1. Differential microRNA expression in human placentas of term intra-uterine growth restriction that regulates target genes mediating angiogenesis and amino acid transport
- Author
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Thamotharan, Shanthie, Chu, Alison, Kempf, Katie, Janzen, Carla, Grogan, Tristan, Elashoff, David A, and Devaskar, Sherin U
- Subjects
Reproductive Medicine ,Biomedical and Clinical Sciences ,Medicinal and Biomolecular Chemistry ,Chemical Sciences ,Biotechnology ,Pediatric ,Perinatal Period - Conditions Originating in Perinatal Period ,Infant Mortality ,Genetics ,Preterm ,Low Birth Weight and Health of the Newborn ,Aetiology ,2.1 Biological and endogenous factors ,Amino Acids ,Blotting ,Western ,Female ,Fetal Growth Retardation ,Humans ,Infant ,Small for Gestational Age ,Male ,MicroRNAs ,Neovascularization ,Physiologic ,Oligonucleotide Array Sequence Analysis ,Placenta ,Pregnancy ,Real-Time Polymerase Chain Reaction ,Trophoblasts ,General Science & Technology - Abstract
Placental insufficiency leading to intrauterine growth restriction (IUGR) demonstrates perturbed gene expression affecting placental angiogenesis and nutrient transfer from mother to fetus. To understand the post-transcriptional mechanisms underlying such placental gene expression changes, our objective was to identify key non-coding microRNAs that express biological function. To this end, we initially undertook microarrays targeting microRNAs in a small sub-set of placentas of appropriate (AGA) versus small for gestational age (SGA) weight infants, and observed up-regulation of 97 miRs and down-regulation of 44 miRs in SGA versus AGA. In a larger cohort of samples (AGA, n = 21; SGA, n = 11; IUGR subset, n = 5), we validated by qRT-PCR differential expression of three specific microRNAs (miR-10b, -363 and -149) that target genes mediating angiogenesis and nutrient transfer. Validation yielded an increase in miR-10b and -363 expression of ~2.5-fold (p
- Published
- 2017