1. Nicotine treatment regulates PD-L1 and PD-L2 expression via inhibition of Akt pathway in HER2-type breast cancer cells
- Author
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Masanori A. Murayama, Erika Takada, Kenji Takai, Nagisa Arimitsu, Jun Shimizu, Tomoko Suzuki, and Noboru Suzuki
- Subjects
Nicotinic Acetylcholine Receptors ,Cell signaling ,Receptor, ErbB-2 ,Anti-Addiction Drug Therapy ,Cancer Treatment ,Biochemistry ,B7-H1 Antigen ,Breast Tumors ,Medicine and Health Sciences ,AKT signaling cascade ,Phosphorylation ,skin and connective tissue diseases ,Staining ,Multidisciplinary ,Pharmaceutics ,Signaling cascades ,Specimen preparation and treatment ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Chemistry ,Oncology ,Physical Sciences ,Medicine ,Female ,Immunotherapy ,Research Article ,Signal Transduction ,Neurological Drug Therapy ,Nicotine ,Transmembrane Receptors ,Cell Survival ,Science ,Immunology ,Breast Neoplasms ,Cancer Immunotherapy ,Alkaloids ,Drug Therapy ,Nicotine Replacement Therapy ,Cell Line, Tumor ,Breast Cancer ,Humans ,Cell Proliferation ,Dose-Response Relationship, Drug ,Chemical Compounds ,DAPI staining ,Cancers and Neoplasms ,Biology and Life Sciences ,Proteins ,Cell Biology ,Programmed Cell Death 1 Ligand 2 Protein ,Research and analysis methods ,Acetylcholine Receptors ,Nuclear staining ,Clinical Immunology ,Clinical Medicine ,Proto-Oncogene Proteins c-akt - Abstract
The immune checkpoint molecules such as PD-L1 and PD-L2 have a substantial contribution to cancer immunotherapy including breast cancer. Microarray expression profiling identified several molecular subtypes, namely luminal-type (with a good-prognosis), HER2-type (with an intermediate-prognosis), and triple-negative breast cancer (TNBC)-type (with a poor-prognosis). We found that PD-L1 and PD-L2 mRNA expressions were highly expressed in TNBC-type cell lines (HCC1937, MDA-MB-231), moderately expressed in HER2-type cell line (SK-BR-3), and poorly expressed in luminal-type cell lines (MDA-MB-361, MCF7). The PD-L1 and PD-L2 expression in SK-BR-3 cells, but not those in HCC1937 and MDA-MB-231 cells, decreased by nicotine stimulation in a dose-dependent manner. In addition, nicotine treatment decreased the phosphorylation of Akt in SK-BR-3 cells, but not in other cell lines. These results show that nicotine regulates the expression of immune checkpoint molecules, PD-L1 and PD-L2, via inhibition of Akt phosphorylation. This findings may provide the new therapeutic strategies for the treatment of breast cancer.
- Published
- 2022