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3 results on '"Dapeng Lei"'

1. Aberrant expression of PHLPP1 and PHLPP2 correlates with poor prognosis in patients with hypopharyngeal squamous cell carcinoma.

Author

Jieyu Zhou, Xuemin Yu, Juan Wang, Tao Li, Tong Jin, Dapeng Lei, and Xinliang Pan

Subjects
Medicine, Science
Abstract

The PHLPP (pleckstrin homology [PH] domain leucine rich repeat protein phosphatase) family, which represents a family of novel Ser/Thr protein phosphatases, is composed of 2 members: PHLPP1 and PHLPP2. PHLPPs partake in diverse cellular activities to exhibit their antitumor and metastasis suppressor functions. It is necessary to investigate the expression patterns of PHLPP1 and PHLPP2 in hypopharyngeal squamous cell carcinomas (HSCCs) and clarify their clinical significance. A total of 138 patients with primary HSCC who underwent curative surgical treatment as an initial treatment were enrolled in this study. A total of 138 HSCC specimens and 64 adjacent noncancerous mucosal epithelial tissues were collected. The expression levels of PHLPP1 and PHLPP2 were examined by quantitative reverse transcription polymerase chain reaction and immunohistochemistry assays. Correlations between clinicopathological parameters of the patients were further evaluated. PHLPP1 and PHLPP2 mRNA transcript levels were significantly lower in tumor samples than in paired adjacent nontumor mucosae (P

Published
2015
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2. Chloroquine-enhanced efficacy of cisplatin in the treatment of hypopharyngeal carcinoma in xenograft mice

Author

Dayu Liu, Xinliang Pan, Xiao-yan Yang, Dapeng Lei, Tong Jin, Xing-guo Zhao, and Ruijie Sun

Subjects
Pathology, medicine.medical_specialty, endocrine system diseases, medicine.medical_treatment, Drug Agonism, lcsh:Medicine, Mice, Nude, Apoptosis, Subcutaneous injection, Prostate cancer, Mice, Chloroquine, Cell Line, Tumor, medicine, Animals, Humans, lcsh:Science, Cisplatin, Chemotherapy, Mice, Inbred BALB C, Multidisciplinary, Hypopharyngeal Neoplasms, business.industry, lcsh:R, Autophagy, medicine.disease, Xenograft Model Antitumor Assays, Hepatocellular carcinoma, Cancer research, lcsh:Q, Female, business, medicine.drug, Research Article
Abstract

Hypopharyngeal squamous cell carcinoma (HSCC) has the worst prognosis among head and neck cancers. Cisplatin (DDP)-based chemotherapy is an important part of multimodal treatments. However, resistance to DDP severely impairs the effectiveness of chemotherapy for HSCC. Chloroquine (CQ) has been reported to enhance the effectiveness of chemotherapy and radiotherapy in liver, pancreas, breast, prostate and colon tumors, but it is unclear whether CQ could increase the efficacy of DDP for treating HSCC. We inoculated BALB/c nude mice with a subcutaneous injection of human hypopharyngeal FaDu cells to generate our animal model. Mice were randomly divided into 4 groups and treated with vehicle control, CQ (60 mg/kg/day), DDP (5 mg/kg/6 days), or a combination of DDP and CQ. Tumor growth and survival of the mice were monitored. We found that CQ inhibited autophagy and increased DDP-induced apoptosis in the xenograft mouse model. CQ enhanced the efficacy of DDP, resulting in decreased tumor growth and prolonged survival of the mice. To test whether blocking autophagy enhanced the efficacy of DDP, FaDu cells were infected with lentiviral shRNA to Beclin-1 and inoculated into the flanks of nude mice. Inhibition of autophagy markedly enhanced the DDP-induced antitumor effect. Our study suggests that the addition of CQ to DDP-based chemotherapy could be a potential therapeutic strategy for treating HSCC, and the inhibition of autophagy may contribute to chemotherapy sensitization in HSCC.

Published
2014

3. Aberrant Expression of Beclin-1 and LC3 Correlates with Poor Prognosis of Human Hypopharyngeal Squamous Cell Carcinoma

Author

Dayu Liu, Dapeng Lei, Li-Jie Ding, Xinliang Pan, Juan Wang, and Tong Jin

Subjects
Male, Pathology, medicine.medical_specialty, Regulator, lcsh:Medicine, Gene Expression, Biology, Metastasis, Hypopharyngeal Neoplasm, Gene expression, medicine, Humans, Clinical significance, lcsh:Science, Aged, Hypopharyngeal Neoplasms, Multidisciplinary, lcsh:R, Autophagy, Membrane Proteins, Middle Aged, Prognosis, medicine.disease, Survival Analysis, Hypopharynx, Apoptosis, Lymphatic Metastasis, Hepatocellular carcinoma, Multivariate Analysis, embryonic structures, Carcinoma, Squamous Cell, Cancer research, lcsh:Q, Beclin-1, Female, biological phenomena, cell phenomena, and immunity, Apoptosis Regulatory Proteins, Microtubule-Associated Proteins, Research Article
Abstract

Background Beclin-1, a key regulator of autophagy. Microtubule-associated protein 1 light chain 3 (LC3), is involved in autophagsome formation during autophagy. The autophagic genes beclin-1 and LC3 paly an important role in the development and progression of tumor. This study was designed to investigate the expression of beclin-1 and LC3 and to clarify their clinical significance in hypopharyngeal squamous cell carcinoma (HSCC). Methods Eighty-two surgical hypopharyngeal squamous cell carcinoma specimens and fifty-four adjacent non-cancerous mucosal epithelial tissues were obtained. Beclin-1 and LC3-II expression was examined by immunohistochemistry, real-time RT-PCR and Western blotting assays. Correlations with patient clinical characteristics and overall survival were evaluated. Results Beclin-1 was positively expressed in 42.7% (35/82) of HSCC specimens (adjacent non-cancerous tissues, 79.6%, 43/54; P

Published
2013

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