1. Performance of Four Transport and Storage Systems for Molecular Detection of Multidrug-Resistant Tuberculosis.
- Author
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Rabodoarivelo MS, Imperiale B, Andrianiavomikotroka R, Brandao A, Kumar P, Singh S, Ferrazoli L, Morcillo N, Rasolofo V, Palomino JC, Vandamme P, and Martin A
- Subjects
- Antitubercular Agents pharmacology, Argentina, Bacterial Typing Techniques, Bacteriological Techniques instrumentation, Brazil, DNA, Bacterial genetics, Ethanol, Filtration instrumentation, Genotype, Genotyping Techniques, Glass, Humans, India, Microbial Sensitivity Tests methods, Mycobacterium tuberculosis drug effects, Mycobacterium tuberculosis genetics, Paper, Polymerase Chain Reaction instrumentation, Polymerase Chain Reaction methods, Postal Service, Preservation, Biological instrumentation, Retrospective Studies, Ribotyping instrumentation, Ribotyping methods, Sensitivity and Specificity, Specimen Handling methods, Bacteriological Techniques methods, DNA, Bacterial isolation & purification, Drug Resistance, Multiple, Bacterial genetics, Molecular Diagnostic Techniques methods, Mycobacterium tuberculosis isolation & purification, Preservation, Biological methods, Specimen Handling instrumentation, Sputum microbiology, Transportation instrumentation, Tuberculosis, Multidrug-Resistant diagnosis
- Abstract
Background: Detection of drug-resistant tuberculosis is essential for the control of the disease but it is often hampered by the limitation of transport and storage of samples from remote locations to the reference laboratory. We performed a retrospective field study to evaluate the performance of four supports enabling the transport and storage of samples to be used for molecular detection of drug resistance using the GenoType MTBDRplus., Methods: Two hundred Mycobacterium tuberculosis strains were selected and spotted on slides, FTA cards, GenoCards, and in ethanol. GenoType MTBDRplus was subsequently performed with the DNA extracted from these supports. Sensitivity and specificity were calculated and compared to the results obtained by drug susceptibility testing., Results: For all supports, the overall sensitivity and specificity for detection of resistance to RIF was between 95% and 100%, and for INH between 95% and 98%., Conclusion: The four transport and storage supports showed a good sensitivity and specificity for the detection of resistance to RIF and INH in M. tuberculosis strains using the GenoType MTBDRplus. These supports can be maintained at room temperature and could represent an important alternative cost-effective method useful for rapid molecular detection of drug-resistant TB in low-resource settings.
- Published
- 2015
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