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18 results on '"Bergquist, Jonas"'

2. The proteome of perilymph in patients with vestibular schwannoma. A possibility to identify biomarkers for tumor associated hearing loss?

Author

Edvardsson Rasmussen, Jesper, Laurell, Göran, Rask-Andersen, Helge, Bergquist, Jonas, and Eriksson, Per Olof

Subjects
Male, Metabolic Processes, Computer and Information Sciences, Proteome, alpha-2-HS-Glycoprotein, Proteomes, Immunology, Oto-rino-laryngologi, lcsh:Medicine, Perilymph, Otology, Surgical and Invasive Medical Procedures, Deafness, Biochemistry, Tandem Mass Spectrometry, Medicine and Health Sciences, Ontologies, otorhinolaryngologic diseases, Humans, Hearing Loss, lcsh:Science, Hearing Disorders, Immune Response, Chromatography, High Pressure Liquid, Data Management, lcsh:R, Biology and Life Sciences, Proteins, Metabolism, Otorhinolaryngology, Ears, Inner Ear, Linear Models, Female, lcsh:Q, sense organs, Anatomy, Head, Biomarkers, Neurilemmoma, Research Article
Abstract

Background Due to the surrounding bone, the human inner ear is relatively inaccessible and difficult to reach for cellular and molecular analyses. However, these types of investigations are needed to better understand the etiology, pathophysiology and progression of several inner ear disorders. Moreover, the fluid from the inner ear cannot be sampled for micro-chemical analyses from healthy individuals in vivo. Therefore, in the present paper, we studied patients with vestibular schwannoma (VS) undergoing trans-labyrinthine surgery (TLS). Our primary aim was to identify perilymph proteins in patients with VS on an individual level. Our second aim was to investigate the proteins identified at a functional level and our final aim was to search for biological markers for tumor-associated hearing loss and tumor diameter. Methods and findings Sixteen patients underwent TLS for sporadic VS. Perilymph was aspirated through the round window before opening the labyrinth. One sample was contaminated and excluded resulting in 15 usable samples. Perilymph samples were analyzed with an online tandem LTQ-Orbitrap mass spectrometer. Data were analyzed with MaxQuant software to identify the total number of proteins and to quantify proteins in individual samples. Protein function was analyzed using the PANTHER Overrepresentation tool. Associations between perilymph protein content, clinical parameters, tumor-associated hearing loss and tumor diameter were assessed using Random Forest and Boruta. In total, 314 proteins were identified; 60 in all 15 patients and 130 proteins only once in 15 patients. Ninety-one proteins were detected in at least 12 out of 15 patients. Random Forest followed by Boruta analysis confirmed that alpha-2-HS-glycoprotein (P02765) was an independent variable for tumor-associated hearing loss. In addition, functional analysis showed that numerous processes were significantly increased in the perilymph. The top three enriched biological processes were: 1) secondary metabolic processes; 2) complement activation and 3) cell recognition. Conclusions The proteome of perilymph in patients with vestibular schwannoma has an inter-individual stable section. However, even in a cohort with homogenous disease, the variation between individuals represented the majority of the detected proteins. Alpha-2-HS-glycoprotein, P02765, was shown to be an independent variable for tumor-associated hearing loss, a finding that needs to be verified in other studies. In pathway analysis perilymph had highly enriched functions, particularly in terms of increased immune and metabolic processes.

Published
2018

3. Combined lipidomic and proteomic analysis of isolated human islets exposed to palmitate reveals time-dependent changes in insulin secretion and lipid metabolism

Author

Roomp, Kirsten, primary, Kristinsson, Hjalti, additional, Schvartz, Domitille, additional, Ubhayasekera, Kumari, additional, Sargsyan, Ernest, additional, Manukyan, Levon, additional, Chowdhury, Azazul, additional, Manell, Hannes, additional, Satagopam, Venkata, additional, Groebe, Karlfried, additional, Schneider, Reinhard, additional, Bergquist, Jonas, additional, Sanchez, Jean-Charles, additional, and Bergsten, Peter, additional

Published
2017
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6. Analysis of the Cerebrospinal Fluid Proteome in Alzheimer's Disease

Author

Khoonsari, Payam Emami, primary, Häggmark, Anna, additional, Lönnberg, Maria, additional, Mikus, Maria, additional, Kilander, Lena, additional, Lannfelt, Lars, additional, Bergquist, Jonas, additional, Ingelsson, Martin, additional, Nilsson, Peter, additional, Kultima, Kim, additional, and Shevchenko, Ganna, additional

Published
2016
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7. A Multiplex Protein Panel Applied to Cerebrospinal Fluid Reveals Three New Biomarker Candidates in ALS but None in Neuropathic Pain Patients

