1. Anacardic acid enhances the proliferation of human ovarian cancer cells
- Author
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Yasuo Takano, Yang Zhao, Shuo Chen, Hua-chuan Zheng, Yin-Ling Xiu, and Wen-Feng Gou
- Subjects
lcsh:Medicine ,Apoptosis ,Biochemistry ,chemistry.chemical_compound ,Medicine and Health Sciences ,Pseudopodia ,Neoplasm Metastasis ,lcsh:Science ,Regulation of gene expression ,Ovarian Neoplasms ,Multidisciplinary ,Cell Death ,Cell Cycle ,Obstetrics and Gynecology ,Cell cycle ,Ovarian Cancer ,Gene Expression Regulation, Neoplastic ,Chemistry ,Real-time polymerase chain reaction ,Phenotype ,Oncology ,Cell Processes ,Physical Sciences ,Female ,Research Article ,Biology ,Real-Time Polymerase Chain Reaction ,Cell Growth ,Cell Line, Tumor ,Chemical Biology ,medicine ,Humans ,Neoplasm Invasiveness ,RNA, Messenger ,Cell Proliferation ,Cell growth ,lcsh:R ,Biology and Life Sciences ,Cancers and Neoplasms ,Cell Biology ,medicine.disease ,Anacardic acids ,Anacardic Acids ,chemistry ,Cell culture ,Immunology ,Cancer research ,Women's Health ,lcsh:Q ,Ovarian cancer ,Gynecological Tumors - Abstract
BACKGROUND: Anacardic acid (AA) is a mixture of 2-hydroxy-6-alkylbenzoic acid homologs. Certain antitumor activities of AA have been reported in a variety of cancers. However, the function of AA in ovarian cancer, to date, has remained unknown. METHODS: Ovarian cancer cell lines were exposed to AA, after which cell proliferation, apoptosis, invasion and migration assays were performed. Phalloidin staining was used to observe lamellipodia formation. Reverse transcription polymerase chain reaction (RT-PCR) and western blotting were used to assess the mRNA and protein expression levels of Phosphatidylinositol 3-kinase (PI3K), vascular endothelial growth factor (VEGF) and caspase 3. RESULTS: Our results showed that AA promotes ovarian cancer cell proliferation, inhibits late apoptosis, and induces cell migration and invasion, as well as lamellipodia formation. AA exposure significantly up-regulated PI3K and VEGF mRNA and protein expression, while, in contrast, it down-regulated caspase 3 mRNA and protein expression in comparison to untreated control cells. CONCLUSION: Taken together, our results demonstrate for the first time that AA may potentiate the proliferation, invasion, metastasis and lamellipodia formation in ovarian cancer cell lines via PI3K, VEGF and caspase 3 pathways.
- Published
- 2013