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1. Prospective observational study and serosurvey of SARS-CoV-2 infection in asymptomatic healthcare workers at a Canadian tertiary care center.

Author

Victor H Ferreira, Andrzej Chruscinski, Vathany Kulasingam, Trevor J Pugh, Tamara Dus, Brad Wouters, Amit Oza, Matthew Ierullo, Terrance Ku, Beata Majchrzak-Kita, Sonika T Humar, Ilona Bahinskaya, Natalia Pinzon, Jianhua Zhang, Lawrence E Heisler, Paul M Krzyzanowski, Bernard Lam, Ilinca M Lungu, Dorin Manase, Krista M Pace, Pouria Mashouri, Michael Brudno, Michael Garrels, Tony Mazzulli, Myron Cybulsky, Atul Humar, and Deepali Kumar

Subjects
Medicine, Science
Abstract

Health care workers (HCWs) are at higher risk for SARS-CoV-2 infection and may play a role in transmitting the infection to vulnerable patients and members of the community. This is particularly worrisome in the context of asymptomatic infection. We performed a cross-sectional study looking at asymptomatic SARS-CoV-2 infection in HCWs. We screened asymptomatic HCWs for SARS-CoV-2 via PCR. Complementary viral genome sequencing was performed on positive swab specimens. A seroprevalence analysis was also performed using multiple assays. Asymptomatic health care worker cohorts had a combined swab positivity rate of 29/5776 (0.50%, 95%CI 0.32-0.75) relative to a comparative cohort of symptomatic HCWs, where 54/1597 (3.4%) tested positive for SARS-CoV-2 (ratio of symptomatic to asymptomatic 6.8:1). SARS-CoV-2 seroprevalence among 996 asymptomatic HCWs with no prior known exposure to SARS-CoV-2 was 1.4-3.4%, depending on assay. A novel in-house Coronavirus protein microarray showed differing SARS-CoV-2 protein reactivities and helped define likely true positives vs. suspected false positives. Our study demonstrates the utility of routine screening of asymptomatic HCWs, which may help to identify a significant proportion of infections.

Published
2021
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2. Prospective observational study and serosurvey of SARS-CoV-2 infection in asymptomatic healthcare workers at a Canadian tertiary care center

Author

Vathany Kulasingam, Victor H Ferreira, Michael Brudno, Atul Humar, Bernard Lam, Myron I. Cybulsky, Trevor J. Pugh, Krista M. Pace, Natalia Pinzon, Matthew Ierullo, Ilinca Lungu, Brad Wouters, Sonika T. Humar, Paul M. Krzyzanowski, Tamara Dus, Michael Garrels, Amit M. Oza, Beata Majchrzak-Kita, Andrzej Chruscinski, Ilona Bahinskaya, Pouria Mashouri, Deepali Kumar, Tony Mazzulli, Terrance Ku, Dorin Manase, Jianhua Zhang, and Lawrence E. Heisler

Subjects
RNA viruses, Viral Diseases, Pulmonology, Coronaviruses, Microarrays, Physiology, viruses, Biochemistry, Serology, Tertiary Care Centers, Medical Conditions, Seroepidemiologic Studies, Immune Physiology, Epidemiology, Health care, Medical Personnel, Enzyme-Linked Immunoassays, Asymptomatic Infections, Pathology and laboratory medicine, Virus Testing, Immune System Proteins, Multidisciplinary, virus diseases, Medical microbiology, Professions, Infectious Diseases, Bioassays and Physiological Analysis, COVID-19 Nucleic Acid Testing, Viruses, Cohort, Medicine, SARS CoV 2, Pathogens, medicine.symptom, Research Article, Canada, medicine.medical_specialty, SARS coronavirus, Health Personnel, Science, Immunology, Context (language use), Research and Analysis Methods, Microbiology, Asymptomatic, Antibodies, COVID-19 Serological Testing, Respiratory Disorders, Diagnostic Medicine, Internal medicine, medicine, Humans, Seroprevalence, Immunoassays, Medicine and health sciences, Biology and life sciences, business.industry, Organisms, Viral pathogens, Proteins, COVID-19, Covid 19, Microbial pathogens, Respiratory Infections, People and Places, Immunologic Techniques, Population Groupings, business
Abstract

Health care workers (HCWs) are at higher risk for SARS-CoV-2 infection and may play a role in transmitting the infection to vulnerable patients and members of the community. This is particularly worrisome in the context of asymptomatic infection. We performed a cross-sectional study looking at asymptomatic SARS-CoV-2 infection in HCWs. We screened asymptomatic HCWs for SARS-CoV-2 via PCR. Complementary viral genome sequencing was performed on positive swab specimens. A seroprevalence analysis was also performed using multiple assays. Asymptomatic health care worker cohorts had a combined swab positivity rate of 29/5776 (0.50%, 95%CI 0.32–0.75) relative to a comparative cohort of symptomatic HCWs, where 54/1597 (3.4%) tested positive for SARS-CoV-2 (ratio of symptomatic to asymptomatic 6.8:1). SARS-CoV-2 seroprevalence among 996 asymptomatic HCWs with no prior known exposure to SARS-CoV-2 was 1.4–3.4%, depending on assay. A novel in-house Coronavirus protein microarray showed differing SARS-CoV-2 protein reactivities and helped define likely true positives vs. suspected false positives. Our study demonstrates the utility of routine screening of asymptomatic HCWs, which may help to identify a significant proportion of infections.

Published
2021

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