in the total NSPs, 83% of stachyose and 69% of raffinose and an increase in the protein content. These results suggest that cellulolytic bacteria-based SSF processing facilitates SBM nutritional improvement. In addition, the use of fermented SBM in animal diets can be recommended. [ABSTRACT FROM AUTHOR]
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- 2012
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8. Modifying ligand-induced and constitutive signaling of the human 5-HT4 receptor
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Trieu Nguyen, Sylvie Claeysen, Wei Chun Chang, Edward C. Hsiao, Jennifer K. Ng, Lucie P. Pellissier, Bruce R. Conklin, Gladstone Institute of Cardiovascular Disease, University of California [San Francisco] (UCSF), University of California-University of California, Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), This study was supported by NIH grant HL-60664 and American Heart Association Predoctoral Fellowship 0415005Y (to WCC), NIH Fellowship Training Grant 2T32DK07418-26 (to ECH), J David Gladstone CIRM Fellowship (to ECH), and Kirschstein NRSA Fellowship F32A624044-02 (to JKN)., and El-Shemy, Hany
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lcsh:Medicine ,5-HT4 ,ligand ,Ligands ,Cell Biology/Cell Signaling ,5-HT7 receptor ,Mice ,0302 clinical medicine ,Complementary ,Receptors ,Cyclic AMP ,lcsh:Science ,Calcium signaling ,0303 health sciences ,Multidisciplinary ,Cell biology ,Electroporation ,Biochemistry ,Signal transduction ,récepteur ,Autre (Sciences du Vivant) ,Signal Transduction ,Research Article ,Serotonin ,DNA, Complementary ,General Science & Technology ,G protein ,1.1 Normal biological development and functioning ,Enzyme-Linked Immunosorbent Assay ,Biology ,Cell Line ,03 medical and health sciences ,GTP-binding protein regulators ,Clinical Research ,Underpinning research ,GTP-Binding Proteins ,Animals ,Humans ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Autocrine signalling ,5-HT receptor ,DNA Primers ,030304 developmental biology ,G protein-coupled receptor ,Pharmacology ,Base Sequence ,lcsh:R ,DNA ,protéine gpcr ,voie de signalisation ,Mutation ,lcsh:Q ,Generic health relevance ,Receptors, Serotonin, 5-HT4 ,030217 neurology & neurosurgery - Abstract
International audience; G protein-coupled receptors (GPCRs) signal through a limited number of G-protein pathways and play crucial roles in many biological processes. Studies of their in vivo functions have been hampered by the molecular and functional diversity of GPCRs and the paucity of ligands with specific signaling effects. To better compare the effects of activating different G-protein signaling pathways through ligand-induced or constitutive signaling, we developed a new series of RASSLs (receptors activated solely by synthetic ligands) that activate different G-protein signaling pathways. These RASSLs are based on the human 5-HT(4b) receptor, a GPCR with high constitutive G(s) signaling and strong ligand-induced G-protein activation of the G(s) and G(s/q) pathways. The first receptor in this series, 5-HT(4)-D(100)A or Rs1 (RASSL serotonin 1), is not activated by its endogenous agonist, serotonin, but is selectively activated by the small synthetic molecules GR113808, GR125487, and RO110-0235. All agonists potently induced G(s) signaling, but only a few (e.g., zacopride) also induced signaling via the G(q) pathway. Zacopride-induced G(q) signaling was enhanced by replacing the C-terminus of Rs1 with the C-terminus of the human 5-HT(2C) receptor. Additional point mutations (D(66)A and D(66)N) blocked constitutive G(s) signaling and lowered ligand-induced G(q) signaling. Replacing the third intracellular loop of Rs1 with that of human 5-HT(1A) conferred ligand-mediated G(i) signaling. This G(i)-coupled RASSL, Rs1.3, exhibited no measurable signaling to the G(s) or G(q) pathway. These findings show that the signaling repertoire of Rs1 can be expanded and controlled by receptor engineering and drug selection.
- Published
- 2007
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