Author

Lind, Anne-Li, primary, Wu, Di, additional, Freyhult, Eva, additional, Bodolea, Constantin, additional, Ekegren, Titti, additional, Larsson, Anders, additional, Gustafsson, Mats G., additional, Katila, Lenka, additional, Bergquist, Jonas, additional, Gordh, Torsten, additional, Landegren, Ulf, additional, and Kamali-Moghaddam, Masood, additional

Published
2016
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8. Eye Movements in Ephedrone-Induced Parkinsonism

Author

Bonnet, Cecilia, primary, Rusz, Jan, additional, Megrelishvili, Marika, additional, Sieger, Tomáš, additional, Matoušková, Olga, additional, Okujava, Michael, additional, Brožová, Hana, additional, Nikolai, Tomáš, additional, Hanuška, Jaromír, additional, Kapianidze, Mariam, additional, Mikeladze, Nina, additional, Botchorishvili, Nazi, additional, Khatiashvili, Irine, additional, Janelidze, Marina, additional, Serranová, Tereza, additional, Fiala, Ondřej, additional, Roth, Jan, additional, Bergquist, Jonas, additional, Jech, Robert, additional, Rivaud-Péchoux, Sophie, additional, Gaymard, Bertrand, additional, and Růžička, Evžen, additional

Published
2014
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10. Gray Matter Is Targeted in First-Attack Multiple Sclerosis

Author

Schutzer, Steven E., primary, Angel, Thomas E., additional, Liu, Tao, additional, Schepmoes, Athena A., additional, Xie, Fang, additional, Bergquist, Jonas, additional, Vécsei, László, additional, Zadori, Denes, additional, Camp, David G., additional, Holland, Bart K., additional, Smith, Richard D., additional, and Coyle, Patricia K., additional

Published
2013
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16. Distinct Cerebrospinal Fluid Proteomes Differentiate Post-Treatment Lyme Disease from Chronic Fatigue Syndrome

Author

Schutzer, Steven E., primary, Angel, Thomas E., additional, Liu, Tao, additional, Schepmoes, Athena A., additional, Clauss, Therese R., additional, Adkins, Joshua N., additional, Camp, David G., additional, Holland, Bart K., additional, Bergquist, Jonas, additional, Coyle, Patricia K., additional, Smith, Richard D., additional, Fallon, Brian A., additional, and Natelson, Benjamin H., additional

Published
2011
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17. Establishing the Proteome of Normal Human Cerebrospinal Fluid

Author

Schutzer, Steven E., primary, Liu, Tao, additional, Natelson, Benjamin H., additional, Angel, Thomas E., additional, Schepmoes, Athena A., additional, Purvine, Samuel O., additional, Hixson, Kim K., additional, Lipton, Mary S., additional, Camp, David G., additional, Coyle, Patricia K., additional, Smith, Richard D., additional, and Bergquist, Jonas, additional

Published
2010
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18. Production of Squalene in Synechocystis sp. PCC 6803.

Author

Englund, Elias, Pattanaik, Bagmi, Ubhayasekera, Sarojini Jayantha K., Stensjö, Karin, Bergquist, Jonas, and Lindberg, Pia

Subjects
SYNECHOCYSTIS, SQUALENE, ATMOSPHERIC carbon dioxide, PHOTOSYNTHESIS, PETROLEUM, CHEMICAL industry, RENEWABLE energy sources
Abstract

In recent years, there has been an increased interest in the research and development of sustainable alternatives to fossil fuels. Using photosynthetic microorganisms to produce such alternatives is advantageous, since they can achieve direct conversion of carbon dioxide from the atmosphere into the desired product, using sunlight as the energy source. Squalene is a naturally occurring 30-carbon isoprenoid, which has commercial use in cosmetics and in vaccines. If it could be produced sustainably on a large scale, it could also be used instead of petroleum as a raw material for fuels and as feedstock for the chemical industry. The unicellular cyanobacterium Synechocystis PCC 6803 possesses a gene, slr2089, predicted to encode squalene hopene cyclase (Shc), an enzyme converting squalene into hopene, the substrate for forming hopanoids. Through inactivation of slr2089 (shc), we explored the possibility to produce squalene using cyanobacteria. The inactivation led to accumulation of squalene, to a level over 70 times higher than in wild type cells, reaching 0.67 mg OD750−1 L−1. We did not observe any significant growth deficiency in the Δshc strain compared to the wild type Synechocystis, even at high light conditions, suggesting that the observed squalene accumulation was not detrimental to growth, and that formation of hopene by Shc is not crucial for growth under normal conditions, nor for high-light stress tolerance. Effects of different light intensities and growth stages on squalene accumulation in the Δshc strain were investigated. We also identified a gene, sll0513, as a putative squalene synthase in Synechocystis, and verified its function by inactivation. In this work, we show that it is possible to use the cyanobacterium Synechocystis to generate squalene, a hydrocarbon of commercial interest and a potential biofuel. We also report the first identification of a squalene hopene cyclase, and the second identification of squalene synthase, in cyanobacteria. [ABSTRACT FROM AUTHOR]

Published
2014
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