1. Characterization of Salmonella enterica isolates causing bacteremia in Lima, Peru, using multiple typing methods
- Author
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Claudia Silva, Laura Betancor, Coralith García, Lizeth Astocondor, Noemí Hinostroza, Julieta Bisio, Javier Rivera, Lucía Perezgasga, Victoria Pérez Escanda, Lucía Yim, Jan Jacobs, Francisco García-Del Portillo, SalmoIber CYTED Network, José A Chabalgoity, and José L Puente
- Subjects
0301 basic medicine ,Serotype ,Bacterial Diseases ,Salmonella ,Salmonellosis ,Physiology ,lcsh:Medicine ,Bacteremia ,Artificial Gene Amplification and Extension ,medicine.disease_cause ,Pathology and Laboratory Medicine ,Polymerase Chain Reaction ,Medicine and Health Sciences ,lcsh:Science ,Multidisciplinary ,Salmonella enterica ,Bacteremia/microbiology ,RAPD ,Bacterial Pathogens ,Body Fluids ,Infectious Diseases ,Blood ,Medical Microbiology ,Pathogens ,Anatomy ,Random amplified polymorphic DNA technique ,Research Article ,Salmonella enterica/genetics/isolation & purification ,030106 microbiology ,Biology ,Research and Analysis Methods ,Microbiology ,03 medical and health sciences ,Enterobacteriaceae ,Microbial Control ,Multiplex polymerase chain reaction ,medicine ,Animals ,Humans ,Typing ,purl.org/pe-repo/ocde/ford#1.06.01 [https] ,Molecular Biology Techniques ,Microbial Pathogens ,Molecular Biology ,Pharmacology ,Bacteria ,lcsh:R ,Organisms ,Biology and Life Sciences ,biology.organism_classification ,Multiple drug resistance ,030104 developmental biology ,Antibiotic Resistance ,Multilocus sequence typing ,bacteria ,lcsh:Q ,Antimicrobial Resistance ,Multiplex Polymerase Chain Reaction - Abstract
In this study, different molecular typing tools were applied to characterize 95 Salmonella enterica blood isolates collected between 2008 and 2013 from patients at nine public hospitals in Lima, Peru. Combined results of multiplex PCR serotyping, two- and seven-loci multilocus sequence typing (MLST) schemes, serotyping, IS200 amplification and RAPD fingerprints, showed that these infections were caused by eight different serovars: Enteritidis, Typhimurium, Typhi, Choleraesuis, Dublin, Paratyphi A, Paratyphi B and Infantis. Among these, Enteritidis, Typhimurium and Typhi were the most prevalent, representing 45, 36 and 11% of the isolates, respectively. Most isolates (74%) were not resistant to ten primarily used antimicrobial drugs; however, 37% of the strains showed intermediate susceptibility to ciprofloxacin (ISC). Antimicrobial resistance integrons were carried by one Dublin (dfra1 and aadA1) and two Infantis (aadA1) isolates. The two Infantis isolates were multidrug resistant and harbored a large megaplasmid. Amplification of spvC and spvRA regions showed that all Enteritidis (n = 42), Typhimurium (n = 34), Choleraesuis (n = 3) and Dublin (n = 1) isolates carried the Salmonella virulence plasmid (pSV). We conclude that the classic serotyping method can be substituted by the multiplex PCR and, when necessary, sequencing of only one or two loci of the MLST scheme is a valuable tool to confirm the results. The effectiveness and feasibility of different typing tools is discussed.
- Published
- 2017