1. CD117+ Dendritic and Mast Cells Are Dependent on RasGRP4 to Function as Accessory Cells for Optimal Natural Killer Cell-Mediated Responses to Lipopolysaccharide.
- Author
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Zhou, Saijun, Tanaka, Kumiko, O’Keeffe, Meredith, Qi, Miao, El-Assaad, Fatima, Weaver, James C., Chen, Gang, Weatherall, Christopher, Wang, Ying, Giannakopoulos, Bill, Chen, Liming, Yu, DeMint, Hamilton, Matthew J., Wensing, Lislaine A., Stevens, Richard L., and Krilis, Steven A.
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DENDRITIC cells , *MAST cells , *KILLER cells , *C-kit protein , *LIPOPOLYSACCHARIDES , *G proteins - Abstract
Ras guanine nucleotide-releasing protein-4 (RasGRP4) is an evolutionarily conserved calcium-regulated, guanine nucleotide exchange factor and diacylglycerol/phorbol ester receptor. While an important intracellular signaling protein for CD117+ mast cells (MCs), its roles in other immune cells is less clear. In this study, we identified a subset of in vivo-differentiated splenic CD117+ dendritic cells (DCs) in wild-type (WT) C57BL/6 mice that unexpectedly contained RasGRP4 mRNA and protein. In regard to the biologic significance of these data to innate immunity, LPS-treated splenic CD117+ DCs from WT mice induced natural killer (NK) cells to produce much more interferon-γ (IFN-γ) than comparable DCs from RasGRP4-null mice. The ability of LPS-responsive MCs to cause NK cells to increase their expression of IFN-γ was also dependent on this intracellular signaling protein. The discovery that RasGRP4 is required for CD117+ MCs and DCs to optimally induce acute NK cell-dependent immune responses to LPS helps explain why this signaling protein has been conserved in evolution. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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