1. Usability of graphene oxide as a mycotoxin binder: In vitro study
- Author
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Jiri Skladanka, Andrea Ridošková, Sylvie Skalickova, Eva Venusova, Tereza Aulichova, and Pavel Horky
- Subjects
Aflatoxin ,Aflatoxin B1 ,Hydrolases ,Physiology ,01 natural sciences ,Physical Chemistry ,Biochemistry ,law.invention ,chemistry.chemical_compound ,law ,Lipases ,Zearalenone ,Materials ,Triticum ,0303 health sciences ,Multidisciplinary ,Eukaryota ,food and beverages ,Proteases ,Plants ,Enzymes ,Chemistry ,Physical Sciences ,Wheat ,Sorption ,Medicine ,Digestion ,Graphite ,Digestive Enzymes ,Research Article ,Science ,Materials Science ,Oxide ,Food Contamination ,In Vitro Techniques ,03 medical and health sciences ,Adsorption ,Animals ,Humans ,Gastric lipase ,Grasses ,Mycotoxin ,030304 developmental biology ,Graphene ,010401 analytical chemistry ,Organisms ,Biology and Life Sciences ,Zea ,Proteins ,Mycotoxins ,Animal Feed ,0104 chemical sciences ,Nanostructures ,chemistry ,Gastrointestinal Absorption ,Enzymology ,Serine Proteases ,Physiological Processes ,Trichothecenes ,Nuclear chemistry - Abstract
Mycotoxin management in agriculture is an essential challenge for maintaining the health of both animals and humans. Choosing the right adsorbent is still a question for many breeders and an important criterion for feed manufacturers. New adsorbents are still being sought. Graphene oxide is a promising material in the field of nanotechnology, which excels in its adsorption properties. Presented in vitro study investigates graphene oxide for the binding of mycotoxins from crushed wheat. The results show that graphene oxide has an adsorption capacity for aflatoxin 0.045 mg/g, zearalenone 0.53 mg/g and deoxynivalenol 1.69 mg/g at 37° C. In vitro simulation of crushed wheat digestion showed rapid adsorption during the gastric phase. Of the minerals, Mg, Cu and Zn were the most adsorbed. The applied dose of graphene oxide of 10 mg/g caused only a slight inhibition of the digestive enzymes α-amylase and trypsin compared to pepsin and gastric lipase. In vitro results indicated the suitability of graphene oxide in the adsorption of the aflatoxin, zearalenone and deoxynivalenol.
- Published
- 2020